##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031816_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42170 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.634313493004505 34.0 31.0 34.0 31.0 34.0 2 32.78774009959687 34.0 31.0 34.0 31.0 34.0 3 32.767725871472614 34.0 31.0 34.0 31.0 34.0 4 36.25738676784444 37.0 37.0 37.0 35.0 37.0 5 36.239317050035574 37.0 37.0 37.0 35.0 37.0 6 36.32200616552051 37.0 37.0 37.0 35.0 37.0 7 36.25888072089163 37.0 37.0 37.0 35.0 37.0 8 36.31059995257292 37.0 37.0 37.0 35.0 37.0 9 38.085439886175 39.0 39.0 39.0 37.0 39.0 10-11 38.087360682949964 39.0 39.0 39.0 37.0 39.0 12-13 38.0628408821437 39.0 39.0 39.0 35.0 39.0 14-15 39.4221484467631 41.0 39.0 41.0 36.5 41.0 16-17 39.34447474507944 41.0 39.0 41.0 36.0 41.0 18-19 39.51158406450083 41.0 39.5 41.0 36.5 41.0 20-21 39.5807801754802 41.0 40.0 41.0 37.0 41.0 22-23 39.50094854161726 41.0 40.0 41.0 36.0 41.0 24-25 39.3287526677733 41.0 39.0 41.0 35.0 41.0 26-27 39.0207967749585 41.0 38.5 41.0 35.0 41.0 28-29 38.759212710457675 40.5 38.0 41.0 35.0 41.0 30-31 38.4909177140147 40.0 37.5 41.0 35.0 41.0 32-33 37.593206070666355 39.5 35.5 41.0 33.0 41.0 34-35 37.467773298553475 40.0 35.0 41.0 33.0 41.0 36-37 37.346265117382025 40.0 35.0 41.0 33.0 41.0 38-39 36.773535688878354 39.0 35.0 41.0 31.5 41.0 40-41 36.489791320844205 39.0 35.0 41.0 31.0 41.0 42-43 36.23137301399099 39.0 35.0 41.0 30.5 41.0 44-45 35.61667061892341 39.0 35.0 41.0 25.5 41.0 46-47 34.88131373013991 39.0 35.0 41.0 21.5 41.0 48-49 34.404375148209624 39.0 35.0 41.0 13.5 41.0 50-51 33.899691723974385 39.0 34.0 41.0 10.0 41.0 52-53 33.93024662082048 39.0 34.5 41.0 10.0 41.0 54-55 33.567820725634334 38.5 34.0 40.5 10.0 41.0 56-57 32.754458145601134 37.0 32.5 40.0 9.0 41.0 58-59 31.955845387716387 36.0 30.5 39.5 8.0 41.0 60-61 30.814856533080388 35.0 27.0 39.0 7.0 41.0 62-63 29.767085605880958 35.0 22.5 39.0 7.0 41.0 64-65 29.099502015650934 35.0 20.5 37.5 7.0 41.0 66-67 28.166943324638368 35.0 17.5 37.0 7.0 40.0 68-69 27.137313255869103 35.0 13.5 36.0 7.0 39.0 70-71 26.2253023476405 34.0 10.0 35.5 7.0 39.0 72-73 25.523298553474035 34.0 10.0 35.0 7.0 38.5 74-75 25.314382262271756 34.0 10.0 35.0 7.0 37.0 76-77 24.47015650936685 32.0 10.0 34.5 7.0 36.0 78-79 25.126416884040786 33.0 10.0 35.0 7.0 36.0 80-81 25.088285511026797 33.0 10.0 35.0 7.0 36.0 82-83 24.925895186151294 33.0 10.0 35.0 7.0 35.5 84-85 24.78749110742234 33.0 10.0 35.0 7.0 35.0 86-87 24.670002371354045 33.0 10.0 35.0 7.0 35.0 88-89 24.51217690301162 33.0 10.0 35.0 7.0 35.0 90-91 24.38872421152478 33.0 10.0 35.0 7.0 35.0 92-93 24.276962295470714 33.0 10.0 35.0 7.0 35.0 94-95 24.21897083234527 33.0 10.0 35.0 7.0 35.0 96-97 24.140479013516718 33.0 9.0 35.0 7.0 35.0 98-99 24.053758596158406 33.0 8.0 35.0 7.0 35.0 100-101 23.04957315627223 29.5 7.5 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10506191196042636 1101 2 0.08722106719582001 1101 3 0.28509361039440506 1101 4 0.0034310097789287397 1101 5 -0.503445532992437 1101 6 -0.8903074727822258 1101 7 -0.4316480450654936 1101 8 0.048560581260993274 1101 9 0.457881121696623 1101 10-11 0.1245620593326251 1101 12-13 0.26175110490284936 1101 14-15 0.2636951616613814 1101 16-17 0.11609488733618889 1101 18-19 0.19391339343038538 1101 20-21 0.12233175114801043 1101 22-23 0.10668188842342374 1101 24-25 -0.036830837300236396 1101 26-27 0.8634268843343591 1101 28-29 0.5466275680742143 1101 30-31 0.20672237764916446 1101 32-33 0.45573360327518486 1101 34-35 0.48144140584889783 1101 36-37 -0.30464053441301076 1101 38-39 -0.8860336081722409 1101 40-41 -1.4268853327356865 1101 42-43 -1.393694010770055 1101 44-45 -1.0417283897163259 1101 46-47 -0.9444721163894556 1101 48-49 -0.727007230295122 1101 50-51 -1.0370711683538545 1101 52-53 -1.8912860220740129 1101 54-55 -1.9728211254019996 1101 56-57 -1.9587766296601004 1101 58-59 -2.068417711937343 1101 60-61 -1.5237416586125896 1101 62-63 -1.582403063511773 1101 64-65 -1.0161193633878192 1101 66-67 -1.9031564037986328 1101 68-69 -1.3393890925840246 1101 70-71 -0.9668312021626555 1101 72-73 -0.7140785391418056 1101 74-75 1.340889770192959 1101 76-77 0.5444008958350004 1101 78-79 1.1536451873230327 1101 80-81 0.40132183023007073 1101 82-83 -1.046577590508381 1101 84-85 0.5047867535118833 1101 86-87 0.7188031160567441 1101 88-89 1.5205887008344696 1101 90-91 1.0892426848519676 1101 92-93 1.8594524114629074 1101 94-95 0.05197751540023532 1101 96-97 0.6501209690100218 1101 98-99 0.5062865580609817 1101 100-101 -1.075756254875909 1102 1 -0.45743808803957364 1102 2 -0.13152893280418 1102 3 -0.43365638960559494 1102 4 -0.34031899022107126 1102 5 -0.597195532992437 1102 6 -0.8903074727822258 1102 7 -0.4316480450654936 1102 8 -0.3264394187390067 1102 9 -0.260868878303377 1102 10-11 0.1714370593326251 1102 12-13 -0.05074889509715064 1102 14-15 0.3574451616613814 1102 16-17 -0.1964051126638111 1102 18-19 -0.4310866065696146 1102 20-21 0.3723317511480104 1102 22-23 0.04418188842342374 1102 24-25 0.1819191626997636 1102 26-27 -0.5271981156656409 1102 28-29 -0.8127474319257857 1102 30-31 0.12859737764916446 1102 32-33 0.45573360327518486 1102 34-35 -0.40918359415110217 1102 36-37 0.33598446558698924 1102 38-39 0.48896639182775914 1102 40-41 0.5574896672643135 1102 42-43 -1.081194010770055 1102 44-45 -0.6042283897163259 1102 46-47 -0.3194721163894556 1102 48-49 -0.836382230295122 1102 50-51 -0.5214461683538545 1102 52-53 0.7024639779259871 1102 54-55 0.7615538745980004 1102 56-57 1.4005983703398996 1102 58-59 3.025332288062657 1102 60-61 2.8356333413874104 1102 62-63 3.011346936488227 1102 64-65 4.030755636612181 1102 66-67 2.753093596201367 1102 68-69 3.4418609074159754 1102 70-71 4.5956687978373445 1102 72-73 4.739046460858194 1102 74-75 4.372139770192959 1102 76-77 5.138150895835 1102 78-79 4.294270187323033 1102 80-81 3.1513218302300707 1102 82-83 4.453422409491619 1102 84-85 4.707911753511883 1102 86-87 4.921928116056744 1102 88-89 3.9268387008344696 1102 90-91 3.7454926848519676 1102 92-93 4.046952411462907 1102 94-95 3.7863525154002353 1102 96-97 4.525120969010022 1102 98-99 4.771911558060982 1102 100-101 4.392993745124091 1103 1 0.2284171751183237 1103 2 -0.0032394591199675915 1103 3 0.01700150513124754 1103 4 -0.0672926744315987 1103 5 0.3255018354286179 1103 6 0.22482410616514414 1103 7 0.01900984967134889 1103 8 0.02060005494520567 1103 9 -0.07336887830337702 1103 10-11 -0.01770767750947755 1103 12-13 0.01750768385021928 1103 14-15 0.36566884587190884 1103 16-17 0.23369357154671633 1103 18-19 0.2802620776409057 1103 20-21 0.40851596167432547 1103 22-23 0.37888583579184854 1103 24-25 0.29869547848923617 1103 26-27 0.6019137264396193 1103 28-29 0.1814959891268444 1103 30-31 0.709189482912322 1103 32-33 1.0947138664330751 1103 34-35 1.6220664058488978 1103 36-37 1.5423989392712016 1103 38-39 1.338472970775129 1103 40-41 1.9867659830537931 1103 42-43 1.4722599365983697 1103 44-45 2.6219229260731467 1103 46-47 2.7413831467684346 1103 48-49 3.0608217170733028 1103 50-51 3.69401435796194 1103 52-53 4.355424504241782 1103 54-55 3.6299749272295756 1103 56-57 4.46721021244516 1103 