##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031815_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45112 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.714421883312646 34.0 31.0 34.0 31.0 34.0 2 32.82944671040965 34.0 31.0 34.0 31.0 34.0 3 32.82248625642845 34.0 33.0 34.0 31.0 34.0 4 36.25611810604717 37.0 37.0 37.0 35.0 37.0 5 36.28710764319915 37.0 37.0 37.0 35.0 37.0 6 36.39226813264763 37.0 37.0 37.0 35.0 37.0 7 36.350062067742506 37.0 37.0 37.0 35.0 37.0 8 36.40496985281078 37.0 37.0 37.0 35.0 37.0 9 38.15971360170243 39.0 39.0 39.0 37.0 39.0 10-11 38.188608352544776 39.0 39.0 39.0 37.0 39.0 12-13 38.2026955133889 39.0 39.0 39.0 37.0 39.0 14-15 39.69954779216173 41.0 40.0 41.0 37.0 41.0 16-17 39.62000133002306 41.0 40.0 41.0 37.0 41.0 18-19 39.653883667316904 41.0 40.0 41.0 37.0 41.0 20-21 39.65771856712183 41.0 40.0 41.0 37.0 41.0 22-23 39.53778373825146 41.0 39.5 41.0 35.5 41.0 24-25 39.40119037063309 41.0 39.0 41.0 35.0 41.0 26-27 39.17620588756871 41.0 39.0 41.0 35.0 41.0 28-29 39.05430927469409 41.0 38.5 41.0 35.0 41.0 30-31 38.85944981379677 40.5 38.0 41.0 35.0 41.0 32-33 38.28497960631317 40.0 37.0 41.0 35.0 41.0 34-35 38.23110258911154 40.0 37.0 41.0 35.0 41.0 36-37 38.06116997694627 40.0 37.0 41.0 34.5 41.0 38-39 37.73510374179819 40.0 36.0 41.0 34.0 41.0 40-41 37.59408804752616 40.0 36.5 41.0 33.0 41.0 42-43 37.33338136194361 40.0 36.0 41.0 33.0 41.0 44-45 36.96396745876929 40.0 36.0 41.0 32.0 41.0 46-47 36.60146967547438 40.0 36.0 41.0 30.5 41.0 48-49 36.3495965596737 40.0 35.5 41.0 27.5 41.0 50-51 36.185316545486785 40.0 36.0 41.0 26.0 41.0 52-53 36.26824348288704 40.0 36.0 41.0 26.0 41.0 54-55 36.13178311757404 40.0 36.0 41.0 24.0 41.0 56-57 35.86767378967902 40.0 35.0 41.0 22.5 41.0 58-59 35.60892888810072 40.0 35.0 41.0 21.0 41.0 60-61 35.211373913814505 39.0 35.0 41.0 19.0 41.0 62-63 34.81244458237276 39.0 35.0 41.0 16.5 41.0 64-65 34.44666607554531 38.5 35.0 41.0 15.0 41.0 66-67 33.928788348998054 37.0 35.0 40.5 11.5 41.0 68-69 33.26548368505054 37.0 34.0 39.5 10.0 41.0 70-71 32.68612564284447 36.0 34.0 39.0 9.5 41.0 72-73 32.151567210498314 36.0 33.5 39.0 8.0 41.0 74-75 31.78913592835609 35.0 33.0 37.5 8.0 40.0 76-77 30.49966749423657 34.5 31.0 36.0 8.0 39.0 78-79 31.094487054442276 35.0 32.5 36.5 8.0 39.0 80-81 30.971936513566234 35.0 33.0 36.0 8.0 38.0 82-83 30.717957527930487 35.0 33.0 36.0 8.0 37.0 84-85 30.50516492285866 35.0 33.0 35.5 8.0 37.0 86-87 30.306869569072532 35.0 32.0 35.0 7.0 36.0 88-89 30.153861500266004 35.0 32.0 35.0 7.0 36.0 90-91 29.95239625820181 35.0 32.0 35.0 7.0 36.0 92-93 29.84468655790034 35.0 32.0 35.0 7.0 36.0 94-95 29.77777531477212 35.0 32.0 35.0 7.0 35.0 96-97 29.69692099663061 35.0 31.5 35.0 7.0 35.0 98-99 29.649306171306968 35.0 31.0 35.0 7.0 35.0 100-101 28.683332594431636 34.0 28.