##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031827_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 413370 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57706655054793 34.0 31.0 34.0 31.0 34.0 2 32.706812298908964 34.0 33.0 34.0 31.0 34.0 3 32.78073396714807 34.0 33.0 34.0 31.0 34.0 4 35.92913612502117 37.0 37.0 37.0 35.0 37.0 5 35.95951568812444 37.0 37.0 37.0 35.0 37.0 6 36.033384135278325 37.0 37.0 37.0 35.0 37.0 7 36.056092604688295 37.0 37.0 37.0 35.0 37.0 8 36.058818975736024 37.0 37.0 37.0 35.0 37.0 9 37.902407044536375 39.0 39.0 39.0 37.0 39.0 10-11 37.943805791421724 39.0 39.0 39.0 37.0 39.0 12-13 37.92271935554104 39.0 39.0 39.0 37.0 39.0 14-15 39.518248784382024 41.0 40.0 41.0 37.5 41.0 16-17 39.45246268476184 41.0 40.0 41.0 37.0 41.0 18-19 39.45927014539033 41.0 40.0 41.0 37.0 41.0 20-21 39.40180830732758 41.0 40.0 41.0 37.0 41.0 22-23 39.30958947190169 41.0 40.0 41.0 37.0 41.0 24-25 39.28711203038441 41.0 40.0 41.0 37.0 41.0 26-27 39.16381208118635 41.0 40.0 41.0 36.0 41.0 28-29 39.04038028884534 41.0 39.5 41.0 36.0 41.0 30-31 38.875055035440404 41.0 39.0 41.0 35.0 41.0 32-33 38.72755521687593 41.0 39.0 41.0 35.0 41.0 34-35 38.5328543435663 41.0 39.0 41.0 35.0 41.0 36-37 38.40013668142342 40.5 38.0 41.0 35.0 41.0 38-39 38.2056680455766 40.0 38.0 41.0 34.0 41.0 40-41 38.11047971550911 40.0 38.0 41.0 34.0 41.0 42-43 37.92731693156252 40.0 38.0 41.0 33.0 41.0 44-45 38.043109079033314 40.0 38.0 41.0 34.0 41.0 46-47 37.88655078984929 40.5 38.0 41.0 33.0 41.0 48-49 37.70667682705567 40.0 38.0 41.0 33.0 41.0 50-51 36.65525316302586 39.0 36.5 40.0 31.5 40.5 52-53 36.671000556402255 39.5 37.5 40.0 31.0 40.5 54-55 37.42590173452355 40.0 38.0 41.0 32.0 41.0 56-57 37.39878075332027 40.0 37.5 41.0 32.0 41.0 58-59 37.27551346251542 40.0 37.0 41.0 32.0 41.0 60-61 37.08531219004766 40.0 36.5 41.0 31.5 41.0 62-63 36.82737499092822 40.0 36.0 41.0 31.0 41.0 64-65 36.51959987420471 39.0 35.5 41.0 30.5 41.0 66-67 36.24256598204998 39.0 35.0 41.0 30.0 41.0 68-69 35.9104664102378 38.5 35.0 41.0 30.0 41.0 70-71 35.54702566707792 37.0 35.0 40.5 29.0 41.0 72-73 35.137513607663834 37.0 35.0 39.5 29.0 41.0 74-75 34.772051672835474 36.0 35.0 39.0 29.0 41.0 76-77 34.39048189273532 36.0 35.0 39.0 28.5 40.5 78-79 34.000959189104194 35.5 35.0 37.0 28.0 39.0 80-81 33.64228899049278 35.0 35.0 37.0 27.0 39.0 82-83 33.34477707622711 35.0 34.5 36.5 26.5 39.0 84-85 33.0223818854779 35.0 34.0 36.0 26.0 37.0 86-87 32.763118997508286 35.0 34.0 36.0 25.5 37.0 88-89 32.56771173524929 35.0 34.0 35.5 25.0 37.0 90-91 32.37544209787842 35.0 34.0 35.0 25.0 36.0 92-93 32.23808573433002 35.0 34.0 35.0 24.5 36.0 94-95 32.07494254541936 35.0 34.0 35.0 24.0 36.0 96-97 31.975345332268912 35.0 34.0 35.0 23.5 36.0 98-99 31.85607567070663 35.0 34.0 35.0 23.0 35.0 100-101 30.38452475990033 34.0 30.5 35.