##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031818_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 405085 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54190355110656 34.0 31.0 34.0 31.0 34.0 2 32.669498994038285 34.0 31.0 34.0 31.0 34.0 3 32.73032820272289 34.0 33.0 34.0 31.0 34.0 4 35.91889356554797 37.0 37.0 37.0 35.0 37.0 5 35.95753730698495 37.0 37.0 37.0 35.0 37.0 6 36.046676623424716 37.0 37.0 37.0 35.0 37.0 7 36.07190836491107 37.0 37.0 37.0 35.0 37.0 8 36.06790673562339 37.0 37.0 37.0 35.0 37.0 9 37.89205722255823 39.0 39.0 39.0 37.0 39.0 10-11 37.9368823827098 39.0 39.0 39.0 37.0 39.0 12-13 37.913779823987554 39.0 39.0 39.0 37.0 39.0 14-15 39.47335374057297 41.0 40.0 41.0 37.0 41.0 16-17 39.413199698828635 41.0 40.0 41.0 37.0 41.0 18-19 39.42935803596776 41.0 40.0 41.0 37.0 41.0 20-21 39.3752150783169 41.0 40.0 41.0 37.0 41.0 22-23 39.27532616611329 41.0 40.0 41.0 36.0 41.0 24-25 39.23521853438167 41.0 40.0 41.0 36.0 41.0 26-27 39.09501832948641 41.0 39.0 41.0 35.5 41.0 28-29 38.96279299406297 41.0 39.0 41.0 35.0 41.0 30-31 38.7907957589148 41.0 39.0 41.0 35.0 41.0 32-33 38.62546502585877 41.0 38.0 41.0 35.0 41.0 34-35 38.42122640927212 40.0 38.0 41.0 35.0 41.0 36-37 38.28390831553871 40.0 38.0 41.0 34.5 41.0 38-39 38.07173679598109 40.0 38.0 41.0 34.0 41.0 40-41 37.9675956897935 40.0 38.0 41.0 34.0 41.0 42-43 37.780376957922414 40.0 38.0 41.0 33.0 41.0 44-45 37.86277324512139 40.0 38.0 41.0 33.0 41.0 46-47 37.67778490934002 40.0 38.0 41.0 33.0 41.0 48-49 37.46288186430009 40.0 38.0 41.0 32.5 41.0 50-51 36.39902859893603 39.0 36.5 40.0 30.5 40.5 52-53 36.40634558179148 39.5 37.0 40.0 30.0 40.5 54-55 37.16548625597097 40.0 37.0 41.0 31.0 41.0 56-57 37.16181418714591 40.0 37.0 41.0 31.0 41.0 58-59 37.046327314020516 40.0 37.0 41.0 31.0 41.0 60-61 36.8728711258131 40.0 36.5 41.0 31.0 41.0 62-63 36.61657676783885 40.0 36.0 41.0 30.0 41.0 64-65 36.31535603638743 39.0 35.0 41.0 29.0 41.0 66-67 36.05596850043818 39.0 35.0 41.0 29.0 41.0 68-69 35.72659071552884 38.5 35.0 41.0 28.5 41.0 70-71 35.36302010689114 37.5 35.0 40.5 28.0 41.0 72-73 34.963826110569386 37.0 35.0 39.5 27.0 41.0 74-75 34.59608353802288 36.5 35.0 39.0 27.0 41.0 76-77 34.20953997309206 36.0 35.0 39.0 26.0 40.5 78-79 33.81340335978869 35.5 35.0 37.5 26.0 39.5 80-81 33.435183973733906 35.0 34.5 37.0 25.5 39.0 82-83 33.12976535788785 35.0 34.0 37.0 25.0 39.0 84-85 32.80265870126023 35.0 34.0 36.0 24.5 37.5 86-87 32.519943962378264 35.0 34.0 36.0 24.0 37.0 88-89 32.29388276534554 35.0 34.0 35.5 23.0 37.0 90-91 32.106676376562945 35.0 34.0 35.0 23.0 36.0 92-93 31.95105965414666 35.0 34.0 35.0 21.0 36.0 94-95 31.76666748953923 35.0 34.0 35.0 20.0 36.0 96-97 31.651237394620882 35.0 34.0 35.0 20.0 36.0 98-99 31.52136588617204 35.0 33.0 35.0 19.0 35.5 100-101 30.069123764148266 34.0 30.0 35.0 14.