##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031827_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 413370 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.924684906984055 34.0 33.0 34.0 31.0 34.0 2 33.06806734886421 34.0 33.0 34.0 31.0 34.0 3 33.10992089411423 34.0 33.0 34.0 31.0 34.0 4 36.45643612260203 37.0 37.0 37.0 35.0 37.0 5 36.45225584827153 37.0 37.0 37.0 35.0 37.0 6 36.51257711009507 37.0 37.0 37.0 35.0 37.0 7 36.4665529670755 37.0 37.0 37.0 35.0 37.0 8 36.50976123085855 37.0 37.0 37.0 35.0 37.0 9 38.324747804630235 39.0 39.0 39.0 37.0 39.0 10-11 38.345588697776805 39.0 39.0 39.0 37.0 39.0 12-13 38.364656361129256 39.0 39.0 39.0 37.0 39.0 14-15 39.95074388562305 41.0 40.0 41.0 38.0 41.0 16-17 39.924776834313086 41.0 40.0 41.0 38.0 41.0 18-19 39.961379635677474 41.0 40.0 41.0 38.0 41.0 20-21 39.962188838086945 41.0 40.0 41.0 38.0 41.0 22-23 39.92675811016765 41.0 40.0 41.0 38.0 41.0 24-25 39.85778600285458 41.0 40.0 41.0 38.0 41.0 26-27 39.73700074993347 41.0 40.0 41.0 37.0 41.0 28-29 39.68242252703389 41.0 40.0 41.0 37.0 41.0 30-31 39.541222149648014 41.0 40.0 41.0 36.5 41.0 32-33 39.08589762198515 40.5 39.0 41.0 35.0 41.0 34-35 39.115766746498295 41.0 39.0 41.0 35.0 41.0 36-37 39.04767399666159 41.0 39.0 41.0 35.0 41.0 38-39 38.94162856520792 41.0 39.0 41.0 35.0 41.0 40-41 38.85235503302127 41.0 39.0 41.0 35.0 41.0 42-43 38.6998451750248 40.5 39.0 41.0 35.0 41.0 44-45 38.46342380917822 40.0 38.0 41.0 35.0 41.0 46-47 38.24587657546508 40.0 38.0 41.0 34.5 41.0 48-49 38.08762246897453 40.0 38.0 41.0 34.0 41.0 50-51 38.07902000628977 40.0 38.0 41.0 34.0 41.0 52-53 38.140856859472144 41.0 38.5 41.0 34.0 41.0 54-55 38.0631843142947 41.0 38.0 41.0 34.0 41.0 56-57 37.90235019474079 40.5 38.0 41.0 34.0 41.0 58-59 37.72962358177904 40.0 37.0 41.0 34.0 41.0 60-61 37.5112393255437 40.0 37.0 41.0 33.0 41.0 62-63 37.26159856786897 40.0 36.0 41.0 33.0 41.0 64-65 36.96551515591359 39.0 36.0 41.0 32.5 41.0 66-67 36.659189104192365 39.0 35.0 41.0 32.0 41.0 68-69 36.22383216005032 38.5 35.0 41.0 31.0 41.0 70-71 35.80920240946368 37.0 35.0 40.0 31.0 41.0 72-73 35.37309311270774 37.0 35.0 39.5 30.5 41.0 74-75 34.9645692720807 36.5 35.0 39.0 30.0 41.0 76-77 33.53020780414641 35.0 33.5 37.0 27.5 39.0 78-79 34.170534871906526 35.0 35.0 37.0 29.0 39.0 80-81 33.995355250743884 35.0 35.0 37.0 29.0 39.0 82-83 33.685180346904716 35.0 35.0 36.5 29.0 39.0 84-85 33.425330817427486 35.0 35.0 36.0 29.0 37.0 86-87 33.183797808258944 35.0 35.0 36.0 29.0 37.0 88-89 33.0059643902557 35.0 34.0 35.5 29.0 37.0 90-91 32.81581150059269 35.0 34.0 35.0 28.5 36.0 92-93 32.67896799477465 35.0 34.0 35.0 27.0 36.0 94-95 32.60553378329342 35.0 34.0 35.0 27.0 36.0 96-97 32.52771608970172 35.0 34.0 35.0 27.0 36.0 98-99 32.471207392892566 35.0 34.0 35.0 27.0 35.5 100-101 31.350222560901855 34.5 32.