##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031815_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45112 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56359726901933 34.0 31.0 34.0 30.0 34.0 2 31.65740822840929 34.0 31.0 34.0 30.0 34.0 3 31.679264940592304 34.0 31.0 34.0 28.0 34.0 4 34.851613761305195 37.0 35.0 37.0 32.0 37.0 5 34.891669622273454 37.0 35.0 37.0 32.0 37.0 6 35.033937754921084 37.0 36.0 37.0 32.0 37.0 7 35.06084855470828 37.0 36.0 37.0 33.0 37.0 8 35.08235059407696 37.0 37.0 37.0 33.0 37.0 9 36.785688951941836 39.0 38.0 39.0 33.0 39.0 10-11 36.818174765029255 39.0 38.0 39.0 33.0 39.0 12-13 36.79138588402199 39.0 38.0 39.0 33.0 39.0 14-15 38.21069781876219 41.0 39.0 41.0 33.5 41.0 16-17 38.118815392800144 41.0 39.0 41.0 33.0 41.0 18-19 38.12606401844299 41.0 39.0 41.0 33.5 41.0 20-21 38.071455488561796 41.0 39.0 41.0 33.5 41.0 22-23 37.903540078028016 41.0 38.5 41.0 33.0 41.0 24-25 37.83339244546905 41.0 38.0 41.0 33.0 41.0 26-27 37.6095938996276 40.5 37.5 41.0 32.5 41.0 28-29 37.36081308742685 40.0 37.0 41.0 32.0 41.0 30-31 37.12470074481291 40.0 36.0 41.0 31.0 41.0 32-33 36.9071089732222 40.0 35.0 41.0 31.0 41.0 34-35 36.613672636992376 40.0 35.0 41.0 30.0 41.0 36-37 36.417638322397586 40.0 35.0 41.0 29.5 41.0 38-39 36.106357510196844 40.0 35.0 41.0 27.0 41.0 40-41 35.86762945557723 40.0 35.0 41.0 25.0 41.0 42-43 35.56685582550098 40.0 35.0 41.0 23.5 41.0 44-45 35.49218611455932 40.0 35.0 41.0 20.5 41.0 46-47 35.16303865933676 40.0 35.0 41.0 16.5 41.0 48-49 34.825977566944495 40.0 35.0 41.0 13.0 41.0 50-51 33.777553644263165 38.5 34.0 40.0 11.0 40.5 52-53 33.80307900336939 38.5 34.5 39.5 10.0 40.5 54-55 34.45173124667494 39.5 35.0 41.0 10.0 41.0 56-57 34.42126263521901 40.0 35.0 41.0 10.0 41.0 58-59 34.243083880120594 39.0 35.0 41.0 10.0 41.0 60-61 33.99092259265828 39.0 35.0 41.0 10.0 41.0 62-63 33.69446045398121 39.0 34.5 41.0 9.5 41.0 64-65 33.339166962227345 37.5 34.0 41.0 8.0 41.0 66-67 32.99196444405037 37.0 34.0 40.0 8.0 41.0 68-69 32.57310693385352 36.5 33.0 39.5 8.0 41.0 70-71 32.19202207838269 36.0 33.0 39.0 8.0 41.0 72-73 31.756439528285156 35.5 32.5 39.0 7.5 41.0 74-75 31.34657075722646 35.0 32.0 37.0 7.0 39.5 76-77 30.99353830466395 35.0 32.0 37.0 7.0 39.0 78-79 30.631051604894484 35.0 31.0 36.5 7.0 39.0 80-81 30.303200922149315 35.0 31.0 36.0 7.0 38.0 82-83 30.03899184252527 35.0 31.0 36.0 7.0 37.0 84-85 29.760584766802623 35.0 30.0 35.0 7.0 37.0 86-87 29.55642622805462 35.0 30.0 35.0 7.0 36.0 88-89 29.33332594431637 35.0 29.0 35.0 7.0 36.0 90-91 29.160611367263698 35.0 29.0 35.0 7.0 36.0 92-93 29.04004477744281 35.0 29.0 35.0 7.0 36.0 94-95 28.846792427735416 35.0 29.0 35.0 7.0 35.0 96-97 28.75288171661642 35.0 27.5 35.0 7.0 35.0 98-99 28.62409115091328 35.0 27.0 35.0 7.0 35.0 100-101 27.249534491931193 33.0 23.0 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42810299321308776 1101 2 0.4116696906276225 1101 3 0.9847060928026927 1101 4 0.844528430642292 1101 5 0.8400873389264873 1101 6 1.0162057032717726 1101 7 0.976618055324991 1101 8 1.354032407519611 1101 9 0.6722982698028872 1101 10-11 1.0643119992997399 1101 12-13 0.6611043646867785 1101 14-15 0.9838360361469967 1101 16-17 0.9435682343579757 1101 18-19 1.4885494932078984 1101 20-21 1.1364025785969005 1101 22-23 1.1279236615721473 1101 24-25 1.4430173799080492 1101 26-27 1.2008238250539236 1101 28-29 1.5492481854791862 1101 30-31 1.3299948140175886 1101 32-33 1.0801921084370392 1101 34-35 1.7636671342897756 1101 36-37 1.9052484463809591 1101 38-39 1.656437014369061 1101 40-41 2.5905154021130627 1101 42-43 2.2048822363092526 1101 44-45 2.4417442329266876 1101 46-47 3.0355990209535193 1101 48-49 3.3267514674143612 1101 50-51 2.4903353580005785 1101 52-53 2.794508548367425 1101 54-55 2.7797751274895006 1101 56-57 2.392940366928393 1101 58-59 2.6591722073482558 1101 60-61 2.497236141203466 1101 62-63 2.903042469689197 1101 64-65 2.489717556071085 1101 66-67 2.2473813239706715 1101 68-69 1.6885820429574139 1101 70-71 2.0327536711953798 1101 72-73 2.0960658700854076 1101 74-75 2.7096554789836738 1101 76-77 2.7616118219524957 1101 78-79 2.4074006971422044 1101 80-81 1.9618584831544759 1101 82-83 2.1203783279057298 1101 84-85 1.5454385779422743 1101 86-87 1.3992059440871039 1101 88-89 1.67710279826289 1101 90-91 1.6747595722314337 1101 92-93 1.587242651170989 1101 94-95 