##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031813_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 161792 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45145619066456 34.0 31.0 34.0 31.0 34.0 2 32.57330399525316 34.0 31.0 34.0 31.0 34.0 3 32.63355419303797 34.0 33.0 34.0 31.0 34.0 4 35.79666485363924 37.0 37.0 37.0 35.0 37.0 5 35.82905211629747 37.0 37.0 37.0 35.0 37.0 6 35.91550880142405 37.0 37.0 37.0 35.0 37.0 7 35.94954015031646 37.0 37.0 37.0 35.0 37.0 8 35.951023536392405 37.0 37.0 37.0 35.0 37.0 9 37.76953125 39.0 39.0 39.0 37.0 39.0 10-11 37.814187351661396 39.0 39.0 39.0 36.5 39.0 12-13 37.777402467365505 39.0 39.0 39.0 37.0 39.0 14-15 39.324138399920884 41.0 40.0 41.0 37.0 41.0 16-17 39.26573316356804 41.0 40.0 41.0 37.0 41.0 18-19 39.29132157337817 41.0 40.0 41.0 37.0 41.0 20-21 39.23369202432753 41.0 40.0 41.0 36.5 41.0 22-23 39.13266107100475 41.0 40.0 41.0 36.0 41.0 24-25 39.092597285403485 41.0 40.0 41.0 36.0 41.0 26-27 38.95138820213607 41.0 39.0 41.0 35.0 41.0 28-29 38.79524945609177 41.0 39.0 41.0 35.0 41.0 30-31 38.58555120154272 41.0 38.5 41.0 35.0 41.0 32-33 38.38851735561708 40.5 38.0 41.0 35.0 41.0 34-35 38.15399092662183 40.0 38.0 41.0 34.5 41.0 36-37 37.981491668314874 40.0 38.0 41.0 34.0 41.0 38-39 37.74091426028481 40.0 38.0 41.0 33.0 41.0 40-41 37.60801522943038 40.0 38.0 41.0 33.0 41.0 42-43 37.38478107693829 40.0 37.5 41.0 32.5 41.0 44-45 37.43111834948576 40.0 38.0 41.0 32.5 41.0 46-47 37.19888189774525 40.0 38.0 41.0 31.0 41.0 48-49 36.924684162381325 40.0 37.5 41.0 29.5 41.0 50-51 35.850193458267405 39.0 36.0 40.0 27.5 40.5 52-53 35.838320188884495 39.5 36.0 40.0 27.0 40.5 54-55 36.54952655261076 40.0 37.0 41.0 28.0 41.0 56-57 36.533611056170884 40.0 36.5 41.0 27.5 41.0 58-59 36.40058531942247 40.0 36.0 41.0 27.0 41.0 60-61 36.191718379153485 40.0 35.5 41.0 26.5 41.0 62-63 35.936643962618675 39.5 35.0 41.0 26.0 41.0 64-65 35.61307110363924 39.0 35.0 41.0 25.0 41.0 66-67 35.342727081685126 39.0 35.0 41.0 25.0 41.0 68-69 35.00440689280063 37.5 35.0 41.0 23.5 41.0 70-71 34.635269358188296 37.0 35.0 40.0 23.5 41.0 72-73 34.223469021954116 36.5 35.0 39.0 22.0 41.0 74-75 33.86991322191456 36.0 35.0 39.0 21.5 41.0 76-77 33.48393925534019 35.5 34.5 39.0 20.0 40.5 78-79 33.10398227353639 35.0 34.0 37.0 20.0 39.0 80-81 32.74847334849683 35.0 34.0 37.0 19.0 39.0 82-83 32.45182703718355 35.0 34.0 36.5 19.0 39.0 84-85 32.12278110166139 35.0 34.0 36.0 18.0 37.0 86-87 31.876121810719937 35.0 34.0 36.0 17.5 37.0 88-89 31.654352501977847 35.0 34.0 35.5 17.0 37.0 90-91 31.461428871637658 35.0 33.5 35.0 15.0 36.0 92-93 31.311659414556964 35.0 33.0 35.0 15.0 36.0 94-95 31.126625543908226 35.0 33.0 35.0 12.0 36.0 96-97 31.017828446400316 35.0 33.0 35.0 11.0 36.0 98-99 30.879576864121837 35.0 33.0 35.0 10.0 35.0 100-101 29.452979751780063 34.0 29.0 35.0 9.