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2738655578733429 1101 2 0.2752975030152456 1101 3 0.36564510389263916 1101 4 0.309142390962009 1101 5 0.2789741986465586 1101 6 0.32575935164947367 1101 7 0.2891728458922955 1101 8 0.3604696753206156 1101 9 0.3468806364747081 1101 10-11 0.43370431038928814 1101 12-13 0.33632599689141074 1101 14-15 0.24676566712074077 1101 16-17 0.32551826858216515 1101 18-19 0.3180603281618417 1101 20-21 0.3153450568616236 1101 22-23 0.3735844512891049 1101 24-25 0.1722624383807272 1101 26-27 0.04713908922850152 1101 28-29 0.17882165372810022 1101 30-31 0.1360817633115019 1101 32-33 0.006416749560379742 1101 34-35 0.24195268547734372 1101 36-37 0.22174933080221848 1101 38-39 0.3095003046270435 1101 40-41 0.19818619707466922 1101 42-43 0.4814983014252192 1101 44-45 0.47402173647567025 1101 46-47 0.6671208394807238 1101 48-49 1.0561057691228868 1101 50-51 1.2451410896940942 1101 52-53 0.8875278504014616 1101 54-55 0.7662677225446757 1101 56-57 0.8279220403250278 1101 58-59 0.7935158532431146 1101 60-61 0.9833584695899376 1101 62-63 0.9620941326168122 1101 64-65 0.8549960612596834 1101 66-67 0.6670283715855305 1101 68-69 0.8467674126482905 1101 70-71 0.6407606538037314 1101 72-73 0.7880395468736268 1101 74-75 0.6890112633884442 1101 76-77 0.5431719948352409 1101 78-79 0.5637725208615336 1101 80-81 0.4878044033021567 1101 82-83 0.48406564796519547 1101 84-85 0.4728257781049692 1101 86-87 0.6736857863660006 1101 88-89 0.5448135249056705 1101 90-91 0.5198789245587179 1101 92-93 0.5321493196197622 1101 94-95 0.7746225329197998 1101 96-97 0.5860358193391093 1101 98-99 0.47256299484120845 1101 100-101 0.6657624833348095 1102 1 0.1685367898393011 1102 2 0.08488463153063464 1102 3 0.08303424025325512 1102 4 0.21541524516052846 1102 5 0.0329632951162111 1102 6 -0.02129204121004591 1102 7 0.07755127817738128 1102 8 0.26574525540173255 1102 9 0.32484087847989684 1102 10-11 0.31704817050497525 1102 12-13 0.41932413531305457 1102 14-15 0.39259207493936543 1102 16-17 0.4326753724981245 1102 18-19 0.3628213147983672 1102 20-21 0.3321989894458923 1102 22-23 0.3841056932278093 1102 24-25 0.25409709033206695 1102 26-27 0.3696658494173235 1102 28-29 0.32320201407647886 1102 30-31 0.30297558686046955 1102 32-33 0.19306491125175285 1102 34-35 0.32626390148691087 1102 36-37 0.21966336577329315 1102 38-39 0.33504714326809903 1102 40-41 0.3136040549298684 1102 42-43 0.47008782339848665 1102 44-45 0.42507219854601175 1102 46-47 0.30139548877265554 1102 48-49 0.0057517368112058875 1102 50-51 0.16919866565314123 1102 52-53 0.40410753260344023 1102 54-55 0.4055536742766108 1102 56-57 0.4272671636545837 1102 58-59 0.24807339595968614 1102 60-61 0.45527689256713444 1102 62-63 0.19194919544507627 1102 64-65 0.3619936013168612 1102 66-67 0.5397761942037036 1102 68-69 0.49628373470133624 1102 70-71 0.29085040847429156 1102 72-73 0.30736173289848523 1102 74-75 0.05796113374281475 1102 76-77 0.19910411371031245 1102 78-79 0.23557791943875372 1102 80-81 0.10650495512717129 1102 82-83 0.114035397316961 1102 84-85 0.20537348105025188 1102 86-87 0.29568565339611297 1102 88-89 0.35327040942133436 1102 90-91 0.3389733996601123 1102 92-93 0.2810107650023852 1102 94-95 