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3200624298206165 1101 2 0.3496145415423655 1101 3 0.37060703278410756 1101 4 0.1564081200704095 1101 5 0.043436467904683695 1101 6 0.04320381973292342 1101 7 0.1987452895269044 1101 8 0.10589784543378045 1101 9 0.22373308512965195 1101 10-11 0.1224350735799149 1101 12-13 0.10685969098116743 1101 14-15 0.1002653953868986 1101 16-17 0.13008381725767748 1101 18-19 -0.015389250021918599 1101 20-21 0.11226594277518842 1101 22-23 0.15759614395423682 1101 24-25 0.0797601948597162 1101 26-27 0.16826303908855778 1101 28-29 0.3176503943813316 1101 30-31 0.1581084842496452 1101 32-33 0.062171524399865064 1101 34-35 0.060013025128832 1101 36-37 0.29240158324306975 1101 38-39 0.20026109956064886 1101 40-41 0.06061951147202649 1101 42-43 -0.012243413480042875 1101 44-45 -0.0902186120078241 1101 46-47 -0.1705368706690038 1101 48-49 0.17417504492628666 1101 50-51 0.4214343489815846 1101 52-53 0.23227869965924697 1101 54-55 -0.023304547978789003 1101 56-57 0.18158309362161873 1101 58-59 0.07318929523840012 1101 60-61 0.07745833713756411 1101 62-63 -0.08356634882938607 1101 64-65 0.06985382217551717 1101 66-67 0.26411287077711876 1101 68-69 0.07751870588585064 1101 70-71 -0.0955695837841688 1101 72-73 0.23456994421854915 1101 74-75 0.14838173410971933 1101 76-77 0.06950453582249594 1101 78-79 0.010101640921128308 1101 80-81 0.22345811315441466 1101 82-83 0.22568605694858945 1101 84-85 0.31815386236371523 1101 86-87 0.1707737514736607 1101 88-89 0.06174142150371864 1101 90-91 0.18571915563156693 1101 92-93 0.2157342576320218 1101 94-95 0.0993889973748523 1101 96-97 0.37123587517702816 1101 98-99 0.17927423368700346 1101 100-101 -0.04573310472334313 1102 1 0.44041679455700233 1102 2 0.26702362338532737 1102 3 0.3494814427302586 1102 4 0.22124755893750603 1102 5 0.23830383044706593 1102 6 0.21215867645787512 1102 7 0.25793350839533247 1102 8 0.11129974690310007 1102 9 0.17980316025763443 1102 10-11 0.19415570385715597 1102 12-13 0.09260698171981119 1102 14-15 0.11234903344287517 1102 16-17 0.26139988666795233 1102 18-19 0.21408352439468104 1102 20-21 0.20631720266482034 1102 22-23 0.16604804209930535 1102 24-25 0.3083520438059324 1102 26-27 0.23616078524905504 1102 28-29 0.321115921939338 1102 30-31 0.316683379535867 1102 32-33 0.3461536399506926 1102 34-35 0.10506488338293707 1102 36-37 0.33699635752669366 1102 38-39 0.5188320057503972 1102 40-41 0.4266440444153119 1102 42-43 0.2875288755964789 1102 44-45 0.227188807711606 1102 46-47 0.2100894174767305 1102 48-49 0.5472469816326253 1102 50-51 0.5385891259246094 1102 52-53 0.43326267678842356 1102 54-55 0.43918963458885685 1102 56-57 0.23393167416812588 1102 58-59 0.07677117144344692 1102 60-61 0.31638028381664185 1102 62-63 0.18500788160741877 1102 64-65 0.16274159559483792 1102 66-67 0.2892192466829684 1102 68-69 0.35868352205498155 1102 70-71 0.1706444312414277 1102 72-73 0.3314135716369293 1102 74-75 0.26665013381718694 1102 76-77 0.2386505367532905 1102 78-79 0.5173983632135304 1102 80-81 0.38004677082344784 1102 82-83 0.4298363794005553 1102 84-85 0.16566233919371598 1102 86-87 0.47533295631376404 1102 88-89 0.47691494720014305 1102 90-91 0.6042584814742611 1102 92-93 0.4368881037858685 1102 94-95 0.4759597041097763 1102 96-97 0.4136615782552795 1102 98-99 0.3827513961097111 1102 100-101 0.20940285698132755 1103 1 0.405509415267602 1103 2 0.3302638298839611 1103 3 0.2972342517190185 1103 4 0.19854789297941267 1103 5 0.18036274329249835 1103 6 0.10127194703181885 1103 7 