##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031823_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38662 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7746365940717 34.0 31.0 34.0 31.0 34.0 2 32.88896073664063 34.0 33.0 34.0 31.0 34.0 3 32.863897366923595 34.0 33.0 34.0 31.0 34.0 4 36.274740054834204 37.0 37.0 37.0 35.0 37.0 5 36.33371268946252 37.0 37.0 37.0 35.0 37.0 6 36.4542444777818 37.0 37.0 37.0 35.0 37.0 7 36.40471263773214 37.0 37.0 37.0 35.0 37.0 8 36.44330350214681 37.0 37.0 37.0 35.0 37.0 9 38.22194920076561 39.0 39.0 39.0 37.0 39.0 10-11 38.23079509595986 39.0 39.0 39.0 37.0 39.0 12-13 38.22934664528478 39.0 39.0 39.0 37.0 39.0 14-15 39.67699549945683 41.0 40.0 41.0 37.0 41.0 16-17 39.606202472712226 41.0 39.5 41.0 37.0 41.0 18-19 39.577569706688735 41.0 39.5 41.0 36.0 41.0 20-21 39.55738192540479 41.0 40.0 41.0 35.0 41.0 22-23 39.44591588640008 41.0 39.0 41.0 35.0 41.0 24-25 39.29058248409291 41.0 39.0 41.0 35.0 41.0 26-27 39.06856862035073 41.0 38.5 41.0 35.0 41.0 28-29 38.89787129481144 41.0 38.0 41.0 35.0 41.0 30-31 38.579962236821686 40.0 37.5 41.0 35.0 41.0 32-33 37.961253944441566 40.0 35.0 41.0 35.0 41.0 34-35 37.83190212611867 40.0 35.0 41.0 35.0 41.0 36-37 37.63100719052299 40.0 35.0 41.0 34.0 41.0 38-39 37.35309864983705 40.0 35.0 41.0 33.5 41.0 40-41 37.25215974341731 40.0 35.0 41.0 33.0 41.0 42-43 36.99004190160881 40.0 35.0 41.0 33.0 41.0 44-45 36.62463142103357 40.0 35.0 41.0 31.5 41.0 46-47 36.1837075164244 40.0 35.0 41.0 29.0 41.0 48-49 35.80842946562516 39.5 35.0 41.0 26.0 41.0 50-51 35.387835600848376 39.0 35.0 41.0 23.0 41.0 52-53 35.41262738606383 39.0 35.0 41.0 22.5 41.0 54-55 35.314999224044286 39.0 35.0 41.0 21.0 41.0 56-57 35.013527494697634 39.0 35.0 41.0 20.0 41.0 58-59 34.79910506440432 39.0 35.0 41.0 18.0 41.0 60-61 34.41605452382184 38.0 35.0 41.0 16.5 41.0 62-63 34.05341161864363 37.0 35.0 41.0 14.5 41.0 64-65 33.6564326729088 37.0 35.0 40.5 10.0 41.0 66-67 33.14159898608453 36.0 34.0 40.0 10.0 41.0 68-69 32.343308675184936 36.0 33.0 39.0 9.0 41.0 70-71 31.651570017071023 35.0 32.5 39.0 7.5 41.0 72-73 30.983135895711552 35.0 31.5 38.5 7.0 41.0 74-75 30.651699343024156 35.0 31.0 37.0 7.0 40.0 76-77 29.445540841136 34.5 28.5 36.0 7.0 39.0 78-79 30.027753349542188 35.0 30.0 36.0 7.0 39.0 80-81 29.887059645129582 35.0 30.0 36.0 7.0 38.0 82-83 29.61583725622058 35.0 29.0 36.0 7.0 37.0 84-85 29.405643784594695 35.0 29.0 35.0 7.0 37.0 86-87 29.197480730432982 35.0 29.0 35.0 7.0 36.5 88-89 29.02730070870622 35.0 29.0 35.0 7.0 36.0 90-91 28.794410532305623 35.0 27.0 35.0 7.0 36.0 92-93 28.67306399048161 35.0 27.0 35.0 7.0 36.0 94-95 28.560847860948734 35.0 25.5 35.0 7.0 35.0 96-97 28.424939216802027 35.0 25.0 35.0 7.0 35.0 98-99 28.339183177280013 35.0 25.0 35.0 7.0 35.0 100-101 27.47378562930009 34.0 22.5 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.693208663532296 1101 2 0.004803507809285179 1101 3 0.1590316580448814 1101 4 -0.06222674694713248 1101 5 -0.8664890714237004 1101 6 -0.3784738987532634 1101 7 -0.39288588045297956 1101 8 -0.2883299318846184 1101 9 0.13421285706524344 1101 10-11 -0.6933983471429812 1101 12-13 -0.6240127718015742 1101 14-15 -1.2681483442172379 1101 16-17 -0.46503002148156725 1101 18-19 -0.5328109544596984 1101 20-21 -0.4551324382316011 1101 22-23 -1.322417802882903 1101 24-25 -0.3602798073567115 1101 26-27 -0.18198599204099963 1101 28-29 0.27108189305748454 1101 30-31 0.2525491282686261 1101 32-33 -0.29742233690854647 1101 34-35 0.3472080650069671 1101 36-37 0.39926385160486433 1101 38-39 0.5089671713654411 1101 40-41 0.8160941488736242 1101 42-43 0.3430955383935199 1101 44-45 0.23647776506327034 1101 46-47 0.5699103701932202 1101 48-49 1.128798413691392 1101 50-51 1.2734354202977158 1101 52-53 0.8143787697434703 1101 54-55 0.39464454192521004 1101 56-57 0.7585332295276501 1101 58-59 0.06446285355671222 1101 60-61 0.1286218256839078 1101 62-63 -0.15034505593480674 1101 64-65 0.04216349394747709 1101 66-67 -0.2938342213381233 1101 68-69 -1.1706643949230369 1101 70-71 -0.6104150725703548 1101 72-73 -2.0321435154696132 1101 74-75 -1.4515423136850458 1101 76-77 -2.293621328997215 1101 78-79 -2.5955705956450643 1101 80-81 -1.6732144553644446 1101 82-83 -1.647226123821003 1101 84-85 -0.43992319809527913 1101 86-87 -0.5242560352756946 1101 88-89 -1.5547011551400232 1101 90-91 -1.2669996176587617 1101 92-93 -1.5504980823320622 1101 94-95 -1.0149045508797592 1101 96-97 -0.9189914971106781 1101 98-99 -2.2332276455935123 1101 100-101 -1.9873084847349851 1102 1 0.26111825954462375 1102 2 0.6273996616554385 1102 3 0.6205701195833413 1102 4 0.269504022283634 1102 5 0.46043400549937985 1102 6 0.35710302432365637 1102 7 0.26336411954702044 1102 8 0.21407391426922828 1102 9 0.40584747244985664 1102 10-11 0.3546785759339386 1102 12-13 0.2533910743522725 1102 14-15 0.4313708865519885 1102 16-17 -0.18017425225080075 1102 18-19 -0.09050326215200499 1102 20-21 -0.3325362843854478 1102 22-23 -0.027946649036749704 1102 24-25 -0.5958567304336313 1102 26-27 -0.33222637665638644 1102 28-29 0.18694727767287134 1102 30-31 0.018174128268626077 1102 32-33 0.03551035539914693 1102 34-35 0.0683619111608138 1102 36-37 0.4689753900663973 1102 38-39 1.0594479405962147 1102 40-41 0.9134499181043978 1102 42-43 1.8551147691627463 1102 44-45 0.8855162266017373 1102 46-47 0.7225546009624466 1102 48-49 2.1636541829221656 1102 50-51 2.3227142664515625 1102 52-53 2.528321077435777 1102 54-55 2.3814233880790567 1102 56-57 1.5554082295276501 1102 58-59 2.1005205458644056 1102 60-61 2.890641056453134 1102 62-63 2.1609530209882735 1102 64-65 2.6623558016397837 1102 66-67 2.1520792402003366 1102 68-69 2.1406336820000433 1102 70-71 0.9232387735834919 1102 72-73 2.41016417683808 1102 74-75 2.0340346093918775 1102 76-77 2.581378671002785 1102 78-79 2.2409678658933956 1102 80-81 1.8147663138663255 1102 82-83 2.3419565684866903 1102 84-85 2.087721032673951 1102 86-87 2.682474733955072 1102 88-89 2.1436161525522834 1102 90-91 2.309923459264315 1102 92-93 2.5264249945910144 1102 94-95 2.7903839106587007 1102 96-97 3.112258502889322 1102 98-99 3.615330046714181 1102 100-101 2.8684607460342484 1103 1 0.036319872447847956 1103 2 -0.22844711253811312 1103 3 0.6044410873252772 1103 4 0.41668144163847387 1103 5 0.4523694893703478 1103 6 0.447828830775272 1103 7 -0.005789106259427967 1103 8 0.33201746265632437 1103 9 0.3302426337401769 1103 10-11 0.47161405980490656 1103 12-13 -0.035923441776759546 1103 14-15 0.24487895106811663 1103 16-17 0.6590394574266227 1103 18-19 0.28046447978348255 1103 20-21 0.045487909162936546 1103 22-23 0.3394928670922823 1103 24-25 0.7166432695663687 1103 26-27 0.7237212039887737 1103 28-29 0.575052116382551 1103 30-31 0.6799684831073378 1103 32-33 0.6448853553991469 1103 34-35 0.983684491805974 1103 36-37 1.1635318416792941 1103 38-39 1.0362624567252468 1103 40-41 0.6095184664914939 1103 42-43 0.8641873498079065 1103 44-45 1.0125323556339936 1103 46-47 -0.44125588290852136 1103 48-49 0.05125498937378126 1103 50-51 0.45829894387092196 1103 52-53 0.12207107743577694 1103 54-55 -0.2914596764370714 1103 56-57 -0.4108216091820296 1103 58-59 -0.630326228329146 1103 60-61 0.3029394435499029 1103 62-63 0.6685135048592414 1103 64-65 0.6724364468010791 1103 66-67 -0.017779630767407184 1103 68-69 1.043355456193595 1103 70-71 0.4454161929383318 1103 72-73 0.5069383703864645 1103 74-75 1.0441152545531693 1103 76-77 0.8112173806802012 1103 78-79 0.9466130271837159 1103 80-81 1.2734356687050372 1103 82-83 1.5531460846157223 1103 84-85 1.105362161706207 1103 86-87 0.7611037662131359 1103 88-89 1.2610556686813155 1103 90-91 0.49540733023205874 1103 92-93 0.5183604784619824 1103 94-95 0.8337306848522523 1103 96-97 -0.06415278743325814 1103 98-99 -0.581242533930979 1103 100-101 -1.1194424797722036 1104 1 0.22986825954462375 1104 2 0.615792518798294 1104 3 0.7205701195833427 1104 4 0.3641468794264924 1104 5 0.39614829121366313 1104 6 0.11603159575222577 1104 7 0.20711411954702186 1104 8 0.2721096285549436 1104 9 0.49245461530699686 1104 10-11 0.32967857593394 1104 12-13 0.5078553600665572 1104 14-15 0.45547802940913584 1104 16-17 0.4042007477492007 1104 18-19 0.7380681664194242 1104 20-21 0.648713715614555 1104 22-23 0.8528569223918225 1104 24-25 0.7309289838520883 1104 26-27 1.0191129090579025 1104 28-29 0.555697277672877 1104 30-31 0.8596919854114873 1104 32-33 1.059171069684858 1104 34-35 0.6933619111608138 1104 36-37 1.0805825329235361 1104 38-39 0.5781979405962119 1104 40-41 1.0924677752472505 1104 42-43 0.5167219120198894 1104 44-45 0.6623019408874526 1104 46-47 0.6721081723910132 1104 48-49 -0.8948279599349789 1104 50-51 -0.09469644783415276 1104 52-53 0.2506425060072104 1104 54-55 0.3693698166504902 1104 56-57 -0.10575248475806376 1104 58-59 0.2710562601501181 1104 60-61 -0.8334660864040089 1104 62-63 0.6122923067025567 1104 64-65 0.2069986587826449 1104 66-67 0.6748470973431964 1104 68-69 0.7276872534286127 1104 70-71 0.5071673450120642 1104 72-73 0.5673070339809385 1104 74-75 0.5501060379633067 1104 76-77 1.2420929567170695 1104 78-79 1.0802535801791109 1104 80-81 0.8960163138663226 1104 82-83 1.0379387113438305 1104 84-85 1.1408460326739522 1104 86-87 0.9306890196693551 1104 88-89 1.3601340096951375 1104 90-91 1.3027806021214552 1104 92-93 1.4478535660195853 1104 94-95 0.9180624820872723 1104 96-97 0.7810085028893212 1104 98-99 1.5947943324284672 1104 100-101 1.2363178888913886 1105 1 0.34751531836815275 1105 2 0.7762967204789675 1105 3 0.9146877666421673 1105 4 0.471709904636576 1105 5 0.35749282902879287 1105 6 0.25048537726483033 1105 7 0.37182000189996245 1105 8 0.4401768554456993 1105 9 0.39665629597926966 