##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031818_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 405085 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8670945604009 34.0 33.0 34.0 31.0 34.0 2 32.997548662626365 34.0 33.0 34.0 31.0 34.0 3 33.03809323969043 34.0 33.0 34.0 31.0 34.0 4 36.41344409198069 37.0 37.0 37.0 35.0 37.0 5 36.41033857091721 37.0 37.0 37.0 35.0 37.0 6 36.48499944456102 37.0 37.0 37.0 35.0 37.0 7 36.4362417764173 37.0 37.0 37.0 35.0 37.0 8 36.48087191577076 37.0 37.0 37.0 35.0 37.0 9 38.28311095202241 39.0 39.0 39.0 37.0 39.0 10-11 38.30321660885987 39.0 39.0 39.0 37.0 39.0 12-13 38.31940086648481 39.0 39.0 39.0 37.0 39.0 14-15 39.87561869731044 41.0 40.0 41.0 38.0 41.0 16-17 39.84979695619438 41.0 40.0 41.0 38.0 41.0 18-19 39.89215473295728 41.0 40.0 41.0 38.0 41.0 20-21 39.895294814668524 41.0 40.0 41.0 38.0 41.0 22-23 39.84074947233297 41.0 40.0 41.0 37.5 41.0 24-25 39.76523569127467 41.0 40.0 41.0 37.0 41.0 26-27 39.6353407309577 41.0 40.0 41.0 37.0 41.0 28-29 39.57590382265451 41.0 40.0 41.0 36.0 41.0 30-31 39.412776330893514 41.0 39.0 41.0 36.0 41.0 32-33 38.93655529086488 40.0 38.5 41.0 35.0 41.0 34-35 38.95233469518743 40.0 39.0 41.0 35.0 41.0 36-37 38.883445449720426 41.0 39.0 41.0 35.0 41.0 38-39 38.76748830492366 41.0 38.0 41.0 35.0 41.0 40-41 38.649124257871804 40.5 38.0 41.0 35.0 41.0 42-43 38.48663984102102 40.0 38.0 41.0 35.0 41.0 44-45 38.22606366565042 40.0 38.0 41.0 34.5 41.0 46-47 37.97920313020724 40.0 38.0 41.0 33.5 41.0 48-49 37.782574027673206 40.0 38.0 41.0 33.0 41.0 50-51 37.753435698680526 40.0 38.0 41.0 33.0 41.0 52-53 37.817084068775685 41.0 38.0 41.0 33.0 41.0 54-55 37.74637421775677 41.0 38.0 41.0 33.0 41.0 56-57 37.60601231840231 40.5 38.0 41.0 33.0 41.0 58-59 37.43838700519644 40.0 37.0 41.0 33.0 41.0 60-61 37.22526383351642 40.0 37.0 41.0 32.5 41.0 62-63 36.97623955466136 40.0 36.0 41.0 32.0 41.0 64-65 36.70943875976647 39.0 36.0 41.0 31.0 41.0 66-67 36.43592579335201 39.0 35.0 41.0 31.0 41.0 68-69 36.039347297480774 39.0 35.0 41.0 30.5 41.0 70-71 35.65293086636138 37.5 35.0 40.0 30.0 41.0 72-73 35.244748633002956 37.0 35.0 39.5 29.0 41.0 74-75 34.831144080871915 36.5 35.0 39.0 29.0 41.0 76-77 33.35533653430761 35.0 33.0 37.5 26.5 39.0 78-79 34.00655911722231 36.0 35.0 37.5 28.0 39.0 80-81 33.813704531147785 35.0 35.0 37.0 28.0 39.0 82-83 33.49095128182974 35.0 35.0 37.0 27.5 39.0 84-85 33.209723885110535 35.0 35.0 36.0 27.0 37.5 86-87 32.96014910450893 35.0 34.0 36.0 27.0 37.0 88-89 32.7629250651098 35.0 34.0 36.0 26.0 37.0 90-91 32.56142784847624 35.0 34.0 35.0 25.5 36.0 92-93 32.4156818939235 35.0 34.0 35.0 25.0 36.0 94-95 32.32959008603133 35.0 34.0 35.0 25.0 36.0 96-97 32.237579767209354 35.0 34.0 35.0 25.0 36.0 98-99 32.172997025315674 35.0 34.0 35.0 25.0 36.0 100-101 31.10618882456768 34.5 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39768209815467515 1101 2 0.36711642227200514 1101 3 0.34546407546048385 1101 4 0.23640973599170678 1101 5 0.09320465899514829 1101 6 0.06799010571680242 1101 7 0.07841549471913822 1101 8 0.2337457829261922 1101 9 0.2258868382646142 1101 10-11 0.27750522227480445 1101 12-13 0.19772517734290318 