##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031814_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1146137 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.605905751232186 34.0 31.0 34.0 31.0 34.0 2 32.731563504188415 34.0 31.0 34.0 31.0 34.0 3 32.7963821078981 34.0 33.0 34.0 31.0 34.0 4 35.99135618167811 37.0 37.0 37.0 35.0 37.0 5 36.0219336780856 37.0 37.0 37.0 35.0 37.0 6 36.1010394045389 37.0 37.0 37.0 35.0 37.0 7 36.12369899933429 37.0 37.0 37.0 35.0 37.0 8 36.12228119326049 37.0 37.0 37.0 35.0 37.0 9 37.95844475834913 39.0 39.0 39.0 37.0 39.0 10-11 37.99768701298362 39.0 39.0 39.0 37.0 39.0 12-13 37.96698693088173 39.0 39.0 39.0 37.0 39.0 14-15 39.53351388184833 41.0 40.0 41.0 37.0 41.0 16-17 39.468851454930785 41.0 40.0 41.0 37.0 41.0 18-19 39.48872996858142 41.0 40.0 41.0 37.0 41.0 20-21 39.43849295503068 41.0 40.0 41.0 37.0 41.0 22-23 39.347229868680614 41.0 40.0 41.0 36.5 41.0 24-25 39.304419105220404 41.0 40.0 41.0 36.0 41.0 26-27 39.18257415998262 41.0 39.5 41.0 36.0 41.0 28-29 39.05338716052269 41.0 39.0 41.0 35.5 41.0 30-31 38.8708653503028 41.0 39.0 41.0 35.0 41.0 32-33 38.70631695861839 41.0 38.5 41.0 35.0 41.0 34-35 38.50364616097377 40.0 38.0 41.0 35.0 41.0 36-37 38.37127498719612 40.0 38.0 41.0 35.0 41.0 38-39 38.1672361157523 40.0 38.0 41.0 34.0 41.0 40-41 38.05981527513727 40.0 38.0 41.0 34.0 41.0 42-43 37.86349930243941 40.0 38.0 41.0 33.0 41.0 44-45 37.96935488514899 40.0 38.0 41.0 33.0 41.0 46-47 37.775296932216655 40.0 38.0 41.0 33.0 41.0 48-49 37.55442106833651 40.0 38.0 41.0 32.5 41.0 50-51 36.47150864163709 39.0 36.5 40.0 30.5 40.5 52-53 36.4678258358294 39.5 36.5 40.0 30.5 40.5 54-55 37.214069958477914 40.0 37.0 41.0 31.5 41.0 56-57 37.186699321285325 40.0 37.0 41.0 31.0 41.0 58-59 37.04594564175138 40.0 36.5 41.0 31.0 41.0 60-61 36.83136570933492 40.0 36.0 41.0 31.0 41.0 62-63 36.54449991580414 39.0 35.5 41.0 30.5 41.0 64-65 36.21027460068038 39.0 35.0 41.0 30.0 41.0 66-67 35.920823601367026 39.0 35.0 41.0 29.0 41.0 68-69 35.58639848464887 37.5 35.0 40.5 29.0 41.0 70-71 35.22517945062414 37.0 35.0 40.0 29.0 41.0 72-73 34.82128707126635 36.5 35.0 39.0 28.0 41.0 74-75 34.46917035223538 36.0 35.0 39.0 28.0 41.0 76-77 34.10509869239017 35.5 35.0 38.0 27.0 40.5 78-79 33.74188862238982 35.0 34.5 37.0 26.5 39.0 80-81 33.39011610304877 35.0 34.0 37.0 26.0 39.0 82-83 33.10976567373709 35.0 34.0 36.0 26.0 38.5 84-85 32.811398201087655 35.0 34.0 36.0 25.0 37.0 86-87 32.556271196200804 35.0 34.0 36.0 25.0 37.0 88-89 32.35886242220607 35.0 34.0 35.0 24.5 36.5 90-91 32.18123531480094 35.0 34.0 35.0 24.0 36.0 92-93 32.04427917430464 35.0 34.0 35.0 23.5 36.0 94-95 31.878493583227833 35.0 34.0 35.0 23.0 36.0 96-97 31.781538332677506 35.0 34.0 35.0 21.5 36.0 98-99 31.6573393931092 35.0 33.0 35.0 20.0 35.0 100-101 30.176821357307198 34.0 30.