FastQCFastQC Report
Fri 10 Feb 2017
SRR2031813_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031813_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161792
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.5952086629746836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.5346370648734177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.30471222310126583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.26330102848101267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.1854232594936709No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.11434434335443039No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.051.0185171
ATAGGAC550.002573104634.5454563
ACCAATC550.002573104634.5454567
GTATCAA8650.034.0462461
GGTATAG650.00582943229.230771
AACGCAG10050.028.358216
TATCAAC9950.028.1658292
CAACGCA9950.028.1658295
TCAACGC9850.027.9695434
GCTCTTA856.383222E-427.9411751
ATCAACG10050.027.4129353
ACGCAGA10600.026.438687
CGCAGAG10450.025.4545448
GAGTACT7600.024.062512-13
GTACACT1000.001647530623.754
TATGAGC1000.001647530623.753
CAGAGTA11100.023.53603610-11
GCAGAGT11750.023.446819
GTACATG5250.021.7142871
TACATGG5050.021.6336632
Produced by FastQC (version 0.11.5)