##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031816_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42170 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.757007351197533 33.0 30.0 34.0 12.0 34.0 2 28.82900165994783 33.0 30.0 34.0 12.0 34.0 3 28.85859615840645 33.0 30.0 34.0 12.0 34.0 4 31.6724448660185 37.0 33.0 37.0 12.0 37.0 5 31.695921271045766 37.0 33.0 37.0 12.0 37.0 6 31.873345980554898 37.0 33.0 37.0 12.0 37.0 7 31.892459094142755 37.0 33.0 37.0 12.0 37.0 8 31.879274365662795 37.0 33.0 37.0 12.0 37.0 9 33.36656390799146 39.0 34.0 39.0 12.0 39.0 10-11 33.34119041972967 39.0 34.0 39.0 12.0 39.0 12-13 33.24357363054304 39.0 34.0 39.0 11.5 39.0 14-15 34.32893051932653 40.0 33.5 41.0 11.0 41.0 16-17 34.2296537823097 40.0 33.5 41.0 10.0 41.0 18-19 34.32641688404078 40.0 34.0 41.0 10.0 41.0 20-21 34.30476642162675 40.0 34.0 41.0 10.0 41.0 22-23 34.14628883092246 40.0 34.0 41.0 10.0 41.0 24-25 34.02990277448423 40.0 33.0 41.0 10.0 41.0 26-27 33.79090585724448 40.0 33.0 41.0 9.5 41.0 28-29 33.53102916765474 40.0 32.0 41.0 9.0 41.0 30-31 33.1404671567465 39.0 31.5 41.0 8.5 41.0 32-33 32.71907754327721 38.0 30.5 41.0 8.0 41.0 34-35 32.38144415461228 38.0 30.0 41.0 8.0 41.0 36-37 32.050818117144885 38.0 29.0 41.0 8.0 41.0 38-39 31.714275551339817 37.0 27.5 40.5 8.0 41.0 40-41 31.368757410481386 37.0 26.5 40.0 8.0 41.0 42-43 30.919314678681527 36.0 24.5 40.0 8.0 41.0 44-45 30.643988617500593 36.0 22.5 40.0 7.5 41.0 46-47 30.006473796537822 35.5 19.0 40.5 7.0 41.0 48-49 29.328598529760495 35.0 16.0 40.0 7.0 41.0 50-51 28.26158406450083 34.5 14.0 39.0 7.0 40.0 52-53 28.166054066872185 35.0 13.0 39.0 7.0 40.5 54-55 28.530009485416173 35.0 13.0 40.0 7.0 41.0 56-57 28.309746265117383 35.0 13.0 40.0 7.0 41.0 58-59 27.98440834716623 35.0 12.5 39.5 7.0 41.0 60-61 27.72212473322267 35.0 12.0 39.0 7.0 41.0 62-63 27.39809105999526 35.0 11.5 38.5 7.0 41.0 64-65 27.018437277685557 35.0 11.0 37.0 7.0 41.0 66-67 26.657232629831633 34.0 10.0 37.0 7.0 40.0 68-69 26.27172160303533 34.0 10.0 36.0 7.0 39.5 70-71 25.856782072563433 34.0 10.0 35.0 7.0 39.0 72-73 25.452976049324164 33.0 10.0 35.0 7.0 38.5 74-75 25.14213896134693 33.0 10.0 35.0 7.0 37.0 76-77 24.82054778278397 33.0 10.0 35.0 7.0 37.0 78-79 24.50149395304719 32.5 8.0 35.0 7.0 36.0 80-81 24.245861987194687 32.0 8.0 35.0 7.0 36.0 82-83 23.993217927436568 31.5 8.0 35.0 7.0 35.0 84-85 23.77863410007114 31.0 8.0 35.0 7.0 35.0 86-87 23.590040313018733 31.0 8.0 35.0 7.0 35.0 88-89 23.39918188285511 30.0 8.0 35.0 7.0 35.0 90-91 23.205762390324875 30.0 8.0 35.0 7.0 35.0 92-93 22.982677258714723 29.0 7.5 35.0 7.0 35.0 94-95 22.75147023950676 29.0 7.0 35.0 7.0 35.0 96-97 22.588842779226937 29.0 7.0 35.0 7.0 35.0 98-99 22.429025373488262 27.5 7.0 35.0 7.0 35.0 100-101 21.074994071614892 23.5 7.0 34.0 5.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5857227335038324 1101 2 -0.9958754080939762 1101 3 -1.0453216163001606 1101 4 -0.49438680820585645 1101 5 -0.7424117201484712 1101 6 -0.16954739376317818 1101 7 -0.6637467558542589 1101 8 0.6025724916282797 1101 9 0.5723119525266256 1101 10-11 0.6854089853695697 1101 12-13 0.18016170859392133 1101 14-15 0.10950393704694505 1101 16-17 -0.7509651083694635 1101 18-19 -0.7426216245789377 1101 20-21 -1.0101973151886483 1101 22-23 -1.9546199904572816 1101 24-25 -1.5582730349124105 1101 26-27 -1.6238321509552947 1101 28-29 -1.1996398849905248 1101 30-31 -2.26183349783107 1101 32-33 -2.2542344415609463 1101 34-35 -1.8830695294935182 1101 36-37 -1.053298552466412 1101 38-39 -0.5583395686841506 1101 40-41 -1.1203333778432416 1101 42-43 -0.8566628810307826 1101 44-45 -0.6392997631509694 1101 46-47 -0.13186636882198854 1101 48-49 0.7160384043906163 1101 50-51 1.2927584046859266 1101 52-53 