0.2224983390497144 1102 96-97 0.366718619684832 1102 98-99 0.18346984943864797 1102 100-101 0.14170324525223776 1103 1 0.5102161096085069 1103 2 0.48713182254187615 1103 3 0.5017557707725402 1103 4 0.5827550568823625 1103 5 0.5542660585538073 1103 6 0.483475647827305 1103 7 0.561426164299462 1103 8 0.5017458627506528 1103 9 0.5181904077844806 1103 10-11 0.5350636579024837 1103 12-13 0.6287456354648882 1103 14-15 0.6655726397738633 1103 16-17 0.6322350445297076 1103 18-19 0.46429413593410374 1103 20-21 0.6004801919967591 1103 22-23 0.5785180831458163 1103 24-25 0.621550779976765 1103 26-27 0.7608365144643869 1103 28-29 0.7077677595972816 1103 30-31 0.6332671143430062 1103 32-33 0.6021068183273712 1103 34-35 0.6155973360450702 1103 36-37 0.696409493923916 1103 38-39 0.8592044466388771 1103 40-41 0.8778919383188679 1103 42-43 0.5586772555272432 1103 44-45 0.7773801244494436 1103 46-47 0.7748771164677706 1103 48-49 0.9743290219615233 1103 50-51 0.9494750094126303 1103 52-53 1.036608291789598 1103 54-55 1.1325958850266886 1103 56-57 1.0137688338641269 1103 58-59 1.1814016680520112 1103 60-61 1.0453846970007774 1103 62-63 1.1848432130582012 1103 64-65 1.094593054702834 1103 66-67 0.7741369594633483 1103 68-69 0.6754896259858825 1103 70-71 0.7682114774083999 1103 72-73 0.7273966554615043 1103 74-75 0.7947285191057034 1103 76-77 0.7426282558299633 1103 78-79 0.9508907041335632 1103 80-81 0.8759780799373686 1103 82-83 0.7095334234329655 1103 84-85 0.8488292964161772 1103 86-87 0.9227582315922902 1103 88-89 0.8210809165576833 1103 90-91 0.658636321008423 1103 92-93 0.6413129210910498 1103 94-95 0.5339620499516258 1103 96-97 0.599029582332868 1103 98-99 0.5403784434258903 1103 100-101 0.7944287235395144 1104 1 -0.3834258352083708 1104 2 -0.2533381513484656 1104 3 -0.38245846957747176 1104 4 -0.28333061103098345 1104 5 -0.17128612548401634 1104 6 -0.4036812218360879 1104 7 -0.35271539240084593 1104 8 -0.22257140490864913 1104 9 -0.32704987165945454 1104 10-11 -0.3850266674097753 1104 12-13 -0.4453493472462853 1104 14-15 -0.4378674844234425 1104 16-17 -0.40132292597343167 1104 18-19 -0.4689180579830463 1104 20-21 -0.25449828517493245 1104 22-23 -0.29844190556352146 1104 24-25 -0.36211656883418897 1104 26-27 -0.4253183087661512 1104 28-29 -0.19520309637618283 1104 30-31 -0.2737472106869845 1104 32-33 -0.1760457467703631 1104 34-35 -0.2076896585213035 1104 36-37 -0.25046087513965887 1104 38-39 -0.22904620610263038 1104 40-41 -0.40567308884928366 1104 42-43 -0.3459307467308861 1104 44-45 0.054791739720066346 1104 46-47 0.3029026615951409 1104 48-49 0.3555441796779846 1104 50-51 0.17506600510806436 1104 52-53 0.20608115891258905 1104 54-55 0.5501175842716179 1104 56-57 0.362953789054302 1104 58-59 0.2133754172581277 1104 60-61 0.29016629321841236 1104 62-63 0.4355171245676175 1104 64-65 0.4221705016014283 1104 66-67 0.5051368888967005 1104 68-69 0.5173144795600848 1104 70-71 0.26237914376927307 1104 72-73 0.4373783081324518 1104 74-75 0.3414013015487214 1104 76-77 0.4074790073647847 1104 78-79 