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19138204995701358 1101 2 0.14894886091440895 1101 3 0.30930221288736703 1101 4 0.21041665518299624 1101 5 0.12467637918130947 1101 6 0.16465221006051678 1101 7 0.10967078100243555 1101 8 0.195858365323204 1101 9 0.30375693711654606 1101 10-11 0.353266484201157 1101 12-13 0.41554787930762416 1101 14-15 0.5094235824784974 1101 16-17 0.534518200202136 1101 18-19 0.5743826146515048 1101 20-21 0.47592497189847194 1101 22-23 0.4851121298334178 1101 24-25 0.42722958266017486 1101 26-27 0.3829578979151407 1101 28-29 0.5892871099478398 1101 30-31 0.5130606013194665 1101 32-33 0.32832705538930185 1101 34-35 0.5307541496869703 1101 36-37 0.5417186145251875 1101 38-39 0.5006302617008771 1101 40-41 0.5310745192153377 1101 42-43 0.3029013992448384 1101 44-45 0.49679647861453446 1101 46-47 0.5800480113546413 1101 48-49 0.5933666370413775 1101 50-51 0.6630575301596906 1101 52-53 0.7322378704920425 1101 54-55 0.7334413685885153 1101 56-57 0.7332432675205638 1101 58-59 0.571906897921238 1101 60-61 0.714624408324056 1101 62-63 0.7421163896009944 1101 64-65 0.6941849394317074 1101 66-67 0.6028826096209059 1101 68-69 0.737653696528298 1101 70-71 0.6364504659845451 1101 72-73 0.5752904107177415 1101 74-75 0.5964689549717903 1101 76-77 0.655413240283842 1101 78-79 0.6799998802480545 1101 80-81 0.5955054966138889 1101 82-83 0.36103448017014017 1101 84-85 0.41684425024444494 1101 86-87 0.41363098164882217 1101 88-89 0.4095784062741856 1101 90-91 0.33977421423627874 1101 92-93 0.3899817743581693 1101 94-95 0.49878650368599153 1101 96-97 0.32617152205707356 1101 98-99 0.38605902177149076 1101 100-101 0.4961404948791568 1102 1 0.1527552863749051 1102 2 0.19298598969037073 1102 3 0.22785941794136733 1102 4 0.27269609880745804 1102 5 0.16467716698608825 1102 6 0.22290342246890305 1102 7 0.18634040579882338 1102 8 0.18320946442673147 1102 9 0.24593090758313707 1102 10-11 0.34205880264111244 1102 12-13 0.2988867349980069 1102 14-15 0.3870983534937693 1102 16-17 0.3707949884610642 1102 18-19 0.46071918339094253 1102 20-21 0.3590402300551503 1102 22-23 0.3782278745753942 1102 24-25 0.3780908386527102 1102 26-27 0.4364677999261275 1102 28-29 0.3796980196538229 1102 30-31 0.3144845584628868 1102 32-33 0.40287134507901357 1102 34-35 0.4066980672547942 1102 36-37 0.5438317849991918 1102 38-39 0.5313633372212365 1102 40-41 0.3630792043367208 1102 42-43 0.34666221707887956 1102 44-45 0.39276280227717564 1102 46-47 0.5225481098302396 1102 48-49 0.46585753673760877 1102 50-51 0.44862517826119586 1102 52-53 0.481279760852793 1102 54-55 0.6597375970889772 1102 56-57 0.6144220320109568 1102 58-59 0.5323370856736602 1102 60-61 0.5215745843057391 1102 62-63 0.5670169292472735 1102 64-65 0.6337591541177758 1102 66-67 0.46575070561260645 1102 68-69 0.5786880146948477 1102 70-71 0.5764591318371473 1102 72-73 0.5161256923231932 1102 74-75 0.34407889428765515 1102 76-77 0.3735106222231863 1102 78-79 0.2780728865806097 1102 80-81 0.4708655118138907 1102 82-83 0.42603590516997514 1102 84-85 0.4587890622023991 1102 86-87 0.49827365425337433 1102 88-89 0.6005028951858336 1102 90-91 0.5395516593853742 1102 92-93 0.4721330143397253 1102 94-95 0.5038904359903924 1102 96-97 0.4503921166643181 1102 98-99 0.4868974545960718 1102 100-101 0.4141578219502122 1103 