1.3888327153343702 1101 96-97 0.6384150227581777 1101 98-99 0.5942465031164126 1101 100-101 0.7694232619392558 1102 1 -0.046897006786913664 1102 2 -0.07656560349002106 1102 3 0.27882373986151876 1102 4 0.10482254828934856 1102 5 0.15920498598531196 1102 6 -0.2632060614341114 1102 7 -0.2866172387926511 1102 8 -0.6547911218921598 1102 9 0.42671003450877265 1102 10-11 0.12534141106444707 1102 12-13 0.7449278940985451 1102 14-15 0.48383603614699666 1102 16-17 0.5009211755344438 1102 18-19 0.5709024343843652 1102 20-21 0.9180202256557237 1102 22-23 1.2882177792192024 1102 24-25 0.9533114975551058 1102 26-27 0.8934708838774554 1102 28-29 1.462483479596834 1102 30-31 1.2652889316646494 1102 32-33 0.7735744613782103 1102 34-35 1.4952847813486017 1102 36-37 1.803777858145672 1102 38-39 0.9409958378984769 1102 40-41 0.4228683432895295 1102 42-43 0.8056175304269004 1102 44-45 1.118950115279631 1102 46-47 1.3701578444829252 1102 48-49 1.6208691144731873 1102 50-51 0.7601882991770523 1102 52-53 1.186420313073306 1102 54-55 1.7180104216071541 1102 56-57 2.7172050728107493 1102 58-59 2.4194663249953123 1102 60-61 2.5273832000269962 1102 62-63 2.0934836461597897 1102 64-65 2.32795285018873 1102 66-67 2.373116618088318 1102 68-69 2.3312291017809486 1102 70-71 2.4371654359012638 1102 72-73 2.3887129289089337 1102 74-75 2.486126067218958 1102 76-77 2.0741118219524957 1102 78-79 1.6294595206716131 1102 80-81 1.5486231890368245 1102 82-83 1.0644959749645544 1102 84-85 1.569703283824623 1102 86-87 2.1683235911459313 1102 88-89 1.6851910335570075 1102 90-91 1.474024278113788 1102 92-93 1.6990073570533433 1102 94-95 1.4932444800402536 1102 96-97 2.03694443452288 1102 98-99 2.523658267822295 1102 100-101 2.0172173795863166 1103 1 -0.24836759502220573 1103 2 -0.029506779960609464 1103 3 -0.30941155425612976 1103 4 0.550410783583466 1103 5 0.19302851539708143 1103 6 -0.7485001790811694 1103 7 -0.0527937093808859 1103 8 -0.234202886598041 1103 9 1.1428865050970032 1103 10-11 -0.8474527065826081 1103 12-13 -0.36830740001910556 1103 14-15 -0.5455757285588874 1103 16-17 -0.11525529505378529 1103 18-19 0.2238436108549564 1103 20-21 -0.09889153905015746 1103 22-23 -0.3867822207807947 1103 24-25 0.1636056152021652 1103 26-27 0.49494147211274253 1103 28-29 -0.36251652040316884 1103 30-31 0.09470069637053058 1103 32-33 -0.3756902445041348 1103 34-35 -0.8686858068866883 1103 36-37 0.16995432873390115 1103 38-39 1.259378190839648 1103 40-41 1.1640448138777586 1103 42-43 0.4107645892504266 1103 44-45 1.1182148211619776 1103 46-47 0.6238343150711572 1103 48-49 1.0767514674143612 1103 50-51 1.8873941815299915 1103 52-53 1.2503909013085988 1103 54-55 1.0150692451365657 1103 56-57 0.7605874257519218 1103 58-59 0.4827016191129587 1103 60-61 1.4825302588505238 1103 62-63 0.932454234395081 1103 64-65 1.122070497247556 1103 66-67 1.8503225004412656 1103 68-69 1.585640866486834 1103 70-71 1.7827536711953798 1103 72-73 1.6254776347912845 1103 74-75 0.8125966554542536 1103 76-77 1.0116118219524957 1103 78-79 0.5838712853774943 1103 80-81 0.9471526008015303 1103 82-83 0.6644959749645558 1103 84-85 0.6630856367657998 1103 86-87 0.2962647676165169 1103 88-89 0.13298515120406407 1103 90-91 0.8953478075255532 1103 92-93 0.8372426511709854 1103 94-95 0.2564797741579028 1103 96-97 0.5942973756993517 1103 98-99 0.44718767958699956 1103 100-101 0.4017762031157268 1104 1 -0.5718970067869122 1104 2 0.02613709921267926 1104 3 -0.6671222060844286 1104 4 0.24198471045151138 1104 5 0.026096877877208158 1104 6 -0.85104389927195 1104 7 -0.2467523739277908 1104 8 0.47088455378352023 1104 9 0.6881965209952554 1104 10-11 0.16486843809148155 1104 12-13 0.5712792454499009 1104 14-15 0.38248468479564224 1104 16-17 1.0961914458047204 1104 18-19 0.38441594789787814 1104 20-21 0.5251148202503231 1104 22-23 0.815920481921907 1104 24-25 0.27290609214970374 1104 26-27 0.8049573703639368 1104 28-29 0.5699159120292663 1104 30-31 0.2747483911241062 1104 32-33 0.19823662354037452 1104 34-35 0.8212982948621175 1104 36-37 1.235534614902427 1104 38-39 1.8170093514119898 1104 40-41 0.9188142892354776 1104 42-43 0.4687932061025748 1104 44-45 0.07807173690125069 1104 46-47 -0.3173421555170748 1104 48-49 -0.3385903449862724 1104 50-51 -0.7421765656878208 1104 52-53 0.1414878806408737 1104 54-55 0.7193617729585071 1104 56-57 0.4195699376756181 1104 58-59 -0.3960742155452266 