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1609275547084863 1101 2 0.05518037038395107 1101 3 0.3059294586675989 1101 4 0.07811106340747642 1101 5 0.2062263704984275 1101 6 0.1826150089696128 1101 7 0.22412622223320255 1101 8 0.43048889534136947 1101 9 0.4623003418055376 1101 10-11 0.3761102915861301 1101 12-13 0.2860986321359533 1101 14-15 0.46008148333941534 1101 16-17 0.40014772310814806 1101 18-19 0.31698303172763787 1101 20-21 0.2729340163587963 1101 22-23 0.46830646960128774 1101 24-25 0.3738362031668814 1101 26-27 0.2951630972155712 1101 28-29 0.28422259436314334 1101 30-31 0.1325510621396475 1101 32-33 0.18862891562343265 1101 34-35 0.11176659974961467 1101 36-37 -0.12497109655131311 1101 38-39 0.32964047707232425 1101 40-41 0.21466959781507455 1101 42-43 0.1335667819785442 1101 44-45 0.6033158217783594 1101 46-47 0.7444487963577231 1101 48-49 0.5376028375307413 1101 50-51 0.7254007575635129 1101 52-53 0.812864921956141 1101 54-55 0.6596232559028721 1101 56-57 0.6687340901447314 1101 58-59 0.7067214390174499 1101 60-61 0.6809134497614124 1101 62-63 0.7729899277889558 1101 64-65 0.7301143624451356 1101 66-67 0.49634438733758657 1101 68-69 0.2917099198410753 1101 70-71 0.235244726083927 1101 72-73 0.3871307545580791 1101 74-75 0.48419740430224323 1101 76-77 0.4842821252683862 1101 78-79 0.46221117898209485 1101 80-81 0.4985949000481895 1101 82-83 0.6128697390373716 1101 84-85 0.22722442195408377 1101 86-87 0.30146233906861397 1101 88-89 0.3972774934030596 1101 90-91 0.5072066315832657 1101 92-93 0.5393385085029223 1101 94-95 0.5080637511080575 1101 96-97 0.4011253498632641 1101 98-99 0.16278724910450748 1101 100-101 0.011179928631626979 1102 1 0.02049233681619711 1102 2 0.1288689553591098 1102 3 0.1393320541076335 1102 4 0.09874802292692664 1102 5 0.11217472615305013 1102 6 0.0725732628089375 1102 7 0.018058861686164107 1102 8 0.08443966887317345 1102 9 0.10818522818420462 1102 10-11 0.1782741027650374 1102 12-13 0.3438821187904111 1102 14-15 0.3471685080282043 1102 16-17 0.3692017286617073 1102 18-19 0.423657695019358 1102 20-21 0.5204925270975664 1102 22-23 0.4639618465445565 1102 24-25 0.40058869188030144 1102 26-27 0.5173669544852615 1102 28-29 0.5658061150189937 1102 30-31 0.46376717880222174 1102 32-33 0.4385061327979187 1102 34-35 0.43840669495352813 1102 36-37 0.3416319749082959 1102 38-39 0.4704959900211918 1102 40-41 0.5109067764546396 1102 42-43 0.3717560186471687 1102 44-45 0.3149556336372896 1102 46-47 0.6079567961310488 1102 48-49 0.45673617967925395 1102 50-51 0.5685551427993545 1102 52-53 0.7166693005805911 1102 54-55 0.6257351960604112 1102 56-57 0.7592580894647014 1102 58-59 0.5597078951273176 1102 60-61 0.8040929582523191 1102 62-63 0.8596887698524682 1102 64-65 0.8385363197922757 1102 66-67 0.7848084686387082 1102 68-69 0.764494634323782 1102 70-71 0.5112227385133181 1102 72-73 0.5338184517189646 1102 74-75 0.49309443673580233 1102 76-77 0.6252840331245935 1102 78-79 0.6842072499316743 1102 80-81 0.7124825820973371 1102 82-83 0.6393483276015672 1102 84-85 0.6583338120067808 1102 86-87 0.5506973076173196 1102 88-89 0.5611642309689344 1102 90-91 0.5229464453311721 1102 92-93 0.7334959349748971 1102 94-95 0.5609784264891609 1102 96-97 0.8182799995544201 1102 98-99 0.8112741869008424 1102 100-101 0.6332355020274214 1103 1 -0.15342299042406182 1103 2 -0.07411104954336167 1103 3 0.034156440422719925 1103 4 -0.007145453278560865 1103 5 -0.2778347286216132 