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32317328675859613 1101 2 0.5443775433096221 1101 3 0.6167265776452595 1101 4 0.46126956073808145 1101 5 0.40900864172841267 1101 6 0.4289492108544266 1101 7 0.5024403530048431 1101 8 0.11558510099064989 1101 9 0.0577881488670684 1101 10-11 0.5365671084447143 1101 12-13 0.36051581397877897 1101 14-15 0.30431279286556645 1101 16-17 0.4023758189521942 1101 18-19 0.7075878282066554 1101 20-21 0.4631570155853879 1101 22-23 0.552794089602024 1101 24-25 0.3182603984432717 1101 26-27 0.46155549232558 1101 28-29 0.5652067985113263 1101 30-31 1.0772326256298896 1101 32-33 0.5891264882460163 1101 34-35 0.7078449749975704 1101 36-37 0.7485568407086163 1101 38-39 0.38354342583548373 1101 40-41 0.351178579805854 1101 42-43 0.6312411191826612 1101 44-45 1.2942595599950764 1101 46-47 1.1505324921022293 1101 48-49 1.1768561172776941 1101 50-51 1.5696269192188836 1101 52-53 1.5222333878094219 1101 54-55 0.9182065086622728 1101 56-57 0.7015232296575888 1101 58-59 0.7358530767206446 1101 60-61 0.41135815769272455 1101 62-63 0.6593513563903102 1101 64-65 0.8574685092503032 1101 66-67 0.9983103752424896 1101 68-69 0.6905418080691064 1101 70-71 0.6359762214318962 1101 72-73 0.7468130235313666 1101 74-75 1.3222415706578516 1101 76-77 0.7815322869264456 1101 78-79 1.1703634633707019 1101 80-81 1.2843464881219617 1101 82-83 1.4094061837820888 1101 84-85 1.3596892165292545 1101 86-87 1.5327790002771735 1101 88-89 1.4893575905791998 1101 90-91 2.1587490647746783 1101 92-93 1.7016447323080932 1101 94-95 1.3160720204930847 1101 96-97 1.7484750119591794 1101 98-99 1.767331337226043 1101 100-101 1.2477068671846112 1102 1 0.08934975734683093 1102 2 0.16202460213315106 1102 3 -0.19944989294297244 1102 4 -0.31961279220309535 1102 5 -0.37187371121276414 1102 6 -0.4284037303220458 1102 7 -0.3299125881716307 1102 8 -0.28882666371523413 1102 9 -0.25250596877999243 1102 10-11 -0.37593289155528 1102 12-13 0.1303687551552528 1102 14-15 0.21240102815968243 1102 16-17 -0.03585947516545929 1102 18-19 0.19361723997136693 1102 20-21 -0.022137102061670078 1102 22-23 -0.11558826333915562 1102 24-25 0.08002510432561394 1102 26-27 0.5365554923255829 1102 28-29 0.6630009161583814 1102 30-31 0.6787032138651767 1102 32-33 0.3957441353048452 1102 34-35 0.670344974997569 1102 36-37 0.9639980171792004 1102 38-39 0.6445728376001867 1102 40-41 0.7497079915705598 1102 42-43 1.2643293544767715 1102 44-45 0.6876419129362503 1102 46-47 0.7909736685728248 1102 48-49 0.5275914113953419 1102 50-51 1.0247739780424112 1102 52-53 1.237674564280006 1102 54-55 1.3086476851328612 1102 56-57 1.560346759069354 1102 58-59 1.909382488485349 1102 60-61 1.97826992239861 1102 62-63 2.288027826978542 1102 64-65 1.5559979210150061 1102 66-67 1.5813986105366027 1102 68-69 1.8567182786573326 1102 70-71 1.8175938684907251 1102 72-73 1.4799012588254854 1102 74-75 2.1104768647755066 1102 76-77 2.0263852281029138 1102 78-79 2.1755105221942337 1102 80-81 2.0336111940043153 1102 82-83 1.591759124958557 1102 84-85 1.7001303929998386 1102 86-87 1.9702790002771735 1102 88-89 2.4268575905792 1102 90-91 2.036690241245264 1102 92-93 1.8303212028963287 1102 94-95 2.2587190793166165 1102 96-97 2.329357364900357 1102 98-99 2.118066631343691 1102 100-101 2.1690303965963764 1103 1 -0.11800318382964292 1103 2 0.1914363668390351 1103 3 0.3814324599982015 1103 4 -0.06814220396779547 1103 5 0.08547922996370971 1103 6 -0.07105078914557339 1103 7 -0.1446184705245699 1103 8 -0.6785325460681761 1103 9 -0.05985890995646059 1103 10-11 0.2277435790329463 1103 12-13 -0.227719480138866 1103 14-15 -0.10745191301678858 1103 16-17 -0.06821241634192887 1103 18-19 -0.20417687767569248 1103 20-21 0.13962760382068495 1103 22-23 -0.21191179275091798 1103 24-25 -0.8287984250861484 