1101 14-15 0.27582517551516617 1101 16-17 0.2303970321735207 1101 18-19 0.3084072380059055 1101 20-21 0.41435926047598315 1101 22-23 0.35301966592118816 1101 24-25 0.3427261207934933 1101 26-27 0.4007947176679352 1101 28-29 0.3028520066861162 1101 30-31 0.22839644468361797 1101 32-33 0.26095628468600296 1101 34-35 0.3369924001719795 1101 36-37 0.5342239635705894 1101 38-39 0.5591888543509214 1101 40-41 0.31463371901278947 1101 42-43 0.42728291499432913 1101 44-45 0.6756908113260778 1101 46-47 0.5121791815152648 1101 48-49 0.7435776006092496 1101 50-51 0.9301849822216113 1101 52-53 0.7562204346537342 1101 54-55 0.809831572381519 1101 56-57 0.7743316219647127 1101 58-59 0.7319740805681292 1101 60-61 0.4311384828876754 1101 62-63 0.47244253488602794 1101 64-65 0.6738304052697188 1101 66-67 0.711956458895358 1101 68-69 0.6352997442849144 1101 70-71 0.505044361237097 1101 72-73 0.4309374871290217 1101 74-75 0.5051495212470982 1101 76-77 0.5272073840331544 1101 78-79 0.30964698406724267 1101 80-81 0.44454033110704216 1101 82-83 0.5493544258632568 1101 84-85 0.47248601780366073 1101 86-87 0.6340739160087097 1101 88-89 0.544487901179437 1101 90-91 0.5480727283412321 1101 92-93 0.7339440209555477 1101 94-95 0.4308247240430845 1101 96-97 0.4089169722039543 1101 98-99 0.46586445111601904 1101 100-101 0.5640445099450595 1102 1 0.4801638224033411 1102 2 0.3817464681738869 1102 3 0.39286769094245244 1102 4 0.2580289581152826 1102 5 0.2358216332485341 1102 6 0.25594227708384665 1102 7 0.2999440464484877 1102 8 0.20296507305256029 1102 9 0.25991657059805817 1102 10-11 0.12424392153243247 1102 12-13 0.24418430955166315 1102 14-15 0.423967376916778 1102 16-17 0.37220647322831724 1102 18-19 0.336170323821662 1102 20-21 0.22125493285362552 1102 22-23 0.18924626634809982 1102 24-25 0.28548571202113493 1102 26-27 0.4037320606475916 1102 28-29 0.37960543988281614 1102 30-31 0.34349589877228937 1102 32-33 0.30299523517619065 1102 34-35 0.19938590968293113 1102 36-37 0.36065627245439913 1102 38-39 0.09812045487227294 1102 40-41 0.12400865998258581 1102 42-43 0.060360608188382514 1102 44-45 0.24415640380043868 1102 46-47 0.43486850318737424 1102 48-49 0.4801281587988697 1102 50-51 0.6136083562936605 1102 52-53 0.7905098998873115 1102 54-55 0.5453101420560458 1102 56-57 0.5815436962388816 1102 58-59 0.6518205075878996 1102 60-61 0.7310638687090929 1102 62-63 0.6630439818344058 1102 64-65 0.394348359896739 1102 66-67 0.30476138532011277 1102 68-69 0.2996114993237313 1102 70-71 0.35036161316856607 1102 72-73 0.5054572173845955 1102 74-75 0.3607238615255355 1102 76-77 0.48719435016398194 1102 78-79 0.43569772170868504 1102 80-81 0.4391520540334284 1102 82-83 0.2736880929140142 1102 84-85 0.3070833845843026 1102 86-87 0.5176474629211967 1102 88-89 0.5143163830170252 1102 90-91 0.45252407800782635 1102 92-93 0.4740694483342338 1102 94-95 0.5969498869664491 1102 96-97 0.4208032941971851 1102 98-99 0.20930501478363794 1102 100-101 0.3936691722922845 1103 1 0.4107062463274218 1103 2 0.2940446787702413 1103 3 0.21660617887886247 1103 4 0.1461999844836015 1103 5 0.02214372590532321 1103 6 -0.012608340978275123 1103 7 0.1420142919224574 