FastQCFastQC Report
Fri 10 Feb 2017
SRR2031819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523660
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165140.46866042694244053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128000.36325865719167005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86390.24517121402178416No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT35780.10154214651810901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65000.061.317481
GTATCAA124900.046.1523481
TCAACGC158650.036.1371154
ATCAACG160050.035.731983
CAACGCA162050.035.34965
TATCAAC163200.035.0131952
AACGCAG164300.034.952246
ACGCAGA187150.030.6086247
CGCAGAG187300.030.406598
GCAGAGT208900.026.989739
GTACATG103150.025.2676561
TACATGG101800.024.7294462
ACATGGG103300.023.8185713
AGAGTAC188650.023.18937710-11
GAGTACT148000.023.17190612-13
CAGAGTA201850.023.0377610-11
AGTACTT154350.021.24923712-13
GTACTTT161400.021.14508814-15
CATGGGG63500.019.448494
ACTTTTT194100.017.83973116-17
Produced by FastQC (version 0.11.5)