FastQCFastQC Report
Fri 10 Feb 2017
SRR2031816_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031816_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42170
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4781.1335072326298317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4601.0908228598529761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.5525254920559639No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.14939530471899454No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.10196822385582166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATC200.002060199971.254
GGTATCA4000.038.0000041
GTATCAA5800.035.2155151
AACGCAG6000.033.256
ATCAACG6300.032.420633
TCAACGC6300.032.420634
TATCAAC6500.031.4230792
CAACGCA6350.031.417325
ACGCAGA6850.029.1240867
CGCAGAG6850.028.4306568
ATGGGGC700.00830177627.1428597
GCAGAGT7000.025.1071419
GAGTACT3800.024.37512-13
CAGAGTA6700.023.04104610-11
GTACTTT4050.022.87036914-15
TTGGTTT550.002855543521.5909154-55
ACTTTTT4650.020.43010916-17
AGTACTT3750.020.26666612-13
GTACATG3750.019.01
TACATGG3757.2759576E-1219.02
Produced by FastQC (version 0.11.5)