1 0.16203446959310241 1103 2 0.17649927970303736 1103 3 0.17382326705553908 1103 4 0.14854522153199667 1103 5 0.09201961318606067 1103 6 0.03200759933002928 1103 7 0.08469568793514526 1103 8 0.13769712744012708 1103 9 0.2032488016091989 1103 10-11 0.23540642424275404 1103 12-13 0.17465893185131165 1103 14-15 0.2239407174257977 1103 16-17 0.28907727649289683 1103 18-19 0.29288883485263995 1103 20-21 0.31901527188359324 1103 22-23 0.36294206291129427 1103 24-25 0.31812017732526954 1103 26-27 0.3287272409741959 1103 28-29 0.37958209663953113 1103 30-31 0.3344719297990295 1103 32-33 0.33383838875368355 1103 34-35 0.3760687495231707 1103 36-37 0.33260830038543787 1103 38-39 0.4031444702965814 1103 40-41 0.4143027530553667 1103 42-43 0.3961089918548879 1103 44-45 0.511754838312676 1103 46-47 0.541549334233693 1103 48-49 0.5472680794282709 1103 50-51 0.3154953423483491 1103 52-53 0.381104647654233 1103 54-55 0.4339078126737306 1103 56-57 0.3805230442272105 1103 58-59 0.517102557923721 1103 60-61 0.4946425162605408 1103 62-63 0.5029553073507529 1103 64-65 0.5426831962304206 1103 66-67 0.5177616760965691 1103 68-69 0.4223495365877312 1103 70-71 0.3990274729948382 1103 72-73 0.32683623095386594 1103 74-75 0.3611708613035489 1103 76-77 0.4550547167736312 1103 78-79 0.39368659477086965 1103 80-81 0.2675899654799423 1103 82-83 0.4182113688086062 1103 84-85 0.3469815820161486 1103 86-87 0.40215050448083645 1103 88-89 0.3893200103374497 1103 90-91 0.25005984023810157 1103 92-93 0.3325964286090439 1103 94-95 0.3462987072310142 1103 96-97 0.2688577446892708 1103 98-99 0.23676283432140366 1103 100-101 0.1525179760583768 1104 1 0.20071222592773097 1104 2 0.1264092314950176 1104 3 0.1574798102982129 1104 4 0.16574618725508117 1104 5 0.19436887522048352 1104 6 0.16678300620728947 1104 7 0.1339790135854031 1104 8 0.23509276137170332 1104 9 0.17602142695395884 1104 10-11 0.22411768007342658 1104 12-13 0.17454710408755858 1104 14-15 0.16459679968414775 1104 16-17 0.22216911394514227 1104 18-19 0.2385254034103852 1104 20-21 0.2814792330671523 1104 22-23 0.29975351641829917 1104 24-25 0.20562095865462027 1104 26-27 0.2752520833410017 1104 28-29 0.2941368951737573 1104 30-31 0.3582013906036394 1104 32-33 0.2521547997954272 1104 34-35 0.24088167974264252 1104 36-37 0.3835324176786017 1104 38-39 0.4859492668774834 1104 40-41 0.3383497265761193 1104 42-43 0.315442596049067 1104 44-45 0.4021274933479546 1104 46-47 0.3999475504587764 1104 48-49 0.3899449723939554 1104 50-51 0.33976587373044254 1104 52-53 0.3578166379344978 1104 54-55 0.32846936970686613 1104 56-57 0.3601161864994751 1104 58-59 0.27429811294102535 1104 60-61 0.25175554336485106 1104 62-63 0.4947269325939345 1104 64-65 0.5258689885374537 1104 66-67 0.34418203519554424 1104 68-69 0.28731384936949667 1104 70-71 0.44791525065975435 1104 72-73 0.35726559096699617 1104 74-75 0.3197667538551414 1104 76-77 0.36663899877101613 1104 78-79 0.3734130513641176 1104 80-81 0.2684489394382368 1104 82-83 0.24945838999044412 1104 84-85 0.3325470105362385 1104 86-87 0.32859664744848516 1104 88-89 