FastQCFastQC Report
Fri 10 Feb 2017
SRR2031815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45112
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11052.4494591239581487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4891.083968788792339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.9243660223443874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.8999822663592836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.8755985103741799No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.7270792693740025No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2840.6295442454335876RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG2440.540876041851392RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT2080.4610746586274162RNA PCR Primer, Index 39 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.4499911331796418No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.3568895194183366No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.3214222379854584No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1360.30147189217946446No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT960.212803688597269No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.1839865224330555No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.17733640716439084No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT780.17290299698528105No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT750.16625288171661642RNA PCR Primer, Index 39 (96% over 28bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG730.16181947153750667No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA680.15073594608973223RNA PCR Primer, Index 39 (96% over 30bp)
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT670.14851924100017733No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.12856889519418335No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12413548501507359No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG550.12191877992551871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA558.015777E-751.8175749
GGTATCA4450.037.4005781
GGTACTC352.0385599E-433.92817750-51
CAGAAAA701.6694685E-830.5353610-11
GTATCAA6000.027.708012
GAGTACT2950.027.4029431
AAGGACG350.00835182727.14254258-59
AACGCAG6100.025.6964237
GTACTTT3150.025.6346233
AGTACTT3200.025.2340812
CAACGCA6300.024.8806636
TCAACGC6300.024.8806635
CTTGAAA2350.024.25503556-57
ATCAACG6600.023.7497234
AGCCCCC500.001642011623.74972278-79
ACTTTTT3400.023.7497225
TATCAAC6650.023.5711543
ACGCAGA6500.023.3843428
TTGAAAA2500.021.84974558-59
CTGCTTG2750.021.59065854-55
Produced by FastQC (version 0.11.5)