0.14683420684659154 1103 8 0.18781052734646408 1103 9 0.23147336979301514 1103 10-11 0.17364249401810383 1103 12-13 0.12512292462901797 1103 14-15 0.3647784291307019 1103 16-17 0.12271935220091024 1103 18-19 0.11496418033151201 1103 20-21 0.26308155128310773 1103 22-23 0.07475590726784276 1103 24-25 0.23938757371785613 1103 26-27 0.32254098567419476 1103 28-29 0.15961425172979915 1103 30-31 0.11433749432481477 1103 32-33 0.2455235154441695 1103 34-35 0.07175938687519334 1103 36-37 0.08594868063632077 1103 38-39 0.16650130426238974 1103 40-41 0.14661823208613356 1103 42-43 0.2520217392466435 1103 44-45 0.4666740795002582 1103 46-47 -0.09769983244735414 1103 48-49 -0.18888268736220937 1103 50-51 -0.08402702955671515 1103 52-53 -0.1254375965185872 1103 54-55 0.025304119860642516 1103 56-57 -0.01656179683096326 1103 58-59 0.1497365525548915 1103 60-61 -0.2548541528672388 1103 62-63 -0.06035349100114473 1103 64-65 0.06132191748976368 1103 66-67 0.13183236541969023 1103 68-69 0.5326282533030948 1103 70-71 0.34868117585121183 1103 72-73 0.20322498979666648 1103 74-75 0.42605645024598005 1103 76-77 0.33931102870591445 1103 78-79 0.302913091424891 1103 80-81 0.4581214747408566 1103 82-83 0.454016458355909 1103 84-85 0.3029611548633113 1103 86-87 0.34592967662958785 1103 88-89 0.43301121200426707 1103 90-91 0.26544888536129463 1103 92-93 0.36188810378586567 1103 94-95 0.4372108428890016 1103 96-97 0.4286403210430123 1103 98-99 0.39834507968092225 1103 100-101 0.16079975950182757 1104 1 0.05535064030464554 1104 2 0.06630927480668447 1104 3 0.07739090968246387 1104 4 -0.03495715253607301 1104 5 -0.0024331073884695797 1104 6 0.05461327791883974 1104 7 0.14176752136214787 1104 8 0.028372237882066997 1104 9 0.28091942158893346 1104 10-11 0.07369189067324555 1104 12-13 0.17099830392476179 1104 14-15 0.2073265908694637 1104 16-17 0.016170767062241964 1104 18-19 -0.046118312082569446 1104 20-21 0.07886025426807208 1104 22-23 0.1700561683644466 1104 24-25 0.13510417805066055 1104 26-27 0.050584172464127164 1104 28-29 0.013040262597066032 1104 30-31 -0.021051680751050128 1104 32-33 0.18944067019683075 1104 34-35 0.0596113380430765 1104 36-37 0.06182547126506677 1104 38-39 -0.04836003226996155 1104 40-41 0.0195276950739256 1104 42-43 0.09101334181527676 1104 44-45 0.2152451048336772 1104 46-47 0.26420828977408206 1104 48-49 0.2284414252621616 1104 50-51 0.29027965310505977 1104 52-53 0.11902997810564386 1104 54-55 -0.018422504936474127 1104 56-57 0.07281086564875494 1104 58-59 0.009711148560818117 1104 60-61 0.1352665161046076 1104 62-63 -0.12591343737049243 1104 64-65 0.06246509722302562 1104 66-67 -0.13250677790117749 1104 68-69 -0.21233328787254635 1104 70-71 -0.4293020292860348 1104 72-73 -0.5527908171329798 1104 74-75 -0.3581225067789333 1104 76-77 -0.2489290390380745 1104 78-79 -0.24905218978320676 1104 80-81 -0.2697170687634838 1104 82-83 -0.11192807625702983 1104 84-85 -0.05056012098171436 1104 86-87 0.061263809718283824 1104 88-89 -0.013965007034606458 1104 90-91 0.08780792847752394 1104 92-93 0.06254084530022652 1104 94-95 0.04886139683721069 1104 96-97 -0.08109082001971757 1104 98-99 -0.2179981565056508 1104 100-101 -0.18197068718507126 1105 