1103 8 0.17801497325216076 1103 9 0.05425771287490733 1103 10-11 0.06263906107790262 1103 12-13 0.07722874699257432 1103 14-15 0.20358358310090807 1103 16-17 0.15757433399800647 1103 18-19 0.1988134586047181 1103 20-21 0.1538809089715656 1103 22-23 0.08710843674414548 1103 24-25 -0.005563767966258126 1103 26-27 0.05284611324415067 1103 28-29 0.22508990953999586 1103 30-31 0.33074946182162535 1103 32-33 0.4639190016782706 1103 34-35 0.3083521876533055 1103 36-37 0.1592170456448585 1103 38-39 0.21976313442544892 1103 40-41 0.45709686952840656 1103 42-43 0.41304121189325116 1103 44-45 0.4242611066753881 1103 46-47 0.3879885788255706 1103 48-49 0.5390191487487854 1103 50-51 0.79500871347404 1103 52-53 0.5669044791498337 1103 54-55 0.5547999344011814 1103 56-57 0.6540565652377239 1103 58-59 0.52931114035745 1103 60-61 0.3495899743924653 1103 62-63 0.4979881286285348 1103 64-65 0.6363905912235595 1103 66-67 0.687347091101536 1103 68-69 0.28493908975689664 1103 70-71 0.20342741139667453 1103 72-73 0.10762131025149557 1103 74-75 0.3708264633394478 1103 76-77 -0.07979447919483817 1103 78-79 -0.00226845120864283 1103 80-81 0.21337553689227207 1103 82-83 0.256730779470729 1103 84-85 0.2728623529633296 1103 86-87 0.31971875892568136 1103 88-89 0.21202622648797842 1103 90-91 0.22044437775920045 1103 92-93 0.2475400509885688 1103 94-95 0.2791468964562469 1103 96-97 0.0903326565251632 1103 98-99 0.207991851636244 1103 100-101 -0.1713586667664373 1104 1 0.22881019163670402 1104 2 0.230030182435506 1104 3 0.2840965009281149 1104 4 0.07839667338896561 1104 5 0.10249954503060366 1104 6 0.007093495773226266 1104 7 0.08647520947454979 1104 8 -0.00696155256943598 1104 9 0.22222575504606112 1104 10-11 0.17531923569284658 1104 12-13 0.0665171087420191 1104 14-15 0.08530667016128035 1104 16-17 -0.03201466196608038 1104 18-19 -0.07844129800511723 1104 20-21 0.0712971020363824 1104 22-23 -0.00971637175597806 1104 24-25 0.004035935517805456 1104 26-27 -0.04505768380716546 1104 28-29 -0.16853911607568506 1104 30-31 -0.026062168192382273 1104 32-33 0.13495104187266094 1104 34-35 -0.10128458032297516 1104 36-37 -0.2603777159069054 1104 38-39 -0.09795781113093227 1104 40-41 -0.0153436213701994 1104 42-43 -0.20867034399176276 1104 44-45 -0.04105064688691584 1104 46-47 -0.25041023511067806 1104 48-49 -0.0823876546446698 1104 50-51 0.1513961609660086 1104 52-53 0.38243450138853063 1104 54-55 0.39818186340209394 1104 56-57 0.23094067692539255 1104 58-59 0.360671819049486 1104 60-61 0.36935770357457187 1104 62-63 0.49599076432576084 1104 64-65 0.6388368477098467 1104 66-67 0.6296665046588998 1104 68-69 0.8970138776656356 1104 70-71 0.7076568817862636 1104 72-73 0.6817750043458872 1104 74-75 0.8197227651267838 1104 76-77 0.46708386690514914 1104 78-79 0.807714252028326 1104 80-81 0.6889665096292745 1104 82-83 0.5426009713824342 1104 84-85 0.6252962546188741 1104 86-87 0.5007376206339416 1104 88-89 0.3135952899918024 1104 90-91 0.29062393463259895 1104 92-93 0.28219123293403925 1104 94-95 0.35699880321845257 1104 96-97 0.5011060689294169 1104 98-99 0.45422362826090534 1104 100-101 0.37217975675716275 1105 1 0.259101744877718 1105 2 