0.2934362487054045 1104 90-91 0.37110973202398156 1104 92-93 0.29698978496555384 1104 94-95 0.1671818828660463 1104 96-97 -0.036742140455316985 1104 98-99 0.05176601823852067 1104 100-101 0.1860545852452482 1105 1 -0.003805844148459414 1105 2 -0.07861670360112072 1105 3 0.14568228037734343 1105 4 0.12103569786789592 1105 5 -0.02877305514414985 1105 6 0.08841566826490066 1105 7 0.1277386106730347 1105 8 0.07679591840025779 1105 9 0.1717840142920508 1105 10-11 0.10847644767880382 1105 12-13 0.09241210566717228 1105 14-15 0.21437124516534567 1105 16-17 0.22711459881388407 1105 18-19 0.21878157677144117 1105 20-21 0.17746608918981366 1105 22-23 0.3583366189317232 1105 24-25 0.17301519783057273 1105 26-27 0.2604248125506743 1105 28-29 0.31920595746941416 1105 30-31 0.3096084430395578 1105 32-33 0.17856350531820198 1105 34-35 0.3383169154728307 1105 36-37 0.45933287907350717 1105 38-39 0.4882116999274686 1105 40-41 0.32248743973248395 1105 42-43 0.36014022809397517 1105 44-45 0.36242861230407897 1105 46-47 0.45860504188108564 1105 48-49 0.5052216072968676 1105 50-51 0.5106789838113315 1105 52-53 0.4226171838205133 1105 54-55 0.47826291752515715 1105 56-57 0.39097518553700894 1105 58-59 0.47503933872330606 1105 60-61 0.35887649054689064 1105 62-63 0.39710939811789103 1105 64-65 0.4718130480779692 1105 66-67 0.36010723117817633 1105 68-69 0.27904979262086727 1105 70-71 0.30654326203299576 1105 72-73 0.4367832018523856 1105 74-75 0.23702286843977305 1105 76-77 0.24715709456189217 1105 78-79 0.27775993511169617 1105 80-81 0.3214244039556391 1105 82-83 0.30982039764607094 1105 84-85 0.4786913691018029 1105 86-87 0.4324513705013331 1105 88-89 0.500286089641719 1105 90-91 0.5439073611300671 1105 92-93 0.4799336938914891 1105 94-95 0.4554462518644158 1105 96-97 0.5122640788248205 1105 98-99 0.4306741910999783 1105 100-101 0.4295120347893615 1106 1 0.0806290603720683 1106 2 0.03399426454070209 1106 3 0.0500386629123426 1106 4 0.1309895300977928 1106 5 0.05644940139024612 1106 6 0.007270901314818445 1106 7 0.12000929893788026 1106 8 0.0656571702132851 1106 9 0.1075805854807399 1106 10-11 0.030711965198243263 1106 12-13 0.09894977406471384 1106 14-15 0.0950212357021556 1106 16-17 0.1285625105004229 1106 18-19 -0.06334413195809674 1106 20-21 0.09019223008628074 1106 22-23 0.10759279489129625 1106 24-25 0.06374818630342816 1106 26-27 0.04061796910040982 1106 28-29 0.07945646718061283 1106 30-31 0.100067582275706 1106 32-33 0.10853192250908705 1106 34-35 0.08468614820162657 1106 36-37 -0.011302104919813871 1106 38-39 0.03940616876489145 1106 40-41 0.1718407590668889 1106 42-43 0.29131284639944255 1106 44-45 0.25914089659062256 1106 46-47 0.29146372114380625 1106 48-49 0.23629367368803855 1106 50-51 0.252112561692428 1106 52-53 0.39533406784136815 1106 54-55 0.33474591474361404 1106 56-57 0.21933100476601197 1106 58-59 0.344635346067804 1106 60-61 0.38715301638406174 1106 62-63 0.37301371503313163 1106 64-65 0.4173055685762961 1106 66-67 0.3641512838598402 1106 68-69 0.38378882856032703 1106 70-71 0.33702547169944097 1106 72-73 