58-59 4.340299393325822 1103 60-61 4.000929394018986 1103 62-63 4.928287725961912 1103 64-65 4.775821426085866 1103 66-67 4.873981754096107 1103 68-69 4.791367486363345 1103 70-71 5.1269187978373445 1103 72-73 5.2957898819108244 1103 74-75 5.096646349140329 1103 76-77 4.6373285274139455 1103 78-79 5.663513608375663 1103 80-81 6.365959988124811 1103 82-83 5.7848368831758314 1103 84-85 5.847714385090832 1103 86-87 5.110250484477799 1103 88-89 4.839667648202891 1103 90-91 4.854867684851968 1103 92-93 4.832314253568171 1103 94-95 5.657240673294975 1103 96-97 5.854068337431073 1103 98-99 5.455299715955718 1103 100-101 5.21453979775567 1104 1 0.10426062990914176 1104 2 0.1296890159137689 1104 3 -0.02820767165687954 1104 4 0.10679639439431554 1104 5 0.054246774699869604 1104 6 0.21065406567931433 1104 7 -0.03901983993728919 1104 8 -0.11570223925182432 1104 9 -0.07336887830337702 1104 10-11 0.1850588542044207 1104 12-13 -0.060764920738172634 1104 14-15 -0.0648304793642609 1104 16-17 0.1429378360541378 1104 18-19 -0.15023724759525692 1104 20-21 -0.38568106936480717 1104 22-23 0.02294791406444574 1104 24-25 -0.1297795552489518 1104 26-27 -0.1325667054092321 1104 28-29 -0.5351032011565593 1104 30-31 -1.3225244172226311 1104 32-33 -1.0614939608273843 1104 34-35 -1.4035746197921242 1104 36-37 -1.6836469446694196 1104 38-39 -0.5474919415055695 1104 40-41 -0.8968372558126063 1104 42-43 -0.8388061902572375 1104 44-45 -0.26809056920350827 1104 46-47 -0.5045682702356089 1104 48-49 -0.7566546661925599 1104 50-51 -1.432503860661548 1104 52-53 -1.918529611817604 1104 54-55 -2.3987025356584084 1104 56-57 -1.6390650911985603 1104 58-59 -0.627311942706573 1104 60-61 0.3953288542079214 1104 62-63 -0.45700242248613065 1104 64-65 -1.3338276967211549 1104 66-67 -0.8146147371319685 1104 68-69 -0.12023845155838231 1104 70-71 0.6397393106578555 1104 72-73 0.11765223008896086 1104 74-75 -0.67393394775576 1104 76-77 -0.9055189759598719 1104 78-79 -0.617188146010303 1104 80-81 -1.2905852210519804 1104 82-83 -1.543773103328892 1104 84-85 -0.6566715798214524 1104 86-87 -0.8853635506099202 1104 88-89 -0.4521677094219392 1104 90-91 -1.0489784689941857 1104 92-93 -1.1201148962293992 1104 94-95 -0.6467404333177136 1104 96-97 -0.7889815950925403 1104 98-99 -0.6371429291185073 1104 100-101 -2.269265870260522 1105 1 0.10853413418264779 1105 2 0.20874884497359858 1105 3 0.36495472150551933 1105 4 0.4444032320011502 1105 5 0.21877668922978444 1105 6 0.3666369716622171 1105 7 0.3391852882678421 1105 8 0.5659216923721075 1105 9 0.676631121696623 1105 10-11 0.41796483711040366 1105 12-13 0.46487610490284936 1105 14-15 0.4511951616613814 1105 16-17 0.874775442891746 1105 18-19 0.8380106156526139 1105 20-21 0.7264984178146818 1105 22-23 0.4000846662012023 1105 24-25 0.36594694047754217 1105 26-27 0.9571768843343591 1105 28-29 0.7896831236297714 1105 30-31 1.0140140443158359 1105 32-33 1.183164158830742 1105 34-35 -0.3918224830399879 1105 36-37 -1.1483905344130108 1105 38-39 -1.2558252748389052 1105 40-41 -0.3904269994023579 1105 42-43 -0.32598567743671936 1105 44-45 0.30028549917255987 1105 46-47 -0.6701665608338985 1105 48-49 -0.2652016747395649 1105 50-51 0.23202605386837405 1105 52-53 0.61565842237043 1105 54-55 0.6782205412646718 1105 56-57 2.378028925895457 1105 58-59 3.7979017325071 1105 60-61 2.6880638969429675 1105 62-63 2.7908608253771128 1105 64-65 3.541172303278845 1105 66-67 4.157607485090253 1105 68-69 4.3220692407493075 1105 70-71 3.0713632422817874 1105 72-73 4.640088127524859 1105 74-75 4.988459214637402 1105 76-77 