0.2007722820922737 1105 3 0.38604939476170586 1105 4 0.1429565818593943 1105 5 0.1977297497885644 1105 6 0.1668599375612132 1105 7 0.1338812141299357 1105 8 0.13319704608129257 1105 9 0.2418099267429099 1105 10-11 0.263570310770767 1105 12-13 0.20995025664907274 1105 14-15 0.23680388876702807 1105 16-17 0.21961669480197799 1105 18-19 0.2628338035848259 1105 20-21 0.07140756875828203 1105 22-23 0.24459774825822223 1105 24-25 0.18450699718190577 1105 26-27 0.2456197139124825 1105 28-29 0.14416455694281893 1105 30-31 0.14942605526601938 1105 32-33 0.4454414352130911 1105 34-35 0.24267328451310277 1105 36-37 0.3426167213045943 1105 38-39 0.4473295996080182 1105 40-41 0.2682915228283207 1105 42-43 0.18192420368645656 1105 44-45 0.18330332005196937 1105 46-47 0.19353184876513296 1105 48-49 0.28605831529259973 1105 50-51 0.3854554184718282 1105 52-53 0.40329693086736285 1105 54-55 0.48671305052483405 1105 56-57 0.5678483977602085 1105 58-59 0.3350632657690156 1105 60-61 0.23835600712134664 1105 62-63 0.41888104719946995 1105 64-65 0.3677041692863696 1105 66-67 0.2888017080360257 1105 68-69 0.4097609483248874 1105 70-71 0.5458152437225863 1105 72-73 0.5827060810019091 1105 74-75 0.7110274560172272 1105 76-77 0.4931697942338289 1105 78-79 0.5046606362157959 1105 80-81 0.5781723328036179 1105 82-83 0.44284557626663457 1105 84-85 0.46790879259181395 1105 86-87 0.3508382242556749 1105 88-89 0.31585196731062837 1105 90-91 0.19603285876563348 1105 92-93 0.29624023281569123 1105 94-95 0.24091405946179378 1105 96-97 0.3413277914212358 1105 98-99 0.2445538448545861 1105 100-101 0.06540972480071616 1106 1 0.3135712780490749 1106 2 0.37089019663208944 1106 3 0.4177013128233611 1106 4 0.2044623512759145 1106 5 0.24565342854388206 1106 6 0.24688876606874288 1106 7 0.28850009085834216 1106 8 0.19039339942369082 1106 9 0.31216485916680625 1106 10-11 0.21925019545673052 1106 12-13 0.16668149025657186 1106 14-15 0.278753825240301 1106 16-17 0.37954061131818406 1106 18-19 0.35839510502847816 1106 20-21 0.23292522621915168 1106 22-23 0.2600224857924971 1106 24-25 0.39451301550435147 1106 26-27 0.3050487905829442 1106 28-29 0.31285962117539867 1106 30-31 0.39473956528541265 1106 32-33 0.5428973937238126 1106 34-35 0.23484946533025663 1106 36-37 0.19309484358799267 1106 38-39 0.1907521675741961 1106 40-41 0.4033174164749056 1106 42-43 0.1848702096750614 1106 44-45 0.3489706479911021 1106 46-47 -0.09826019961921872 1106 48-49 0.21306943804408718 1106 50-51 0.34277733928259124 1106 52-53 0.2681818768882067 1106 54-55 0.17712608576545108 1106 56-57 0.2045126125177248 1106 58-59 0.3021445279821293 1106 60-61 0.35491828189862673 1106 62-63 0.08004949335777667 1106 64-65 -0.11776269650503934 1106 66-67 -0.10130446706001095 1106 68-69 -0.22484886376971502 1106 70-71 -0.17974925263298758 1106 72-73 -0.1406219770117545 1106 74-75 0.09333953204126288 1106 76-77 0.0663927173553347 1106 78-79 0.05946080562043221 1106 80-81 -9.240272258068671E-4 1106 82-83 -0.005779497104882125 1106 84-85 0.054843831859507475 1106 86-87 -0.05272373103041872 1106 88-89 0.25885250970264195 1106 90-91 0.17932644145070498 1106 92-93 0.07309421048102394 1106 94-95 