1105 10-11 0.5679138700515836 1105 12-13 0.6339057802346204 1105 14-15 0.5655620630225755 1105 16-17 0.6571419242197862 1105 18-19 0.703614384906821 1105 20-21 0.8016548920851392 1105 22-23 0.9784871744926562 1105 24-25 0.9372315048604847 1105 26-27 1.0951633292259615 1105 28-29 1.2880502188493423 1105 30-31 1.679019716503916 1105 32-33 1.939003002457973 1105 34-35 1.5757148523372848 1105 36-37 2.3053724488899263 1105 38-39 2.895844999419751 1105 40-41 2.570619035751456 1105 42-43 2.7209235926921522 1105 44-45 3.1686044618958533 1105 46-47 3.0065619539036206 1105 48-49 3.8097938888045206 1105 50-51 4.531353972333918 1105 52-53 4.563247548024009 1105 54-55 3.814327799843767 1105 56-57 4.361474405998237 1105 58-59 4.374417604687935 1105 60-61 4.315273409394308 1105 62-63 3.9118721386353315 1105 64-65 4.629267566345668 1105 66-67 4.167704240200337 1105 68-69 4.891552799647101 1105 70-71 5.412209361818782 1105 72-73 5.321928882720435 1105 74-75 4.8538875505683485 1105 76-77 4.235790435708669 1105 78-79 4.365967865893396 1105 80-81 5.037192784454557 1105 82-83 5.148022744957284 1105 84-85 5.099669562085719 1105 86-87 4.244974733955072 1105 88-89 4.0176970349052255 1105 90-91 4.045217576911373 1105 92-93 3.9087779357674854 1105 94-95 4.196633910658701 1105 96-97 4.286890855830496 1105 98-99 4.267903576125949 1105 100-101 4.111107804857777 1106 1 0.34415397383033763 1106 2 0.4729353759411552 1106 3 0.2919986910119121 1106 4 0.19271830799792156 1106 5 0.42471971978509515 1106 6 0.3160315957522286 1106 7 0.4071141195470176 1106 8 0.1578239142692297 1106 9 0.2638831867355691 1106 10-11 -0.18460713835177245 1106 12-13 0.13642678863798352 1106 14-15 0.19833517226626896 1106 16-17 0.532772176320627 1106 18-19 0.3666395949908505 1106 20-21 0.591570858471691 1106 22-23 0.6671426366775393 1106 24-25 0.7309289838520883 1106 26-27 0.8905414804864691 1106 28-29 0.8699829919585866 1106 30-31 0.6596919854114844 1106 32-33 1.059171069684858 1106 34-35 0.9219333397322416 1106 36-37 0.9091539614949724 1106 38-39 0.7496265120247898 1106 40-41 0.17818206096153943 1106 42-43 0.17386476916274773 1106 44-45 0.019444798030306742 1106 46-47 -0.32789182760898683 1106 48-49 0.06231489720787664 1106 50-51 0.04816069502299314 1106 52-53 0.2506425060072033 1106 54-55 0.04079838807905389 1106 56-57 0.4799618009562252 1106 58-59 0.4853419744358334 1106 60-61 -0.27632322926115194 1106 62-63 -1.2448505504402974 1106 64-65 -1.464429912645933 1106 66-67 -0.95372433122823 1106 68-69 -1.4008841751428136 1106 70-71 -2.0928326549879372 1106 72-73 -1.7184072517333462 1106 74-75 -1.678465390608121 1106 76-77 -1.6721927575686415 1106 78-79 -1.2197464198208898 1106 80-81 -0.6039836861336738 1106 82-83 -1.4906327172275944 1106 84-85 -1.0591539673260506 1106 86-87 -1.3407395517592136 1106 88-89 -1.3684374188762867 1106 90-91 -1.8400765407356836 1106 92-93 -1.6664321482661286 1106 94-95 -1.9247946607698694 1106 96-97 -1.5047057828249635 1106 98-99 -1.9766342390001022 1106 100-101 -1.8065392539657523 1107 1 0.46063749031385726 1107 2 0.415059918065694 1107 3 0.4282624272756479 1107 4 0.19418350946312302 1107 5 0.22325451831989085 1107 6 0.21126969099032067 1107 7 0.22249873493163363 1107 8 -0.03191967547436292 