0.2763229248660437 1104 80-81 0.32368819213834144 1104 82-83 0.38203712818210533 1104 84-85 0.3330541217637162 1104 86-87 0.5022233363837714 1104 88-89 0.24919114166529965 1104 90-91 0.2209078614784019 1104 92-93 0.14046964962115283 1104 94-95 0.3029762338483124 1104 96-97 0.385794807087656 1104 98-99 0.3405972000415183 1104 100-101 0.33113396664161954 1105 1 0.04693943086791563 1105 2 0.008781307077953215 1105 3 -0.01643871237040173 1105 4 0.16447698499604257 1105 5 0.14309302985500238 1105 6 0.15773031486548206 1105 7 0.21029771626379556 1105 8 0.15054775742883209 1105 9 0.3302271868315714 1105 10-11 0.16430578662914286 1105 12-13 0.09597188233031062 1105 14-15 0.26447902038128035 1105 16-17 0.27431501109420964 1105 18-19 0.31558976145654327 1105 20-21 0.2547780465523459 1105 22-23 0.337684982003438 1105 24-25 0.256978475713737 1105 26-27 0.2362823210957643 1105 28-29 0.19456770387144928 1105 30-31 0.24013532275760952 1105 32-33 0.16969528308078452 1105 34-35 0.2490746277697795 1105 36-37 0.271702054525754 1105 38-39 0.2244106322058954 1105 40-41 0.0321549642313812 1105 42-43 0.29205006132273326 1105 44-45 0.3395283551543784 1105 46-47 0.4188517612156062 1105 48-49 0.13037527439952612 1105 50-51 0.1718628611819284 1105 52-53 0.3915539591063961 1105 54-55 0.4006162249542484 1105 56-57 0.3462987864971723 1105 58-59 0.6771247106822713 1105 60-61 0.5979271879453307 1105 62-63 0.547778571096643 1105 64-65 0.5344857824821574 1105 66-67 0.5235448725310548 1105 68-69 0.6582494476632306 1105 70-71 0.7569687914234464 1105 72-73 0.929639620075605 1105 74-75 0.754633785196539 1105 76-77 0.6341788270197029 1105 78-79 0.7001035768000392 1105 80-81 0.8251282610463093 1105 82-83 0.9613654100587681 1105 84-85 0.6813522833206136 1105 86-87 0.7177289755321041 1105 88-89 0.4856114264490188 1105 90-91 0.4411105478125492 1105 92-93 0.6352039770955074 1105 94-95 0.5900849254043976 1105 96-97 0.5170429565318173 1105 98-99 0.5490438098232211 1105 100-101 0.4674156279697179 1106 1 0.2833344602329717 1106 2 0.21133720151497926 1106 3 0.30135928626847175 1106 4 0.1374434861429208 1106 5 0.254365179679823 1106 6 0.2650325103132616 1106 7 0.23050037298246906 1106 8 0.1344095778104588 1106 9 0.15344448827083568 1106 10-11 0.2668881474286806 1106 12-13 0.2971193881907226 1106 14-15 0.2600648092049127 1106 16-17 0.21766754304289293 1106 18-19 0.2546100019223516 1106 20-21 0.3526311204313046 1106 22-23 0.44006569432667675 1106 24-25 0.29824079517533875 1106 26-27 0.43836245115902983 1106 28-29 0.5072457216669264 1106 30-31 0.5008478980607123 1106 32-33 0.5126268738352024 1106 34-35 0.4543338720921142 1106 36-37 0.6586245206866224 1106 38-39 0.6834098256119887 1106 40-41 0.6974121920689527 1106 42-43 0.5858298359805616 1106 44-45 0.7046318260287734 1106 46-47 0.5858828654930761 1106 48-49 0.49637356038747527 1106 50-51 0.4353039924298727 1106 52-53 0.41246238549209835 1106 54-55 0.47546246799106484 1106 56-57 0.5167358595695362 1106 58-59 0.5548438143547898 1106 60-61 0.65114033038477 1106 62-63 0.4110640549269675 