0.32045602870665846 1106 74-75 0.4837692829701936 1106 76-77 0.42618569669294715 1106 78-79 0.4735051808718751 1106 80-81 0.5743371354784443 1106 82-83 0.5347019284749663 1106 84-85 0.5420220324125467 1106 86-87 0.6245346787938573 1106 88-89 0.6005787755439087 1106 90-91 0.4745664560552001 1106 92-93 0.5005759883049805 1106 94-95 0.5200174441446137 1106 96-97 0.4434860312541673 1106 98-99 0.41889454779466107 1106 100-101 0.2181238353323991 1107 1 0.2161188743608733 1107 2 0.20787328831830365 1107 3 0.21819132599401314 1107 4 0.0813344905847373 1107 5 0.2391787839167847 1107 6 0.19335768882148585 1107 7 0.13336499282095815 1107 8 0.10851433680270617 1107 9 0.18570628632449626 1107 10-11 0.13426739228308548 1107 12-13 0.13028403105820274 1107 14-15 0.1737611871601672 1107 16-17 0.33038474579121413 1107 18-19 0.2733366387997549 1107 20-21 0.3380899064829421 1107 22-23 0.2553169527574397 1107 24-25 0.31959664955554956 1107 26-27 0.36858834220263503 1107 28-29 0.3492507866706731 1107 30-31 0.30815359563554523 1107 32-33 0.30802133447314617 1107 34-35 0.3326913911358673 1107 36-37 0.22251100009004432 1107 38-39 0.33468669731113465 1107 40-41 0.30711460935088297 1107 42-43 0.2722536341353603 1107 44-45 0.3055527426982181 1107 46-47 0.3767805980428278 1107 48-49 0.38492820163290986 1107 50-51 0.30071602408898457 1107 52-53 0.3766565298940563 1107 54-55 0.31267151690856565 1107 56-57 0.41080691733726127 1107 58-59 0.49288695375146574 1107 60-61 0.5221951210002089 1107 62-63 0.38444982383231974 1107 64-65 0.4209615849097972 1107 66-67 0.3758789436605241 1107 68-69 0.39064027709534344 1107 70-71 0.3179911783243696 1107 72-73 0.43809436591903506 1107 74-75 0.42378204144137044 1107 76-77 0.31152765860692 1107 78-79 0.39642607336680413 1107 80-81 0.42515605323448113 1107 82-83 0.31457095479429853 1107 84-85 0.3850866072215453 1107 86-87 0.40257424334458136 1107 88-89 0.44971899080988464 1107 90-91 0.5632548586381247 1107 92-93 0.5596333399586868 1107 94-95 0.38806777365539347 1107 96-97 0.3827080303138928 1107 98-99 0.40072286329438 1107 100-101 0.5083491519268826 1108 1 0.12584154710729933 1108 2 0.16890165744128183 1108 3 0.11851477662840182 1108 4 0.18636481215089162 1108 5 0.23389452116489196 1108 6 0.24100873285340185 1108 7 0.25647858361728737 1108 8 0.28832446788207733 1108 9 0.28103617816779547 1108 10-11 0.19104734390450773 1108 12-13 0.26720605999266667 1108 14-15 0.286697822346774 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88-89 -0.29126772645766863 2316 90-91 -0.2517787794505395 2316 92-93 -0.6216242070483915 2316 94-95 -0.668369463996271 2316 96-97 -0.5124963857633915 2316 98-99 -0.5016404280632223 2316 100-101 -0.32561669247539626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 19.0 7 441.0 8 818.0 9 661.0 10 745.0 11 932.0 12 1132.0 13 1299.0 14 1426.0 15 1593.0 16 1960.0 17 2844.0 18 3871.0 19 5282.0 20 7152.0 21 9695.0 22 10938.0 23 10814.0 24 9324.0 25 7896.0 26 7448.0 27 7617.0 28 8316.0 29 9530.0 30 11106.0 31 13394.0 32 17044.0 33 22694.0 34 33635.0 35 56114.0 36 108411.0 37 249686.0 38 384352.0 