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.019630898334789038 1101 2 -0.03827991256004992 1101 3 0.33140497425409876 1101 4 0.047991376161299115 1101 5 -0.13971548832821412 1101 6 -0.26055121897414324 1101 7 -0.474808365603657 1101 8 0.014999334014554222 1101 9 0.15314770787210108 1101 10-11 0.16310427372724234 1101 12-13 0.0011181799373432 1101 14-15 0.047558819293328725 1101 16-17 0.14765835360376656 1101 18-19 0.3033255847036429 1101 20-21 0.3089002948667243 1101 22-23 0.31631006005444107 1101 24-25 0.4015755197610176 1101 26-27 0.4974370949770943 1101 28-29 0.45325505871970506 1101 30-31 0.3000656918950284 1101 32-33 0.5418639217623706 1101 34-35 0.7501763048817054 1101 36-37 0.4306099513680124 1101 38-39 0.6894692628738284 1101 40-41 0.533515046286908 1101 42-43 0.8219558743474309 1101 44-45 1.0629499466541574 1101 46-47 0.8986400959704284 1101 48-49 1.5835924467973257 1101 50-51 1.5341609652544719 1101 52-53 1.1145928625408104 1101 54-55 1.1479991784369261 1101 56-57 1.2806955436594194 1101 58-59 1.2792916460120551 1101 60-61 1.3954867724356674 1101 62-63 0.9427597726243135 1101 64-65 1.2454598961172394 1101 66-67 1.0039211547263633 1101 68-69 0.8722077079029233 1101 70-71 1.1934808514825264 1101 72-73 1.6790080808801378 1101 74-75 1.0600685281738862 1101 76-77 1.314562881745914 1101 78-79 1.2785237447956987 1101 80-81 1.1813663432665038 1101 82-83 0.9905782942735897 1101 84-85 1.434057708900177 1101 86-87 1.171512775140755 1101 88-89 0.8904753366161202 1101 90-91 1.5507062374876917 1101 92-93 1.3249290278973547 1101 94-95 1.1441866087382806 1101 96-97 1.2469750391318364 1101 98-99 1.0863170769823292 1101 100-101 1.382279014733495 1102 1 0.4526263526109844 1102 2 0.48227494242103575 1102 3 0.6103457056538488 1102 4 0.17964333076407257 1102 5 0.5640676138155385 1102 6 0.37601876841551984 1102 7 0.41283350072673386 1102 8 0.4549741133335985 1102 9 0.4177126511255693 1102 10-11 0.44437791811564864 1102 12-13 0.508053867200907 1102 14-15 0.5938387688519597 1102 16-17 0.7257794128723631 1102 18-19 0.7888236931525725 1102 20-21 0.7964160577923209 1102 22-23 0.7142293538753748 1102 24-25 0.7095200342629013 1102 26-27 1.098382870513035 1102 28-29 1.0265841886062148 1102 30-31 1.1609736364095298 1102 32-33 1.0635537073865748 1102 34-35 0.7716138836963324 1102 36-37 0.7487057899556575 1102 38-39 0.951196879519479 1102 40-41 0.8931619567534952 1102 42-43 1.2467982450914477 1102 44-45 1.322975167335109 1102 46-47 1.5451722523386522 1102 48-49 1.7823314127494072 1102 50-51 1.3930512552922991 1102 52-53 1.9294730643062579 1102 54-55 2.076183289407922 1102 56-57 1.9315782674929665 1102 58-59 2.1156724782945275 1102 60-61 2.1989546160674465 1102 62-63 1.861170869723935 1102 64-65 1.8389655707704549 1102 66-67 1.8633158583833662 1102 68-69 1.852913886969752 1102 70-71 1.827465719073956 1102 72-73 1.9433208173240217 1102 74-75 1.9587444424235656 1102 76-77 1.5592665387446516 1102 78-79 1.4053837700163783 1102 80-81 1.4780246030395219 1102 82-83 1.6446766549293272 1102 84-85 1.6323553129354806 1102 86-87 1.2767460664396282 1102 88-89 1.4733883252668107 1102 90-91 1.2374653799845383 1102 92-93 1.4812972498393435 1102 94-95 1.4873139731771232 1102 96-97 1.4395979899515048 1102 98-99 1.7550434325939257 1102 100-101 1.2149397965746012 1103 