3.674609229168336 1105 78-79 4.313367409545254 1105 80-81 4.691252385785628 1105 82-83 3.8301585206027333 1105 84-85 4.287772864622994 1105 86-87 4.795192004945633 1105 88-89 3.8886442563900268 1105 90-91 4.646534351518632 1105 92-93 4.604244078129572 1105 94-95 5.399199737622457 1105 96-97 5.0112320801211325 1105 98-99 4.461147669172092 1105 100-101 4.540563189568534 1106 1 0.16679971683847583 1106 2 -0.06293137182856867 1106 3 0.08311190307733085 1106 4 0.01486393660819374 1106 5 0.06362763773927327 1106 6 -0.20585625327002788 1106 7 0.12170561347109299 1106 8 0.3389569227244067 1106 9 0.21931404852588798 1106 10-11 0.3414065715277488 1106 12-13 0.5502419585613865 1106 14-15 0.5396097958077277 1106 16-17 0.38934183855570126 1106 18-19 0.4054225397718483 1106 20-21 0.10556345846508464 1106 22-23 -0.014888233527798889 1106 24-25 0.44182769928512755 1106 26-27 0.23766468921240858 1106 28-29 0.0633958607571401 1106 30-31 -0.3813111589362066 1106 32-33 -0.24129383574921093 1106 34-35 0.34157860097084836 1106 36-37 0.6412436119284521 1106 38-39 0.5663291967058086 1106 40-41 0.13599576482528875 1106 42-43 -0.07395315711151795 1106 44-45 0.41139661028367414 1106 46-47 0.3565949567812723 1106 48-49 -0.0524645473682952 1106 50-51 0.6622428560363929 1106 52-53 1.6712139779259871 1106 54-55 2.145700216061421 1106 56-57 1.7614977605838007 1106 58-59 1.5058963124529043 1106 60-61 2.041044926753262 1106 62-63 1.4507524242931034 1106 64-65 1.597066612221937 1106 66-67 2.7382307913233177 1106 68-69 2.275321273269636 1106 70-71 1.4317968466178321 1106 72-73 1.8240312169557562 1106 74-75 1.4586489165344219 1106 76-77 0.6126173592496329 1106 78-79 1.338096406835227 1106 80-81 0.7606968302300707 1106 82-83 1.7129498485160077 1106 84-85 0.8230032169265193 1106 86-87 1.256912872154306 1106 88-89 1.1166252862003212 1106 90-91 2.0926725629007485 1106 92-93 1.492455460243395 1106 94-95 1.8995384910099915 1106 96-97 1.5556087738880713 1106 98-99 2.6793048507439075 1106 100-101 2.468069964636289 1107 1 0.4107021558628645 1107 2 0.27853204280557264 1107 3 0.44896556161391743 1107 4 0.42949808294966374 1107 5 0.453871540178298 1107 6 0.5990217955104598 1107 7 0.6582909793247538 1107 8 0.5340788739439191 1107 9 0.5607774631600364 1107 10-11 0.6340894983570138 1107 12-13 0.6721931780735773 1107 14-15 0.7713171128808867 1107 16-17 0.779585740994726 1107 18-19 0.502983515381608 1107 20-21 0.7397097999284981 1107 22-23 0.5948678640331764 1107 24-25 0.24670574806561518 1107 26-27 0.4571768843343591 1107 28-29 0.478030007098603 1107 30-31 -0.1374087199118108 1107 32-33 -0.1071474942857904 1107 34-35 -0.37793359415110217 1107 36-37 -1.0660734612422829 1107 38-39 -1.2141586081722409 1107 40-41 -1.107906674199107 1107 42-43 -1.0495629132090798 1107 44-45 -1.7349448531309548 1107 46-47 -1.9116977261455546 1107 48-49 -0.467098693709751 1107 50-51 -0.17922055859775554 1107 52-53 -0.36537138792766655 1107 54-55 -0.07381197906053671 1107 56-57 0.1395465410716028 1107 58-59 -0.07946954120563632 1107 60-61 -0.9833453171491762 1107 62-63 -0.9760768439995751 1107 64-65 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0.04099935137900701 2313 26-27 -0.5239551911373397 2313 28-29 -1.048891299850311 2313 30-31 0.04781907576236932 2313 32-33 -0.6955046042719815 2313 34-35 -1.274160009245442 2313 36-37 -1.3960320438469722 2313 38-39 -2.2613284194929975 2313 40-41 -2.8133829742451226 2313 42-43 -2.9329039164304334 2313 44-45 -1.7961505595276464 2313 46-47 -2.5771372107290773 2313 48-49 -1.566040249163045 2313 50-51 -2.457177300429329 