FastQCFastQC Report
Fri 10 Feb 2017
SRR2031819_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031819_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523660
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138740.3937383288966586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107670.3055629657798993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70290.1994800860468944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.061.5074161
GTATCAA113400.045.028661
TCAACGC144350.035.1108444
ATCAACG145850.034.7171753
CAACGCA147650.034.5191355
AACGCAG150300.034.1001326
TATCAAC151350.033.643872
ACGCAGA168750.030.2029617
CGCAGAG170050.029.9162038
GTACATG103900.026.515881
GCAGAGT191200.026.060419
TACATGG105800.025.7254262
ACATGGG106500.024.3075123
AGAGTAC170200.023.48486910-11
GAGTACT129800.023.07299812-13
CAGAGTA183700.022.6769210-11
AGTACTT136750.020.9972612-13
GTACTTT142450.020.99069814-15
CATGGGG66100.020.9114974
ACTTTTT173700.017.2826716-17
Produced by FastQC (version 0.11.5)