1 -0.569741350607778 1103 2 -0.401958554578421 1103 3 -0.6854535305709177 1103 4 -0.5928062055152523 1103 5 -0.7715024898396692 1103 6 -0.5262180019063578 1103 7 -0.2660535696496993 1103 8 -0.5277863885758407 1103 9 -0.6912889855356426 1103 10-11 -0.3216068063797195 1103 12-13 -0.6157868311078829 1103 14-15 -0.5771650500241989 1103 16-17 -0.7739205325722622 1103 18-19 -0.569741500519001 1103 20-21 -0.6980465717766933 1103 22-23 -0.6159943231568121 1103 24-25 -0.2822693819946025 1103 26-27 -0.6019990171028908 1103 28-29 -0.4522210487096672 1103 30-31 -0.8298610608081489 1103 32-33 -0.8237621682271694 1103 34-35 -1.062810557111078 1103 36-37 -0.6294993382494809 1103 38-39 -0.6633966829464271 1103 40-41 -0.597399963595656 1103 42-43 -0.6546747499985699 1103 44-45 -0.7985196499043852 1103 46-47 -1.4081556254573186 1103 48-49 -1.2631404912331305 1103 50-51 -1.5399001904251008 1103 52-53 -1.4645258445862694 1103 54-55 -1.315551571475872 1103 56-57 -0.7927261514923032 1103 58-59 -0.9668425244332468 1103 60-61 -1.568994101881266 1103 62-63 -1.2101875590813052 1103 64-65 -0.8427856567254537 1103 66-67 -0.9520142016275486 1103 68-69 -1.5805012794241335 1103 70-71 -1.2272260430646185 1103 72-73 -1.1234276824031966 1103 74-75 -0.8528485744885899 1103 76-77 -0.8512899260671389 1103 78-79 -1.0465256680632748 1103 80-81 -1.2891603396773377 1103 82-83 -1.0620609337231528 1103 84-85 -0.6277374311998116 1103 86-87 -0.2820100710399167 1103 88-89 -0.49401483894486375 1103 90-91 -0.7132165621867657 1103 92-93 -0.8805412662544896 1103 94-95 -0.6408093219674527 1103 96-97 -0.6490544923179975 1103 98-99 -0.36868815496744745 1103 100-101 -0.5896428548165566 1104 1 0.49408843662626367 1104 2 0.6051336440031392 1104 3 0.35709966091844336 1104 4 0.37882500015850695 1104 5 0.6469372264723887 1104 6 0.4312288066042811 1104 7 0.322598912619803 1104 8 0.36583063270075655 1104 9 0.17395924141471397 1104 10-11 0.3521520588685121 1104 12-13 0.39839756605523746 1104 14-15 0.2845370776353775 1104 16-17 0.40338443196674234 1104 18-19 0.4834499888426933 1104 20-21 0.5643647757410406 1104 22-23 0.5613106413821995 1104 24-25 0.6184398378635549 1104 26-27 0.546937153109873 1104 28-29 0.5938782420704811 1104 30-31 0.3332595065677353 1104 32-33 0.5521243565955274 1104 34-35 0.5258419251584172 1104 36-37 0.14709640643137334 1104 38-39 0.21603594116704983 1104 40-41 0.1366425895958301 1104 42-43 -0.005738579785806053 1104 44-45 0.14828886073391345 1104 46-47 0.15212806248595 1104 48-49 -0.34470077492107976 1104 50-51 -0.17465196347475143 1104 52-53 -0.20211449706854268 1104 54-55 -0.049594124667365236 1104 56-57 -0.07641409475471761 1104 58-59 -0.555495006702742 1104 60-61 -0.522894811101132 1104 62-63 -0.08252798461321476 1104 64-65 -0.24704097587439833 1104 66-67 -0.033574485315490676 1104 68-69 -0.40319631488512897 1104 70-71 -0.14566575937667636 1104 72-73 -0.02413690226135401 1104 74-75 -0.11171382271554364 1104 76-77 -0.09597077713096525 1104 78-79 0.3896445447026835 1104 80-81 0.11154888018081976 1104 82-83 -0.11525242308485417 1104 84-85 -0.04617714751186952 1104 86-87 0.30309631193880193 1104 88-89 0.3074036007714511 1104 90-91 -0.2380392572222263 1104 92-93 -0.44437105348853123 1104 94-95 -0.4280433645206436 1104 96-97 -0.6632388894811179 1104 98-99 -0.31904276489652617 1104 100-101 -0.06482015978109601 1105 1 0.380900780885419 1105 2 0.3261418862879779 1105 3 0.4790567243648667 1105 4 0.7076059087266557 1105 5 0.6650031647510914 1105 6 0.7256029679997198 1105 7 0.3986962865895194 1105 8 0.4247246330841179 1105 9 0.4590847924977126 1105 10-11 0.49224694098466415 1105 12-13 0.45166093580797195 1105 14-15 0.5124458374590333 1105 16-17 0.5607066477995986 1105 18-19 0.6487509280798065 1105 20-21 0.602653064029326 1105 22-23 0.6490526386986559 1105 24-25 0.5813495560924267 1105 26-27 0.6124681095982751 1105 28-29 0.7242453112673317 1105 30-31 0.7121170875529828 1105 32-33 0.6004040192368905 1105 34-35 0.8282666903491389 1105 36-37 0.9831132743631343 1105 38-39 0.6030160063386063 1105 40-41 0.6581827467742798 1105 42-43 0.823617371910565 1105 44-45 0.6870084313683762 1105 46-47 0.7797876369540333 1105 48-49 0.8468844273024203 1105 50-51 0.815452502693546 1105 52-53 0.6469366817698727 1105 54-55 0.8678153060399367 1105 56-57 1.1024722383869374 1105 58-59 1.2843315219535754 1105 60-61 0.7069067990196274 1105 62-63 0.8838319923850548 1105 64-65 1.239589271394152 1105 66-67 1.4015175215850277 1105 68-69 1.2242236582795272 1105 70-71 0.9298565714648035 1105 72-73 1.055171129174333 1105 74-75 1.0706467293258584 1105 76-77 1.3703891998673043 1105 78-79 0.9683775330101412 1105 80-81 1.1861846861996028 1105 82-83 0.8088658441185217 1105 84-85 0.8405153960955687 1105 86-87 0.6636483533419124 1105 88-89 0.33747356435205234 1105 90-91 0.40310987062902726 1105 92-93 1.1159126344547303 1105 94-95 1.0477089835721287 1105 96-97 1.0916250169785293 1105 98-99 0.963255490805988 1105 100-101 0.9974761791109863 1106 1 0.07376711660889157 1106 2 -0.26633834904939846 1106 3 -0.28206664390135927 1106 4 -0.28205211664565866 1106 5 -0.11395436420643534 1106 6 0.03609202848877402 1106 7 -0.12290694929942703 1106 8 0.11441942992176735 1106 9 -0.11739985541550624 1106 10-11 -0.22325682809052694 1106 12-13 0.02793874422863496 1106 14-15 -0.0804940411951307 1106 16-17 -0.05949007953999086 1106 18-19 -0.20869069721896238 1106 20-21 -0.06828828548345456 1106 22-23 -0.02234835413090508 1106 24-25 0.06213646545076301 1106 26-27 0.20115628757216797 1106 28-29 0.0781017186951729 1106 30-31 0.033867409303304896 1106 32-33 -0.005336925789762859 1106 34-35 0.2635029470139756 1106 36-37 0.14260950528793614 1106 38-39 0.25844439390984775 1106 40-41 -0.0984131348216053 1106 42-43 0.15846752818929843 1106 44-45 0.5587679459850321 1106 46-47 0.47366518797444 1106 48-49 0.3522162897771395 1106 50-51 0.10592409673657244 1106 52-53 0.6166002228619831 1106 54-55 0.7110079885183325 1106 56-57 0.7023527311245701 1106 58-59 0.8062794903301125 1106 60-61 0.740529707642537 1106 62-63 0.6245931617176552 1106 64-65 0.5349101024292722 1106 66-67 0.5770782864315009 1106 68-69 0.6989892401879629 1106 70-71 