2313 52-53 -3.0552011164136346 2313 54-55 -3.4695782008736984 2313 56-57 -2.5643190824902895 2313 58-59 -3.8871087496731924 2313 60-61 -3.6835293944616474 2313 62-63 -4.513122403134414 2313 64-65 -5.00992832565197 2313 66-67 -4.13222480002505 2313 68-69 -5.176358432206666 2313 70-71 -3.995722711596617 2313 72-73 -4.425163444802184 2313 74-75 -4.191834286410817 2313 76-77 -3.326176934353679 2313 78-79 -2.831024623997724 2313 80-81 -2.8430767546755895 2313 82-83 -1.4083110810744195 2313 84-85 -2.030885416299437 2313 86-87 -2.463979902811179 2313 88-89 -2.673986770863646 2313 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-0.29753062702774713 2314 48-49 -0.02288488986959436 2314 50-51 -0.03806850877938217 2314 52-53 0.820150148138751 2314 54-55 1.149851746938431 2314 56-57 1.1203457107654273 2314 58-59 1.4970743093392542 2314 60-61 0.8718700435150701 2314 62-63 0.6253761918073728 2314 64-65 0.3824843600164378 2314 66-67 1.62510157492477 2314 68-69 0.5791614393308713 2314 70-71 0.1694719893267056 2314 72-73 -0.3005147093545695 2314 74-75 0.2674190255121083 2314 76-77 0.14712695966478861 2314 78-79 -0.19874842969824513 2314 80-81 -0.2712180633869501 2314 82-83 1.0009623031086399 2314 84-85 -0.13184888478598822 2314 86-87 -1.0910373094751726 2314 88-89 -0.8504219374634054 2314 90-91 0.01976130187324543 2314 92-93 0.08119443273950466 2314 94-95 0.3126158132725756 2314 96-97 -1.2461556267346587 2314 98-99 -0.7669847185347614 2314 100-101 0.5056932132091951 2315 1 -0.5156488723532959 2315 2 -1.1915779524120218 2315 3 -0.7069407033310853 2315 4 -0.10134840198577422 2315 5 -0.547563180051263 2315 6 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1.9696989883582319 2315 68-69 1.6701084564355817 2315 70-71 2.930840366464796 2315 72-73 3.917048911838588 2315 74-75 3.573426534898843 2315 76-77 3.2898053075997034 2315 78-79 3.412223618695581 2315 80-81 2.662657614543793 2315 82-83 3.3121846643935804 2315 84-85 2.50019116527659 2315 86-87 2.1538521356645894 2315 88-89 3.3502455635795663 2315 90-91 2.8695735672049096 2315 92-93 4.079734274208004 2315 94-95 4.29217359383161 2315 96-97 4.765317047441396 2315 98-99 4.820624793355098 2315 100-101 4.365726588261346 2316 1 0.14041027261616534 2316 2 -0.03214368690254332 2316 3 -0.057631799441658416 2316 4 0.2959515015822092 2316 5 0.12360364733542895 2316 6 0.27874990426695234 2316 7 -0.11300050408188866 2316 8 0.1966138599495153 2316 9 -0.024188550434523393 2316 10-11 -0.04423917017557244 2316 12-13 0.259958072115964 2316 14-15 0.14176893215318387 2316 16-17 0.05487562504110599 2316 18-19 -0.01740832788109259 2316 20-21 0.1643399478693226 2316 22-23 0.3661593474398188 2316 24-25 0.3668576872899294 2316 26-27 0.2112752449900981 2316 28-29 0.34068494512339953 2316 30-31 0.3493965579770375 2316 32-33 0.002608603275184862 2316 34-35 0.023705750111190582 2316 36-37 -0.06847250162612539 2316 38-39 0.6383004082211983 2316 40-41 1.1164036016905428 2316 42-43 1.2505170548037157 2316 44-45 0.14910152831646428 2316 46-47 0.4853434573810347 2316 48-49 -0.3043637876721661 2316 50-51 -0.33036010278008376 2316 52-53 -1.2714089728936813 2316 54-55 -0.6359871090085605 2316 56-57 -0.24310040015190282 2316 58-59 -0.6752312365275088 2316 60-61 -0.7896228061535702 2316 62-63 -1.45868380121669 2316 64-65 -1.4759041994533924 2316 66-67 -0.4321010759297792 2316 68-69 -0.5973296663545149 2316 70-71 -0.8976713660970823 2316 72-73 -0.9302670637319714 2316 74-75 -0.1609032625939264 2316 76-77 -9.372189190983704E-4 2316 78-79 -0.24287120611959168 2316 80-81 -0.7229097271469804 