1103 6 -0.13177243176682651 1103 7 -0.05686147308289691 1103 8 0.10119563063386039 1103 9 -0.12900705391825795 1103 10-11 0.07435037128063726 1103 12-13 -0.01636230464342958 1103 14-15 7.03543220978986E-4 1103 16-17 -0.06285521009236561 1103 18-19 -0.020524116795613168 1103 20-21 -0.12075130103563936 1103 22-23 0.12606745987929457 1103 24-25 -0.06925232783464708 1103 26-27 -0.15067415627567726 1103 28-29 0.025316917974485875 1103 30-31 -0.021963114996147226 1103 32-33 -0.148836725345447 1103 34-35 -0.17505060098091718 1103 36-37 -0.06328625879972805 1103 38-39 -0.0415938653557717 1103 40-41 -0.06455633241534287 1103 42-43 -0.024950568179178845 1103 44-45 0.09242700668067982 1103 46-47 -0.19899946678172142 1103 48-49 -0.1648802366109834 1103 50-51 -0.22491931180022107 1103 52-53 -0.2063530757194414 1103 54-55 -0.12563049361288137 1103 56-57 -0.185002512139107 1103 58-59 -0.19249821896030284 1103 60-61 -0.26722055507190845 1103 62-63 -0.40426577719379253 1103 64-65 -0.21255448106216335 1103 66-67 0.06887192068199255 1103 68-69 0.06548913654740574 1103 70-71 -0.12493477102899675 1103 72-73 -0.14162014477226137 1103 74-75 0.006865140941421544 1103 76-77 -0.0274368409167991 1103 78-79 0.05537719075179126 1103 80-81 -0.004679584796946301 1103 82-83 -0.09506004862843298 1103 84-85 0.026203336116459752 1103 86-87 0.19699243665748156 1103 88-89 0.08130815364989274 1103 90-91 -0.23274392830751367 1103 92-93 -0.09512751986980206 1103 94-95 -0.09602335941272244 1103 96-97 -0.26111384433674445 1103 98-99 -0.1354814246830074 1103 100-101 -0.02628755711744546 1104 1 0.3366684353234106 1104 2 0.2926709288768663 1104 3 0.2906097803452994 1104 4 0.39856247852277704 1104 5 0.47553814017008733 1104 6 0.35052721406135845 1104 7 0.3796511700272234 1104 8 0.46096830174826664 1104 9 0.37772221055748645 1104 10-11 0.339583877581596 1104 12-13 0.3529400592475227 1104 14-15 0.46818562871641234 1104 16-17 0.5440140757984224 1104 18-19 0.5824204511971445 1104 20-21 0.4805583110226692 1104 22-23 0.3036599151959791 1104 24-25 0.4974555216011396 1104 26-27 0.46934890607503377 1104 28-29 0.6716887160141809 1104 30-31 0.5917548654008513 1104 32-33 0.6188595105494556 1104 34-35 0.2092340543193103 1104 36-37 0.25831410360864737 1104 38-39 0.29678847547282317 1104 40-41 0.13626732296724953 1104 42-43 0.11800717829969187 1104 44-45 0.3960469720871558 1104 46-47 0.31779486646924937 1104 48-49 0.4090428339762866 1104 50-51 0.6709377016253697 1104 52-53 0.5854556715007249 1104 54-55 0.7046759061894434 1104 56-57 0.6155328638595776 1104 58-59 0.4949739826705368 1104 60-61 0.7191726580077358 1104 62-63 0.5275685033432325 1104 64-65 0.5910243015751817 1104 66-67 0.5651407617988653 1104 68-69 0.3217983367780235 1104 70-71 0.4941863886777824 1104 72-73 0.4507198601696629 1104 74-75 0.46189767532903403 1104 76-77 0.5769244143340089 1104 78-79 0.6316517974563425 1104 80-81 0.8073210741273584 1104 82-83 0.7754324972903603 1104 84-85 0.5958475169911779 1104 86-87 0.7106239332393116 1104 88-89 0.7925634044529559 1104 90-91 0.7483449441082612 1104 92-93 0.5753361981105911 1104 94-95 0.6664056003105294 1104 96-97 0.7546509196859859 