1103 26-27 -0.597268037086188 1103 28-29 -0.46420496619455776 1103 30-31 -0.2315909037818784 1103 32-33 -0.13146174704809965 1103 34-35 0.10490379852697629 1103 36-37 0.05738037012037722 1103 38-39 0.9129551905413607 1103 40-41 0.6305903445117309 1103 42-43 0.9988881780061902 1103 44-45 1.4560242658774243 1103 46-47 0.9152383744551784 1103 48-49 0.9856796466894551 1103 50-51 0.3931563309835866 1103 52-53 0.4634098583976609 1103 54-55 0.7417359204269758 1103 56-57 1.1868173473046468 1103 58-59 0.39761778260300673 1103 60-61 0.5290052165162606 1103 62-63 1.2622925328608972 1103 64-65 0.6662920386620641 1103 66-67 0.4394868458307215 1103 68-69 0.20524769042204838 1103 70-71 0.5330350449613093 1103 72-73 0.17328361176665652 1103 74-75 0.7634180412460907 1103 76-77 0.8991793457499746 1103 78-79 0.47918699278246635 1103 80-81 0.24022884106313924 1103 82-83 0.1299944190762048 1103 84-85 0.24204215770572546 1103 86-87 1.1357201767477605 1103 88-89 0.5775928846968448 1103 90-91 1.2028667118335008 1103 92-93 1.7310564970139737 1103 94-95 0.9631308440224977 1103 96-97 1.1455338354885924 1103 98-99 0.8702725136966301 1103 100-101 0.4241774554199047 1104 1 -0.2221367291396561 1104 2 -0.5136510735425261 1104 3 -0.06769313618621453 1104 4 -0.7851533327436329 1104 5 -0.0536304679695192 1104 6 -0.26421454113285847 1104 7 -0.244777453036491 1104 8 -0.25166450155307274 1104 9 -0.39372218499620715 1104 10-11 -0.6999193780417698 1104 12-13 -0.6219961097096203 1104 14-15 -0.8332070799484299 1104 16-17 -0.41558920489518414 1104 18-19 -0.5161800573259328 1104 20-21 -1.1370019669265403 1104 22-23 -0.08552069577158505 1104 24-25 -0.23619111189059794 1104 26-27 -0.11276883199874277 1104 28-29 -0.28058016492269644 1104 30-31 -0.3206211104591432 1104 32-33 -1.1775666755059646 1104 34-35 -0.07593880878621917 1104 36-37 -0.2701236044424178 1104 38-39 -0.7929271623998133 1104 40-41 -0.8725893057267413 1104 42-43 -1.0468192941718684 1104 44-45 -1.7431013303069918 1104 46-47 -0.7330128179136608 1104 48-49 -0.417341021037096 1104 50-51 -0.055969265200829454 1104 52-53 -0.42887948977404733 1104 54-55 -0.37750096351578577 1104 56-57 -0.44911270039010276 1104 58-59 -0.7041310250281612 1104 60-61 0.10968884131752787 1104 62-63 0.3167440431947526 1104 64-65 -0.0014345114174290075 1104 66-67 0.14457428621228274 1104 68-69 0.7398263867654435 1104 70-71 0.22063440903126263 1104 72-73 0.7038877453119667 1104 74-75 0.7685849728836089 1104 76-77 0.45442576864345696 1104 78-79 1.1799024140861185 1104 80-81 1.5407057885989133 1104 82-83 1.5620293952288336 1104 84-85 1.0031709335403818 1104 86-87 0.8571033246014963 1104 88-89 0.30692515814676824 1104 90-91 0.092771322326346 1104 92-93 -0.08690852683340111 1104 94-95 1.0080434036409365 1104 96-97 1.3935465540895464 1104 98-99 0.9366477124247687 1104 100-101 -0.15292906286308394 1105 1 0.33752048905414256 1105 2 0.23519533384046554 1105 3 0.1511598631545894 1105 4 -0.025100597081141984 1105 5 0.2884921424457758 1105 6 0.03440114772673297 1105 7 0.010331314267396863 1105 8 -0.7065095905444991 1105 9 -0.15311572487755143 1105 10-11 -0.1036767939943104 1105 12-13 0.14530777954549734 1105 14-15 0.2749010281596824 1105 16-17 0.16779906141990608 1105 18-19 0.2689221180201429 1105 20-21 0.4351799711090578 1105 22-23 0.38349710251450375 