FastQCFastQC Report
Fri 10 Feb 2017
SRR2031818_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031818_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405085
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.5546983966328055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.5272967401903303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.2754977350432625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550.2604391670883889No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.16292876803633805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.11775306417171705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.057.736471
GACCCGT250.005019141456.9994057
GTATCAA19050.035.905141
CGCAGAA2000.035.624639
CCGGCGT300.00395047131.66633812-13
AACGCAG21400.031.2964046
ATCAACG21750.031.2295634
CAACGCA21700.031.082635
TCAACGC21700.031.082635
TATCAAC22500.030.1885762
CGCAGAG22750.028.6040978
ACGCAGA23950.027.9642187
GCAGAGT25200.025.6346539
CATGGGG6750.025.3330694
TAGGACA1901.1863449E-824.999744
ACATGGG12000.024.1455843
TACATGG12350.023.8459052
CAGAGTA24350.023.7985210-11
GAGTACT18200.023.74975412-13
GTACATG12800.023.3786641
Produced by FastQC (version 0.11.5)