1 0.3985715495668458 1105 2 0.3404337936621715 1105 3 0.34053322079118686 1105 4 0.20969305876375444 1105 5 0.14919807346803537 1105 6 0.17627891276043073 1105 7 0.2576728805718602 1105 8 0.18896683829658656 1105 9 0.14441569356007733 1105 10-11 0.32083530538617566 1105 12-13 0.1614561650859656 1105 14-15 0.3081818841320967 1105 16-17 0.32473244777070676 1105 18-19 0.28982486575921484 1105 20-21 0.3526886841892036 1105 22-23 0.2590342577833056 1105 24-25 0.37213346393797764 1105 26-27 0.43696004390768906 1105 28-29 0.43687811352975103 1105 30-31 0.5105760008725966 1105 32-33 0.8313412919835983 1105 34-35 0.6434785286974289 1105 36-37 0.6351545875741849 1105 38-39 0.6678387241565176 1105 40-41 0.716867605170556 1105 42-43 0.6797592705924274 1105 44-45 1.0052642160285288 1105 46-47 1.0703205074802042 1105 48-49 0.855830324618843 1105 50-51 0.8665023658180431 1105 52-53 0.9995345405669482 1105 54-55 1.0144097203064533 1105 56-57 0.9710740348770344 1105 58-59 1.2267914424963777 1105 60-61 0.9643155322818302 1105 62-63 0.999426391974616 1105 64-65 0.7255529010506478 1105 66-67 0.6932213317055655 1105 68-69 0.6934599612997445 1105 70-71 0.4589277626441941 1105 72-73 0.5010098880970304 1105 74-75 0.5745239899506274 1105 76-77 0.386629223189054 1105 78-79 0.5782404806698054 1105 80-81 0.7055998809821418 1105 82-83 0.6462009108496432 1105 84-85 0.6805036458078675 1105 86-87 0.7074509917591456 1105 88-89 0.7142455391148914 1105 90-91 0.8828841040851714 1105 92-93 0.7255478976003005 1105 94-95 0.7283922304621413 1105 96-97 0.838615244420005 1105 98-99 0.6844136224504993 1105 100-101 0.9046420554059758 1106 1 0.42670151200982076 1106 2 0.3222350186766647 1106 3 0.30065723460410254 1106 4 0.21738090692245748 1106 5 0.20473332414229617 1106 6 0.18779808196870817 1106 7 0.21572999750276978 1106 8 0.12376506378999608 1106 9 0.19678758376248595 1106 10-11 0.18869568902240275 1106 12-13 0.13908578724714005 1106 14-15 0.2231839006168599 1106 16-17 0.2035889748798141 1106 18-19 0.20766886545673913 1106 20-21 0.2003180542897809 1106 22-23 0.2703549292727345 1106 24-25 0.24226869444739663 1106 26-27 0.31770387472461437 1106 28-29 0.17082815511974303 1106 30-31 0.17408770908725302 1106 32-33 0.49995092573954736 1106 34-35 0.3713728144837276 1106 36-37 0.46927981127796414 1106 38-39 0.2804972072558485 1106 40-41 0.2027868701618658 1106 42-43 0.1444455811814933 1106 44-45 0.31045918489905233 1106 46-47 0.1781740854803573 1106 48-49 0.4578297952915875 1106 50-51 0.4332795190456764 1106 52-53 0.6273576192350632 1106 54-55 0.40857577782667676 1106 56-57 0.2821892769812351 1106 58-59 0.4189561388893921 1106 60-61 0.0697146702345961 1106 62-63 0.3202617207876628 1106 64-65 0.49607378427097615 1106 66-67 0.3317695887065426 1106 68-69 0.22587810462139402 1106 70-71 0.39048385872968794 1106 72-73 0.3996500211850247 1106 74-75 0.42111361009531123 1106 76-77 0.10608893130288521 1106 78-79 0.16181747257866164 1106 80-81 0.2153672290526245 1106 82-83 0.3531111520716337 1106 84-85 0.19001263176809857 1106 86-87 0.1831397473359857 1106 88-89 0.1809924450710767 1106 90-91 0.021904974702472657 1106 92-93 0.05516438740445295 1106 94-95 0.13717780251366207 1106 