1106 64-65 0.3767116035695466 1106 66-67 0.36440326488857266 1106 68-69 0.39372130509775616 1106 70-71 0.2591617186031385 1106 72-73 0.4114282420603246 1106 74-75 0.29381660474636107 1106 76-77 0.3182114184546876 1106 78-79 0.5057112948954696 1106 80-81 0.4356820381743347 1106 82-83 0.38241057931745814 1106 84-85 0.38641933143897944 1106 86-87 0.4235049440749421 1106 88-89 0.3587304242560876 1106 90-91 0.43870967064167843 1106 92-93 0.48979967190054907 1106 94-95 0.6197745127812055 1106 96-97 0.5733406905070595 1106 98-99 0.612386637438977 1106 100-101 0.29240995660631697 1107 1 0.08917780511696805 1107 2 0.1816162350118944 1107 3 0.16312073285692463 1107 4 0.17924867280044054 1107 5 0.1372461609583695 1107 6 0.3287605010258261 1107 7 0.16252150424372047 1107 8 0.06816623996239457 1107 9 0.30306953151252713 1107 10-11 0.23741637032713214 1107 12-13 0.10794065782538098 1107 14-15 0.2519910725951746 1107 16-17 0.1672697194924453 1107 18-19 0.1863467641018488 1107 20-21 0.27517719115419226 1107 22-23 0.17459689577138704 1107 24-25 0.23001860031509125 1107 26-27 0.0894709042498576 1107 28-29 0.16803738173740612 1107 30-31 0.05797383545260004 1107 32-33 0.21628790588975733 1107 34-35 0.2273868233741183 1107 36-37 0.1401258721726677 1107 38-39 0.0757512092288124 1107 40-41 0.22589893812443052 1107 42-43 0.4462720445608852 1107 44-45 0.1182188048564683 1107 46-47 0.08976142523697206 1107 48-49 0.22008701016554966 1107 50-51 0.03633417073222489 1107 52-53 0.14049707265679245 1107 54-55 0.23706150172252194 1107 56-57 0.13887091436188825 1107 58-59 -0.08025495259742144 1107 60-61 -0.05924295223118747 1107 62-63 -0.06882196290914067 1107 64-65 0.06733270082376919 1107 66-67 -2.1653071748062303E-4 1107 68-69 -0.03965717549185399 1107 70-71 -0.09583350282363057 1107 72-73 -0.30463069563059264 1107 74-75 -0.3277291904023656 1107 76-77 -0.04090275719745051 1107 78-79 -0.14861023565514841 1107 80-81 -0.01588140544729555 1107 82-83 -0.021560620251001694 1107 84-85 -0.05219798986290414 1107 86-87 0.10315877602160839 1107 88-89 0.09324696750071126 1107 90-91 0.046286201104344116 1107 92-93 -0.10028083093435214 1107 94-95 -0.2840559680663901 1107 96-97 -0.23918093033806898 1107 98-99 -0.15612813509507717 1107 100-101 -0.06966810710408211 1108 1 0.22322326195992304 1108 2 0.15598428155826838 1108 3 0.0403468208389981 1108 4 0.16958333715327 1108 5 0.12055506418707296 1108 6 0.3037744009129426 1108 7 0.2462353294364945 1108 8 0.17971915219935397 1108 9 0.05423066349686678 1108 10-11 0.14387434212299866 1108 12-13 0.1208970374523588 1108 14-15 0.1012236303428935 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2316 74-75 -0.7973759449088718 2316 76-77 -1.0298284705593517 2316 78-79 -0.4399838783140595 2316 80-81 -0.29283961416122395 2316 82-83 -0.37238782365681544 2316 84-85 -0.29275385977371826 2316 86-87 -0.21835929042411095 2316 88-89 -0.28343371042885934 2316 90-91 -0.19941611344849974 2316 92-93 -0.43668586421110334 2316 94-95 -0.4404230092648973 2316 96-97 -0.2962027286297726 2316 