1104 98-99 0.7314858761983096 1104 100-101 0.49355430104909814 1105 1 -0.0015727766543420785 1105 2 -0.10209768279315767 1105 3 -0.02604307798113581 1105 4 0.02978510841980153 1105 5 0.08667949812171827 1105 6 0.02902171045146673 1105 7 0.010242561513400972 1105 8 0.11623322461882424 1105 9 0.07080620471852939 1105 10-11 0.1922922848881896 1105 12-13 0.2505058461256553 1105 14-15 0.3747268367489198 1105 16-17 0.46161773698120356 1105 18-19 0.3960196937110467 1105 20-21 0.3916325998931498 1105 22-23 0.39555244685654145 1105 24-25 0.2607884604104669 1105 26-27 0.3484904218362672 1105 28-29 0.23247697497974684 1105 30-31 0.06014165673710892 1105 32-33 0.1189897034716978 1105 34-35 -0.005730241258078195 1105 36-37 -0.20969394468109925 1105 38-39 -0.11864924891270334 1105 40-41 -0.2340486907621866 1105 42-43 -0.20772367742287656 1105 44-45 0.030323217796706103 1105 46-47 0.08519140254846747 1105 48-49 -0.41813592485118534 1105 50-51 -0.1782831134612337 1105 52-53 -0.229757174200941 1105 54-55 -0.21626148285067615 1105 56-57 -0.19279159268360502 1105 58-59 -0.47998163337869215 1105 60-61 -0.3821647783752695 1105 62-63 -0.22111181385406553 1105 64-65 -0.30217804978789076 1105 66-67 -0.3824033262588955 1105 68-69 -0.3088167261485495 1105 70-71 -0.3131872650090415 1105 72-73 -0.32612552587207233 1105 74-75 -0.3073886307426861 1105 76-77 -0.2538484581952858 1105 78-79 -0.09498646348183826 1105 80-81 -0.126872321540759 1105 82-83 -0.31088146491423885 1105 84-85 -0.33215579701047915 1105 86-87 -0.07631337689600315 1105 88-89 -0.3464155674174805 1105 90-91 -0.2759892966302857 1105 92-93 -0.5171875719608146 1105 94-95 -0.2520383970067037 1105 96-97 -0.12710234500016782 1105 98-99 -0.2406094408017374 1105 100-101 -0.07538087865658838 1106 1 0.14496222992123364 1106 2 0.29199098715758254 1106 3 0.28133716854718926 1106 4 0.2203430292008477 1106 5 0.19954904912514593 1106 6 0.22439428919118853 1106 7 0.27443205694021344 1106 8 0.10082334713997909 1106 9 0.2523663619379164 1106 10-11 0.29074015209872783 1106 12-13 0.3999980037314046 1106 14-15 0.3792925887791725 1106 16-17 0.5332171990472006 1106 18-19 0.3914502266669402 1106 20-21 0.4892817391244435 1106 22-23 0.311251352413457 1106 24-25 0.29217289747442265 1106 26-27 0.18656039783728318 1106 28-29 0.20011177425754312 1106 30-31 0.14044168125619905 1106 32-33 0.15337215671824822 1106 34-35 0.4713964184243622 1106 36-37 0.34221965895664397 1106 38-39 0.5012342482527359 1106 40-41 0.6250445486556018 1106 42-43 0.4345985034388633 1106 44-45 0.5520226137354527 1106 46-47 0.5447609067427166 1106 48-49 0.6283240384444753 1106 50-51 0.6248100070761069 1106 52-53 0.7556391063271874 1106 54-55 0.6489169492648799 1106 56-57 0.8892990685145605 1106 58-59 0.6961657486510333 1106 60-61 0.6543129426866372 1106 62-63 0.861606684685924 1106 64-65 1.1437945036897261 1106 66-67 1.0134016800937786 1106 68-69 1.0222615937735853 1106 70-71 0.8494746167197746 1106 72-73 0.7327574437614075 1106 74-75 0.6171037126137549 1106 76-77 0.4179189224970443 1106 78-79 0.4565630687979194 1106 80-81 0.3853649341042029 1106 82-83 0.46785973979044826 1106 84-85 0.5818713523107917 