FastQCFastQC Report
Fri 10 Feb 2017
SRR2031818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405085
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27480.6783761432785712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.6275226187096535No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16940.41818383795993436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.32832615377019636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8980.22168186923732058No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.21649777207252802No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.16490366219435426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.10960662576990014No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT4060.1002258785193231RNA PCR Primer, Index 39 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCC200.00207344671.247046
GGTATCA11150.058.0422821
GAACCGG459.6196495E-442.2204676
CGCAGAA1950.038.972749
GTATCAA17200.037.832732
CTACGCT600.00394876131.6653524
TCAACGC21650.029.3983175
ATCAACG22100.029.0146314
CAACGCA22150.028.73476
AACGCAG22550.028.4356257
TATCAAC22600.028.3727153
ACGCAGA24700.025.575868
CGCAGAG23850.025.0932948
CATGGGG4600.022.7164484
GGACAGT2103.4337063E-822.6181096
GCAGAGT26650.022.1003959
ACTACTA1302.9377834E-421.9221655
CCTATTC1302.9377834E-421.9221653
GTATACA1100.002844117321.6300621
GTAGCAC1100.00287487821.5900123
Produced by FastQC (version 0.11.5)