1104 60-61 0.12163995678375272 1104 62-63 -0.4872595970834581 1104 64-65 -0.36799309575721395 1104 66-67 -0.313032030560322 1104 68-69 1.1575804531323044 1104 70-71 0.47432759806342517 1104 72-73 -0.3403411251451196 1104 74-75 -0.2963063652134714 1104 76-77 -0.1235233131826412 1104 78-79 0.5882433044553927 1104 80-81 -0.07907951366587795 1104 82-83 -0.20712564665706523 1104 84-85 -0.022526445905103998 1104 86-87 0.15953980736214746 1104 88-89 -0.19014680428083253 1104 90-91 0.20138914297865185 1104 92-93 -0.4604521024061192 1104 94-95 0.7087850205807946 1104 96-97 -0.09886637628792982 1104 98-99 -0.43715254298851747 1104 100-101 -1.0831204582515248 1105 1 -1.2060433482503257 1105 2 -1.3351021888558776 1105 3 -0.7699567479433576 1105 4 -1.481153061466749 1105 5 -1.5731120871854216 1105 6 -0.9644255736292351 1105 7 -0.6725928485487529 1105 8 -0.7657667316482559 1105 9 -0.3592655752473277 1105 10-11 -0.8377683450331119 1105 12-13 -0.560559910779503 1105 14-15 -0.4247005492188549 1105 16-17 -0.18627394641677597 1105 18-19 -0.09251219976197689 1105 20-21 0.05247144516792446 1105 22-23 -0.10629441590274524 1105 24-25 0.19050661950632986 1105 26-27 -0.8034803356347453 1105 28-29 -0.6308092033299957 1105 30-31 -0.5139793610182792 1105 32-33 -0.5005109044754477 1105 34-35 -0.8873371698709107 1105 36-37 0.33243639473104025 1105 38-39 -0.46479684502835283 1105 40-41 -0.39298531524705993 1105 42-43 -1.6824922256706643 1105 44-45 -1.2996474456959817 1105 46-47 -1.7319763018585377 1105 48-49 -1.2205943001609612 1105 50-51 -1.9626775544814876 1105 52-53 -2.0724211503413272 1105 54-55 -2.2173554320513844 1105 56-57 -1.7867583418234005 1105 58-59 -0.8677287969559089 1105 60-61 -1.7082875316803197 1105 62-63 -2.231821231888997 1105 64-65 -2.4464373937137083 1105 66-67 -2.0668224063019203 1105 68-69 -2.9132830933410006 1105 70-71 -2.13905407629386 1105 72-73 -2.713116339383749 1105 74-75 -1.9858251522932306 1105 76-77 -2.207900373169455 1105 78-79 -2.329686820791803 1105 80-81 -1.498328030475374 1105 82-83 -1.8172113421086173 1105 84-85 -2.353772325931473 1105 86-87 -2.934725189341872 1105 88-89 -2.962065064003969 1105 90-91 -2.565914746276455 1105 92-93 -2.683919472214953 1105 94-95 -2.880536007764622 1105 96-97 -2.800250687428342 1105 98-99 -2.8824392931533147 1105 100-101 -1.9647948155356367 1106 1 0.7304285746084389 1106 2 1.0676204430216067 1106 3 1.023009786373148 1106 4 0.8705202227079525 1106 5 0.3435073115667109 1106 6 0.8240032408914715 1106 7 0.3534990402771143 1106 8 0.5393949246194722 1106 9 0.6435704996250493 1106 10-11 0.498306527343523 1106 12-13 0.6734162661915661 1106 14-15 0.6873244082400163 1106 16-17 1.0817351290228174 1106 18-19 0.6383442948494817 1106 20-21 0.8258690628650243 1106 22-23 0.7294968489866491 1106 24-25 1.5387766138341803 1106 26-27 1.1440522792262868 1106 28-29 1.2188788284340433 1106 30-31 1.3385447456181367 1106 32-33 1.1639814381223985 1106 34-35 1.1566219906509332 1106 36-37 0.9555220441921861 1106 38-39 1.524426070456613 1106 40-41 2.0310078781732486 1106 42-43 2.0721872978687585 1106 44-45 2.2936594176052054 1106 46-47 2.3687043561108325 1106 48-49 2.516217951682485 1106 50-51 2.215711554990996 1106 52-53 2.399501708422143 1106 54-55 2.833126700676914 1106 56-57 2.496565537927026 1106 58-59 3.330512836623221 1106 60-61 2.911394827933975 1106 62-63 2.9304022508109497 1106 64-65 2.845394710653842 1106 66-67 2.8588724320418137 1106 68-69 2.945763985501877 1106 70-71 2.777281714971025 1106 72-73 3.4346431614670756 1106 74-75 3.3570562997771027 1106 76-77 3.3720769382315687 1106 78-79 3.3277153346251005 1106 80-81 2.705018537874029 1106 82-83 2.7633331842668802 1106 84-85 3.5560404931269503 1106 86-87 2.6758817306808176 1106 88-89 3.4616445219290988 1106 90-91 3.1737335804393716 1106 92-93 2.150170147751016 1106 94-95 2.6915002939937445 1106 96-97 2.6305490856856686 1106 98-99 3.234123384101366 1106 100-101 2.8128569144700357 1107 1 0.028102993213089178 1107 2 -0.25156560349002177 1107 3 -0.24617626013847982 1107 4 -0.27017745171065144 1107 5 -0.21579501401468804 1107 6 0.13679393856588717 1107 7 -0.46161723879265537 1107 8 0.04520887810784302 1107 9 -0.32328996549122735 1107 10-11 -0.4621585889355444 1107 12-13 -0.6175721059014592 1107 14-15 -0.6036639638530019 1107 16-17 -0.6240788244655562 1107 18-19 -0.27909756561563626 1107 20-21 0.0680202256557294 