96-97 0.1433565042188505 1106 98-99 0.2671021007606811 1106 100-101 0.22597520389487258 1107 1 0.30751861871565467 1107 2 0.20455898835177777 1107 3 0.127450079057148 1107 4 0.16620619876722031 1107 5 0.10371487240002608 1107 6 -0.029313314488696562 1107 7 0.034824355268391116 1107 8 0.022887330768590175 1107 9 0.05273851983513822 1107 10-11 -0.012247712735408811 1107 12-13 0.032206987108764906 1107 14-15 0.00971653270189421 1107 16-17 0.03417829315625198 1107 18-19 0.02547036997439278 1107 20-21 -0.02481137761701291 1107 22-23 0.029192358107167138 1107 24-25 0.07222184683990207 1107 26-27 0.16021030967445427 1107 28-29 -0.016087349151653996 1107 30-31 -0.0247771598977522 1107 32-33 0.2462526618567651 1107 34-35 0.1051154047635876 1107 36-37 0.09245266663671003 1107 38-39 0.08621742325908599 1107 40-41 0.02639009027525674 1107 42-43 -0.1359716498258834 1107 44-45 -0.24306083527063294 1107 46-47 -0.6124188680296996 1107 48-49 -0.7612399366978764 1107 50-51 -0.6056292072739993 1107 52-53 -0.6106505072710675 1107 54-55 -0.37280009946659476 1107 56-57 -0.40997679456250324 1107 58-59 -0.5913808238142835 1107 60-61 -0.6156617230273227 1107 62-63 -0.7419887233558029 1107 64-65 -0.8692114936191828 1107 66-67 -0.44977046042774305 1107 68-69 -0.62325676840927 1107 70-71 -0.47892916830589627 1107 72-73 -0.42102155891031146 1107 74-75 -0.5830757034904934 1107 76-77 -0.8989124465679268 1107 78-79 -0.7290786125092623 1107 80-81 -0.6122200898453158 1107 82-83 -0.4664903794171096 1107 84-85 -0.6586209308194881 1107 86-87 -0.6226684178677857 1107 88-89 -0.4544669136033477 1107 90-91 -0.4881417556394183 1107 92-93 -0.5370327595234841 1107 94-95 -0.42410226878382673 1107 96-97 -0.34283547129609104 1107 98-99 -0.39132372581521935 1107 100-101 -0.650989727827227 1108 1 0.26962106790384155 1108 2 0.18661549771103836 1108 3 0.2485223079251071 1108 4 0.08385708976402384 1108 5 0.004427557521246683 1108 6 0.029824387059669277 1108 7 0.19176110095381205 1108 8 0.12517365159483518 1108 9 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-0.7830400875585397 2316 52-53 -1.3195463119756212 2316 54-55 -0.897420852302524 2316 56-57 -0.8395701984910531 2316 58-59 -0.6849329484067468 2316 60-61 -0.6700429371571417 2316 62-63 -0.574823579066738 2316 64-65 -1.4519587789369979 2316 66-67 -0.895705846949987 2316 68-69 -1.8398183506041903 2316 70-71 -0.9590372166986398 2316 72-73 -0.8656838066402202 2316 74-75 -0.7909341358457311 2316 76-77 -0.406948714183045 2316 78-79 -0.44795744202991017 2316 80-81 -0.5394288846072541 2316 82-83 -0.5406692385769674 2316 84-85 -0.7308732413306274 2316 86-87 -0.6861838976188182 2316 88-89 -0.7080101464328195 2316 90-91 -1.221584215154948 2316 92-93 -0.8323066527684375 2316 94-95 -0.4528792472010821 2316 96-97 -0.44261182998442195 2316 98-99 -0.23428980239216202 2316 100-101 -0.49190800444189264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 17.0 15 79.0 16 200.0 17 516.0 18 867.0 19 1307.0 20 1900.0 21 2523.0 22 3174.0 23 3282.0 24 2904.0 25 2663.0 26 2611.0 27 2933.0 28 3553.0 29 3838.0 30 3511.0 31 4050.0 32 5034.0 33 6710.0 34 9590.0 35 16111.0 36 31381.0 37 82124.0 38 162127.0 39 60286.