FastQCFastQC Report
Fri 10 Feb 2017
SRR2031828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119389
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39220.350369710618918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36040.3219613557038706No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT29950.26755667600807226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.19126505620476886No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.060.3172231
GTATCAA32350.038.091661
ATCAACG37550.032.1299363
TCAACGC37550.032.1299364
CAACGCA38100.031.6661155
AACGCAG39350.030.6602026
TATCAAC40200.030.0119172
TAGTACG856.407726E-427.9406914
ACGCAGA43850.027.5137777
CGCAGAG43950.027.2350228
GCAGAGT50000.023.6545899
CAGAGTA48300.022.61865410-11
GTACATG21250.022.6134511
GAGTACT38100.022.44055412-13
TGGTATC8100.021.6971532
AGAGTAC45150.021.0406110-11
TACATGG21700.021.013462
GTACTTT43950.019.3455114-15
ACATGGG21150.019.3140853
AGTACTT41300.019.26419312-13
Produced by FastQC (version 0.11.5)