39 137759.0 40 189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58039222187225 19.330324385304724 13.82408909231619 23.265194300506835 2 15.664706749716656 21.37109263552263 39.51255390934941 23.451646705411306 3 17.230924400835153 26.47397300671735 31.90613338545043 24.388969206997068 4 12.266945400069975 18.3733707226972 38.80600661177503 30.553677265457797 5 12.822376382579046 38.0817476444788 35.54269690272629 13.553179070215865 6 29.302256187523827 38.46686739892352 18.295718574655563 13.935157838897094 7 25.331264936041677 34.121837092773376 23.32565827645386 17.221239694731082 8 25.242095840200605 34.58609223853693 21.419428916438434 18.75238300482403 9 26.285950108931132 15.03040212470237 21.14825714552449 37.535390620842016 10-11 23.610179236862606 26.414905024442977 29.912828920102918 20.062086818591496 12-13 24.867053415080395 25.141322546955557 29.071480983512444 20.920143054451607 14-15 22.36216089350575 26.24184543383557 27.224057856957764 24.171935815700916 16-17 21.190529578924682 29.02406082344432 27.970041975784742 21.815367621846253 18-19 21.075796348953048 27.81853303749901 30.36857722942371 20.737093384124236 20-21 22.044048835348654 27.096760683932203 30.35911064733099 20.500079833388156 22-23 21.78061204012794 27.198434039998816 29.95923687806138 21.061717041811864 24-25 21.511253037382158 27.400873370065483 30.0581955877798 21.029678004772563 26-27 21.426845132824436 27.531045590535864 30.034280369624224 21.00782890701548 28-29 21.412405323272875 27.38311388603631 30.117996365181476 21.08648442550934 30-31 21.53926624827573 27.36064711286696 30.10551967173209 20.994566967125223 32-33 21.374408120495193 27.59281831055101 29.93834942943121 21.094424139522587 34-35 21.60657931817924 27.66091662689539 29.82701893403668 20.905485120888688 36-37 21.594451623148018 27.52930059844504 29.70281912197233 21.173428656434616 38-39 21.48761448238736 27.721555102051497 29.66770115614451 21.123129259416633 40-41 21.55745779082256 27.587365210267183 29.77693766103005 21.0782393378802 42-43 21.47574853616976 27.8837957416958 29.475490277340317 21.16496544479412 44-45 21.621760749369404 27.89365494700895 29.228573896488815 21.25601040713283 46-47 21.69539941560215 27.86905055852834 28.970794939871936 21.464755085997574 48-49 21.724497158716627 28.001320959012755 28.81871887915668 21.45546300311394 50-51 21.693828922720407 28.135772599610693 28.534372417957016 21.636026059711885 52-53 21.709184853119652 28.306476450895484 28.39516567391158 21.589173022073275 54-55 21.71948030645551 28.22502894505631 28.396648917188784 21.658841831299398 56-57 21.6822683501187 28.28706341388508 28.28863390676682 21.742034329229405 58-59 21.591048888570914 28.124561025427152 28.43246487985293 21.851925206149005 60-61 21.609720303942723 28.136208847633398 28.5249058358643 21.72916501255958 62-63 21.498215309339113 28.09681565118306 28.649192897533194 21.755776141944636 64-65 21.607800812642814 27.951588684424287 28.677461769404527 21.763148733528364 66-67 21.485913115098807 28.152960771705303 28.4120484723903 21.94907764080559 