1107 22-23 0.16321777921920244 1107 24-25 -0.009188502444892777 1107 26-27 -0.8565291161225446 1107 28-29 -0.100016520403166 1107 30-31 -0.3222110683353563 1107 32-33 -0.3764255386217883 1107 34-35 0.1327847813486045 1107 36-37 0.3912778581456706 1107 38-39 0.5284958378984754 1107 40-41 1.0603683432895252 1107 42-43 0.9806175304268976 1107 44-45 0.8939501152796296 1107 46-47 1.0951578444829195 1107 48-49 1.6583691144731887 1107 50-51 2.2601882991770523 1107 52-53 2.111420313073303 1107 54-55 1.8555104216071499 1107 56-57 1.7672050728107465 1107 58-59 1.5819663249953138 1107 60-61 0.7648832000269934 1107 62-63 0.13098364615978397 1107 64-65 0.21545285018873273 1107 66-67 0.2856166180883264 1107 68-69 0.2812291017809443 1107 70-71 0.012165435901266619 1107 72-73 0.6012129289089394 1107 74-75 1.0236260672189665 1107 76-77 1.049111821952497 1107 78-79 1.1919595206716096 1107 80-81 0.6361231890368266 1107 82-83 1.4144959749645558 1107 84-85 1.1322032838246265 1107 86-87 1.3933235911459292 1107 88-89 0.9601910335570061 1107 90-91 1.349024278113788 1107 92-93 0.9365073570533404 1107 94-95 0.24324448004025712 1107 96-97 1.3244444345228779 1107 98-99 1.136158267822296 1107 100-101 1.079717379586313 1108 1 0.42810299321308776 1108 2 0.8007071237827041 1108 3 0.46064192167969864 1108 4 0.05709527556207661 1108 5 0.5887504405307666 1108 6 0.9640666658386152 1108 7 0.6838373066618928 1108 8 0.9542997871987495 1108 9 1.2017100345087712 1108 10-11 0.5014777747008097 1108 12-13 0.8222006213712731 1108 14-15 0.7338360361469967 1108 16-17 1.09864844826172 1108 18-19 0.8368115252934558 1108 20-21 0.46461113474663307 1108 22-23 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-0.17656560349002248 2311 3 -0.3611762601384818 2311 4 -0.18017745171064803 2311 5 -0.5857950140146855 2311 6 0.1667939385658883 2311 7 -0.09161723879265082 2311 8 0.09520887810784018 2311 9 -1.1782899654912313 2311 10-11 -0.6121585889355501 2311 12-13 0.2899278940985468 2311 14-15 0.20383603614699553 2311 16-17 0.7459211755344484 2311 18-19 0.5109024343843629 2311 20-21 -0.13947977434427372 2311 22-23 0.553217779219203 2311 24-25 0.43831149755510523 2311 26-27 -0.22152911612254655 2311 28-29 -0.30251652040316657 2311 30-31 0.07528893166465167 2311 32-33 0.23607446137821597 2311 34-35 0.45778478134860734 2311 36-37 0.43877785814567005 2311 38-39 0.17349583789847145 2311 40-41 0.4128683432895244 2311 42-43 -0.2768824695731027 2311 44-45 0.3264501152796342 2311 46-47 0.4326578444829252 2311 48-49 0.8208691144731901 2311 50-51 1.242688299177054 2311 52-53 1.083920313073314 2311 54-55 0.7080104216071561 2311 56-57 0.604705072810745 2311 58-59 0.5344663249953143 2311 60-61 0.664883200026992 2311 62-63 1.2459836461597789 2311 64-65 0.39795285018873017 2311 66-67 1.3356166180883235 2311 68-69 1.326229101780946 2311 70-71 1.0621654359012638 2311 72-73 0.9037129289089343 2311 74-75 1.1261260672189586 2311 76-77 0.18161182195249737 2311 78-79 0.7044595206716089 2311 80-81 0.43362318903682606 2311 82-83 0.6844959749645589 2311 84-85 0.3972032838246271 2311 86-87 0.463323591145933 2311 88-89 0.6976910335570068 2311 90-91 0.49652427811378885 2311 92-93 1.149007357053339 2311 94-95 0.9082444800402563 2311 96-97 0.7619444345228779 2311 98-99 0.3236582678222959 2311 100-101 0.6647173795863139 2312 1 0.7799548450649425 2312 2 0.6938047668803513 2312 3 0.8343792954170759 2312 4 0.4131558816226857 2312 5 -0.7546839029035723 2312 6 0.19049764226959098 2312 7 -0.16717279434821108 2312 8 0.5915051744041406 2312 9 0.18319151599025219 2312 10-11 0.7211747443977785 2312 12-13 0.6088167829874322 2312 14-15 0.22457677688773714 2312 16-17 0.1314767310900038 2312 18-19 0.8801616936436218 2312 20-21 0.4120017071372075 2312 22-23 0.3354400014414267 2312 24-25 0.19683001607362627 2312 26-27 0.06847088387745259 2312 28-29 -0.5199239278105736 2312 30-31 0.0838074501831656 2312 32-33 0.9712596465634036 2312 34-35 0.6688958924597159 2312 36-37 1.0991482285160359 2312 38-39 -0.4557634213607855 2312 40-41 0.5839794544006409 2312 42-43 1.2931175304268976 2312 44-45 1.6971908560203701 2312 46-47 1.4141393259644062 2312 48-49 1.3616098552139277 2312 50-51 1.650466076954828 2312 52-53 1.9720684612214612 2312 54-55 1.571714125310855 2312 56-57 1.341742109847786 2312 58-59 1.9037255842545733 2312 60-61 2.513031348175147 2312 62-63 1.9226503128264483 2312 64-65 