98-99 -0.17046273483101615 2316 100-101 -0.2370420730998255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 19.0 7 333.0 8 773.0 9 574.0 10 511.0 11 575.0 12 727.0 13 639.0 14 631.0 15 631.0 16 763.0 17 945.0 18 1315.0 19 1798.0 20 2364.0 21 2896.0 22 3263.0 23 3223.0 24 2837.0 25 2507.0 26 2327.0 27 2342.0 28 2643.0 29 2922.0 30 3428.0 31 4262.0 32 5569.0 33 7391.0 34 10927.0 35 18716.0 36 36139.0 37 84652.0 38 148152.0 39 56486.0 40 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71209812032804 19.914120521566637 14.027868495536685 23.345912862568643 2 15.555555555555555 21.79161525993662 39.561167960906694 23.091661223601132 3 17.31160945400005 26.19856303069889 32.257541669690596 24.23228584561047 4 12.226092846602317 18.733096257590052 39.28804702808622 29.75276386772141 5 12.87998645281467 37.802453008200885 36.124537339429565 13.19302319955488 6 29.33546217674239 38.67237583762731 18.497230084427994 13.494931901202312 7 24.945448387643033 34.17471030795655 23.583714347920747 17.296126956479668 8 24.50443912233592 34.57556184532017 22.001112804509276 18.918886227834626 9 25.511769117255728 15.335413793937636 21.77951955874882 37.37329753005782 10-11 23.15274451459951 26.482932965624016 30.68522147228875 19.679101047487723 12-13 24.38783656288555 25.254977381038778 29.483513559281032 20.87367249679464 14-15 21.864068510051528 26.831168202820717 27.49812516631589 23.80663812081186 16-17 20.888066381208116 29.137213634274378 28.419333768778575 21.555386215738924 18-19 20.75332026997605 28.17185572247623 30.54914483392602 20.525679173621693 20-21 21.79754215351864 27.63480658973801 30.18845102450589 20.379200232237462 22-23 21.466871648730738 27.65008061794593 29.84315485298262 21.039892880340712 24-25 21.245278134937386 27.69338328057585 30.184979782011013 20.87635880247575 26-27 21.311657836804798 27.67968164114474 30.02830394077945 20.980356581271018 28-29 21.326051721218278 27.71971841207635 30.04765706267992 20.90657280402545 30-31 21.486682632992235 27.720565111159495 29.964559595519752 20.828192660328522 32-33 21.17219440210949 27.867648837603117 29.969276918982995 20.990879841304398 34-35 21.56228076541597 27.95715702639282 29.650192321648888 20.830369886542325 36-37 21.564941819677287 27.738587705929312 29.556087766407817 21.140382707985584 38-39 21.288676004547984 27.937320076444834 29.730265863512106 21.043738055495076 40-41 21.475675544911336 27.951472046834553 29.577860028545857 20.99499237970825 42-43 21.520913467353704 27.951834917870187 29.425333236567724 21.101918378208385 44-45 21.60292232140697 28.048963398408205 29.136125021167476 21.211989259017344 46-47 21.656627234680794 28.07726733918765 28.855383796598687 21.410721629532866 48-49 21.584899726637154 28.189636403222295 28.797687301932896 21.42777656820766 50-51 21.567240002902967 28.2588238140165 28.696567240002903 21.47736894307763 52-53 21.518131456080507 28.257493286885843 28.678181774197448 21.546193482836202 54-55 21.543532428574885 28.31990710501488 28.686285893993276 21.450274572416962 