1105 24-25 0.467829982374397 1105 26-27 0.4554579313499758 1105 28-29 0.42245213567058215 1105 30-31 0.28419101874322905 1105 32-33 0.6820246231097187 1105 34-35 0.5371132676804962 1105 36-37 -0.19728247062567306 1105 38-39 -0.8295125282534741 1105 40-41 -0.14846274013675753 1105 42-43 -0.43475601137687647 1105 44-45 -0.8047361358442373 1105 46-47 -0.4172580387442508 1105 48-49 0.3565548260294875 1105 50-51 0.6019081243838826 1105 52-53 0.8324916374507367 1105 54-55 -0.3142181685256702 1105 56-57 -0.2701410458086926 1105 58-59 -0.09305653590489271 1105 60-61 -0.3336203215038367 1105 62-63 -0.19215509985072998 1105 64-65 -0.0324167131313331 1105 66-67 -0.4213452919024192 1105 68-69 -0.6731597701231564 1105 70-71 -1.3564914973629385 1105 72-73 -1.8069889850769556 1105 74-75 -0.9004987449805952 1105 76-77 -0.8544074548239102 1105 78-79 -1.041257770488695 1105 80-81 -1.6148644157517822 1105 82-83 -1.3179969726024154 1105 84-85 -1.5007842411464978 1105 86-87 -1.4916112436252646 1105 88-89 -1.7606424094208002 1105 90-91 -1.1224561002181481 1105 92-93 -1.235227577591477 1105 94-95 -2.21871994507363 1105 96-97 -1.7468621472947667 1105 98-99 -1.0346772710953367 1105 100-101 -0.7910305790133805 1106 1 0.38062882711427193 1106 2 0.4876059974819853 1106 3 0.7104338279872593 1106 4 0.3367825566341196 1106 5 0.37754489343840447 1106 6 0.43031719991051176 1106 7 0.4340409002004648 1106 8 0.16756868512197798 1106 9 0.5201684498246593 1106 10-11 0.47261362007262164 1106 12-13 0.47542689469013766 1106 14-15 0.6353661444387555 1106 16-17 0.6048381992531446 1106 18-19 0.8043730539248557 1106 20-21 0.5999559211941445 1106 22-23 0.6724931320096843 1106 24-25 0.7125832438605073 1106 26-27 0.5952764225581362 1106 28-29 0.6493381254607087 1106 30-31 0.8409125161907625 1106 32-33 1.086732507397862 1106 34-35 1.3938914866254777 1106 36-37 1.467777086946647 1106 38-39 1.7187007445769282 1106 40-41 1.8200568287798546 1106 42-43 1.997178191686082 1106 44-45 1.6164209827036942 1106 46-47 1.9744039011309695 1106 48-49 2.247649550930227 1106 50-51 2.5593670012982273 1106 52-53 2.4972675875358235 1106 54-55 2.31765931303984 1106 56-57 2.4638351311623765 1106 58-59 2.0419406280202423 1106 60-61 2.6093745735614036 1106 62-63 2.879597594420403 1106 64-65 2.2798351303173305 1106 66-67 2.573549773327308 1106 68-69 2.561950836796875 1106 70-71 2.273117124304676 1106 72-73 2.508098933244092 1106 74-75 2.509314074077828 1106 76-77 2.402257321126175 1106 78-79 2.206615173357026 1106 80-81 2.934483287027575 1106 82-83 2.873154473795765 1106 84-85 3.090537369744027 1106 86-87 3.044406907253915 1106 88-89 2.78586921848618 1106 90-91 3.3372716365940995 1106 92-93 3.1777049238265604 1106 94-95 2.7767423351305673 1106 96-97 2.7741248067608204 1106 98-99 3.0997526778553173 1106 100-101 2.3181583035731173 1107 1 0.18934975734682524 1107 2 0.4870246021331539 1107 3 0.400550107057029 1107 4 0.10538720779690891 1107 5 0.10312628878723729 1107 6 -0.10340373032204297 1107 7 0.020087411828370705 1107 8 0.1611733362847687 1107 9 0.29749403122001183 1107 10-11 0.2115671084447115 1107 12-13 -0.20713124484475287 1107 14-15 0.22490102815968527 1107 16-17 0.06414052483454213 1107 18-19 0.4061172399713655 1107 20-21 0.26536289793832424 1107 22-23 0.34691173666085007 1107 24-25 -0.09497489567438322 