1107 9 0.31370003655241874 1107 10-11 0.16557601183136939 1107 12-13 0.11957697178817028 1107 14-15 0.3536465275776308 1107 16-17 0.26573920928765915 1107 18-19 -0.052041723690472 1107 20-21 -0.4102606433598126 1107 22-23 -0.021135751600851904 1107 24-25 0.1012586541817555 1107 26-27 0.16416785411284707 1107 28-29 0.16851779049338234 1107 30-31 0.4256260513455459 1107 32-33 0.6833468938606799 1107 34-35 0.5138747316736314 1107 36-37 0.6300330823740907 1107 38-39 0.2781979405962147 1107 40-41 0.5019915847710692 1107 42-43 -0.7338275385295603 1107 44-45 0.07622135480686154 1107 46-47 0.2109360112188554 1107 48-49 0.4813625162554942 1107 50-51 1.0042046510669493 1107 52-53 1.083609538974244 1107 54-55 1.0356701829508523 1107 56-57 0.5598152808097012 1107 58-59 0.2952320843259386 1107 60-61 0.21195515901723638 1107 62-63 1.1060652004754559 1107 64-65 1.516522468306455 1107 66-67 1.597191419687519 1107 68-69 1.00241252815389 1107 70-71 1.0690721069168205 1107 72-73 1.858882125556029 1107 74-75 1.183073070930341 1107 76-77 1.4563786710027848 1107 78-79 1.0967370966626255 1107 80-81 1.0831958010458145 1107 82-83 0.8848251582302815 1107 84-85 0.7203332121611297 1107 86-87 0.2193337083140463 1107 88-89 0.41324756280869224 1107 90-91 0.8163337156745705 1107 92-93 1.3148865330525545 1107 94-95 1.3440698080945985 1107 96-97 1.2348546567354752 1107 98-99 1.2347210723552102 1107 100-101 1.6473068998804017 1108 1 -0.4451317404553734 1108 2 -0.06635033834456294 1108 3 -0.7044298804166615 1108 4 0.007004022283631173 1108 5 -0.06456599450061873 1108 6 -0.05539697567634505 1108 7 0.10711411954702044 1108 8 0.032823914269229704 1108 9 -0.05040252755014052 1108 10-11 -0.47032142406606425 1108 12-13 -0.1778589256477261 1108 14-15 -0.33737911344800864 1108 16-17 0.004200747749202094 1108 18-19 0.29699673784799785 1108 20-21 0.12371371561454936 1108 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2316 52-53 -0.4797807744160707 2316 54-55 0.33339098067165196 2316 56-57 0.5826073036017263 2316 58-59 0.22899276808662705 2316 60-61 0.5723540194160961 2316 62-63 0.4080595024697544 2316 64-65 -0.38162567984169726 2316 66-67 0.1769634994595961 2316 68-69 -2.0937413179999567 2316 70-71 -0.8105575227128057 2316 72-73 -0.8583543416804389 2316 74-75 -0.8432802054229391 2316 76-77 -0.25658429196017707 2316 78-79 -0.43032843040290203 2316 80-81 0.049720017570027863 2316 82-83 -0.890103616698493 2316 84-85 -0.4961910043630873 2316 86-87 -0.8013215623412258 2316 88-89 -0.2562681067069761 2316 90-91 -0.6530395036986469 2316 92-93 -0.39024167207564986 2316 94-95 -0.6644772004524135 2316 96-97 0.13656405844487907 2316 98-99 0.17204300967714303 2316 100-101 0.289757042330546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 7.0 15 21.0 16 90.0 17 202.0 18 339.0 19 471.0 20 569.0 21 684.0 22 770.0 23 678.0 24 606.0 25 610.0 26 669.0 27 824.0 28 1085.0 29 1069.0 30 667.0 31 529.0 32 559.0 33 697.0 34 1021.0 35 2897.0 36 3055.0 37 5995.0 38 11208.0 39 3327.