56-57 21.655901492609527 28.299949198055014 28.3971986356049 21.646950673730554 58-59 21.479909040327065 28.149478675278804 28.773133028521663 21.59747925587246 60-61 21.60897017200087 28.08162179161526 28.741684205433387 21.56772383095048 62-63 21.421849674625637 28.09287079371991 28.801678883324865 21.683600648329584 64-65 21.3440743159881 28.04690712920628 28.771197716331613 21.837820838474006 66-67 21.368144761351815 28.164477344751678 28.597624404286716 21.869753489609792 68-69 21.429953794421465 28.142705082613638 28.467232745482256 21.960108377482644 70-71 21.407939618259668 27.908048479570365 28.545612889179182 22.138399012990785 72-73 21.381208118634635 27.959213295594747 28.41606792945787 22.243510656312747 74-75 21.410721629532866 27.969373684592497 28.37421680334809 22.24568788252655 76-77 21.30754529840095 27.850472941916443 28.550572126666186 22.291409633016425 78-79 21.29206280088057 27.889058228705515 28.429857028811963 22.389021941601957 80-81 21.350968865665145 27.850231027892686 28.347001475675544 22.451798630766625 82-83 21.4599511333672 27.79326027529816 28.37216053414616 22.374628057188474 84-85 21.366814234221156 27.72576626267025 28.440622202869097 22.466797300239495 86-87 21.492125698526742 27.64835377506834 28.242857488448603 22.61666303795631 88-89 21.43733217214602 27.568280233205115 28.266686019788565 22.727701574860294 90-91 21.60727677383458 27.46679730023949 28.403609357234437 22.522316568691487 92-93 21.565183733701044 27.377168154437914 28.363935457338464 22.69371265452258 94-95 21.39717444420253 27.23976098894453 28.48247332897888 22.88059123787406 96-97 21.3879817112998 27.217988726806492 28.4263492754675 22.967680286426205 98-99 21.522727822531873 27.116868664876502 28.394295667319835 22.96610784527179 100-101 21.703800469313205 27.023731765730457 28.348936787865593 22.923530977090742 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 363.0 1 233.5 2 128.5 3 148.5 4 127.0 5 124.0 6 165.5 7 294.0 8 474.5 9 562.5 10 482.0 11 391.5 12 398.0 13 438.0 14 486.0 15 549.5 16 580.5 17 584.0 18 645.0 19 705.5 20 801.0 21 973.5 22 1199.0 23 1488.0 24 1814.0 25 2203.0 26 2808.5 27 3533.5 28 4266.0 29 5115.5 30 6124.5 31 7018.0 32 7804.0 33 8715.0 34 9697.5 35 10550.5 36 11470.0 37 12359.0 38 12970.0 39 13431.0 40 14085.5 41 14786.5 42 15182.5 43 15546.0 44 15831.5 45 15943.0 46 15984.0 47 15815.5 48 15541.0 49 15374.5 50 14895.5 51 14011.5 52 13165.0 53 12480.5 54 11546.5 55 10374.0 56 9083.0 57 7760.5 58 6753.0 59 5816.5 60 4803.0 61 3942.0 62 3129.0 63 2385.0 64 1723.0 65 1249.5 66 953.0 67 682.0 68 526.0 69 443.5 70 351.5 71 262.5 72 206.0 73 161.0 74 123.0 75 91.0 76 73.0 77 58.5 78 39.5 79 30.5 80 25.5 81 18.0 82 16.5 83 16.0 84 12.5 85 8.0 86 3.5 87 2.5 88 2.5 89 3.0 90 3.0 91 3.0 92 1.5 93 1.5 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.2095701187797855E-4 24-25 1.2095701187797855E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 413370.