1106 86-87 0.6368764083018874 1106 88-89 0.8037824428210953 1106 90-91 0.5611816460173316 1106 92-93 0.7892862111863934 1106 94-95 0.6260088431094992 1106 96-97 0.5579796453556582 1106 98-99 0.3271932771023991 1106 100-101 0.47841252199279793 1107 1 0.19718498732081713 1107 2 0.20882668695474393 1107 3 0.2552868452925239 1107 4 0.18689142920899116 1107 5 0.3635032711091739 1107 6 0.24312474296882414 1107 7 0.2394472868818056 1107 8 0.17128133081782693 1107 9 0.21853486139859513 1107 10-11 0.1707967301445521 1107 12-13 0.20398288911676588 1107 14-15 0.1585534108568183 1107 16-17 0.2537867785108574 1107 18-19 0.2644888204921969 1107 20-21 0.3285464958863358 1107 22-23 0.34842055061977817 1107 24-25 0.36875338551094217 1107 26-27 0.20557238505634245 1107 28-29 0.1732880820312559 1107 30-31 0.34457717007287414 1107 32-33 0.3179301492152078 1107 34-35 0.5586955290500413 1107 36-37 0.42220229207447346 1107 38-39 0.35790659081448695 1107 40-41 0.41721181343949354 1107 42-43 0.37850211724281735 1107 44-45 0.2767609233136952 1107 46-47 0.3824755335921921 1107 48-49 0.6252814420955914 1107 50-51 0.4967869145499719 1107 52-53 0.2579422533961875 1107 54-55 0.26364352262977064 1107 56-57 0.3065994680885282 1107 58-59 0.07153462529868904 1107 60-61 0.24041468819397238 1107 62-63 0.14132881457121016 1107 64-65 0.05721788249805826 1107 66-67 -0.003704020449610823 1107 68-69 0.11890218750386339 1107 70-71 0.034638821947474696 1107 72-73 0.19750131616908817 1107 74-75 0.3687165939558881 1107 76-77 0.48984902079630643 1107 78-79 0.404343703367509 1107 80-81 0.2660761622005623 1107 82-83 0.2814337220301013 1107 84-85 0.38651427971586116 1107 86-87 0.33886138866238724 1107 88-89 0.5263770278815016 1107 90-91 0.4277008750905722 1107 92-93 0.16214503064544772 1107 94-95 0.09883238608914269 1107 96-97 0.246603659140618 1107 98-99 0.4087633517935636 1107 100-101 0.5537142376508051 1108 1 0.0425191877597868 1108 2 -0.020714518280406935 1108 3 -0.0424388783550711 1108 4 -0.07978732691129409 1108 5 0.012925791260002484 1108 6 0.16472880502524134 1108 7 0.018454159110874002 1108 8 0.008886618494713616 1108 9 0.1934596670814699 1108 10-11 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46-47 -0.5024460914735371 2316 48-49 -0.5237245017453631 2316 50-51 -0.41323563105363803 2316 52-53 -0.5485041852004642 2316 54-55 -0.5746341001902948 2316 56-57 -0.47003419704242333 2316 58-59 -0.6312933775885767 2316 60-61 -0.659629422769612 2316 62-63 -0.9158750850161965 2316 64-65 -0.7288706780587191 2316 66-67 -0.43994585546570164 2316 68-69 -0.314418967044908 2316 70-71 -0.32845678745216844 2316 72-73 -0.46269722772354527 2316 74-75 -0.9018752058605415 2316 76-77 -0.9007023216919023 2316 78-79 -0.32937534098405763 2316 80-81 -0.21275057528128372 2316 82-83 -0.38596515692763234 2316 84-85 -0.3672039626181842 2316 86-87 -0.3552469015253692 2316 88-89 -0.4263329002042582 2316 90-91 -0.5325992576878775 2316 92-93 -0.47064456451393255 2316 94-95 -0.5868235305723282 2316 96-97 -0.3656587431691918 2316 98-99 -0.5244620062181475 2316 100-101 -0.14848329035297425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 253.0 8 