2316 82-83 0.09199822916374956 2316 84-85 0.8651863436758198 2316 86-87 0.12556541113871234 2316 88-89 -0.6484686762147085 2316 90-91 -0.5754601840004909 2316 92-93 0.1629872475284806 2316 94-95 -0.5750716649276306 2316 96-97 0.4882357231083816 2316 98-99 0.3664300006839305 2316 100-101 0.14120071233720566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 9.0 15 47.0 16 103.0 17 300.0 18 499.0 19 625.0 20 816.0 21 1198.0 22 1445.0 23 1232.0 24 1038.0 25 1333.0 26 2082.0 27 1871.0 28 1953.0 29 2095.0 30 1156.0 31 605.0 32 545.0 33 808.0 34 1081.0 35 1987.0 36 4093.0 37 7545.0 38 6651.0 39 1053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.03942431007457 17.353821308127106 15.494228850995107 27.11252553080321 2 15.136352857481622 17.41522409295708 40.98411192791084 26.464311121650464 3 15.354517429452216 26.56153663741997 37.2018022290728 20.882143704055014 4 10.410244249466444 22.494664453402894 26.798672041735834 40.29641925539483 5 11.81645719705952 33.19895660422101 35.119753379179514 19.864832819539956 6 28.261797486364713 36.55916528337681 15.205122124733222 19.973915105525254 7 21.70974626511738 36.0777804126156 15.503912734171212 26.708560588095803 8 28.06023239269623 31.280531183305666 19.324164097699786 21.335072326298317 9 23.179985771875742 11.600663979132085 14.273179985771876 50.9461702632203 10-11 22.633388664927672 24.732036993123074 23.8819065686507 28.75266777329855 12-13 26.652833768081575 23.21792743656628 23.32582404553 26.803414749822146 14-15 25.179037230258476 23.86175005928385 21.73227412852739 29.226938581930284 16-17 22.90016599478302 30.743419492530233 21.622006165520514 24.73440834716623 18-19 21.710931942138963 21.23666113350723 27.717571733459806 29.334835190893997 20-21 20.397201802229073 19.200853687455538 30.889257766184492 29.5126867441309 22-23 18.819065686506995 24.083471662319184 31.185677021579323 25.911785629594497 24-25 20.26084894474745 26.861512923879534 31.697889494901588 21.179748636471423 26-27 19.656153663741996 26.382499407161493 31.84965615366374 22.11169077543277 28-29 16.113350723262982 19.724922930993596 33.96727531420441 30.19445103153901 30-31 17.3108845150581 20.120939056201088 35.9177140147024 26.65046241403842 32-33 17.896608963718283 22.174531657576477 37.34171211761916 22.58714726108608 34-35 17.20535926013754 26.51885226464311 37.69860090111454 18.577187574104816 36-37 19.47948778752668 24.082285985297606 29.422575290490872 27.01565093668485 38-39 20.903485890443445 20.73630543040076 33.26416884040787 25.096039838747924 40-41 17.978420678207257 20.463599715437514 36.287645245435144 25.27033436092009 42-43 21.26774090278519 19.342178588790475 37.9871708225139 21.40290968591043 44-45 17.751956367085604 22.806497510078255 36.268674413089876 23.172871709746264 46-47 22.997391510552525 19.48897320369931 30.28456248517904 27.229072800569128 48-49 21.197785129062474 19.7654703043669 33.11753755676496 25.919207009805667 50-51 19.991700260848944 24.40834716623192 31.386056438226227 24.21389613469291 52-53 18.047189945458857 27.532606118093433 33.63884277922694 20.781361157220772 54-55 17.832582404552998 28.162200616552052 28.8131373013991 25.192079677495848 56-57 21.303059046715674 30.496798672041734 23.81432297842068 24.38581930282191 58-59 16.753616314915817 32.37609675124496 29.98814322978421 20.882143704055014 60-61 20.722077306141806 35.60943798909177 22.679630068769267 20.988854635997154 62-63 17.00853687455537 38.477590704292155 22.871709746265118 21.64216267488736 64-65 16.863884277922693 37.60967512449609 23.07801754801992 