FastQCFastQC Report
Fri 10 Feb 2017
SRR2031825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775530
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.3896690005544595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29510.3805139710907379No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT18910.24383324951968333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.22990728920867018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.056.9513741
GTATCAA24950.038.8871081
CAACGCA30350.031.7704225
TCAACGC30350.031.7704224
ATCAACG30650.031.4594573
TATCAAC32300.030.4406192
AACGCAG31450.030.3571536
ACGCAGA34750.027.3376277
CGCAGAG35500.026.3586718
TACACCG950.001226325624.9995445
GAGTACT27800.023.66413512-13
CAGAGTA37700.023.4975810-11
GCAGAGT40800.022.701799
GTGGTAT5950.022.3813921
GTACTTT29800.021.8368514-15
AGAGTAC35050.021.0730810-11
GTACATG18550.020.5112481
TACATGG18350.020.4492172
AGTACTT29150.019.47219812-13
ACTTTTT34300.019.4566416-17
Produced by FastQC (version 0.11.5)