FastQCFastQC Report
Fri 10 Feb 2017
SRR2031827_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031827_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413370
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.38996540629460286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.3427921716621912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.21191668481021844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.053.146311
GTATCAA13400.042.536881
TCAACGC15850.035.3624154
ATCAACG16100.034.813313
CAACGCA16150.034.705535
AACGCAG16450.033.783856
TATCAAC17250.032.7677842
ACGCAGA17900.030.5164497
CGCAGAG17750.030.5067338
GCAGAGT19350.028.2296879
GCGTGCG1000.00165195923.7497589
AGAGTAC17550.023.27611710-11
CAGAGTA18950.022.80979310-11
GAGTACT14200.022.24449212-13
GTCATAC1100.002874453821.5906891
GTACATG9500.020.9997841
TACATGG9400.020.7178752
AGTACTT15350.020.57796712-13
CGCAGAA1404.8448428E-420.3569369
ACATGGG9400.020.212563
Produced by FastQC (version 0.11.5)