1.067127296019251 1101 54-55 1.7616716828769334 1101 56-57 1.6656285565112263 1101 58-59 0.6187406759666949 1101 60-61 0.11054635543702318 1101 62-63 0.10551713040938182 1101 64-65 0.18141943578442365 1101 66-67 0.9423711613846315 1101 68-69 0.5394012070668985 1101 70-71 -0.5330394333508615 1101 72-73 0.19164237656937289 1101 74-75 0.55756894846402 1101 76-77 0.5283141741058905 1101 78-79 0.13962801309358852 1101 80-81 1.0602622992531003 1101 82-83 0.9141100984756143 1101 84-85 0.5793854572755066 1101 86-87 1.88682788376072 1101 88-89 1.576738269207624 1101 90-91 0.057749123445237416 1101 92-93 0.8775210840540808 1101 94-95 1.4949287858405675 1101 96-97 1.9984809037982778 1101 98-99 -0.2104513072716685 1101 100-101 0.04112040436459097 1102 1 1.1955272664961676 1102 2 0.6291245919060238 1102 3 1.9859283836998394 1102 4 2.4118631917941435 1102 5 1.0700882798515288 1102 6 0.6429526062368218 1102 7 0.6487532441457411 1102 8 0.9150724916282797 1102 9 1.6348119525266256 1102 10-11 1.7166589853695697 1102 12-13 1.8989117085939213 1102 14-15 1.953253937046945 1102 16-17 2.3271598916305365 1102 18-19 1.5698783754210623 1102 20-21 1.6616776848113517 1102 22-23 1.7016300095427184 1102 24-25 2.0823519650875895 1102 26-27 1.6886678490447053 1102 28-29 2.3628601150094752 1102 30-31 2.58191650216893 1102 32-33 2.4957655584390537 1102 34-35 2.1481804705064818 1102 36-37 2.243576447533588 1102 38-39 2.5197854313158494 1102 40-41 1.8171666221567584 1102 42-43 1.8777121189692174 1102 44-45 2.3607002368490306 1102 46-47 3.9306336311780115 1102 48-49 3.8410384043906163 1102 50-51 2.4177584046859266 1102 52-53 3.395252296019251 1102 54-55 3.9335466828769334 1102 56-57 3.7437535565112263 1102 58-59 3.431240675966695 1102 60-61 3.329296355437023 1102 62-63 3.918017130409382 1102 64-65 3.9782944357844237 1102 66-67 3.8642461613846315 1102 68-69 3.8362762070668985 1102 70-71 3.2482105666491385 1102 72-73 3.097892376569373 1102 74-75 3.44819394846402 1102 76-77 4.2939391741058905 1102 78-79 3.2177530130935885 1102 80-81 2.5915122992531003 1102 82-83 2.5859850984756143 1102 84-85 3.3450104572755066 1102 86-87 3.15245288376072 1102 88-89 2.514238269207624 1102 90-91 3.2608741234452374 1102 92-93 3.471271084054081 1102 94-95 3.8543037858405675 1102 96-97 3.685980903798278 1102 98-99 3.8676736927283315 1102 100-101 2.759870404364591 1103 1 2.8353298980751163 1103 2 2.895571960327075 1103 3 2.2984283836998394 1103 4 1.4677842444257259 1103 5 2.7806145956410013 1103 6 1.6199262904473493 1103 7 1.9842795599352137 1103 8 1.5302040705756497 1103 9 0.9802066893687282 1103 10-11 2.0390274064222 1103 12-13 1.567497234909716 1103 14-15 2.00670788441537 1103 16-17 2.0574230495252763 1103 18-19 3.0698783754210623 1103 20-21 3.270230316390297 1103 22-23 2.1185707990164033 1103 24-25 2.5108059124560143 1103 26-27 2.3885033753605 1103 28-29 1.624373272904215 1103 30-31 1.0235283442741903 1103 32-33 1.5738905584390537 1103 34-35 3.1958778389275366 1103 36-37 3.9138067106914782 1103 38-39 2.620114378684274 1103 40-41 3.6255547800514982 1103 42-43 4.526560803179745 1103 44-45 4.603298921059558 1103 46-47 5.2743836311780115 1103 48-49 5.194656825443246 1103 50-51 4.495061036264872 1103 52-53 5.031765453913991 1103 54-55 5.314303261824303 1103 56-57 5.286516714405966 1103 58-59 4.940286728598277 1103 60-61 5.173868723858078 1103 62-63 5.309464498830437 1103 64-65 4.646879962100211 1103 66-67 5.199772477174104 1103 68-69 4.594499891277426 1103 70-71 4.323868461385981 1103 72-73 4.970425271306215 1103 74-75 4.126647895832441 1103 76-77 4.798873384632206 1103 78-79 4.452950381514643 1103 80-81 4.7650320360952065 1103 82-83 4.216741677422984 1103 84-85 4.090898615170243 1103 86-87 4.150808146918614 1103 88-89 3.4089751113128877 1103 