1107 26-27 -9.445076744256653E-4 1107 28-29 0.4130009161583814 1107 30-31 0.4412032138651796 1107 32-33 0.18324413530483952 1107 34-35 0.44534497499756753 1107 36-37 0.9514980171791976 1107 38-39 0.6070728376001924 1107 40-41 0.11220799157055694 1107 42-43 0.07682935447677153 1107 44-45 -0.21235808706374826 1107 46-47 -0.6715263314271738 1107 48-49 0.7525914113953363 1107 50-51 1.4997739780424126 1107 52-53 1.7251745642800032 1107 54-55 1.4586476851328598 1107 56-57 1.2853467590693555 1107 58-59 0.5093824884853575 1107 60-61 1.4907699223986057 1107 62-63 1.1005278269785421 1107 64-65 1.6684979210150033 1107 66-67 1.318898610536607 1107 68-69 1.1192182786573355 1107 70-71 2.392593868490728 1107 72-73 1.254901258825484 1107 74-75 0.9104768647755037 1107 76-77 0.8138852281029152 1107 78-79 1.4255105221942337 1107 80-81 1.5086111940043168 1107 82-83 2.16675912495856 1107 84-85 1.61263039299984 1107 86-87 1.5827790002771707 1107 88-89 1.0643575905792027 1107 90-91 1.4241902412452667 1107 92-93 1.280321202896328 1107 94-95 1.7712190793166123 1107 96-97 1.266857364900357 1107 98-99 0.980566631343688 1107 100-101 0.5940303965963771 1108 1 0.2643497573468281 1108 2 -0.019793579685028817 1108 3 -0.05172262021569907 1108 4 -0.1764309740212724 1108 5 -0.5014191657582145 1108 6 -0.2034037303220444 1108 7 0.25645104819200526 1108 8 0.08617333628476587 1108 9 0.14067584940183053 1108 10-11 0.0024761993538078286 1108 12-13 -0.32531306302657015 1108 14-15 0.07035557361422917 1108 16-17 0.3027768884709019 1108 18-19 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-3.688065939343339 2316 58-59 -3.2541095750067086 2316 60-61 -3.182245950617265 2316 62-63 -2.8756626492119324 2316 64-65 -2.7991608091437215 2316 66-67 -3.229514087876094 2316 68-69 -3.8206626737236142 2316 70-71 -3.177048988652132 2316 72-73 -2.4730749316507072 2316 74-75 -1.3871421828435437 2316 76-77 -1.0861147718970834 2316 78-79 -1.8389736047898957 2316 80-81 -1.9665872186940945 2316 82-83 -1.9939551607557249 2316 84-85 -2.418321987952538 2316 86-87 -2.4275384600402887 2316 88-89 -2.0542932030715946 2316 90-91 -2.7740240444690194 2316 92-93 -2.888924828849703 2316 94-95 -2.744852349254817 2316 96-97 -2.3194521589091686 2316 98-99 -2.1682428924658375 2316 100-101 -1.876009285943308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 5.0 15 35.0 16 119.0 17 294.0 18 545.0 19 560.0 20 655.0 21 724.0 22 651.0 23 499.0 24 429.0 25 468.0 26 638.0 27 649.0 28 772.0 29 971.0 30 677.0 31 563.0 32 651.0 33 816.0 34 1144.0 35 2592.0 36 3295.0 37 8269.0 38 15340.0 39 3747.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99702473466851 21.022247879568365 14.8519028376038 21.128824548159333 2 15.986877105869834 23.28870367086363 39.80758999822663 20.9168292250399 3 18.081663415499204 27.82186557900337 34.886504699414786 19.209966306082638 4 10.713335697818763 23.19338535201277 37.77930484128392 28.313974108884555 5 10.27664479517645 38.65268664656854 37.40246497605958 13.668203582195423 6 23.971448838446534 40.111278595495655 17.720340485901755 18.196932080156056 7 20.659691434651535 36.438198262103214 24.177602411775137 18.724507891470118 8 22.670242950877814 34.780102855116155 22.94954779216173 19.6001064018443 9 23.03156588047526 15.935892888810072 23.44387302713247 37.588668203582195 