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80207331461423 20.155825655511922 14.180570730844613 23.86153029902924 2 15.325253404939884 21.55647482884583 40.07354186322181 23.044729902992476 3 16.948738418366112 26.122118199192006 32.90103297288144 24.02811040956044 4 11.806130101361976 19.376829474804655 38.87147107917846 29.94556934465491 5 12.418898323535815 37.98171129980405 36.54885453709751 13.050535839562619 6 28.840264170113944 39.27304835861335 18.36345162929095 13.523235841981759 7 24.53733942956673 33.86989863802405 23.55129786873745 18.04146406367177 8 24.25550959189104 35.27445145995113 21.967002927159687 18.503036020998138 9 24.95512494859327 15.110191837820839 21.61017974211965 38.324503471466244 10-11 22.647628032997073 26.462733144640392 31.135423470498587 19.754215351863948 12-13 24.002104652006675 24.935046084621526 29.89694462587996 21.165904637491835 14-15 21.372499213779424 26.6060672037158 27.53658949609309 24.484844086411687 16-17 20.700583012797253 29.211118368531825 28.436751578489005 21.65154704018192 18-19 20.645305658369015 27.909741877736653 30.60575271548492 20.839199748409413 20-21 21.211868302005467 27.378861552604207 30.44717807291289 20.962092072477443 22-23 20.779446984541693 27.80523501947408 30.18615284128021 21.22916515470402 24-25 21.069501899025088 27.72552434864649 30.37375716670295 20.83121658562547 26-27 21.06732467281128 27.794953673464452 30.39298933159155 20.744732322132712 28-29 21.03696446282991 27.44780704937465 30.414277765682073 21.10095072211336 30-31 20.87258388368774 27.149768972107314 30.751989742845392 21.22565740135956 32-33 20.65159542298667 27.729153059002833 30.364322519776472 21.25492899823403 34-35 21.24162372692745 27.625371942811526 30.140914918837847 20.99208941142318 36-37 21.058010982896676 27.657183636935436 30.37387812371483 20.910927256453057 38-39 20.930885163412924 27.425430002177226 30.369523671287226 21.274161163122628 40-41 21.07361443742894 27.54227447565135 30.31013377845514 21.073977308464574 42-43 21.37487496023524 27.47064665062771 30.192552165924592 20.96192622321246 44-45 21.0209026065058 27.979446959681347 29.866136713037612 21.133513720775237 46-47 21.450136440169533 27.635786998509804 29.280568403940315 21.633508157380348 48-49 21.31070403862995 27.737457603336573 29.07903076751871 21.87280759051476 50-51 21.204123252443978 27.87102377329369 29.299792192334586 21.62506078192775 52-53 21.067967858875157 28.027080216128862 29.623371140966388 21.28158078402959 54-55 21.115586520550597 27.91687834143745 29.234221157800516 21.733313980211435 56-57 21.362217867769793 28.17729878801074 28.62665408713743 21.833829257082034 58-59 20.850690664537826 28.275032053608147 29.369209183056345 21.505068098797686 60-61 21.440839925490483 28.517792776447248 28.67927038730435 21.362096910757916 62-63 20.78936545951569 28.93848126375886 28.62907322737499 21.64308004935046 64-65 20.788397803420665 28.830829523187457 28.67564167694801 21.705130996443863 66-67 20.79396182596705 28.836393545733845 28.562909741877736 21.806734886421367 68-69 20.881272860985852 28.85262042538368 28.382337287840375 21.883769425790096 70-71 20.835812952076832 28.47098241285047 28.60996201949827 