0.8694594396391011 1106 72-73 0.836334946052439 1106 74-75 0.8561541755475872 1106 76-77 0.7199939066148744 1106 78-79 0.27877466483584357 1106 80-81 0.3596310917888701 1106 82-83 0.28039094064361336 1106 84-85 0.4289277880793847 1106 86-87 0.3751762077269092 1106 88-89 0.41935902123751134 1106 90-91 0.5829912826532997 1106 92-93 0.3948241990805812 1106 94-95 0.4946661448150067 1106 96-97 0.30014220763857935 1106 98-99 0.7753992672354784 1106 100-101 1.1332548148896215 1107 1 0.3596147890111894 1107 2 0.2965534743466236 1107 3 0.29883238237582077 1107 4 0.45530949466553494 1107 5 0.26326318948170524 1107 6 0.5020117297025948 1107 7 0.34234581847434953 1107 8 0.2487398247463659 1107 9 0.30690319913964714 1107 10-11 0.15169315190146904 1107 12-13 0.09503224829692414 1107 14-15 -0.03781030103240113 1107 16-17 0.1217824294636145 1107 18-19 0.19593782085493672 1107 20-21 0.05782882802861877 1107 22-23 0.29271100294526065 1107 24-25 0.4612294359723066 1107 26-27 0.19622098011584654 1107 28-29 0.0766344651270785 1107 30-31 -0.1341998175874508 1107 32-33 0.3665451603780312 1107 34-35 0.42043238545601014 1107 36-37 -0.0347280964194141 1107 38-39 0.3955659091826291 1107 40-41 0.33529368123816283 1107 42-43 0.3195986473036143 1107 44-45 0.3913009591903176 1107 46-47 0.3745588787841001 1107 48-49 0.5827084866558891 1107 50-51 0.34488634830385934 1107 52-53 0.6750235922097261 1107 54-55 -0.07497307057196423 1107 56-57 0.10304634190221407 1107 58-59 0.31941807909895203 1107 60-61 0.6466921726285335 1107 62-63 0.2827897736957823 1107 64-65 0.5757931223038852 1107 66-67 0.43395437019834304 1107 68-69 0.6297615491115351 1107 70-71 0.12196044003925977 1107 72-73 0.22082207423704148 1107 74-75 0.7709867752541371 1107 76-77 0.7954907720277049 1107 78-79 0.99359392587359 1107 80-81 0.6405434567348465 1107 82-83 0.39663743924305805 1107 84-85 0.6608872385262359 1107 86-87 0.7324524515577764 1107 88-89 0.9473702257192969 1107 90-91 0.885378904368654 1107 92-93 0.7956511965462312 1107 94-95 0.8090585684511282 1107 96-97 1.1846960291671955 1107 98-99 1.0127608785466684 1107 100-101 0.5412846935077376 1108 1 0.5712160962007289 1108 2 0.5635936237397203 1108 3 0.41776328807143415 1108 4 0.4194784955992361 1108 5 0.707474207222134 1108 6 0.583711076107825 1108 7 0.6577052955985323 1108 8 0.6883990217585065 1108 9 0.6606613690742904 1108 10-11 0.6474914712291948 1108 12-13 0.6167992884463231 1108 14-15 0.6805603805735743 1108 16-17 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-1.3028579882253801 2316 80-81 -0.8905235155085975 2316 82-83 -0.8321631719104978 2316 84-85 -1.108487177907005 2316 86-87 -1.2058380161444617 2316 88-89 -1.0330219311434448 2316 90-91 -0.8901070475878896 2316 92-93 -1.301836283294186 2316 94-95 -1.50997207410893 2316 96-97 -1.2075881572346425 2316 98-99 -1.376425098874602 2316 100-101 -1.2022430206082113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 193.0 8 378.0 9 244.0 10 253.0 11 272.0 12 318.0 13 311.0 14 294.0 15 304.0 16 444.0 17 639.0 18 779.0 19 1059.0 20 1426.0 21 1796.0 22 1838.0 23 1701.0 24 1516.0 25 1326.0 26 1222.0 27 1181.0 28 1307.0 29 1388.0 30 1613.0 31 1848.0 32 2298.0 33 3069.0 34 4454.0 35 8008.0 36 14491.0 37 31463.0 38 53192.0 39 21137.