0.7446195168554013 2312 66-67 1.002283284754995 2312 68-69 0.4914142869661333 2312 70-71 0.7936469173827447 2312 72-73 1.3711203363163413 2312 74-75 1.284274215367109 2312 76-77 1.5856858960265718 2312 78-79 1.600755816967908 2312 80-81 1.3643639297775643 2312 82-83 1.7478293082978915 2312 84-85 1.5683143949357365 2312 86-87 2.0451754429977846 2312 88-89 1.860653996519968 2312 90-91 1.18837612996564 2312 92-93 1.4527110607570428 2312 94-95 1.7886148504106245 2312 96-97 1.304537027115476 2312 98-99 1.540324934488961 2312 100-101 1.5510136758826114 2313 1 -0.4532529389903033 2313 2 -0.3545317051849395 2313 3 -0.7042271075961075 2313 4 -1.326957112727598 2313 5 -0.15393060723502572 2313 6 -0.09795182414597292 2313 7 0.05787428663107619 2313 8 -1.2895368846040185 2313 9 -1.2728662366776717 2313 10-11 -0.07402299571521098 2313 12-13 -0.05973312285060217 2313 14-15 -0.2915876926665675 2313 16-17 -0.32662119734690975 2313 18-19 -0.9172331588359768 2313 20-21 -1.2050729946832561 2313 22-23 -1.9715279834926562 2313 24-25 -1.01660375668218 2313 26-27 0.14897935845372245 2313 28-29 0.06121229315615295 2313 30-31 -0.46437208528450213 2313 32-33 -0.0571458776048388 2313 34-35 -0.3617067440751285 2313 36-37 -1.0551204469390782 2313 38-39 -1.2812499248133875 2313 40-41 -1.8915384363714907 2313 42-43 -1.1136621305900505 2313 44-45 -1.2638888677712217 2313 46-47 -0.5207319860255453 2313 48-49 0.4937504704053879 2313 50-51 -0.046803226246673546 2313 52-53 -3.8837068055707604 2313 54-55 -2.8099556800877643 2313 56-57 -0.9452949271892521 2313 58-59 -0.21146587839451314 2313 60-61 0.5936967593490294 2313 62-63 -0.006728218246998097 2313 64-65 -0.057640370150252807 2313 66-67 -0.7067562632676072 2313 68-69 -0.5836014066936244 2313 70-71 0.06640272403685543 2313 72-73 -1.752388766006316 2313 74-75 -0.2876027463403581 2313 76-77 0.4522897880541912 2313 78-79 0.8722561308411017 2313 80-81 -0.41874969231910697 2313 82-83 0.1263603817442167 2313 84-85 0.23262701263818286 2313 86-87 -0.6329475952947483 2313 88-89 0.13921645728581922 2313 90-91 -0.1041536879879068 2313 92-93 -0.5433655243025903 2313 94-95 -0.5097216216546592 2313 96-97 0.5255037565567804 2313 98-99 -0.03312139319465146 2313 100-101 0.5428529728066529 2314 1 -0.15254216807723608 2314 2 0.04924084812288143 2314 3 -0.543756905299773 2314 4 0.28949996764418984 2314 5 -0.5432143688533984 2314 6 0.08356813211427294 2314 7 -0.07935917427652583 2314 8 -0.4305975735050609 2314 9 -1.0724835138783249 2314 10-11 -1.3944166534516782 2314 12-13 -1.2962011381595175 2314 14-15 -0.00809944772397131 2314 16-17 0.13237278843767797 2314 18-19 0.5160637247069388 2314 20-21 -1.1662539678926578 2314 22-23 -0.8383951240066025 2314 24-25 -0.28943043792876466 2314 26-27 0.25395475484519636 2314 28-29 0.07296735056457493 2314 30-31 -0.06374332639986591 2314 32-33 -0.9397319902346908 2314 34-35 -0.08737650897397486 2314 36-37 0.18845527750050906 2314 38-39 -1.0110202911337822 2314 40-41 0.13415866587016723 2314 42-43 0.17214978849141005 2314 44-45 -0.7451627879461782 2314 46-47 -2.422180865194491 2314 48-49 -2.1049373371397095 2314 50-51 -1.4660213782423028 2314 52-53 -0.7164022675718513 2314 54-55 -0.5190863525863989 2314 56-57 -0.3565852497698998 2314 58-59 -0.8152110943595261 2314 60-61 -0.6189877677149411 2314 62-63 -1.4375647409369918 2314 64-65 -0.9704342465854623 2314 66-67 -0.8821253173955483 2314 68-69 -0.935706382090018 2314 70-71 -0.8491248866793839 2314 72-73 -0.34273868399428764 2314 74-75 -0.7993578037487801 2314 76-77 -0.4077430167571805 2314 78-79 0.5383304884135462 2314 80-81 -1.6263768109631762 2314 82-83 -0.8193749927773801 2314 84-85 -1.768603167788278 2314 86-87 -0.8147409249831021 2314 88-89 -0.21521219224944588 2314 90-91 -0.1634757218862113 2314 92-93 0.1441686473759205 2314 94-95 0.587599318749934 2314 96-97 0.0183960474261049 2314 98-99 -0.029567538629315493 2314 100-101 0.18213673442502554 2315 1 0.49706851045446854 2315 2 -0.1593242241796773 2315 3 0.002961670896002744 2315 4 0.4246501344962468 2315 5 0.13420498598531339 2315 6 0.19369049029002383 2315 7 0.009072416379758863 2315 8 -0.7392738805128474 2315 9 -0.229324448249848 2315 10-11 -0.12077927859072446 2315 12-13 -1.219727278315247 2315 14-15 -0.32650879143920974 2315 16-17 0.32592117553444666 2315 18-19 -0.12909756561563768 2315 20-21 0.5253478118626234 2315 22-23 0.8287350205985149 2315 