90-91 3.7312688602873436 1103 92-93 3.7648566103698684 1103 94-95 3.5195998384721463 1103 96-97 4.201605903798278 1103 98-99 3.8931671137809616 1103 100-101 3.6899690885751184 1104 1 0.9783798305987332 1104 2 1.3422656175470493 1104 3 1.9907360760075328 1104 4 2.0352606276915814 1104 5 1.4162421260053755 1104 6 1.6381449139291284 1104 7 1.4788814492739455 1104 8 2.2211622352180243 1104 9 1.1300042602189322 1104 10-11 1.3031974469080296 1104 12-13 1.5114918367990526 1104 14-15 2.362308424226434 1104 16-17 1.785493224963865 1104 18-19 1.64039119593388 1104 20-21 2.528664864298534 1104 22-23 2.7973832146709228 1104 24-25 2.0779449138055384 1104 26-27 2.1978825926344427 1104 28-29 2.2947511406504972 1104 30-31 1.603150476527908 1104 32-33 1.8431213276698273 1104 34-35 1.6874432910192994 1104 36-37 1.9050347808669166 1104 38-39 2.0910995338799516 1104 40-41 1.7574711093362474 1104 42-43 2.583240965123064 1104 44-45 1.9869021599259504 1104 46-47 1.6461785029728873 1104 48-49 1.0613909684931784 1104 50-51 1.7919571226346456 1104 52-53 2.138040757557711 1104 54-55 0.42833834954359773 1104 56-57 0.508176633434303 1104 58-59 1.360327214428235 1104 60-61 1.9282546887703589 1104 62-63 0.7625684124606664 1104 64-65 0.7062591793741682 1104 66-67 0.5982205203589892 1104 68-69 0.08066723270792409 1104 70-71 1.304300310238883 1104 72-73 0.8290622483642451 1104 74-75 1.2194279228229945 1104 76-77 0.5880096869264015 1104 78-79 -0.8183046792141049 1104 80-81 -0.7774780853622865 1104 82-83 -0.226915542550028 1104 84-85 -0.5143645427244934 1104 86-87 -0.17206634700851353 1104 88-89 -1.184479679510325 1104 90-91 -0.13175408168296698 1104 92-93 0.9973127507207451 1104 94-95 1.0101531448149252 1104 96-97 -0.01634281415043759 1104 98-99 -0.810611563681924 1104 100-101 -1.5930943392251535 1105 1 3.6087217109406104 1105 2 2.899957925239356 1105 3 3.4650950503665037 1105 4 4.210474302905258 1105 5 4.2089771687404145 1105 6 3.6082303840146004 1105 7 4.079308799701298 1105 8 3.5609058249616155 1105 9 3.794534174748847 1105 10-11 3.9805478742584555 1105 12-13 4.46662004192725 1105 14-15 4.639017825935831 1105 16-17 4.716048780519422 1105 18-19 4.417100597643284 1105 20-21 4.876955462589123 1105 22-23 3.7589216762093898 1105 24-25 4.268115853976475 1105 26-27 4.19040396015582 1105 28-29 4.352443448342811 1105 30-31 4.2954581688356015 1105 32-33 4.546112780661275 1105 34-35 5.339152692728703 1105 36-37 4.210590336422474 1105 38-39 4.196868764649185 1105 40-41 3.5758471777123155 1105 42-43 4.30653156341366 1105 44-45 3.911047459071252 1105 46-47 4.538272520066897 1105 48-49 3.9000661821683877 1105 50-51 3.1990084046859266 1105 52-53 3.857057851574808 1105 54-55 4.761671682876933 1105 56-57 4.2368091120667835 1105 58-59 4.738532342633366 1105 60-61 4.344921355437023 1105 62-63 4.75829490818716 1105 64-65 4.7543361024510915 1105 66-67 4.975357272495742 1105 68-69 5.068915095955788 1105 70-71 4.90793278887136 1105 72-73 5.332267376569373 1105 74-75 4.411735615130688 1105 76-77 4.96407806299478 1105 78-79 4.582336346426921 1105 80-81 5.0654706325864325 1105 82-83 4.789110098475614 1105 84-85 6.221746568386617 1105 86-87 4.614258439316277 1105 88-89 3.569793824763181 1105 90-91 3.184485234556348 1105 92-93 4.052868306276302 1105 94-95 4.567845452507232 1105 96-97 4.646050348242721 1105 98-99 4.362465359394999 1105 100-101 4.872717626586812 1106 1 2.097204095764461 1106 2 2.1702831284913913 1106 3 2.3715991154071574 1106 4 1.4042412405746312 1106 5 1.9115516944856772 1106 6 2.062159923309995 1106 7 1.566436170975006 1106 8 1.9897676135795024 1106 9 1.2125558549656503 1106 10-11 1.744098009759817 1106 12-13 2.5540184159109955 1106 14-15 2.314153327290846 1106 16-17 1.7814281843134623 1106 18-19 2.210122277860087 1106 20-21 