22.083242615574424 72-73 20.93657014297119 28.425381619372477 28.355831337542636 22.2822169001137 74-75 20.869801872414545 28.353049326269442 28.28325713041585 22.49389167090016 76-77 20.85867382732177 28.270556644168664 28.39151365604664 22.47925587246293 78-79 20.761424389771875 28.38038561095387 28.264024965527252 22.594165033747007 80-81 20.83230519873237 28.04581851609938 28.281442775237682 22.840433509930573 82-83 20.736144374289378 28.192297457483612 28.24733289788809 22.82422527033892 84-85 20.929675593294142 27.856399835498465 28.2527759634226 22.961148607784793 86-87 20.748723903524688 27.846602317536345 28.216488859859208 23.18818491907976 88-89 20.749570602607832 27.728427316931565 28.219754699179912 23.302247381280694 90-91 20.80581561313109 27.633838933642984 28.157703752086512 23.402641701139416 92-93 20.632726129133705 27.4497423615647 28.28120086121392 23.636330648087668 94-95 20.53257372329874 27.34934804170598 28.326801654691923 23.79127658030336 96-97 20.382224157534413 27.290079105885766 28.32752739676319 24.00016933981663 98-99 20.49062642502652 27.224432378295933 28.28885537031639 23.99608582636116 100-101 20.469797034134068 27.15763117787938 28.169799453274308 24.202772334712243 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 372.0 1 235.5 2 117.0 3 153.0 4 129.5 5 100.5 6 143.0 7 255.5 8 460.5 9 584.0 10 497.0 11 401.0 12 416.5 13 445.5 14 487.0 15 548.5 16 560.0 17 583.0 18 670.5 19 760.5 20 840.5 21 973.0 22 1159.5 23 1427.0 24 1763.5 25 2138.0 26 2710.5 27 3409.0 28 4236.5 29 5134.5 30 6067.5 31 6950.0 32 7822.5 33 8813.0 34 9734.5 35 10543.5 36 11581.5 37 12567.5 38 13230.5 39 13798.5 40 14467.0 41 15241.5 42 15817.5 43 16131.5 44 16326.5 45 16569.5 46 16439.5 47 16198.5 48 15986.0 49 15781.5 50 15269.0 51 14208.0 52 13330.5 53 12565.0 54 11432.5 55 10172.0 56 8680.0 57 7249.0 58 6255.0 59 5234.0 60 4231.5 61 3303.0 62 2586.0 63 1995.5 64 1397.0 65 972.5 66 730.5 67 547.5 68 398.5 69 278.0 70 221.5 71 191.0 72 137.5 73 116.5 74 94.5 75 66.5 76 42.0 77 23.5 78 12.5 79 9.0 80 10.0 81 7.0 82 4.5 83 2.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14539032827733023 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 3.628710356339357E-4 44-45 1.2095701187797855E-4 46-47 4.838280475119142E-4 48-49 0.0029029682850714856 50-51 0.0016933981662916998 52-53 0.0013305271306577643 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.838280475119142E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.2095701187797855E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 413370.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.06726064672343 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8383612553376 58.335703514510186 2 13.799740670837352 20.166184969065256 3 3.722843400508817 8.160539072757357 4 1.332339502821248 3.894015908902641 5 0.5732729154581946 2.0943740767745473 6 0.2785370302269363 1.2211162672413505 7 0.14595742143214324 0.7465296268574246 8 0.08259691056572481 0.4828103994335935 9 0.0593800622397146 0.39048646363990974 >10 0.1559902725675787 1.6755663456167091 >50 0.004991162744259419 0.26962790852727203 >100 0.00399293017363147 0.5195625231969144 >500 9.982325434078668E-4 