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31178303006329 20.246983781645568 14.390081091772153 22.051152096518987 2 15.458737143987342 22.101834454113924 40.16700454905063 22.2724238528481 3 17.56267306170886 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20.672530162183545 28.37223101265823 31.196536293512654 19.758702531645568 24-25 20.34927561313291 28.43372972705696 31.21569669699367 20.001297962816455 26-27 20.387287381329113 28.47915842563291 31.25339942642405 19.880154766613924 28-29 20.39439527294304 28.77212717563291 31.121748912183545 19.711728639240505 30-31 20.51337519778481 28.33360116693038 31.30779024920886 19.84523338607595 32-33 20.104207871835442 28.55425484572785 31.254326542721518 20.08721073971519 34-35 20.522028283227847 28.885544402689874 30.81580053401899 19.776626780063292 36-37 20.41108336629747 28.768109671677216 30.95795836629747 19.862848595727847 38-39 20.469491693037973 28.7245352056962 30.839596518987346 19.966376582278482 40-41 20.546442345727847 28.734733484968356 30.50768888449367 20.211135284810126 42-43 20.350202729430382 28.947970233386076 30.490382713607595 20.21144432357595 44-45 20.406138746044302 28.90655903876582 30.37232990506329 20.314972310126585 46-47 20.281905162183545 28.78541584256329 30.136224287974684 20.796454707278482 48-49 20.344330992879748 29.00915990901899 29.765686807753166 20.8808222903481 50-51 20.309718651107595 29.34384889240506 29.450467266613924 20.895965189873415 52-53 20.725375791139243 29.142046578322784 29.27060670490506 20.86197092563291 54-55 20.42591722705696 29.027702234968356 29.477353639240505 21.06902689873418 56-57 20.371526404272153 29.08580152294304 29.48878807357595 21.05388399920886 58-59 20.585690268987342 28.910885581487346 29.59509740901899 20.90832674050633 60-61 20.323934434335442 28.71742731408228 29.756106606012654 21.202531645569618 62-63 20.27263399920886 28.828681269778482 29.801535304588604 21.09714942642405 64-65 20.19012064873418 28.82528184335443 29.69615308544304 21.288444422468356 66-67 20.137275019778482 28.64418512658228 29.78855567642405 21.42998417721519 68-69 20.353911194620252 28.64325801028481 29.624765130537973 21.37806566455696 70-71 20.086592662183545 28.727316554588604 29.478280755537973 21.707810027689874 72-73 20.30044748813291 28.603701048259495 29.34539408623418 21.750457377373415 74-75 20.296120945411392 28.531695015822784 29.41121934335443 21.760964695411392 76-77 20.15612638449367 28.400971617879744 29.507330399525316 21.935571598101266 78-79 20.216697982594937 28.398190268987346 29.30460096914557 22.080510779272153 80-81 20.244202432753163 28.687141515031644 29.24248417721519 21.826171875 82-83 20.28901305379747 28.403134889240505 29.195201246044306 22.11265081091772 84-85 20.35792869857595 28.34256329113924 29.1571894778481 22.142318532436708 86-87 20.293030557753163 28.39015526107595 29.019049149525316 22.297765031645568 88-89 