449.0 9 370.0 10 370.0 11 449.0 12 520.0 13 496.0 14 528.0 15 582.0 16 779.0 17 1208.0 18 1596.0 19 2225.0 20 2946.0 21 3469.0 22 3821.0 23 3803.0 24 3180.0 25 2923.0 26 2688.0 27 2590.0 28 2909.0 29 3265.0 30 3910.0 31 4551.0 32 5850.0 33 7652.0 34 11115.0 35 19711.0 36 34187.0 37 78498.0 38 142219.0 39 55876.0 40 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29186467037782 20.828468099287804 14.657911302566129 22.221755927768246 2 15.041287630991027 22.291864670377823 40.72009578236667 21.946751916264486 3 17.085796808077315 27.475221249861143 32.70696273621586 22.732019205845685 4 11.813076267943764 19.332238912820763 40.17996223014923 28.674722589086244 5 11.906390016910033 38.82765345544762 36.42025747682585 12.845699050816496 6 27.47299949393337 39.64427218978733 18.973055037831564 13.909673278447734 7 23.883876223508647 35.2395176321019 24.255896910524953 16.6207092338645 8 23.73205623511115 35.77742942839157 22.359998518829382 18.1305158176679 9 25.124356616512582 16.13044175913697 22.694989940382882 36.05021168396757 10-11 22.43837713072565 27.30068997864646 30.87166890899441 19.389263981633484 12-13 23.716874236271398 26.188947998568203 30.05937025562536 20.034807509535035 14-15 21.37107026920276 27.811446980263398 28.2493797598035 22.568102990730342 16-17 19.743880913882272 30.669982843107 29.053285113000975 20.532851130009753 18-19 19.543552587728502 29.47393756865843 31.58843205746942 19.394077786143647 20-21 20.521125195946528 28.582025994544352 31.80147376476542 19.095375044743694 22-23 20.37352700485948 28.74684170833493 31.238173763745593 19.641457523060005 24-25 20.246047733309634 28.756257961311725 31.234756248086814 19.76293805729182 26-27 19.864719750175887 28.975153362874455 31.298122616241038 19.862004270708617 28-29 19.980868212844218 28.746682794968958 31.459817075428614 19.81263191675821 30-31 20.294259229544416 28.715207919325575 31.393781552019945 19.596751299110064 32-33 19.856449880889198 29.045508967253785 31.22468123973981 19.873359912117213 34-35 20.196501968722615 29.056617746892627 31.030771319599594 19.716108964785168 36-37 20.352518607205894 28.99983953984966 30.72392213979782 19.923719713146625 38-39 20.18082624634336 29.090067516694027 30.89573793154523 19.83336830541738 40-41 20.201686065887404 28.927879333966942 31.062739918782476 19.80769468136317 42-43 20.12725724230717 29.175851981682854 30.608020538899243 20.08887023711073 44-45 20.30833035042028 29.23732056235111 30.367206882506142 20.087142204722465 46-47 20.349062542429365 29.093647012355433 30.17798733599121 20.37930310922399 48-49 20.4049767332782 29.122653270301296 30.073935100040732 20.398434896379772 50-51 20.51248503400521 29.270646901267632 29.76214868484392 20.454719379883237 52-53 20.185269758198896 29.40987693940778 29.820901786044907 20.58395151634842 54-55 20.24550403001839 29.302615500450525 29.93458163101571 20.517298838515373 56-57 20.398928619919275 29.358899983953986 29.705247046916078 20.536924349210658 58-59 20.15391831344039 29.291877013466312 29.913474949701914 20.640729723391388 60-61 20.33042447881309 29.137958700025923 29.860769961859855 20.670846859301133 