0.5283150972891411 >1k 9.982325434078668E-4 1.5151678261877255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2829 0.6843747732056028 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1791 0.43326801654691927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.38996540629460286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 949 0.2295764085444033 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 677 0.16377579408278298 RNA PCR Primer, Index 31 (95% over 22bp) ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 547 0.13232697099450855 RNA PCR Primer, Index 31 (95% over 24bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.2095701187797857E-4 0.0 20-21 0.0 0.0 0.0 3.628710356339357E-4 0.0 22-23 0.0 0.0 0.0 8.4669908314585E-4 0.0 24-25 0.0 0.0 0.0 0.00254009724943755 0.0 26-27 0.0 0.0 0.0 0.0044754094394852065 0.0 28-29 0.0 0.0 0.0 0.012821443259065729 0.0 30-31 0.0 0.0 0.0 0.030481166993250597 0.0 32-33 0.0 0.0 0.0 0.05636596753513801 0.0 34-35 0.0 0.0 0.0 0.10124101894186806 0.0 36-37 0.0 0.0 0.0 0.16933981662917 0.0 38-39 0.0 0.0 0.0 0.2629605438227254 0.0 40-41 0.0 0.0 0.0 0.3642015627645935 0.0 42-43 0.0 0.0 0.0 0.451532525340494 0.0 44-45 0.0 0.0 0.0 0.5617243631613325 0.0 46-47 0.0 0.0 0.0 0.7043326801654692 1.2095701187797857E-4 48-49 0.0 0.0 0.0 0.8427075017538767 2.4191402375595714E-4 50-51 0.0 0.0 0.0 0.9916055833756683 2.4191402375595714E-4 52-53 0.0 0.0 0.0 1.1253840385127125 2.4191402375595714E-4 54-55 0.0 0.0 0.0 1.2879502624767158 2.4191402375595714E-4 56-57 0.0 0.0 0.0 1.483537750683407 2.4191402375595714E-4 58-59 0.0 0.0 0.0 1.7126303311802986 2.4191402375595714E-4 60-61 0.0 0.0 0.0 1.9503108595205263 2.4191402375595714E-4 62-63 0.0 0.0 0.0 2.1722669763166174 2.4191402375595714E-4 64-65 0.0 0.0 0.0 2.4176887534170355 2.4191402375595714E-4 66-67 0.0 0.0 0.0 2.7073807968647943 2.4191402375595714E-4 68-69 0.0 0.0 0.0 3.059002830394078 2.4191402375595714E-4 70-71 0.0 0.0 0.0 3.4222367370636477 2.4191402375595714E-4 72-73 0.0 0.0 0.0 3.7947843336478213 2.4191402375595714E-4 74-75 0.0 0.0 0.0 4.187289837191862 2.4191402375595714E-4 76-77 0.0 0.0 0.0 4.611002249800421 2.4191402375595714E-4 78-79 0.0 0.0 0.0 5.088177661659047 2.4191402375595714E-4 80-81 0.0 0.0 0.0 5.610711952971914 2.4191402375595714E-4 82-83 0.0 0.0 0.0 6.156953818612864 2.4191402375595714E-4 84-85 0.0 0.0 0.0 6.740329486900356 2.4191402375595714E-4 86-87 0.0 0.0 0.0 7.366765851416407 2.4191402375595714E-4 88-89 0.0 0.0 0.0 8.071703316641266 2.4191402375595714E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 830 0.0 61.894947 1 GTATCAA 1395 0.0 38.531307 1 TCAACGC 1820 0.0 30.012703 4 ATCAACG 1810 0.0 29.916098 3 CAACGCA 1845 0.0 29.348585 5 TATCAAC 1880 0.0 28.8022 2 AACGCAG 1880 0.0 28.8022 6 CGCAGAG 2040 0.0 26.077534 8 ACGCAGA 2080 0.0 26.032759 7 GCAGAGT 2230 0.0 23.855682 9 TAAGGGT 125 2.2530353E-4 22.799217 4 TACATGG 945 0.0 22.618269 2 GTACATG 955 0.0 22.413965 1 GAGTACT 1760 0.0 22.129921 12-13 CAGAGTA 2225 0.0 22.094746 10-11 GTAAGGG 130 2.937782E-4 21.922323 3 AGAGTAC 2110 0.0 21.723185 10-11 GTCTAAT 135 3.754134E-4 21.141073 1 GTACAAG 250 9.291398E-9 20.92966 1 CCGTCTT 730 0.0 20.82372 46-47 >>END_MODULE