FastQCFastQC Report
Fri 10 Feb 2017
SRR2031825_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031825_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775530
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.34789111962142016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26840.3460859025440666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16590.21391822366639587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.058.0178571
GTATCAA23750.038.41
ATCAACG28900.031.2283743
TCAACGC28800.031.0069454
CAACGCA29800.030.1258375
TATCAAC30400.029.843752
AACGCAG30850.028.9465146
GCGTAAG908.9449127E-426.3888871
CGCAGAG34450.025.5079828
ACGCAGA34800.025.387937
GTACATG20450.022.5305631
GCAGAGT38300.022.447789
TACATGG20400.022.1200982
GAGTACT25600.022.0800812-13
CAGAGTA36200.021.91298310-11
GTACTAG1150.00372220120.6521761
ATAAACG1150.00372220120.6521763
ATAGTAC1404.849781E-420.3571413
AGAGTAC35400.020.06002810-11
GTATAAA3353.45608E-1119.8507461
Produced by FastQC (version 0.11.5)