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.910164749766864 21.210237281110768 15.070977100818567 21.8086208683038 2 15.454451399306812 23.806321452589106 42.09559774455538 18.643629403548704 3 15.963995654647976 29.81739175417723 38.011484144638146 16.20712844653665 4 9.200248305830014 26.403186591485177 37.66489059024365 26.731674512441156 5 8.70880968392737 39.17800424189126 39.50131912472195 12.611866949459419 6 20.730950287103617 42.99053334023072 18.506543893228493 17.771972479437174 7 17.735761212560135 38.583104857482795 24.00548342041281 19.675650509544255 8 21.126687703688376 37.09585639646164 23.232114220681808 18.545341679168175 9 23.12089390098805 15.857948373079509 22.469091097201385 38.552066628731055 10-11 20.14381045988309 28.776317831462418 31.049868087527805 20.03000362112669 12-13 23.250219854120324 28.251254461745383 30.12777404169469 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15.607056024002897 29.382856551652786 36.243598365319954 18.766489059024366 40-41 15.263048988671047 29.27810252961564 37.64807821633646 17.810770265376856 42-43 17.63876674771093 28.915989860845276 36.596658217371065 16.848585174072735 44-45 15.4479851016502 30.85717241736072 36.848843825979 16.84599865501009 46-47 18.329367337437276 28.98453261600538 33.49154208266515 19.194557963892194 48-49 17.88189953959961 28.476281620195543 33.46697015157002 20.174848688634835 50-51 15.242356836169883 28.605607573327816 35.86855310123636 20.283482489265946 52-53 15.716983084165332 29.685479281982307 37.61316020899074 16.98437742486162 54-55 15.382028865552739 29.6622006104185 35.02276136775128 19.933009156277482 56-57 18.126325591019608 29.764368113392997 32.16724432259066 19.94206197299674 58-59 15.777766282137499 30.766644250168124 36.06124877140345 17.394340696290932 60-61 17.702136464745745 33.50447467797837 31.211525528943145 17.58186332833273 62-63 15.275981583984274 35.915110444363975 31.022709637370028 17.786198334281725 64-65 14.975945372717398 35.94097563499043 30.73301950235373 18.35005948993844 66-67 15.270808545858984 34.79385443070716 30.91407583673892 19.021261186694947 68-69 15.322538927111893 34.47053954787647 30.479540634214473 19.727380890797168 70-71 16.176090217784907 34.148517924577106 30.392892245615847 19.282499612022143 72-73 16.015726035900883 32.37028606900833 30.947700584553306 20.666287310537477 74-75 15.839842739640991 32.43494904557447 30.52997775593606 21.19523045884848 76-77 16.101081164968186 32.22026796337489 30.945114065490664 20.73353680616626 78-79 16.33774765920025 31.268428948321347 31.067973720966325 21.325849671512078 80-81 16.25885882778956 30.924421912989498 30.870105012673942 21.946614246546996 82-83 16.10366768403083 30.519631679685478 31.104184987843357 22.27251564844033 84-85 16.06616315762247 29.721690548859346 30.771817288293413 23.44032900522477 86-87 16.223940820443847 30.28943148311003 30.361854016864104 23.12477367958202 88-89 16.37137240701464 29.973876157467284 30.013967202938286 23.640784232579794 90-91 16.526563550773368 28.4853344369148 30.866225234079973 24.121876778231854 92-93 16.344213956856862 29.109978790543682 30.63343851844188 23.91236873415757 94-95 16.368785887951994 28.555170451606227 31.002017484868865 24.074026175572914 96-97 16.408876933423 28.455589467694374 30.83648026485955 24.29905333402307 98-99 15.91485179245771 28.710361595364958 30.179504422947595 25.195282189229733 100-101 15.855361854016865 28.433604055661892 