FastQCFastQC Report
Fri 10 Feb 2017
SRR2031814_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031814_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146137
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59100.5156451628382994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55250.48205406508995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.2675072875232193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.055.4757461
GTATCAA45800.039.825331
ATCAACG55800.032.3476683
TCAACGC56000.032.2321434
TATCAAC57300.031.5008752
CAACGCA57200.031.47295
AACGCAG57850.031.0371656
ACGCAGA63600.028.3058197
CGCAGAG63450.028.1481488
GTACATG35000.025.7857151
TACATGG35200.024.9644892
GCAGAGT71600.024.8777929
GAGTACT49800.023.6069312-13
CAGAGTA67650.023.2058410-11
AGAGTAC65100.022.50960210-11
ACATGGG36700.021.614443
GTACTTT55050.021.18301614-15
AGTACTT52850.021.0312212-13
CATGGGG19400.020.077324
ATCGCGT600.004764918719.79166840-41
Produced by FastQC (version 0.11.5)