FastQCFastQC Report
Fri 10 Feb 2017
SRR2031826_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031826_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162243
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40200.3458829177719289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37200.3200707597292477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.1799107415574884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.061.0373041
GTATCAA35650.041.1709751
ATCAACG41350.035.0361633
TCAACGC41800.034.7726174
CAACGCA42000.034.3808485
AACGCAG43650.033.2988626
TATCAAC43700.033.152072
ACGCAGA49000.029.3723587
CGCAGAG49350.028.7790378
GCAGAGT55650.025.1796139
GAGTACT41750.024.51788712-13
GTACATG24800.023.5583951
CAGAGTA53150.023.54884310-11
GTGGTAT8600.022.6452771
AGAGTAC49200.021.96385410-11
GTACTTT48500.020.95869614-15
TACATGG25850.020.947712
ACATGGG25850.020.5802063
TGGTATC9000.020.0554922
AGTACTT46100.019.01024212-13
Produced by FastQC (version 0.11.5)