24-25 -0.01134367485868637 2315 26-27 -0.2763567023294442 2315 28-29 -0.45734410661006564 2315 30-31 0.5911510006301626 2315 32-33 -0.05875312482868367 2315 34-35 -0.9939393565824304 2315 36-37 -0.28846352116467955 2315 38-39 0.07073721720882276 2315 40-41 0.49873041225504267 2315 42-43 0.9827727028406912 2315 44-45 0.516794942865836 2315 46-47 0.6309337065518932 2315 48-49 0.638110493783536 2315 50-51 0.6399296784873982 2315 52-53 1.2118513475560704 2315 54-55 1.0921483526416367 2315 56-57 -0.1435707892582201 2315 58-59 -0.6055336750046862 2315 60-61 0.41660733795802685 2315 62-63 -0.20091290556435837 2315 64-65 0.681401126050801 2315 66-67 1.4649269629159107 2315 68-69 1.4872635845395692 2315 70-71 0.9845792290047086 2315 72-73 0.5326784461503173 2315 74-75 0.7050915844603445 2315 76-77 1.3133359598835277 2315 78-79 0.9165284861888523 2315 80-81 0.916726637312685 2315 82-83 1.9231166646197302 2315 84-85 1.612375697617729 2315 86-87 0.9950477290769619 2315 88-89 0.04424275769493491 2315 90-91 0.14686910569999512 2315 92-93 1.0334901156740308 2315 94-95 1.0820375834885319 2315 96-97 1.6515996069366743 2315 98-99 1.184003095408503 2315 100-101 1.3064415175173458 2316 1 0.03127759638769234 2316 2 0.6408947139702939 2316 3 -0.2926048315670542 2316 4 0.016330484797286715 2316 5 0.08658593836626238 2316 6 -0.18780923603728894 2316 7 0.06298593581052359 2316 8 0.3190184019173685 2316 9 0.43980527260401203 2316 10-11 -0.02882525560222149 2316 12-13 -0.04197686780621268 2316 14-15 -0.9883861860752248 2316 16-17 -0.5391581895449207 2316 18-19 -0.541795978314056 2316 20-21 0.18448847962397963 2316 22-23 -0.06932190332047838 2316 24-25 0.368787688031297 2316 26-27 0.2549788203853893 2316 28-29 -0.2831514410380933 2316 30-31 -0.5894729730972585 2316 32-33 0.16305858836233966 2316 34-35 -0.009675536111714678 2316 36-37 -0.29899991963210226 2316 38-39 -0.9980914636888301 2316 40-41 -0.5350681646469795 2316 42-43 -0.9767237394143748 2316 44-45 -1.1625975037679908 2316 46-47 -1.6189294571043789 2316 48-49 -2.585480091876015 2316 50-51 -1.6233434468546974 2316 52-53 -2.267349528196533 2316 54-55 -1.6478625942658596 2316 56-57 -1.4294219113162399 2316 58-59 -1.8079146273856352 2316 60-61 -1.9605136253698277 2316 62-63 -1.4662385760624375 2316 64-65 -1.5807773085414283 2316 66-67 -1.4104151279434234 2316 68-69 -1.4239296283777776 2316 70-71 -1.457675833940005 2316 72-73 -1.3577156425196364 2316 74-75 -1.4194294883365934 2316 76-77 -1.6391818288411564 2316 78-79 -1.1876039713918836 2316 80-81 -1.2573291919155558 2316 82-83 -1.2124881520195707 2316 84-85 -0.8880348114134691 2316 86-87 -0.2709621231397854 2316 88-89 -0.2015153156493419 2316 90-91 -0.6872852456957368 2316 92-93 -0.7549608969149126 2316 94-95 -0.5063586945629233 2316 96-97 -1.21662699404855 2316 98-99 -0.904119509955482 2316 100-101 0.02455864942758268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 160.0 8 346.0 9 234.0 10 200.0 11 214.0 12 204.0 13 213.0 14 199.0 15 184.0 16 269.0 17 479.0 18 534.0 19 717.0 20 796.0 21 749.0 22 606.0 23 490.0 24 449.0 25 429.0 26 420.0 27 408.0 28 401.0 29 455.0 30 532.0 31 616.0 32 721.0 33 1000.0 34 1416.0 35 3029.0 36 4024.0 37 8609.0 38 12980.0 39 3025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82177691079979 21.28701897499557 14.045043447419756 19.84616066678489 2 17.352367441035646 23.723177868416386 37.54655080688065 21.37790388366732 3 18.859726901932966 28.82824968966129 32.06907253059053 20.242950877815215 4 11.89705621564107 21.752527043802093 38.528551161553466 27.82186557900337 5 11.560117042028729 38.51746763610569 35.609150558609684 14.313264763255894 6 25.257137790388366 39.10932789501685 17.7624578826033 17.871076431991487 7 22.235768753325058 37.76822131583614 23.656676715729738 16.33933321510906 8 23.23993615889342 34.232576697996095 22.142667139563752 20.38482000354673 9 24.993349884731337 17.39448483773719 23.206685582550097 34.40547969498138 10-11 22.8675297038482 27.460542649405923 28.953493527221134 20.71843411952474 12-13 25.04765915942543 28.35165809540699 27.64785422947331 18.95282851569427 14-15 22.231335343145947 30.095096648341908 27.477167937577583 20.196400070934565 16-17 18.841993261216526 32.44480404327008 28.570003546728145 20.143199148785246 18-19 18.57266359283561 31.687799255187088 31.16133179641781 18.578205355559497 20-21 19.88051959567299 