FastQCFastQC Report
Fri 10 Feb 2017
SRR2031816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42170
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT28596.779701209390562No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG18594.408347166231918RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT9662.29072800569125RNA PCR Primer, Index 39 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT9612.2788712354754566RNA PCR Primer, Index 39 (95% over 23bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA6831.6196348114773538RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6231.477353568887835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6031.429926488024662No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT4621.0955655679392935RNA PCR Primer, Index 39 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.758833293810766No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG2110.5003557031064738Illumina PCR Primer Index 11 (96% over 25bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC1470.3485890443443206Illumina PCR Primer Index 11 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.21342186388427792No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC810.19207967749585012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.15413801280531184No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC620.1470239506758359No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT520.12331041024424946RNA PCR Primer, Index 39 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.12331041024424946No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT450.10671093194213896Illumina PCR Primer Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGAA250.00498811756.9992877
GGTATCA4200.049.820381
CTTATAC8150.036.1774331
TTATACA8200.035.9141852
ACATGGA401.0659118E-535.62455716-17
GTATCAA5950.035.1256142
TATACAC8550.034.4440163
CATGGGG851.5955042E-533.528994
TACACAT9050.032.541035
ATACACA9100.032.3622324
TATCAAC6750.030.9625783
ATCAACG6850.030.510574
TCAACGC6850.030.510575
CAACGCA7000.029.8567716
AACGCAG6700.029.7757477
CACATCT10100.029.1580527
ACACATC10250.028.7313486
CGCAGAG7500.026.5996699
CATCTCC11100.026.5311999
ACATGGG2155.2750693E-1126.5112973
Produced by FastQC (version 0.11.5)