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.9782342468925 #Duplication Level Percentage of deduplicated Percentage of total 1 80.5925311538088 60.42685679400257 2 13.362417062362352 20.03780873212955 3 3.591662391894334 8.078895124656228 4 1.2486983901590099 3.745008016042392 5 0.5292548122013515 1.9841295642764014 6 0.2685965263685781 1.208333596317893 7 0.13061538083219273 0.6855317434198255 8 0.0782116091477547 0.46913346812054013 9 0.04993478586755983 0.3369619864661686 >10 0.14060395648864724 1.5486630630649443 >50 0.004549349233536352 0.23121854387280097 >100 0.0019497210905613323 0.2960279554045339 >500 3.249535150935554E-4 0.21343250118055282 >1k 6.499070301871108E-4 0.7379989110455417 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.38996540629460286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1417 0.3427921716621912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.21191668481021844 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.2095701187797857E-4 0.0 20-21 0.0 0.0 0.0 3.628710356339357E-4 0.0 22-23 0.0 0.0 0.0 8.4669908314585E-4 0.0 24-25 0.0 0.0 0.0 0.002298183225681593 0.0 26-27 0.0 0.0 0.0 0.004596366451363186 0.0 28-29 0.0 0.0 0.0 0.013063357282821686 0.0 30-31 0.0 0.0 0.0 0.030844038028884534 0.0 32-33 0.0 0.0 0.0 0.05733362363016184 0.0 34-35 0.0 0.0 0.0 0.10112006192999008 0.0 36-37 0.0 0.0 0.0 0.16849311754602414 0.0 38-39 0.0 0.0 0.0 0.26259767278709145 0.0 40-41 0.0 0.0 0.0 0.36383869172895955 0.0 42-43 0.0 0.0 0.0 0.44983912717420227 0.0 44-45 0.0 0.0 0.0 0.559184265911895 0.0 46-47 0.0 0.0 0.0 0.7002201417616178 0.0 48-49 0.0 0.0 0.0 0.8382320923143914 0.0 50-51 0.0 0.0 0.0 0.9854367757698914 0.0 52-53 0.0 0.0 0.0 1.1157074775624745 0.0 54-55 0.0 0.0 0.0 1.2764593463483078 0.0 56-57 0.0 0.0 0.0 1.4697486513293176 0.0 58-59 0.0 0.0 0.0 1.6949706074461137 0.0 60-61 0.0 0.0 0.0 1.9310786946319278 0.0 62-63 0.0 0.0 0.0 2.1509785422260927 0.0 64-65 0.0 0.0 0.0 2.3943440501245856 0.0 66-67 0.0 0.0 0.0 2.6812540822991506 0.0 68-69 0.0 0.0 0.0 3.030336018578997 0.0 70-71 0.0 0.0 0.0 3.391150785011007 0.0 72-73 0.0 0.0 0.0 3.7604325422744758 0.0 74-75 0.0 0.0 0.0 4.150276991557201 0.0 76-77 0.0 0.0 0.0 4.568183467595617 0.0 78-79 0.0 0.0 0.0 5.041972083121658 0.0 80-81 0.0 0.0 0.0 5.559305222923773 0.0 82-83 0.0 0.0 0.0 6.102886034303409 0.0 84-85 0.0 0.0 0.0 6.682391078210804 0.0 86-87 0.0 0.0 0.0 7.306166388465539 0.0 88-89 0.0 0.0 0.0 8.005418874132134 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 715 0.0 53.14631 1 GTATCAA 1340 0.0 42.53688 1 TCAACGC 1585 0.0 35.362415 4 ATCAACG 1610 0.0 34.81331 3 CAACGCA 1615 0.0 34.70553 5 AACGCAG 1645 0.0 33.78385 6 TATCAAC 1725 0.0 32.767784 2 ACGCAGA 1790 0.0 30.516449 7 CGCAGAG 1775 0.0 30.506733 8 GCAGAGT 1935 0.0 28.229687 9 GCGTGCG 100 0.001651959 23.749758 9 AGAGTAC 1755 0.0 23.276117 10-11 CAGAGTA 1895 0.0 22.809793 10-11 GAGTACT 1420 0.0 22.244492 12-13 GTCATAC 110 0.0028744538 21.590689 1 GTACATG 950 0.0 20.999784 1 TACATGG 940 0.0 20.717875 2 AGTACTT 1535 0.0 20.577967 12-13 CGCAGAA 140 4.8448428E-4 20.356936 9 ACATGGG 940 0.0 20.21256 3 >>END_MODULE