FastQCFastQC Report
Fri 10 Feb 2017
SRR2031827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413370
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT28290.6843747732056028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.43326801654691927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.38996540629460286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9490.2295764085444033No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT6770.16377579408278298RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT5470.13232697099450855RNA PCR Primer, Index 31 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.061.8949471
GTATCAA13950.038.5313071
TCAACGC18200.030.0127034
ATCAACG18100.029.9160983
CAACGCA18450.029.3485855
TATCAAC18800.028.80222
AACGCAG18800.028.80226
CGCAGAG20400.026.0775348
ACGCAGA20800.026.0327597
GCAGAGT22300.023.8556829
TAAGGGT1252.2530353E-422.7992174
TACATGG9450.022.6182692
GTACATG9550.022.4139651
GAGTACT17600.022.12992112-13
CAGAGTA22250.022.09474610-11
GTAAGGG1302.937782E-421.9223233
AGAGTAC21100.021.72318510-11
GTCTAAT1353.754134E-421.1410731
GTACAAG2509.291398E-920.929661
CCGTCTT7300.020.8237246-47
Produced by FastQC (version 0.11.5)