10-11 21.660533782585564 27.03604362475616 30.60826387657386 20.69515871608441 12-13 23.635618017378967 26.887524383755984 29.276024117751376 20.200833481113673 14-15 21.421129632913637 28.34722468522788 27.70105515162263 22.53059053023586 16-17 18.97056215641071 31.95158716084412 28.934651533959922 20.143199148785246 18-19 18.633622982798368 28.980093988295796 32.111189927292074 20.275093101613763 20-21 18.61034757935804 27.76090618904061 32.81388544068097 20.814860790920374 22-23 18.274516758290478 29.90667671572974 32.16217414435184 19.656632381627947 24-25 19.2609505231424 30.504078737364782 31.987054442277003 18.24791629721582 26-27 18.599264053910268 29.518753325057634 33.33370278418159 18.548279836850508 28-29 17.25372406455045 27.64785422947331 34.07297393154815 21.02544777442809 30-31 17.638322397588226 27.615712005674766 34.59279127504877 20.15317432168824 32-33 16.991044511438197 28.616554353608798 35.20681858485547 19.185582550097536 34-35 17.39448483773719 29.652863982975703 34.74796063131761 18.2046905479695 36-37 18.183631849618724 29.25274871431105 32.363894307501326 20.199725128568897 38-39 18.091638588402198 28.639829757049124 33.40463734704735 19.863894307501333 40-41 17.66270615357333 28.306215641071113 34.36668735591417 19.66439084944139 42-43 18.818926437826274 28.277711891646256 33.736408676279886 19.166952994247584 44-45 17.494236566767157 29.003369391736122 33.890095761659865 19.61229827983685 46-47 19.77300939882958 27.565836141159778 31.538171661642135 21.122982798368504 48-49 18.830010086119948 26.954324285381777 32.172505902042715 22.04315972645556 50-51 17.827850682745165 27.602411775137437 33.2007004788083 21.369037063309097 52-53 17.77908317077496 28.06570313885441 34.284669267600634 19.870544422769996 54-55 17.1971980847668 27.854007802801917 32.75403440326299 22.194759709168295 56-57 18.639164745522255 28.517910977123606 31.170198616776023 21.672725660578116 58-59 17.55408760418514 29.103121120766097 33.04553112253946 20.29726015250931 60-61 18.996054264940593 30.702473842879947 30.24472424188686 20.056747650292607 62-63 17.093012945557724 32.42485369746409 30.02748714311048 20.454646213867708 64-65 16.954468877460542 32.616598687710585 29.38907607731867 21.039856357510196 66-67 17.083037772654727 31.8640273098067 29.12528817166164 21.927646745876928 68-69 16.913459833303776 31.08707217591772 29.201764497251286 22.797703493527223 70-71 17.52748714311048 30.271324702961515 29.41567653839333 22.78551161553467 72-73 18.217990778506827 29.08760418513921 29.40902642312467 23.285378613229295 74-75 17.356800851214754 29.75372406455045 29.116421351303423 23.77305373293137 76-77 17.682656499379323 29.281565880475263 29.385751019684342 23.650026600461075 78-79 18.08609682567831 28.804974286220965 28.99671927646746 24.11220961163327 80-81 17.929819116864692 29.003369391736122 28.87258379145238 24.1942276999468 82-83 17.47539457350594 28.91470118815393 29.130829934385527 24.4790743039546 84-85 17.55741266181947 28.11557900336939 29.269374002482714 25.057634332328426 86-87 17.720340485901755 28.244147898563575 29.315924809363363 24.719586806171307 88-89 17.846692676006384 27.6434208192942 28.973443873027133 25.536442631672283 90-91 18.046196134066324 26.957350594076964 29.70273984749069 25.29371342436602 92-93 