2.1540557335918464 1106 22-23 1.958108668079305 1106 24-25 1.6536172089900276 1106 26-27 2.2591708978252 1106 28-29 1.744719871107037 1106 30-31 0.3726939411933188 1106 32-33 1.2812076316097816 1106 34-35 1.3547353485552591 1106 36-37 1.3399941304604148 1106 38-39 0.8665842118036515 1106 40-41 1.6483404026445605 1106 42-43 2.139526143359465 1106 44-45 2.173581334410006 1106 46-47 1.6890177775194744 1106 48-49 0.8265566970735421 1106 50-51 0.7935205998078771 1106 52-53 1.1886974179704701 1106 54-55 1.2738668048281525 1106 56-57 1.1019852638283005 1106 58-59 0.8096705540154758 1106 60-61 0.5827262334858041 1106 62-63 1.3341756669947458 1106 64-65 0.657791387003936 1106 66-67 0.519733966262681 1106 68-69 1.7989286460912872 1106 70-71 1.6094910544540149 1106 72-73 1.54263323022791 1106 74-75 1.9279957777323098 1106 76-77 2.0142135643497916 1106 78-79 2.569124964313101 1106 80-81 1.3876251041311498 1106 82-83 0.8409393667682963 1106 84-85 0.6319769206901427 1106 86-87 -0.24617516501976766 1106 88-89 0.3252138789637229 1106 90-91 0.866057050274506 1106 92-93 1.7818655962492045 1106 94-95 0.8341056151088608 1106 96-97 1.1650205379446206 1106 98-99 1.5921401561429676 1106 100-101 1.4187880872914214 1107 1 0.43866751039860574 1107 2 0.7312587382474867 1107 3 0.8350137495534966 1107 4 1.2091192893551188 1107 5 1.1066736457051896 1107 6 1.1841111428221858 1107 7 1.273753244145741 1107 8 0.5507432233355942 1107 9 0.8223119525266256 1107 10-11 1.4270248390280997 1107 12-13 0.5540184159109955 1107 14-15 0.37512893704694505 1107 16-17 0.6106964769963881 1107 18-19 0.417439351030815 1107 20-21 0.33698256286012906 1107 22-23 0.4337184241768668 1107 24-25 0.6536172089900347 1107 26-27 1.1860001661178785 1107 28-29 0.8300857247655742 1107 30-31 1.2019622338762446 1107 32-33 1.769012509658566 1107 34-35 0.40351583636014254 1107 36-37 0.42535998411895903 1107 38-39 1.2446329922914607 1107 40-41 1.0263891831323697 1107 42-43 0.5541602897009277 1107 44-45 1.758947188068543 1107 46-47 0.1280421677633754 1107 48-49 -0.8441750102435321 1107 50-51 -0.8406257416555363 1107 52-53 -0.7259367283709928 1107 54-55 0.029964365803763826 1107 56-57 -1.2272830288546288 1107 58-59 -0.8244757874479411 1107 60-61 -1.2709323030995634 1107 62-63 -1.0316779915418373 1107 64-65 -0.28123300323996503 1107 66-67 0.08070957601877993 1107 68-69 0.018440841213237746 1107 70-71 0.3046130056735272 1107 72-73 1.469462498520592 1107 74-75 0.40360553382987163 1107 76-77 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2313 16-17 -0.5855759574260659 2313 18-19 -0.24615936042798836 2313 20-21 -1.4432751453773278 2313 22-23 -2.3700091414006756 2313 24-25 -2.2858673745350515 2313 26-27 -1.8853297924647308 2313 28-29 -1.0233427151792043 2313 30-31 -1.4466801959442783 2313 32-33 -0.10535472457981854 2313 34-35 -0.529295944587858 2313 36-37 -0.20188345812679032 2313 38-39 -1.634106078118112 2313 40-41 -2.3900857363338055 2313 42-43 -1.62140344706852 2313 44-45 -3.3701370273019116 2313 46-47 -4.235050331086139 2313 48-49 -4.021579520137685 2313 50-51 -3.7278783877669035 2313 52-53 -6.861233553037351 2313 54-55 -6.474177373726839 2313 56-57 -3.776293613300094 2313 58-59 -3.046058852335193 2313 60-61 -1.9145126068271274 2313 62-63 -1.8685394733642013 2313 64-65 -1.3297833944042559 2313 66-67 -2.0909425178606504 2313 68-69 -3.5204454910463063 2313 70-71 -2.9204215088225602 2313 72-73 -3.8917892272042103 2313 74-75 -3.2077612402152234 2313 76-77 -2.0757542221205263 2313 78-79 -2.2392045340762223 2313 80-81 -2.185315530935579 2313 82-83 -1.7368332977508025 2313 84-85 -2.3351192597056247 2313 86-87 -3.696014097371357 2313 88-89 -2.8301013534338857 2313 90-91 -2.870022102969859 2313 92-93 -2.735391651794977 2313 94-95 -2.292217440574529 