68-69 21.467547073342892 28.138302838142387 28.42264929934205 21.971500789172673 70-71 21.541054865168825 27.949625568322112 28.431330634993895 22.07798893151517 72-73 21.47269480001082 28.090969927678806 28.309312063043073 22.127023209267303 74-75 21.529188918951224 28.072385761911534 28.290727897275808 22.107697421861435 76-77 21.497517312502783 28.03696242246782 28.31629203140637 22.14922823362303 78-79 21.4869601103533 28.03255631743849 28.287717785919135 22.192765786289073 80-81 21.46135235142047 28.075483122872747 28.14968891153501 22.31347561417178 82-83 21.373884622867948 28.04302626998343 28.261193906138622 22.32189520101 84-85 21.464929585206654 27.87472178282352 28.23436465274221 22.425983979227617 86-87 21.468724943004197 27.813908808458326 28.209367641041165 22.50799860749631 88-89 21.471473305547242 27.71034352786796 28.217699978274847 22.60048318830995 90-91 21.526396931605905 27.591160568064726 28.255828055459336 22.62661444487003 92-93 21.537390381778096 27.467746002441245 28.38456484695983 22.61029876882083 94-95 21.44953003000514 27.34721067376762 28.491140238906866 22.712119057320372 96-97 21.60653569337697 27.27252501228038 28.388578328768727 22.73236096557392 98-99 21.57128685314234 27.296387779122387 28.252512570486775 22.879812797248498 100-101 21.827931564900183 27.043320301150736 28.408034990581406 22.720713143367675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1055.0 1 656.0 2 344.0 3 437.5 4 359.0 5 344.5 6 534.5 7 1005.0 8 1705.0 9 2132.0 10 1864.5 11 1506.0 12 1485.5 13 1543.0 14 1644.0 15 1749.0 16 1903.0 17 2039.5 18 2214.0 19 2403.5 20 2632.0 21 3048.5 22 3600.0 23 4472.0 24 5572.0 25 6852.5 26 8652.0 27 10827.0 28 12902.0 29 15014.0 30 17248.0 31 19512.5 32 22087.0 33 24465.5 34 26411.0 35 28429.0 36 30840.0 37 32790.0 38 34149.0 39 35085.0 40 35753.0 41 36957.5 42 38113.0 43 38927.0 44 39438.0 45 39862.5 46 40032.5 47 39962.5 48 40047.0 49 39751.5 50 39133.5 51 38412.0 52 37809.5 53 37057.0 54 35202.0 55 32064.5 56 28784.0 57 25598.5 58 22348.5 59 19311.5 60 16137.5 61 13032.5 62 10492.5 63 8118.5 64 5806.0 65 4213.5 66 3079.5 67 2259.5 68 1773.5 69 1381.5 70 1050.5 71 783.5 72 588.0 73 445.5 74 346.5 75 297.0 76 227.5 77 132.0 78 85.5 79 72.5 80 53.5 81 37.0 82 32.0 83 31.5 84 23.5 85 17.5 86 15.5 87 12.0 88 6.0 89 6.5 90 7.0 91 4.0 92 3.5 93 4.5 94 2.5 95 0.5 96 1.0 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6174881362350225E-4 24-25 1.7449920908233483E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1146137.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.957279538253225 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2220653808384 41.40101938411585 2 16.674550302034167 19.66172246663386 3 6.206558006576836 10.97765326092403 4 2.8395375390516358 6.696456337969238 5 1.4218347818588355 4.191375534563134 6 0.8439059724607579 2.9852640193422206 7 0.4952796971202412 2.0440240486917625 8 0.3133037159080429 1.477722780733117 9 0.21846038227282893 1.1591846843123552 >10 0.7436473150969046 6.688466458772953 >50 0.01401228214586181 0.5457192333801456 >100 0.005505464110771111 0.563289307447864 >500 8.927736831668058E-4 0.3359899901982646 >1k 1.487956138611343E-4 0.2689674438506212 >5k 2.975912277222686E-4 1.0031450490645315 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5910 0.5156451628382994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5525 0.48205406508995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3066 0.2675072875232193 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.724960454116742E-5 0.0 3 0.0 0.0 0.0 8.724960454116742E-5 0.0 4 0.0 0.0 0.0 8.724960454116742E-5 0.0 5 0.0 0.0 0.0 8.724960454116742E-5 0.0 6 0.0 0.0 0.0 8.724960454116742E-5 0.0 7 0.0 0.0 0.0 8.724960454116742E-5 0.0 8 0.0 0.0 0.0 8.724960454116742E-5 0.0 9 0.0 0.0 0.0 8.724960454116742E-5 0.0 10-11 0.0 0.0 0.0 8.724960454116742E-5 0.0 12-13 0.0 0.0 0.0 8.724960454116742E-5 0.0 14-15 0.0 0.0 0.0 8.724960454116742E-5 0.0 16-17 0.0 0.0 0.0 2.6174881362350225E-4 0.0 18-19 0.0 0.0 0.0 2.6174881362350225E-4 0.0 20-21 0.0 0.0 0.0 2.6174881362350225E-4 0.0 22-23 0.0 0.0 0.0 7.41621638599923E-4 0.0 24-25 0.0 0.0 0.0 0.002093990508988018 0.0 26-27 0.0 0.0 0.0 0.00423160582024662 0.0 28-29 0.0 0.0 0.0 0.010077329324504836 0.0 30-31 0.0 0.0 0.0 0.02386276684200929 0.0 32-33 0.0 0.0 0.0 0.04467179752507772 0.0 34-35 0.0 0.0 0.0 0.0749910351031334 0.0 36-37 0.0 0.0 0.0 0.12642467698015158 0.0 38-39 0.0 0.0 0.0 0.19447936852226216 0.0 40-41 0.0 0.0 0.0 0.2685542827777133 0.0 42-43 0.0 0.0 0.0 0.3346894830199182 0.0 44-45 0.0 0.0 0.0 0.41491549439552167 0.0 46-47 0.0 0.0 0.0 0.5245009976992279 0.0 48-49 0.0 0.0 0.0 0.6365731147323574 0.0 50-51 0.0 0.0 0.0 0.7427995082612289 0.0 52-53 0.0 0.0 0.0 0.845797666422077 0.0 54-55 0.0 0.0 0.0 0.9682961111978761 0.0 56-57 0.0 0.0 0.0 1.1273957650786948 0.0 58-59 0.0 0.0 0.0 1.3016768501496767 0.0 60-61 0.0 0.0 0.0 1.4828506539794108 0.0 62-63 0.0 0.0 0.0 1.6616687184865335 0.0 64-65 0.0 0.0 0.0 1.8474231265546788 0.0 66-67 0.0 0.0 0.0 2.074228473559444 0.0 68-69 0.0 0.0 0.0 2.3467089885415096 0.0 70-71 0.0 0.0 0.0 2.62773996476861 0.0 72-73 0.0 0.0 0.0 2.9176267758566388 0.0 74-75 0.0 0.0 0.0 3.2224768941234774 0.0 76-77 0.0 0.0 0.0 3.5499246599664787 0.0 78-79 0.0 0.0 0.0 3.923440217007216 0.0 80-81 0.0 0.0 0.0 4.32352327863074 0.0 82-83 0.0 0.0 0.0 4.756717565177636 0.0 84-85 0.0 0.0 0.0 5.205616780541942 0.0 86-87 0.0 0.0 0.0 5.69569780924968 0.0 88-89 0.0 0.0 0.0 6.243974324186375 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2680 0.0 55.475746 1 GTATCAA 4580 0.0 39.82533 1 ATCAACG 5580 0.0 32.347668 3 TCAACGC 5600 0.0 32.232143 4 TATCAAC 5730 0.0 31.500875 2 CAACGCA 5720 0.0 31.4729 5 AACGCAG 5785 0.0 31.037165 6 ACGCAGA 6360 0.0 28.305819 7 CGCAGAG 6345 0.0 28.148148 8 GTACATG 3500 0.0 25.785715 1 TACATGG 3520 0.0 24.964489 2 GCAGAGT 7160 0.0 24.877792 9 GAGTACT 4980 0.0 23.60693 12-13 CAGAGTA 6765 0.0 23.20584 10-11 AGAGTAC 6510 0.0 22.509602 10-11 ACATGGG 3670 0.0 21.61444 3 GTACTTT 5505 0.0 21.183016 14-15 AGTACTT 5285 0.0 21.03122 12-13 CATGGGG 1940 0.0 20.07732 4 ATCGCGT 60 0.0047649187 19.791668 40-41 >>END_MODULE