17.4233020039014 27.294289767689307 29.60631317609505 25.676095052314242 94-95 17.88215995743926 26.307856002837383 30.315658804752616 25.494325234970738 96-97 17.8311757403795 26.744546905479694 30.215907075722647 25.20837027841816 98-99 17.678223089200213 26.809939705621566 29.755940769640006 25.75589643553822 100-101 17.377859549565525 26.692454335875155 30.323417272566054 25.606268841993263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 676.0 1 385.0 2 101.0 3 142.5 4 110.0 5 56.0 6 92.0 7 165.0 8 248.0 9 267.0 10 174.5 11 86.5 12 79.5 13 85.5 14 85.0 15 70.0 16 73.5 17 101.0 18 99.5 19 97.0 20 111.0 21 127.0 22 131.5 23 145.0 24 168.0 25 204.0 26 268.5 27 347.0 28 404.0 29 417.0 30 531.0 31 671.0 32 825.0 33 994.0 34 1162.0 35 1353.5 36 1494.5 37 1637.5 38 1721.5 39 1761.5 40 1781.0 41 1852.0 42 1850.5 43 1860.5 44 1853.0 45 1763.0 46 1786.0 47 1815.0 48 1982.0 49 1924.5 50 1676.5 51 1468.5 52 1070.5 53 750.5 54 669.0 55 656.0 56 597.0 57 520.5 58 416.0 59 349.5 60 311.0 61 236.0 62 160.0 63 99.5 64 70.0 65 61.0 66 41.0 67 17.5 68 19.0 69 21.5 70 18.0 71 16.5 72 9.5 73 8.5 74 10.0 75 10.5 76 8.0 77 4.0 78 5.0 79 4.5 80 2.5 81 2.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16403617662706155 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0011083525447774427 44-45 0.0 46-47 0.0 48-49 0.0011083525447774427 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 45112.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.36238694804044 #Duplication Level Percentage of deduplicated Percentage of total 1 69.34257709777671 32.148873913814505 2 13.6026775041836 12.613051959567297 3 6.306478603872819 8.771502039368682 4 3.418599091561081 6.339776556126973 5 2.01290939517093 4.666164213513034 6 1.5013148458044465 4.176272388721404 7 0.8893138895529524 2.886150026600461 8 0.5737508964857757 2.12803688597269 9 0.46378197465933546 1.9351835431814153 >10 1.77384652163519 12.56428444759709 >50 0.05259383217786278 1.7489803156588046 >100 0.05737508964857757 7.572264585919489 >500 0.0 0.0 >1k 0.004781257470714798 2.4494591239581487 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1105 2.4494591239581487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 489 1.083968788792339 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 417 0.9243660223443874 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 406 0.8999822663592836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.8755985103741799 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 328 0.7270792693740025 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 284 0.6295442454335876 RNA PCR Primer, Index 39 (95% over 23bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 244 0.540876041851392 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 208 0.4610746586274162 RNA PCR Primer, Index 39 (96% over 25bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203 0.4499911331796418 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 161 0.3568895194183366 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.3214222379854584 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 136 0.30147189217946446 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 96 0.212803688597269 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.1839865224330555 