FastQCFastQC Report
Fri 10 Feb 2017
SRR2031826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162243
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43970.3783201963788984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41300.3553473757209121No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT25190.21673608703171365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24980.21492923596872598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.060.392971
GTATCAA32550.040.927741
CAACGCA38950.034.1453825
TCAACGC39250.033.8843964
ATCAACG39450.033.833023
AACGCAG40050.033.088966
TATCAAC41400.032.2394332
ACGCAGA46250.028.5505497
CGCAGAG46900.028.0535838
GAGTCGA908.9490745E-426.3881479
GCAGAGT53850.024.0801039
GAGTACT40950.022.90839212-13
CAGAGTA51500.022.55033910-11
GTACATG22750.021.9593071
AGAGTAC48950.021.29919810-11
ACATGGG21700.020.7943483
GTATACG1150.003687850720.6863021
GTACTTT47000.019.70689414-15
TATACTG5800.019.654625
TACATGG24200.019.6275462
Produced by FastQC (version 0.11.5)