2313 96-97 -2.477639379220591 2313 98-99 -2.6862767789697806 2313 100-101 -2.8117687465788066 2314 1 -1.032531244142131 2314 2 -0.2791200889450387 2314 3 -0.4036992758746294 2314 4 -1.634679361397346 2314 5 -1.8554436350420893 2314 6 -0.797206968231265 2314 7 -0.9602893090457485 2314 8 -2.096895593478102 2314 9 -2.0713050687499717 2314 10-11 -1.4881282486729823 2314 12-13 -0.8933090360869329 2314 14-15 -1.2312540416764577 2314 16-17 -1.1329464913481857 2314 18-19 -1.041823752238507 2314 20-21 -2.363588272635454 2314 22-23 -2.7800854159891983 2314 24-25 -1.8282198434230494 2314 26-27 -1.4925156615935933 2314 28-29 -2.1776983956288234 2314 30-31 -2.5520595616608546 2314 32-33 -2.702705186241797 2314 34-35 -0.5266865507701155 2314 36-37 -0.8325538716153495 2314 38-39 -1.873166696343727 2314 40-41 -1.3892828459283493 2314 42-43 -0.8799341576265256 2314 44-45 -0.3404300822999069 2314 46-47 -0.9702972198858184 2314 48-49 -1.5027115956093837 2314 50-51 -1.0749277655268372 2314 52-53 -1.206476427385006 2314 54-55 -1.0893921469103027 2314 56-57 -1.1538528264674959 2314 58-59 -0.42281517509713495 2314 60-61 -0.03273821903106011 2314 62-63 -0.04075946533529873 2314 64-65 0.8137332655716563 2314 66-67 0.8562674379803745 2314 68-69 0.9805581219605166 2314 70-71 0.847944609202333 2314 72-73 0.6700333340161784 2314 74-75 0.12804767186827704 2314 76-77 0.19221045070163356 2314 78-79 0.40591790671060934 2314 80-81 -0.8183946156405177 2314 82-83 -0.7029111781201287 2314 84-85 0.3047843934457184 2314 86-87 0.7289156497181679 2314 88-89 0.30679146069698504 2314 90-91 0.4563528468494944 2314 92-93 0.9370290627774835 2314 94-95 1.459689424138439 2314 96-97 0.05266973358551397 2314 98-99 -0.23505237110145671 2314 100-101 -0.42397268074179095 2315 1 2.5319243253196966 2315 2 2.3754481213177883 2315 3 2.9454872072292524 2315 4 2.756225936892182 2315 5 2.824990240635845 2315 6 1.8598643709427058 2315 7 2.489439518655548 2315 8 1.7324744524125961 2315 9 1.7634884231148575 2315 10-11 2.400482514781338 2315 12-13 1.010737688986076 2315 14-15 2.522187760576358 2315 16-17 2.7462775386893625 2315 18-19 2.123799944048507 2315 20-21 2.8491776848113517 2315 22-23 2.7589216762093898 2315 24-25 2.197854416067983 2315 26-27 2.9150771627701957 2315 28-29 3.4357767816761466 2315 30-31 3.0503601296199108 2315 32-33 2.9660474211841503 2315 34-35 1.7590873332515784 2315 36-37 2.4344465455728006 2315 38-39 2.027751117590359 2315 40-41 3.1526445633332294 2315 42-43 2.2272831974005918 2315 44-45 2.4853938643000113 2315 46-47 2.8037953958838955 2315 48-49 3.8502295808612104 2315 50-51 3.6328319340976947 2315 52-53 2.970619943078077 2315 54-55 3.2322599181710494 2315 56-57 2.4802731643543616 2315 58-59 2.7826499896921852 2315 60-61 2.751784100535062 2315 62-63 2.706007326487814 2315 64-65 3.1097282593138367 2315 66-67 2.6019912594238477 2315 68-69 2.604045814910034 2315 70-71 3.2077693901785516 2315 72-73 3.045502670687018 2315 74-75 3.4313434582679427 2315 76-77 2.496757801556871 2315 78-79 3.040669679760253 2315 80-81 1.8326274953315327 2315 82-83 2.237639510240321 2315 84-85 2.211125653353939 2315 86-87 2.5194871974862103 2315 88-89 2.73482650450174 2315 90-91 3.5733741234452374 2315 92-93 2.820842162485455 2315 94-95 3.347257217213116 2315 96-97 4.819252962621807 2315 98-99 3.2399163397871575 2315 100-101 2.2583385416194943 2316 1 0.4470641517420688 2316 2 -0.6014081949792214 2316 3 -0.35730932121819237 2316 4 0.17313368359742398 2316 5 1.4553341814908727 2316 6 -1.0302031314680953 2316 7 0.33932701463754356 2316 8 0.051342983431560185 2316 9 0.4288693295758037 2316 10-11 -0.9370295392205961 2316 12-13 0.14558179056113119 2316 14-15 -0.7232317186907622 2316 16-17 -0.039643387057985535 2316 18-19 -0.007990477037957078 2316 