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.17733640716439084 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 78 0.17290299698528105 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 75 0.16625288171661642 RNA PCR Primer, Index 39 (96% over 28bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 73 0.16181947153750667 No Hit CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 68 0.15073594608973223 RNA PCR Primer, Index 39 (96% over 30bp) GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 67 0.14851924100017733 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.12856889519418335 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.12413548501507359 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 55 0.12191877992551871 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.004433410179109771 0.0 16-17 0.0 0.0 0.0 0.006650115268664657 0.0 18-19 0.0 0.0 0.0 0.006650115268664657 0.0 20-21 0.0 0.0 0.0 0.009975172902996984 0.0 22-23 0.0 0.0 0.0 0.023275403440326296 0.0 24-25 0.0 0.0 0.0 0.057634332328427025 0.0 26-27 0.0 0.0 0.0 0.09864337648519242 0.0 28-29 0.0 0.0 0.0 0.21945380386593366 0.0 30-31 0.0 0.0 0.0 0.41009044156765384 0.0 32-33 0.0 0.0 0.0 0.6217857776201454 0.0 34-35 0.0 0.0 0.0 0.9365579003369391 0.0 36-37 0.0 0.0 0.0 1.2978808299343854 0.0 38-39 0.0 0.0 0.0 1.6736123426139387 0.0 40-41 0.0 0.0 0.0 2.1745876928533425 0.0 42-43 0.0 0.0 0.0 2.5126352190104626 0.0 44-45 0.0 0.0 0.0 2.7930484128391555 0.0 46-47 0.0 0.0 0.0 3.037994325234971 0.0 48-49 0.0 0.0 0.0 3.3073239936158894 0.0 50-51 0.0 0.0 0.0 3.5633534314594786 0.0 52-53 0.0 0.0 0.0 3.7672902996985282 0.0 54-55 0.0 0.0 0.0 4.044378435892889 0.0 56-57 0.0 0.0 0.0 4.466660755453095 0.0 58-59 0.0 0.0 0.0 4.783649583259443 0.0 60-61 0.0 0.0011083525447774427 0.0 5.0407873736478095 0.0 62-63 0.0 0.0022167050895548854 0.0 5.262457882603298 0.0 64-65 0.0 0.0022167050895548854 0.0 5.524029083170775 0.0 66-67 0.0 0.0022167050895548854 0.0 5.865401666962228 0.0 68-69 0.0 0.0022167050895548854 0.0 6.3065259797836495 0.0 70-71 0.0 0.0022167050895548854 0.0 6.7531920553289595 0.0 72-73 0.0 0.0022167050895548854 0.0 7.1688242596205 0.0 74-75 0.0 0.0022167050895548854 0.0 7.607731867352367 0.0 76-77 0.0 0.0022167050895548854 0.0 8.082106756517113 0.0 78-79 0.0 0.0022167050895548854 0.0 8.530989537151978 0.0 80-81 0.0 0.0022167050895548854 0.0 9.045265117928711 0.0 82-83 0.0 0.0022167050895548854 0.0 9.516314949459124 0.0 84-85 0.0 0.0022167050895548854 0.0 10.067166164213514 0.0 86-87 0.0 0.0022167050895548854 0.0 10.635751019684342 0.0 88-89 0.0 0.0022167050895548854 0.0 11.246453271856712 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 55 8.015777E-7 51.817574 9 GGTATCA 445 0.0 37.400578 1 GGTACTC 35 2.0385599E-4 33.928177 50-51 CAGAAAA 70 1.6694685E-8 30.53536 10-11 GTATCAA 600 0.0 27.70801 2 GAGTACT 295 0.0 27.402943 1 AAGGACG 35 0.008351827 27.142542 58-59 AACGCAG 610 0.0 25.696423 7 GTACTTT 315 0.0 25.634623 3 AGTACTT 320 0.0 25.234081 2 CAACGCA 630 0.0 24.880663 6 TCAACGC 630 0.0 24.880663 5 CTTGAAA 235 0.0 24.255035 56-57 ATCAACG 660 0.0 23.749723 4 AGCCCCC 50 0.0016420116 23.749722 78-79 ACTTTTT 340 0.0 23.749722 5 TATCAAC 665 0.0 23.571154 3 ACGCAGA 650 0.0 23.384342 8 TTGAAAA 250 0.0 21.849745 58-59 CTGCTTG 275 0.0 21.590658 54-55 >>END_MODULE