0.2008587800556043 1106 96-97 0.1298877777499854 1106 98-99 0.3484590674210679 1106 100-101 0.2059041188448063 1107 1 0.22280896689696306 1107 2 0.20442591745946004 1107 3 0.241000070684251 1107 4 0.06781348114084551 1107 5 0.12794531427523026 1107 6 -0.08584984200278711 1107 7 0.029265454782716915 1107 8 0.06122042934764238 1107 9 0.16557073541419953 1107 10-11 0.10524611152585095 1107 12-13 0.18084296155711854 1107 14-15 0.1390691426946944 1107 16-17 0.12139479734734238 1107 18-19 0.1633331337516708 1107 20-21 0.1370512175221421 1107 22-23 0.06374279758230017 1107 24-25 0.022468268647010348 1107 26-27 0.14009054572347424 1107 28-29 0.09471869164017477 1107 30-31 -0.06650379493493119 1107 32-33 0.1521154091504897 1107 34-35 -0.10442207536998183 1107 36-37 -0.18021849974034154 1107 38-39 -0.3485539712116221 1107 40-41 -0.39861871952948746 1107 42-43 -0.36989652460485445 1107 44-45 -0.31803634624932897 1107 46-47 -0.31876872363774567 1107 48-49 -0.1766746039620557 1107 50-51 0.007060582276480432 1107 52-53 -0.17239421515625963 1107 54-55 -0.226111209614011 1107 56-57 -0.3650464993290683 1107 58-59 -0.2635239952223358 1107 60-61 -0.16470086780254434 1107 62-63 -0.28407839744794927 1107 64-65 0.03960771331267665 1107 66-67 -0.07978920659069644 1107 68-69 -0.12111227052786688 1107 70-71 -0.19258446398659146 1107 72-73 0.08940441250842213 1107 74-75 0.14203464150011058 1107 76-77 0.03135028382097005 1107 78-79 0.3322090468844152 1107 80-81 0.22870210993185935 1107 82-83 0.33920411968401254 1107 84-85 0.26245269711014174 1107 86-87 0.10366979166524715 1107 88-89 0.16398252388108148 1107 90-91 0.2603368412281881 1107 92-93 0.30464359416022546 1107 94-95 0.22922242628678902 1107 96-97 0.12710297105830648 1107 98-99 0.2385253464987258 1107 100-101 0.04019964076710281 1108 1 0.12851748183264533 1108 2 0.0881795677449162 1108 3 0.18562203555396906 1108 4 0.1304576947739946 1108 5 -0.07372553159049033 1108 6 0.0412957754660539 1108 7 -0.01634513557865347 1108 8 -0.07055878387905068 1108 9 0.011398935940718502 1108 10-11 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-0.3604282374900123 2316 64-65 -1.18082011847752 2316 66-67 -0.4986718179597389 2316 68-69 -1.180421466104299 2316 70-71 -0.6044204603151258 2316 72-73 -0.6109128299771172 2316 74-75 -0.4473724893267672 2316 76-77 -0.6135119028352349 2316 78-79 -0.6279485050016618 2316 80-81 -0.45800311298444996 2316 82-83 -0.418789321594943 2316 84-85 -0.4431043931703016 2316 86-87 -0.37477153184189405 2316 88-89 -0.3384658573802284 2316 90-91 -0.795102619816042 2316 92-93 -0.46790200944707294 2316 94-95 -0.4965321385295951 2316 96-97 -0.3250655952017141 2316 98-99 -0.4631219083735374 2316 100-101 -0.20334411819990095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 3.0 14 26.0 15 110.0 16 317.0 17 779.0 18 1306.0 19 1726.0 20 2458.0 21 3253.0 22 3804.0 23 3767.0 24 3250.0 25 2802.0 26 2596.0 27 2695.0 28 3070.0 29 3843.0 30 3822.0 31 4273.0 32 5304.0 33 6668.0 34 9699.0 35 16861.0 36 29118.0 37 74416.0 38 157146.0 39 61905.0 40 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.84670686513734 20.921993705700718 14.777017154808611 22.454282274353325 2 