FastQCFastQC Report
Fri 10 Feb 2017
SRR2031823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38662
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT16544.278102529615643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8852.2890693704412604No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6981.8053903057265532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6881.7795251151000984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6061.5674305519631682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.941492938802959No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT3610.9337333816150225RNA PCR Primer, Index 31 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.8716569241115307No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT2470.6388702084734365RNA PCR Primer, Index 31 (95% over 24bp)
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.5897263462831721No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.5173038125290984No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.32590140189333194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.2793440587657131No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT990.2560653872019037No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG910.23537323470073976RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT890.23020019657544877RNA PCR Primer, Index 31 (96% over 27bp)
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.23020019657544877No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.20692152501163935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.1836428534478299No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT700.18105633438518443No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.15001810563343854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC520.13449899125756556No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.12156639594433812No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT460.1189798768816926No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT450.11639335781904712No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.11122031969375615No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG430.11122031969375615RNA PCR Primer, Index 38 (95% over 24bp)
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG420.10863380063111064No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC410.10604728156846516No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.10604728156846516No Hit
GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.10087424344317418No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAA200.00205889871.257
GGATTGA200.00205889871.256
CGCAGAA656.4210326E-1058.4615339
GGTATCA3750.043.0666661
GTATCAA5400.029.9074061
CATGGGG650.005775376729.2307664
AACGCAG5850.027.6068386
TATACAC5550.027.3873883
AGGATTG700.00829336927.1428595
GCAGAAA705.689162E-727.14285910-11
GGCTACA350.00834408327.14285928-29
CAACGCA6000.026.9166665
TTATACA5700.026.6666662
TCAACGC6100.026.4754094
CTTATAC5950.026.3445381
CGCAGAG5600.026.2946438
ACGCAGA6150.026.2601627
CATCTCC5800.026.2068969
TACACAT6050.025.909095
TATCAAC6250.025.842
Produced by FastQC (version 0.11.5)