20-21 -0.20000264305750193 2316 22-23 0.5020910751164891 2316 24-25 1.448899096235131 2316 26-27 1.126423996585686 2316 28-29 -0.09974234400691984 2316 30-31 -0.0679298093064773 2316 32-33 0.47552990270134643 2316 34-35 0.6620124377195964 2316 36-37 0.09629161146801479 2316 38-39 -0.08523506048743101 2316 40-41 0.573570310681351 2316 42-43 0.4010215451987271 2316 44-45 0.3015301548818172 2316 46-47 0.700613139374731 2316 48-49 0.02187856832504309 2316 50-51 0.0658116833744522 2316 52-53 0.561748197658595 2316 54-55 -0.3366889728607738 2316 56-57 0.41486011388827393 2316 58-59 0.3792427251470194 2316 60-61 -0.17557044784166465 2316 62-63 0.3739597533602037 2316 64-65 -0.27503548224836294 2316 66-67 0.9195740302370901 2316 68-69 0.932075387394768 2316 70-71 -0.13601074482626885 2316 72-73 1.3916936060775704 2316 74-75 0.2040853419066444 2316 76-77 -0.105138694746568 2316 78-79 0.3740030130935885 2316 80-81 0.5661536926957247 2316 82-83 0.09443796732807286 2316 84-85 0.30530758842304806 2316 86-87 0.23083403130170055 2316 88-89 0.11669728560106662 2316 90-91 0.9258331398386801 2316 92-93 1.1503182152016223 2316 94-95 0.5118345235454846 2316 96-97 1.488491149699918 2316 98-99 1.257274102564395 2316 100-101 0.1668888469875398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 34.0 7 712.0 8 1299.0 9 862.0 10 719.0 11 771.0 12 779.0 13 737.0 14 615.0 15 492.0 16 500.0 17 646.0 18 662.0 19 860.0 20 1108.0 21 1300.0 22 1113.0 23 993.0 24 813.0 25 635.0 26 596.0 27 533.0 28 570.0 29 546.0 30 588.0 31 695.0 32 811.0 33 1067.0 34 1558.0 35 2432.0 36 4571.0 37 6655.0 38 5042.0 39 856.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.257054778278395 18.415935499170025 12.231444154612284 21.095565567939293 2 19.203225041498694 22.089162912022765 35.13161014939531 23.576001897083234 3 20.36993123073275 27.76618449134456 28.68389850604695 23.179985771875742 4 15.072326298316337 19.46407398624615 32.28124258951862 33.1823571259189 5 14.657339340763576 36.36708560588096 30.55963955418544 18.415935499170025 6 29.445103153900874 35.09841119279108 15.835902300213423 19.620583353094617 7 26.236661133507234 35.8714726108608 18.453877163860565 19.437989091771403 8 28.121887597818358 31.292387953521462 18.107659473559405 22.478064975100782 9 29.33127815982926 15.558453877163862 17.263457434194926 37.84681052881195 10-11 26.537823096988376 26.527152003794168 23.05904671567465 23.875978183542802 12-13 29.27555133981504 26.157220773061418 23.47521935024899 21.092008536874555 14-15 26.656390799146312 25.575053355465972 24.87906094379891 22.889494901588808 16-17 22.355940241878113 28.593787052406928 25.235949727294283 23.81432297842068 18-19 22.23737253972018 25.934313493004506 29.967986720417354 21.860327246857956 20-21 24.8624614654968 24.759307564619398 29.63718283139673 20.741048138487077 22-23 24.585013042447237 25.54541142992649 28.95541854398862 20.914156983637657 24-25 23.05667536163149 26.343372065449373 29.17595447000237 21.423998102916766 26-27 22.898980317761442 26.25800331989566 29.172397438937637 21.670618923405264 28-29 23.16338629357363 25.947355940241877 29.784206782072566 21.105050984111926 30-31 23.348351908940003 26.08252312070192 29.4783021105051 21.090822859852977 32-33 22.474507944036045 26.739388190656864 29.826891154849417 20.959212710457674 34-35 22.438937633388665 27.167417595447002 29.051458382736545 21.34218638842779 36-37 22.387953521460755 27.32985534740337 28.68745553711169 21.59473559402419 38-39 22.596632677258714 26.633862935736307 28.926962295470716 21.842542091534266 40-41 22.173345980554895 26.99075171923168 28.873606829499643 21.962295470713777 42-43 22.32155560825231 26.92553948304482 28.87242115247807 21.880483756224805 44-45 22.127104576713304 27.039364477116436 