15.010676771541775 22.194847994865274 40.96374834911192 21.830726884481034 3 16.97396842637965 27.374995371341814 33.196242763864376 22.45479343841416 4 11.553377686164632 19.75437253909673 40.124171470185274 28.568078304553364 5 11.522026241406124 38.54376242023279 37.098139896564916 12.836071441796165 6 27.235024747892417 39.85459841761605 18.801239246084155 14.109137588407371 7 23.630596047743065 35.042275078070034 24.20454966241653 17.12257921177037 8 23.289679943715516 35.97417825888394 22.594270338324055 18.14187145907649 9 24.6940765518348 15.881111371687423 22.696717972771147 36.72809410370663 10-11 22.119308293321154 27.220213041707296 31.128034856881886 19.53244380808966 12-13 23.40842045496624 25.93505066837824 30.507794660379922 20.148734216275596 14-15 21.094214794425863 27.54927978078675 28.440697631361317 22.915807793426072 16-17 19.380623819692165 30.68479454929212 29.426786970635792 20.507794660379922 18-19 19.344088277768865 28.929483935470333 32.19336682424676 19.53306096251404 20-21 20.002838910352146 28.00720836367676 32.49095868768283 19.498994038288263 22-23 19.7473369786588 28.38453657874273 32.153498648431814 19.714627794166656 24-25 19.790044064825903 28.480195514521643 32.18114716664404 19.54861325400842 26-27 19.59280151079403 28.648802103262277 32.102151400323386 19.6562449856203 28-29 19.347050619005888 28.072009578236667 32.480837355123 20.100102447634445 30-31 19.649703148721873 28.17038399348285 32.48355283459027 19.696360023205006 32-33 19.329523433353494 28.461557450905367 32.35123492600319 19.857684189737956 34-35 19.577619511954282 28.60226865966402 32.328893935840625 19.491217892541073 36-37 19.800165397385733 28.49204487946974 31.734944517817247 19.97284520532728 38-39 19.659824481281706 28.345162126467283 32.05907402150166 19.93593937074935 40-41 19.78066331757532 28.257279336435566 32.09585642519471 19.8662009207944 42-43 19.839243020266192 28.422041381591693 31.77517721654918 19.96353838159293 44-45 19.771652862979376 28.800251799005146 31.47882543169952 19.94926990631596 46-47 20.09820197564206 28.486225679396966 30.983735452073464 20.431836892887517 48-49 20.039128314952077 28.576753831057022 30.804969419432084 20.57914843455882 50-51 20.087142742527757 28.70378256281128 30.68054038374899 20.528534310911976 52-53 19.733017348317937 28.96743256003407 30.873217184153845 20.426332907494153 54-55 19.85015490576052 28.94108643864868 30.58839502820396 20.620363627386844 56-57 20.19045385536369 28.950837478553883 30.143426688225926 20.7152819778565 58-59 19.81608798153474 29.01786044904156 30.630731821716427 20.53531974770727 60-61 20.106273991878247 29.19843983361517 30.07553970154412 20.619746472962465 62-63 19.690929064270463 29.43764888850488 30.284261327869462 20.587160719355197 64-65 19.53984966118222 29.39086858313687 30.172186084402043 20.89709567127887 66-67 19.665378871101126 29.430243035412325 29.928039794117282 20.976338299369267 68-69 19.708355345337488 29.22198468458238 29.926039666042925 21.14362030403721 70-71 19.731414394509795 28.994778873569743 29.878420578397126 21.395386153523337 72-73 19.680314008171123 28.826419146598862 29.810410160830443 21.68285668439957 