FastQCFastQC Report
Fri 10 Feb 2017
SRR2031815_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031815_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45112
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5321.179287107643199No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.7736300762546551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.7714133711651002No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.6650115268664657No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.58077673346338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.49432523497073944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.4012236212094343No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1250.2770881361943607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.25270438020925695No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.22832062422415322No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT890.1972867529703848No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT890.1972867529703848No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.1795531122539457No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.16625288171661642No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT680.15073594608973223No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG670.14851924100017733No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.1396524206419578No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT600.13300230537329313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.11748536974640893No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG490.10861854938818939No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC460.10196843411952475No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG250.00499013457.01
AGCTATT250.00499013457.08
CGCAGAA1050.054.2857139
TCTCTGA250.001607931938.020-21
CAGAAAA1100.036.70454410-11
TTATATA550.00255354334.5454562
ATGTAAA600.003911181431.6666681
ACCAGTC300.00393184531.66666854-55
ATAGTAT600.003911181431.6666686
CATGGGG650.00578550929.230774
GTGATGT650.00578550929.230779
AACACTG856.311496E-427.9411757
GTCATGC350.00835135127.14285958-59
ACTAGAG350.00835135127.14285978-79
CATAGGG350.00835135127.14285918-19
AATCTCT350.00835135127.14285918-19
GCCAAGA350.00835135127.14285984-85
TGGTCCA350.00835135127.14285910-11
GAGTACT2303.6379788E-1226.8478261
GGTATCA6300.026.3888871
Produced by FastQC (version 0.11.5)