30.76017795251151 24.950856137809733 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1147.0 1 660.0 2 195.0 3 241.5 4 180.5 5 100.5 6 172.0 7 340.0 8 481.5 9 453.5 10 274.0 11 153.0 12 146.0 13 139.0 14 117.0 15 109.0 16 116.0 17 127.0 18 137.0 19 134.5 20 137.5 21 141.5 22 166.0 23 192.0 24 205.5 25 249.0 26 313.0 27 385.0 28 443.0 29 513.0 30 635.0 31 713.5 32 764.0 33 947.5 34 1137.0 35 1305.0 36 1470.0 37 1482.0 38 1406.0 39 1397.0 40 1436.5 41 1463.5 42 1396.0 43 1432.5 44 1472.0 45 1309.5 46 1093.0 47 1065.0 48 1268.0 49 1237.5 50 1070.5 51 964.5 52 839.5 53 641.0 54 457.0 55 397.0 56 381.5 57 333.5 58 272.0 59 221.0 60 189.0 61 182.5 62 179.5 63 140.0 64 84.0 65 69.0 66 49.0 67 29.0 68 29.0 69 27.0 70 18.0 71 13.0 72 9.0 73 9.5 74 6.5 75 6.5 76 6.0 77 2.5 78 2.5 79 2.0 80 2.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15001810563343854 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 38662.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.39216802027831 #Duplication Level Percentage of deduplicated Percentage of total 1 76.68621700879766 37.87698515338058 2 12.232928361960619 12.084217060679737 3 4.205069124423963 6.23092442191299 4 2.215123586091328 4.376390253996172 5 1.241097612065354 3.065025089234908 6 0.7436112274821952 2.203714241373959 7 0.5812735651445329 2.0097253116755467 8 0.39275240888144114 1.5519114375872949 9 0.28278173439463766 1.2570482644457088 >10 1.3039379974863845 10.718534995602917 >50 0.047130289065772935 1.8079768247891987 >100 0.04189359028068705 5.098029072474263 >500 0.020946795140343526 7.441415343231079 >1k 0.005236698785085881 4.278102529615643 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1654 4.278102529615643 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 885 2.2890693704412604 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 1.8053903057265532 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 688 1.7795251151000984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 1.5674305519631682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 364 0.941492938802959 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 361 0.9337333816150225 RNA PCR Primer, Index 31 (95% over 22bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 337 0.8716569241115307 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 247 0.6388702084734365 RNA PCR Primer, Index 31 (95% over 24bp) GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 228 0.5897263462831721 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.5173038125290984 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.32590140189333194 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 108 0.2793440587657131 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 99 0.2560653872019037 No Hit TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 91 0.23537323470073976 RNA PCR Primer, Index 31 (96% over 29bp) CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 89 0.23020019657544877 RNA PCR Primer, Index 31 (96% over 27bp) GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.23020019657544877 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.20692152501163935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.1836428534478299 