74-75 19.691175926040213 28.939605268030167 29.62637471148031 21.742844094449314 76-77 19.545033758347014 28.794573978300853 29.83867583346705 21.821716429885086 78-79 19.523556784378588 28.65090042830517 29.652788920843776 22.17275386647247 80-81 19.511954281200243 28.787414986978042 29.516274362170904 22.184356369650814 82-83 19.595887282915932 28.505869138575857 29.4766530481257 22.421590530382513 84-85 19.383215868274558 28.39799054519422 29.50751076934471 22.711282817186518 86-87 19.283977436834245 28.537467445104113 29.530468913931646 22.648086204129996 88-89 19.45937272424306 28.117679005640788 29.581939592924943 22.84100867719121 90-91 19.40802547613464 28.03251169507634 29.474431292197934 23.085031536591085 92-93 19.279904217633337 28.056333855857414 29.46023674043719 23.203525186072056 94-95 19.028845797795523 27.787748250367205 29.814977103570854 23.36842884826641 96-97 18.980707752693878 27.807373761062493 29.694138267277236 23.51778021896639 98-99 18.98996506905958 27.693817346976562 29.524173938802967 23.79204364516089 100-101 18.868185364023265 27.621061310740487 29.571520968490482 23.939232356745762 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1321.0 1 792.5 2 316.5 3 387.5 4 296.5 5 219.0 6 327.0 7 584.0 8 944.0 9 1151.5 10 904.0 11 586.5 12 560.0 13 588.5 14 644.5 15 709.0 16 734.0 17 754.5 18 865.0 19 976.0 20 1103.5 21 1313.0 22 1546.0 23 1850.0 24 2236.5 25 2789.5 26 3482.5 27 4240.0 28 4944.0 29 5813.5 30 6852.5 31 7833.0 32 9049.5 33 10416.5 34 11347.5 35 12133.5 36 13333.0 37 14399.0 38 14995.5 39 15752.5 40 16246.5 41 16182.0 42 16078.0 43 16217.5 44 16448.0 45 16375.5 46 15815.0 47 15342.0 48 14800.0 49 13836.5 50 13054.0 51 11761.5 52 10227.0 53 9343.0 54 8330.5 55 7073.5 56 6007.0 57 4993.5 58 4163.0 59 3419.5 60 2675.0 61 2100.5 62 1695.5 63 1249.0 64 864.0 65 619.0 66 419.5 67 286.0 68 226.0 69 194.0 70 153.5 71 120.0 72 91.0 73 72.0 74 56.5 75 32.5 76 24.5 77 16.5 78 14.5 79 12.0 80 4.5 81 3.0 82 4.0 83 3.0 84 2.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14614216769320018 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 3.7029265462804105E-4 44-45 0.0 46-47 3.7029265462804105E-4 48-49 0.0018514632731402051 50-51 6.171544243800684E-4 52-53 6.171544243800684E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.405853092560821E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.4686176975202733E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 405085.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.28808177140632 #Duplication Level Percentage of deduplicated Percentage of total 1 71.38647232014102 42.323670082887546 2 16.35593992789854 19.394246077869166 3 5.888610071744205 10.473731863604915 4 2.6198077173968026 6.212934966975318 5 1.3501478255090316 4.002383734113295 6 0.7619490083619918 2.710469706804463 7 0.4604365466633888 1.9108879740386053 8 0.2861485766619608 1.357216016952469 9 0.18391934090868586 0.981380243082359 >10 0.6783785320484065 6.289094939802613 >50 0.017671426448597302 0.7373847404281213 >100 0.007573468476510874 0.9232276467966356 >500 0.0012622447460851457 0.6094150610981304 >1k 0.001682992994780194 2.0739569455464006 