28.68389850604695 22.14963244012331 46-47 21.855584538771637 27.25160066397913 27.93929333649514 22.95352146075409 48-49 22.253972018022292 27.488736068294994 27.338155086554423 22.919136827128288 50-51 22.23974389376334 27.593075646193977 26.901825942613232 23.26535451742945 52-53 21.8923405264406 27.621531894711882 26.25207493478776 24.23405264405976 54-55 22.492293099359735 27.054778278396963 26.042210101968223 24.410718520275076 56-57 22.37728242826654 26.740573867678446 26.343372065449373 24.538771638605645 58-59 22.74009959686981 26.129950201565094 26.48683898506047 24.643111216504625 60-61 22.7638131373014 25.72444866018497 26.314915816931467 25.19682238558217 62-63 22.628645956841357 25.758833293810767 26.38487076120465 25.22764998814323 64-65 22.93810765947356 25.377045292862228 26.77021579321793 24.91463125444629 66-67 22.553948304481857 25.624851790372304 26.42044107185203 25.400758833293814 68-69 23.062603746739388 26.18804837562248 25.781361157220772 24.96798672041736 70-71 23.45624851790372 25.910599952572923 25.589281479724924 25.043870049798432 72-73 23.062603746739388 25.757647616789185 25.75527626274603 25.424472373725397 74-75 23.331752430637895 25.516955181408584 25.697178088688645 25.45411429926488 76-77 23.96727531420441 25.179037230258476 25.636708560588094 25.216978894949015 78-79 23.83092245672279 25.547782783969648 25.40787289542329 25.213421863884278 80-81 24.532843253497745 25.520512212473324 24.80436329143941 25.14228124258952 82-83 24.747450794403605 24.940716148921034 24.75693621057624 25.554896846099123 84-85 24.815034384633623 25.116196348114773 24.42138961346929 25.64737965378231 86-87 25.062840882143707 24.858904434432063 24.448660184965615 25.62959449845862 88-89 25.585724448660187 24.535214607540905 24.009959686981265 25.869101256817643 90-91 26.236661133507234 23.65425658050747 23.840407872895423 26.268674413089876 92-93 27.332226701446526 23.385107896608964 23.770452928622245 25.512212473322265 94-95 27.174531657576477 23.21792743656628 23.96964666824757 25.637894237609675 96-97 26.84017073749111 23.51197533791795 23.774009959686982 25.87384396490396 98-99 27.108133744368036 23.34953758596158 23.76215318947119 25.78017548019919 100-101 28.61868626986009 23.04126156035096 23.144415461228363 25.19563670856059 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 11.0 2 15.0 3 20.5 4 13.5 5 13.5 6 17.0 7 36.0 8 70.0 9 93.0 10 82.5 11 69.5 12 71.0 13 77.0 14 96.0 15 106.5 16 112.5 17 112.0 18 116.5 19 132.0 20 143.5 21 155.0 22 177.5 23 204.5 24 234.5 25 269.0 26 312.0 27 381.5 28 412.0 29 467.0 30 569.0 31 608.0 32 663.5 33 690.0 34 707.5 35 725.5 36 731.0 37 748.0 38 735.0 39 746.0 40 728.0 41 749.5 42 802.5 43 840.5 44 854.0 45 857.5 46 900.0 47 1000.5 48 1100.0 49 1196.0 50 1323.0 51 1465.0 52 1592.5 53 1525.5 54 1335.5 55 1183.5 56 1130.0 57 1090.5 58 1124.5 59 1139.0 60 1126.5 61 1174.0 62 1094.0 63 947.0 64 804.0 65 669.0 66 573.0 67 500.0 68 430.5 69 382.5 70 308.5 71 245.5 72 197.5 73 155.0 74 134.5 75 110.5 76 89.0 77 67.5 78 55.0 79 48.0 80 38.5 81 29.5 82 24.5 83 20.5 84 12.5 85 6.5 86 8.5 87 9.5 88 6.5 89 4.5 90 3.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 42170.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.5186151292388 #Duplication Level Percentage of deduplicated Percentage of total 1 96.29403973509933 86.20109082285985 2 2.5933774834437084 4.643111216504624 3 0.4794701986754967 1.2876452454351435 4 0.18013245033112582 0.6450082997391511 5 0.10596026490066225 0.47427080863172877 6 0.07417218543046358 0.39838747925065215 7 0.04768211920529801 0.2987906094379891 8 0.03443708609271523 0.24662082048849893 9 0.026490066225165563 0.21342186388427792 >10 0.15364238410596026 2.6654019445103154 >50 0.0026490066225165563 