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 70 0.18105633438518443 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.15001810563343854 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC 52 0.13449899125756556 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.12156639594433812 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 46 0.1189798768816926 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 45 0.11639335781904712 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.11122031969375615 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 43 0.11122031969375615 RNA PCR Primer, Index 38 (95% over 24bp) GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 42 0.10863380063111064 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 41 0.10604728156846516 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 41 0.10604728156846516 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.10087424344317418 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.001293259531322746 0.0 16-17 0.0 0.0 0.0 0.00646629765661373 0.0 18-19 0.0 0.0 0.0 0.007759557187936475 0.0 20-21 0.0 0.0 0.0 0.010346076250581966 0.0 22-23 0.0 0.0 0.0 0.029744969220423155 0.0 24-25 0.0 0.0 0.0 0.04267756453365061 0.0 26-27 0.0 0.0 0.0 0.06466297656613729 0.0 28-29 0.0 0.0 0.0 0.15001810563343854 0.0 30-31 0.0 0.0 0.0 0.2961564326729088 0.0 32-33 0.0 0.0 0.0 0.45393409549428376 0.0 34-35 0.0 0.0 0.0 0.6776679944131188 0.0 36-37 0.0 0.0 0.0 0.9285603434897315 0.0 38-39 0.0 0.0 0.0 1.2324763333505768 0.0 40-41 0.0 0.0 0.0 1.6501991619678238 0.0 42-43 0.0 0.0 0.0 1.8907454347938546 0.0 44-45 0.0 0.0 0.0 2.07697480730433 0.0 46-47 0.0 0.0 0.0 2.2968289276291967 0.0 48-49 0.0 0.0 0.0 2.5179763074853865 0.0 50-51 0.0 0.0 0.0 2.682220267963375 0.0 52-53 0.0 0.0 0.0 2.843877709378718 0.0 54-55 0.0 0.0 0.0 3.0352801200144848 0.0 56-57 0.0 0.0 0.0 3.3676478195644304 0.0 58-59 0.0 0.0 0.0 3.621126687703688 0.0 60-61 0.0 0.0 0.0 3.8733122963116235 0.0 62-63 0.0 0.0 0.0 4.122911385856914 0.0 64-65 0.0 0.0 0.0 4.369923956339559 0.0 66-67 0.0 0.0 0.0 4.699705136826859 0.0 68-69 0.0 0.0 0.0 5.0799234390357455 0.0 70-71 0.0 0.0 0.0 5.438156329212147 0.0 72-73 0.0 0.0 0.0 5.828720707671615 0.0 74-75 0.0 0.0 0.0 6.192126635973307 0.0 76-77 0.0 0.0 0.0 6.581397754901453 0.0 78-79 0.0 0.0 0.0 7.050850964771611 0.0 80-81 0.0 0.0 0.0 7.577207594019968 0.0 82-83 0.0 0.0 0.0 8.142362009208007 0.0 84-85 0.0 0.0 0.0 8.66095908126843 0.0 86-87 0.0 0.0 0.0 9.126532512544618 0.0 88-89 0.0 0.0 0.0 9.69298018726398 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTGAA 20 0.002058898 71.25 7 GGATTGA 20 0.002058898 71.25 6 CGCAGAA 65 6.4210326E-10 58.461533 9 GGTATCA 375 0.0 43.066666 1 GTATCAA 540 0.0 29.907406 1 CATGGGG 65 0.0057753767 29.230766 4 AACGCAG 585 0.0 27.606838 6 TATACAC 555 0.0 27.387388 3 AGGATTG 70 0.008293369 27.142859 5 GCAGAAA 70 5.689162E-7 27.142859 10-11 GGCTACA 35 0.008344083 27.142859 28-29 CAACGCA 600 0.0 26.916666 5 TTATACA 570 0.0 26.666666 2 TCAACGC 610 0.0 26.475409 4 CTTATAC 595 0.0 26.344538 1 CGCAGAG 560 0.0 26.294643 8 ACGCAGA 615 0.0 26.260162 7 CATCTCC 580 0.0 26.206896 9 TACACAT 605 0.0 25.90909 5 TATCAAC 625 0.0 25.84 2 >>END_MODULE