62-63 20.05825937766148 29.220287100238224 30.04480541120999 20.67664811089031 64-65 20.12022168186924 28.945653381389093 30.11047064196403 20.82365429477764 66-67 20.141081501413282 29.146722292852118 29.800041966500856 20.91215423923374 68-69 20.139847192564524 29.073034054581136 29.725613142920622 21.061505609933718 70-71 20.15984299591444 28.89368897885629 29.71610896478517 21.230359060444105 72-73 20.14861078539072 28.8555488354296 29.52158189022057 21.47425848895911 74-75 20.08640161941321 28.99675376772776 29.42814471036943 21.4886999024896 76-77 20.03159830652826 28.903440018761493 29.450485700531985 21.61447597417826 78-79 19.933223891282076 28.777663947072835 29.49590826616636 21.793203895478726 80-81 20.090351407729244 28.778651394151844 29.284347729488875 21.84664946863004 82-83 20.12083883629362 28.536603428909984 29.470728365651656 21.871829369144745 84-85 20.01826777096165 28.41243195872471 29.307305874075812 22.261994396237828 86-87 19.882740659367787 28.492785464778997 29.522569337299583 22.101904538553637 88-89 20.06406062925065 28.263450880679365 29.46085389486157 22.211634595208412 90-91 20.123060592221385 28.139526272263847 29.384943900662826 22.352469234851945 92-93 20.086031326758583 28.08978362565881 29.514669760667516 22.30951528691509 94-95 19.919029339521334 27.93685275929743 29.662786822518733 22.481331078662503 96-97 19.95976153153042 27.816631077428195 29.581939592924943 22.641667798116444 98-99 20.02443931520545 27.723070466692175 29.423454336744143 22.829035881358234 100-101 20.29388893688979 27.488058061888243 29.545897774541146 22.67215522668082 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1297.0 1 790.0 2 316.5 3 362.0 4 279.5 5 224.0 6 356.0 7 637.0 8 964.5 9 1055.5 10 817.0 11 602.5 12 613.0 13 641.0 14 647.0 15 676.0 16 720.5 17 746.5 18 812.5 19 957.5 20 1136.0 21 1333.0 22 1554.5 23 1879.0 24 2314.5 25 2799.0 26 3479.5 27 4265.5 28 4919.5 29 5826.5 30 7012.5 31 7923.5 32 8915.0 33 10285.5 34 11308.5 35 12047.5 36 13114.0 37 14149.5 38 14727.0 39 15513.5 40 15977.5 41 15745.0 42 15425.5 43 15637.5 44 16012.5 45 15773.0 46 15208.5 47 14703.5 48 14350.5 49 13643.0 50 12655.0 51 11391.5 52 10223.5 53 9463.5 54 8680.0 55 7659.0 56 6557.5 57 5589.5 58 4762.0 59 3990.5 60 3286.0 61 2663.5 62 2152.0 63 1609.0 64 1094.0 65 805.0 66 604.0 67 442.5 68 331.0 69 266.5 70 200.5 71 158.5 72 153.0 73 125.0 74 92.0 75 73.0 76 48.5 77 29.5 78 26.5 79 21.0 80 17.5 81 15.5 82 10.0 83 7.0 84 6.5 85 5.5 86 6.0 87 5.5 88 2.5 89 2.0 90 1.5 91 0.5 92 2.0 93 2.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.2343088487601367E-4 24-25 2.4686176975202733E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 405085.