22.4484230495613 66-67 16.932653545174293 36.885226464311124 23.120701920796773 23.06141806971781 68-69 17.35851741187351 35.09171320504156 23.74820664224143 23.8015627408435 70-71 18.14085843016362 33.493004505572685 23.9506758359023 24.415461228361394 72-73 17.99146312544463 31.578136115722078 24.414275551339813 26.01612520749348 74-75 17.839696466682476 30.08774009959687 24.96680104339578 27.105762390324877 76-77 18.27128290253735 29.673938819065686 24.777092719943088 27.27768555845388 78-79 18.6755987668959 29.11311358785867 24.91463125444629 27.296656390799146 80-81 18.680341474982214 28.963718283139674 24.14038415935499 28.21555608252312 82-83 18.465733934076358 28.18354280294048 24.63718283139673 28.713540431586438 84-85 18.906805786103863 27.224330092482806 24.659710694806737 29.209153426606594 86-87 19.290965141095565 27.159117856295943 24.219824519800806 29.330092482807686 88-89 19.4439174768793 26.764287408110032 23.92103391036282 29.870761204647856 90-91 19.61702632202988 26.041024424946645 24.075171923168128 30.266777329855348 92-93 19.484230495612994 25.135167180460044 24.185439886175004 31.195162437751954 94-95 19.343134930045057 25.040313018733695 24.61228361394356 31.004268437277688 96-97 19.306378942376096 25.015413801280534 24.866018496561537 30.812188759781833 98-99 19.255394830448186 25.367559876689587 24.54588570073512 30.831159592127104 100-101 19.415461228361394 25.51102679630069 24.175954470002374 30.89755750533555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21.0 1 13.5 2 14.5 3 17.5 4 12.5 5 15.5 6 19.5 7 39.5 8 72.5 9 94.0 10 88.5 11 74.0 12 72.0 13 77.5 14 84.5 15 94.0 16 108.5 17 113.5 18 116.0 19 126.0 20 138.5 21 152.5 22 175.0 23 195.5 24 212.0 25 235.0 26 252.0 27 286.0 28 348.0 29 397.5 30 442.5 31 493.5 32 546.0 33 604.0 34 657.5 35 755.0 36 910.0 37 1053.5 38 1154.5 39 1267.0 40 1345.0 41 1363.0 42 1384.0 43 1390.5 44 1336.5 45 1288.5 46 1297.5 47 1284.5 48 1278.0 49 1382.5 50 1589.0 51 1562.5 52 1220.0 53 941.5 54 859.0 55 831.5 56 785.0 57 822.5 58 883.0 59 895.0 60 918.0 61 937.0 62 902.0 63 803.5 64 629.0 65 476.5 66 420.0 67 373.0 68 319.5 69 269.0 70 204.0 71 141.0 72 118.5 73 90.0 74 71.0 75 66.0 76 53.0 77 39.5 78 24.5 79 14.5 80 6.0 81 3.0 82 4.0 83 3.5 84 2.0 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1517666587621532 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0011856770215793219 44-45 0.0 46-47 0.0 48-49 0.0011856770215793219 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0011856770215793219 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 42170.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.57647616789187 #Duplication Level Percentage of deduplicated Percentage of total 1 94.60995894304664 63.93407635760019 2 3.5828332806962133 4.842304956129951 3 0.7895567954521528 1.6006639791320845 4 0.2702038811102923 0.7303770452928622 5 0.2035301961609994 0.6876926725160066 6 0.10527423939362038 0.42684372776855584 7 0.06316454363617223 0.2987906094379891 8 0.049127978383689506 0.26559165283376807 9 0.03860055444432747 0.23476405027270572 >10 0.22809418535284418 3.0377045292862226 >50 0.021054847878724078 0.9532843253497748 >100 0.014036565252482718 2.7033436092008536 >500 0.0175457065656034 9.096514109556557 >1k 0.007018282626241359 11.188048375622481 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2859 6.779701209390562 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 1859 4.408347166231918 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 966 2.29072800569125 RNA PCR Primer, Index 39 (96% over 25bp) ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 961 2.2788712354754566 RNA PCR Primer, Index 39 (95% over 23bp) CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 683 1.6196348114773538 RNA PCR Primer, Index 39 (96% over 30bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 623 1.477353568887835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 1.429926488024662 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 462 1.0955655679392935 RNA PCR Primer, Index 39 (96% over 28bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.758833293810766 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 211 0.5003557031064738 Illumina PCR Primer Index 11 (96% over 25bp) ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC 147 0.3485890443443206 Illumina PCR Primer Index 11 (96% over 26bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.21342186388427792 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC 81 0.19207967749585012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65 0.15413801280531184 No Hit TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 62 0.1470239506758359 No Hit TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT 52 0.12331041024424946 RNA PCR Primer, Index 39 (95% over 22bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52 0.12331041024424946 No Hit ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT 45 0.10671093194213896 Illumina PCR Primer Index 11 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0035570310647379654 0.0 22-23 0.0 0.0 0.0 0.021342186388427793 0.0 24-25 0.0 0.0 0.0 0.11856770215793219 0.0 26-27 0.0 0.0 0.0 0.22883566516480913 0.0 28-29 0.0 0.0 0.0 0.4292150818117145 0.0 30-31 0.0 0.0 0.0 0.7695043870049798 0.0 32-33 0.0 0.0 0.0 1.1050509841119278 0.0 34-35 0.0 0.0 0.0 1.5935499170026084 0.0 36-37 0.0 0.0 0.0 2.274128527389139 0.0 38-39 0.0 0.0 0.0 3.0080626037467395 0.0 40-41 0.0 0.0 0.0 4.10718520275077 0.0 42-43 0.0 0.0 0.0 4.7106948067346455 0.0 44-45 0.0 0.0 0.0 5.109082285985298 0.0 46-47 0.0 0.0 0.0 5.544225752904909 0.0 48-49 0.0 0.0 0.0 6.014939530471899 0.0 50-51 0.0 0.0 0.0 6.420441071852028 0.0 52-53 0.0 0.0 0.0 6.682475693621058 0.0 54-55 0.0 0.0 0.0 7.000237135404316 0.0 56-57 0.0 0.0 0.0 7.392696229547071 0.0 58-59 0.0 0.0 0.0 7.69267251600664 0.0 60-61 0.0 0.0 0.0 7.947593075646194 0.0 62-63 0.0 0.0 0.0 8.175243063789424 0.0 64-65 0.0 0.0 0.0 8.42542091534266 0.0 66-67 0.0 0.0 0.0 8.68864121413327 0.0 68-69 0.0 0.0 0.0 8.992174531657577 0.0 70-71 0.0 0.0 0.0 9.210339103628172 0.0 72-73 0.0 0.0 0.0 9.416646905382974 0.0 74-75 0.0 0.0 0.0 9.63481147735357 0.0 76-77 0.0 0.0 0.0 9.879060943798908 0.0 78-79 0.0 0.0 0.0 10.10196822385582 0.0 80-81 0.0 0.0 0.0 10.361631491581694 0.0 82-83 0.0 0.0 0.0 10.626037467393882 0.0 84-85 0.0 0.0 0.0 10.870286933839221 0.0 86-87 0.0 0.0 0.0 11.201090822859854 0.0 88-89 0.0 0.0 0.0 11.589992885937871 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAGAA 25 0.004988117 56.999287 7 GGTATCA 420 0.0 49.82038 1 CTTATAC 815 0.0 36.177433 1 TTATACA 820 0.0 35.914185 2 ACATGGA 40 1.0659118E-5 35.624557 16-17 GTATCAA 595 0.0 35.125614 2 TATACAC 855 0.0 34.444016 3 CATGGGG 85 1.5955042E-5 33.52899 4 TACACAT 905 0.0 32.54103 5 ATACACA 910 0.0 32.362232 4 TATCAAC 675 0.0 30.962578 3 ATCAACG 685 0.0 30.51057 4 TCAACGC 685 0.0 30.51057 5 CAACGCA 700 0.0 29.856771 6 AACGCAG 670 0.0 29.775747 7 CACATCT 1010 0.0 29.158052 7 ACACATC 1025 0.0 28.731348 6 CGCAGAG 750 0.0 26.599669 9 CATCTCC 1110 0.0 26.531199 9 ACATGGG 215 5.2750693E-11 26.511297 3 >>END_MODULE