30.559496364603657 31.631273275403437 17.928710764319916 22-23 19.662174144351834 31.039413016492283 30.873160134775667 18.42525270438021 24-25 19.43163681503813 31.512679553112257 30.558388012058874 18.497295619790744 26-27 19.335210143642488 30.625997517290298 31.23780812200745 18.800984217059764 28-29 18.777708813619437 30.406543713424366 32.12116510019507 18.69458237276113 30-31 19.622273452739847 30.31455045220784 31.273275403440326 18.789900691611987 32-33 18.700124135485016 30.844342968611453 31.94826210320979 18.50727079269374 34-35 19.164523851746765 30.582771768043983 31.26440858308211 18.98829579712715 36-37 19.191124312821422 31.049388189395284 30.67698173435006 19.082505763433232 38-39 19.24875864514985 31.070446887746055 30.933011172193652 18.747783294910445 40-41 18.969453803865935 31.049388189395284 31.44507004788083 18.53608795885795 42-43 19.07142223798546 30.92636105692499 30.476369923745345 19.52584678134421 44-45 18.849751729029972 30.338934208192942 30.961828338357865 19.849485724419225 46-47 19.735325412307148 30.2081486079092 29.97096116332683 20.08556481645682 48-49 18.895194183365845 29.803599929065438 30.490778506827453 20.810427380741267 50-51 18.97499556658982 29.355825500975353 31.031654548678844 20.637524383755984 52-53 19.089155878701895 29.372450789147013 30.712449015782937 20.82594431636815 54-55 18.691257315126798 29.901134953005855 30.594963646036533 20.81264408583082 56-57 18.938419932612167 29.438951941833658 30.844342968611453 20.77828515694272 58-59 19.0348466040078 29.151888632736302 30.79557545664125 21.01768930661465 60-61 18.89186912573151 29.891159780102853 30.497428622096116 20.719542472069516 62-63 18.920686291895727 29.547570491221848 30.323417272566054 21.208325944316368 64-65 18.621431104805815 29.91000177336407 30.22255719099131 21.246009930838802 66-67 19.051471892179464 29.257182124490157 29.824658627416206 21.86668735591417 68-69 18.756650115268663 29.09536265295265 30.18598155701365 21.962005674765027 70-71 19.24875864514985 28.496852278772835 29.989803156588046 22.26458591948927 72-73 19.645548856180174 27.951542826742333 29.939927292073065 22.46298102500443 74-75 19.132381627948217 28.85374179819117 29.295974463557368 22.717902110303246 76-77 19.327451675829046 28.411509132824968 29.380209256960455 22.88082993438553 78-79 19.4526955133889 28.189838623869477 29.53870367086363 22.81876219187799 80-81 19.62116510019507 28.246364603653127 29.46333569781876 22.669134598333038 82-83 19.482621032097892 28.259664834190456 29.440060294378434 22.817653839333214 84-85 18.986079092037595 28.135529349175386 29.555328959035286 23.32306259975173 86-87 19.487054442277 28.139962759354496 29.66727256605781 22.705710232310693 88-89 20.062289413016494 27.23222202518177 29.494369569072532 23.211118992729208 90-91 19.98913814506118 26.64368682390495 30.609372229118637 22.757802801915233 92-93 20.0667228231956 27.243305550629543 30.06295442454336 22.627017201631496 94-95 20.221892179464444 26.612652952651178 30.52181237808122 22.643642489803156 96-97 20.542206064905123 26.934075190636637 30.286841638588403 22.236877105869834 98-99 20.285068274516757 26.48630076254655 29.881184607199856 23.347446355736835 100-101 20.76609327895017 26.282363894307505 30.385485015073595 22.566057811668735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 633.0 1 369.5 2 105.5 3 134.5 4 101.5 5 59.5 6 108.5 7 207.0 8 266.5 9 243.0 10 177.0 11 102.0 12 93.0 13 89.5 14 82.0 15 82.0 16 79.0 17 86.5 18 97.5 19 112.5 20 123.5 21 154.5 22 175.5 23 188.0 24 216.0 25 262.5 26 342.0 27 432.0 28 511.5 29 536.0 30 617.0 31 733.5 32 854.0 33 1034.5 34 1197.5 35 1318.0 36 1426.5 37 1496.0 38 1539.0 39 1545.5 40 1474.0 41 1536.5 42 1655.5 43 1684.0 44 1630.0 45 1574.5 46 1575.5 47 1681.0 48 1953.0 49 1993.5 50 1686.0 51 1305.0 52 985.0 53 766.5 54 736.0 55 770.5 56 723.0 57 690.5 58 589.5 59 482.5 60 432.0 61 345.0 62 255.0 63 190.0 64 146.5 65 125.0 66 96.5 67 63.0 68 50.5 69 45.5 70 39.0 71 31.5 72 26.0 73 21.5 74 19.5 75 18.0 76 17.0 77 16.5 78 14.0 79 10.0 80 8.0 81 6.0 82 3.5 83 4.5 84 5.5 85 4.5 86 3.5 87 2.5 88 1.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 45112.