FastQCFastQC Report
Fri 10 Feb 2017
SRR2031814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146137
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65560.5720084073718936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65510.5715721593491877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.32046779747970794No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT27360.23871491802463407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27850.059.6294751
GTATCAA48150.039.7274361
ATCAACG59450.031.7990743
TCAACGC59600.031.7987384
TATCAAC61000.031.1467972
CAACGCA61150.030.992725
AACGCAG61650.030.9724986
ACGCAGA68450.027.6874317
CGCAGAG68800.027.3394628
CTATACG700.00839253927.1422084
GTACATG34350.025.073361
GCAGAGT76600.024.2455049
GAGTACT55200.024.00757812-13
TACATGG35850.023.8488032
CAGAGTA73250.023.05235110-11
ACATGGG35100.023.005153
GTACTTT60700.021.91051514-15
AGAGTAC69500.021.6649510-11
GTGTACG1100.002877759321.5903939
GTATAGG2308.853749E-820.6886861
Produced by FastQC (version 0.11.5)