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.24438203290134 #Duplication Level Percentage of deduplicated Percentage of total 1 72.35215127171715 44.31162793387314 2 15.964201465823328 19.55435306846175 3 5.70805993068921 10.48759809185479 4 2.483393143677657 6.08375513717129 5 1.305297974220991 3.9971083899981297 6 0.6984011197703411 2.566388699485252 7 0.42503686077957376 1.8221783915754524 8 0.26543295458218147 1.30050218196423 9 0.18312590644477542 1.0093889676982657 >10 0.5900171453526399 5.6681653476799285 >50 0.018355741188986023 0.7754385386548457 >100 0.004486958955294467 0.6213001433549942 >500 4.079053595722243E-4 0.16488061705440135 >1k 0.0016316214382888971 1.637314491173555 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2247 0.5546983966328055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2136 0.5272967401903303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1116 0.2754977350432625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1055 0.2604391670883889 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 660 0.16292876803633805 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.11775306417171705 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.4686176975202733E-4 0.0 10-11 0.0 0.0 0.0 2.4686176975202733E-4 0.0 12-13 0.0 0.0 0.0 2.4686176975202733E-4 0.0 14-15 0.0 0.0 0.0 3.70292654628041E-4 0.0 16-17 0.0 0.0 0.0 8.640161941320957E-4 0.0 18-19 0.0 0.0 0.0 9.874470790081093E-4 0.0 20-21 0.0 0.0 0.0 0.0013577397336361503 0.0 22-23 0.0 0.0 0.0 0.003826357431156424 0.0 24-25 0.0 0.0 0.0 0.008269869286692915 0.0 26-27 0.0 0.0 0.0 0.014935137069997655 0.0 28-29 0.0 0.0 0.0 0.032338891837515586 0.0 30-31 0.0 0.0 0.0 0.057148499697594335 0.0 32-33 0.0 0.0 0.0 0.09035140772924201 0.0 34-35 0.0 0.0 0.0 0.14614216769320018 0.0 36-37 0.0 0.0 0.0 0.22464421047434488 0.0 38-39 0.0 0.0 0.0 0.3393115025241616 0.0 40-41 0.0 0.0 0.0 0.46533443598257157 0.0 42-43 0.0 0.0 0.0 0.5721021514003234 0.0 44-45 0.0 0.0 0.0 0.6899786464569164 0.0 46-47 0.0 0.0 0.0 0.8429095128182974 0.0 48-49 0.0 0.0 0.0 1.0054679882000075 0.0 50-51 0.0 0.0 0.0 1.1565473912882482 0.0 52-53 0.0 0.0 0.0 1.3131811841959093 0.0 54-55 0.0 0.0 0.0 1.4924028290358815 0.0 56-57 0.0 0.0 0.0 1.7292666971129518 0.0 58-59 0.0 0.0 0.0 1.984398336151672 0.0 60-61 0.0 0.0 0.0 2.2410111458089044 0.0 62-63 0.0 0.0 0.0 2.481454509547379 0.0 64-65 0.0 0.0 0.0 2.7460903267215526 0.0 66-67 0.0 0.0 0.0 3.068738659787452 0.0 68-69 0.0 0.0 0.0 3.4391547453003692 0.0 70-71 0.0 0.0 0.0 3.8483281286643543 0.0 72-73 0.0 0.0 0.0 4.255896910524951 0.0 74-75 0.0 0.0 0.0 4.671982423441994 0.0 76-77 0.0 0.0 0.0 5.126825234210104 0.0 78-79 0.0 0.0 0.0 5.65054247873903 0.0 80-81 0.0 0.0 0.0 6.21437476085266 0.0 82-83 0.0 0.0 0.0 6.805114975869262 0.0 84-85 0.0 0.0 0.0 7.4072108322944565 0.0 86-87 0.0 1.2343088487601367E-4 0.0 8.081266894602368 0.0 88-89 0.0 2.4686176975202733E-4 0.0 8.794080254761347 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1160 0.0 57.73647 1 GACCCGT 25 0.0050191414 56.999405 7 GTATCAA 1905 0.0 35.90514 1 CGCAGAA 200 0.0 35.62463 9 CCGGCGT 30 0.003950471 31.666338 12-13 AACGCAG 2140 0.0 31.296404 6 ATCAACG 2175 0.0 31.229563 4 CAACGCA 2170 0.0 31.08263 5 TCAACGC 2170 0.0 31.08263 5 TATCAAC 2250 0.0 30.188576 2 CGCAGAG 2275 0.0 28.604097 8 ACGCAGA 2395 0.0 27.964218 7 GCAGAGT 2520 0.0 25.634653 9 CATGGGG 675 0.0 25.333069 4 TAGGACA 190 1.1863449E-8 24.99974 4 ACATGGG 1200 0.0 24.145584 3 TACATGG 1235 0.0 23.845905 2 CAGAGTA 2435 0.0 23.79852 10-11 GAGTACT 1820 0.0 23.749754 12-13 GTACATG 1280 0.0 23.378664 1 >>END_MODULE