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.98146834545132 #Duplication Level Percentage of deduplicated Percentage of total 1 73.82954688088364 39.11597801028551 2 11.903267645705201 12.613051959567297 3 5.188067444876784 8.246142933144176 4 2.9664030793690643 6.286575633977656 5 1.7781682774779297 4.7104983153041315 6 1.0292456382578135 3.2718567121830113 7 0.8284172210367767 3.0723532541230716 8 0.543910296640308 2.305373293137081 9 0.35981758085435755 1.7157297393154816 >10 1.493661353081461 11.608884553998935 >50 0.03765532822894439 1.4297747827629013 >100 0.03765532822894439 4.444493704557546 >500 0.0041839253587716 1.179287107643199 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 532 1.179287107643199 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.7736300762546551 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 348 0.7714133711651002 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.6650115268664657 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 262 0.58077673346338 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 223 0.49432523497073944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.4012236212094343 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 125 0.2770881361943607 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.25270438020925695 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.22832062422415322 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 89 0.1972867529703848 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 89 0.1972867529703848 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.1795531122539457 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75 0.16625288171661642 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 68 0.15073594608973223 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 67 0.14851924100017733 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.1396524206419578 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 60 0.13300230537329313 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.11748536974640893 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 49 0.10861854938818939 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 46 0.10196843411952475 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.004433410179109771 0.0 16-17 0.0 0.0 0.0 0.006650115268664657 0.0 18-19 0.0 0.0 0.0 0.006650115268664657 0.0 20-21 0.0 0.0 0.0 0.009975172902996984 0.0 22-23 0.0 0.0 0.0 0.021058698350771413 0.0 24-25 0.0 0.0 0.0 0.05430927469409469 0.0 26-27 0.0 0.0 0.0 0.0931016137613052 0.0 28-29 0.0 0.0 0.0 0.20837027841815925 0.0 30-31 0.0 0.0 0.0 0.38570668558255006 0.0 32-33 0.0 0.0 0.0 0.5885352012768221 0.0 34-35 0.0 0.0 0.0 0.9010906189040611 0.0 36-37 0.0 0.0 0.0 1.2590884908671751 0.0 38-39 0.0 0.0 0.0 1.6359283560915054 0.0 40-41 0.0 0.0 0.0 2.134687001241355 0.0 42-43 0.0 0.0 0.0 2.46608441212981 0.0 44-45 0.0 0.0 0.0 2.744280900868948 0.0 46-47 0.0 0.0 0.0 2.9870101081752085 0.0 48-49 0.0 0.0 0.0 3.2474729561979077 0.0 50-51 0.0 0.0 0.0 3.5035023940414964 0.0 52-53 0.0 0.0 0.0 3.706330909735769 0.0 54-55 0.0 0.0 0.0 3.984527398474907 0.0 56-57 0.0 0.0 0.0 4.40348466040078 0.0 58-59 0.0 0.0 0.0 4.731557013654903 0.0 60-61 0.0 0.0 0.0 4.992019861677602 0.0 62-63 0.0 0.0 0.0 5.218123780812201 0.0 64-65 0.0 0.0 0.0 5.48302003901401 0.0 66-67 0.0 0.0 0.0 5.826609327895017 0.0 68-69 0.0 0.0 0.0 6.263300230537329 0.0 70-71 0.0 0.0 0.0 6.70109948572442 0.0 72-73 0.0 0.0 0.0 7.1045398120234085 0.0 74-75 0.0 0.0 0.0 7.542339067210499 0.0 76-77 0.0 0.0 0.0 8.015605603830465 0.0 78-79 0.0 0.0 0.0 8.460054974286221 0.0 80-81 0.0 0.0 0.0 8.955488561801737 0.0 82-83 0.0 0.0 0.0 9.432080156056038 0.0 84-85 0.0 0.0 0.0 9.975172902996984 0.0 86-87 0.0 0.0 0.0 10.533782585564817 0.0 88-89 0.0 0.0 0.0 11.140051427558078 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 25 0.004990134 57.0 1 AGCTATT 25 0.004990134 57.0 8 CGCAGAA 105 0.0 54.285713 9 TCTCTGA 25 0.0016079319 38.0 20-21 CAGAAAA 110 0.0 36.704544 10-11 TTATATA 55 0.002553543 34.545456 2 ATGTAAA 60 0.0039111814 31.666668 1 ACCAGTC 30 0.003931845 31.666668 54-55 ATAGTAT 60 0.0039111814 31.666668 6 CATGGGG 65 0.005785509 29.23077 4 GTGATGT 65 0.005785509 29.23077 9 AACACTG 85 6.311496E-4 27.941175 7 GTCATGC 35 0.008351351 27.142859 58-59 ACTAGAG 35 0.008351351 27.142859 78-79 CATAGGG 35 0.008351351 27.142859 18-19 AATCTCT 35 0.008351351 27.142859 18-19 GCCAAGA 35 0.008351351 27.142859 84-85 TGGTCCA 35 0.008351351 27.142859 10-11 GAGTACT 230 3.6379788E-12 26.847826 1 GGTATCA 630 0.0 26.388887 1 >>END_MODULE