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2748 0.6783761432785712 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2542 0.6275226187096535 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1694 0.41818383795993436 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1330 0.32832615377019636 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 898 0.22168186923732058 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 877 0.21649777207252802 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 668 0.16490366219435426 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 444 0.10960662576990014 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 406 0.1002258785193231 RNA PCR Primer, Index 39 (95% over 23bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.4686176975202733E-4 0.0 10-11 0.0 0.0 0.0 2.4686176975202733E-4 0.0 12-13 0.0 0.0 0.0 2.4686176975202733E-4 0.0 14-15 0.0 0.0 0.0 3.70292654628041E-4 0.0 16-17 0.0 0.0 0.0 8.640161941320957E-4 0.0 18-19 0.0 0.0 0.0 9.874470790081093E-4 0.0 20-21 0.0 0.0 0.0 0.0013577397336361503 0.0 22-23 0.0 0.0 0.0 0.003826357431156424 0.0 24-25 0.0 0.0 0.0 0.008516731056444944 0.0 26-27 0.0 0.0 0.0 0.015058567954873668 0.0 28-29 0.0 0.0 0.0 0.03283261537701964 0.0 30-31 0.0 0.0 0.0 0.057889085006850416 0.0 32-33 0.0 0.0 0.0 0.09146228569312613 0.0 34-35 0.0 0.0 0.0 0.14749990742683633 0.0 36-37 0.0 0.0 0.0 0.22698939728698914 0.0 38-39 0.0 0.0 0.0 0.343137859955318 0.0 40-41 0.0 0.0 0.0 0.46953108606835603 0.0 42-43 0.0 0.0 0.0 0.577409679449992 0.0 44-45 0.0 0.0 0.0 0.6962736215855931 0.0 46-47 0.0 0.0 0.0 0.8504387967957343 0.0 48-49 0.0 0.0 0.0 1.0134909957169482 0.0 50-51 0.0 0.0 0.0 1.1681498944665933 0.0 52-53 0.0 0.0 0.0 1.326635150647395 0.0 54-55 0.0 0.0 0.0 1.506720811681499 0.0 56-57 0.0 0.0 0.0 1.7419800782551809 0.0 58-59 0.0 0.0 0.0 1.996000839330017 0.0 60-61 0.0 0.0 0.0 2.2531073725267534 0.0 62-63 0.0 0.0 0.0 2.4944147524593605 0.0 64-65 0.0 0.0 0.0 2.762012910870558 0.0 66-67 0.0 0.0 0.0 3.089475048446622 0.0 68-69 0.0 0.0 0.0 3.4617425972326794 0.0 70-71 0.0 0.0 0.0 3.8733845982941855 0.0 72-73 0.0 0.0 0.0 4.284286014046435 0.0 74-75 0.0 0.0 0.0 4.7039510226248815 0.0 76-77 0.0 0.0 0.0 5.163731068788032 0.0 78-79 0.0 0.0 0.0 5.689299776590099 0.0 80-81 0.0 0.0 0.0 6.258069294098769 0.0 82-83 0.0 0.0 0.0 6.853253020970907 0.0 84-85 0.0 0.0 0.0 7.4590518039423825 0.0 86-87 0.0 0.0 0.0 8.140143426688226 0.0 88-89 0.0 0.0 0.0 8.857030006048113 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTCC 20 0.002073446 71.24704 6 GGTATCA 1115 0.0 58.042282 1 GAACCGG 45 9.6196495E-4 42.220467 6 CGCAGAA 195 0.0 38.97274 9 GTATCAA 1720 0.0 37.83273 2 CTACGCT 60 0.003948761 31.665352 4 TCAACGC 2165 0.0 29.398317 5 ATCAACG 2210 0.0 29.014631 4 CAACGCA 2215 0.0 28.7347 6 AACGCAG 2255 0.0 28.435625 7 TATCAAC 2260 0.0 28.372715 3 ACGCAGA 2470 0.0 25.57586 8 CGCAGAG 2385 0.0 25.093294 8 CATGGGG 460 0.0 22.716448 4 GGACAGT 210 3.4337063E-8 22.618109 6 GCAGAGT 2665 0.0 22.100395 9 ACTACTA 130 2.9377834E-4 21.922165 5 CCTATTC 130 2.9377834E-4 21.922165 3 GTATACA 110 0.0028441173 21.630062 1 GTAGCAC 110 0.002874878 21.590012 3 >>END_MODULE