0.14939530471899454 >100 0.007947019867549669 2.7768555845387715 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 1.1335072326298317 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 1.0908228598529761 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.5525254920559639 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.14939530471899454 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.10196822385582166 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0047427080863172875 2 0.0 0.0 0.0 0.0 0.0047427080863172875 3 0.0 0.0 0.0 0.0 0.0047427080863172875 4 0.0 0.0 0.0 0.0 0.0047427080863172875 5 0.0 0.0 0.0 0.0 0.0047427080863172875 6 0.0 0.0 0.0 0.0 0.0047427080863172875 7 0.0 0.0 0.0 0.0 0.0047427080863172875 8 0.0 0.0 0.0 0.0 0.0047427080863172875 9 0.0 0.0 0.0 0.0 0.0047427080863172875 10-11 0.0 0.0 0.0 0.0 0.0047427080863172875 12-13 0.0 0.0 0.0 0.0 0.0047427080863172875 14-15 0.0 0.0 0.0 0.0 0.0047427080863172875 16-17 0.0 0.0 0.0 0.0 0.0047427080863172875 18-19 0.0 0.0 0.0 0.0 0.0047427080863172875 20-21 0.0 0.0 0.0 0.0023713540431586438 0.0047427080863172875 22-23 0.0 0.0 0.0 0.016599478302110504 0.0047427080863172875 24-25 0.0 0.0 0.0 0.1138249940716149 0.0047427080863172875 26-27 0.0 0.0 0.0 0.22290728005691252 0.0047427080863172875 28-29 0.0 0.0 0.0 0.4185439886175006 0.0047427080863172875 30-31 0.0 0.0 0.0 0.7564619397676073 0.0047427080863172875 32-33 0.0 0.0 0.0 1.0896371828313967 0.0047427080863172875 34-35 0.0 0.0 0.0 1.5674650225278635 0.0047427080863172875 36-37 0.0 0.0 0.0 2.243300924828077 0.0047427080863172875 38-39 0.0 0.0 0.0 2.9784206782072564 0.0047427080863172875 40-41 0.0 0.0 0.0 4.07280056912497 0.0047427080863172875 42-43 0.0 0.0 0.0 4.6680104339577895 0.0047427080863172875 44-45 0.0 0.0 0.0 5.067583590230021 0.0047427080863172875 46-47 0.0 0.0 0.0 5.503912734171212 0.0047427080863172875 48-49 0.0 0.0 0.0 5.975812188759782 0.0047427080863172875 50-51 0.0 0.0 0.0 6.38012805311833 0.0047427080863172875 52-53 0.0 0.0 0.0 6.638605643822623 0.0047427080863172875 54-55 0.0 0.0 0.0 6.959924116670619 0.0047427080863172875 56-57 0.0 0.0 0.0 7.352383210813374 0.0047427080863172875 58-59 0.0 0.0 0.0 7.654730851316101 0.0047427080863172875 60-61 0.0 0.0 0.0 7.9049087028693386 0.0047427080863172875 62-63 0.0 0.0 0.0 8.133744368034147 0.0047427080863172875 64-65 0.0 0.0 0.0 8.389850604695281 0.0047427080863172875 66-67 0.0 0.0 0.0 8.663741996680105 0.0047427080863172875 68-69 0.0 0.0 0.0 8.976760730377045 0.0047427080863172875 70-71 0.0 0.0 0.0 9.205596395541855 0.0047427080863172875 72-73 0.0 0.0 0.0 9.414275551339815 0.0047427080863172875 74-75 0.0 0.0 0.0 9.626511738202513 0.0047427080863172875 76-77 0.0 0.0 0.0 9.866018496561537 0.0047427080863172875 78-79 0.0 0.0 0.0 10.08774009959687 0.0047427080863172875 80-81 0.0 0.0 0.0 10.339103628171685 0.0047427080863172875 82-83 0.0 0.0 0.0 10.588095802703343 0.0047427080863172875 84-85 0.0 0.0 0.0 10.82404552999763 0.0047427080863172875 86-87 0.0 0.0 0.0 11.15366374199668 0.0047427080863172875 88-89 0.0 0.0 0.0 11.539008774009961 0.0047427080863172875 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGATC 20 0.0020601999 71.25 4 GGTATCA 400 0.0 38.000004 1 GTATCAA 580 0.0 35.215515 1 AACGCAG 600 0.0 33.25 6 ATCAACG 630 0.0 32.42063 3 TCAACGC 630 0.0 32.42063 4 TATCAAC 650 0.0 31.423079 2 CAACGCA 635 0.0 31.41732 5 ACGCAGA 685 0.0 29.124086 7 CGCAGAG 685 0.0 28.430656 8 ATGGGGC 70 0.008301776 27.142859 7 GCAGAGT 700 0.0 25.107141 9 GAGTACT 380 0.0 24.375 12-13 CAGAGTA 670 0.0 23.041046 10-11 GTACTTT 405 0.0 22.870369 14-15 TTGGTTT 55 0.0028555435 21.59091 54-55 ACTTTTT 465 0.0 20.430109 16-17 AGTACTT 375 0.0 20.266666 12-13 GTACATG 375 0.0 19.0 1 TACATGG 375 7.2759576E-12 19.0 2 >>END_MODULE