20.2460566653481 28.227600870253166 29.250519185126585 22.275823279272153 90-91 20.30353787579114 28.140142899525316 29.175422765031644 22.3808964596519 92-93 20.45249456091772 27.75662579113924 29.31819867484177 22.472680973101266 94-95 20.17837717563291 27.68585591376582 29.4053476068038 22.73041930379747 96-97 20.1836308346519 27.616013152689874 29.367644877373415 22.83271113528481 98-99 20.210208168512658 27.6611328125 29.27060670490506 22.85805231408228 100-101 20.48308939873418 27.4101933346519 29.52525464794304 22.581462618670887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 555.0 1 328.5 2 119.5 3 148.5 4 129.0 5 111.0 6 156.5 7 262.0 8 382.5 9 438.5 10 358.5 11 258.0 12 239.5 13 245.0 14 293.5 15 316.0 16 314.5 17 348.0 18 381.0 19 416.0 20 464.5 21 559.0 22 683.0 23 827.5 24 1001.0 25 1236.5 26 1474.0 27 1767.5 28 2274.0 29 2696.0 30 2980.5 31 3396.0 32 3786.0 33 4035.5 34 4174.5 35 4282.0 36 4466.0 37 4758.5 38 5085.5 39 5324.5 40 5571.5 41 5647.0 42 5543.0 43 5477.5 44 5428.0 45 5598.0 46 5746.5 47 5649.0 48 5643.0 49 5645.5 50 5486.0 51 5182.0 52 4767.5 53 4341.0 54 4035.5 55 3648.0 56 3148.0 57 2736.5 58 2398.5 59 2029.5 60 1626.0 61 1297.5 62 1024.5 63 793.0 64 600.0 65 434.0 66 302.0 67 233.0 68 193.5 69 150.5 70 112.0 71 88.0 72 75.5 73 66.0 74 55.5 75 44.5 76 32.0 77 25.0 78 19.5 79 16.5 80 12.5 81 14.0 82 16.5 83 11.5 84 7.5 85 7.5 86 4.0 87 2.0 88 3.0 89 1.0 90 1.5 91 1.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 161792.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.83192794831685 #Duplication Level Percentage of deduplicated Percentage of total 1 77.78322669648125 55.09535908952756 2 15.105723557090874 21.399350452061068 3 4.204239871230966 8.93383246909418 4 1.4583037994108576 4.1317787858650625 5 0.6005656075982011 2.126960992281645 6 0.308170673880058 1.3096993780833908 7 0.17694222943395382 0.8773211472396255 8 0.09841089691213231 0.5576506847527514 9 0.04373810843068638 0.27882490904622426 >10 0.20775594138820985 2.553781766129781 >50 0.003976190197777927 0.19292423405449974 >100 0.006958332846111372 1.2554157274422373 >500 0.0019880950988889635 1.2871003644219912 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 963 0.5952086629746836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.5346370648734177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 493 0.30471222310126583 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 426 0.26330102848101267 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.1854232594936709 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 185 0.11434434335443039 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.180775316455697E-4 0.0 10-11 0.0 0.0 0.0 6.180775316455697E-4 0.0 12-13 0.0 0.0 0.0 6.180775316455697E-4 0.0 14-15 0.0 0.0 0.0 6.180775316455697E-4 0.0 16-17 0.0 0.0 0.0 0.0012361550632911393 0.0 18-19 0.0 0.0 0.0 0.0012361550632911393 0.0 20-21 0.0 0.0 0.0 0.001854232594936709 0.0 22-23 0.0 0.0 0.0 0.005562697784810127 0.0 24-25 0.0 0.0 0.0 0.01545193829113924 0.0 26-27 0.0 0.0 0.0 0.0346123417721519 0.0 28-29 0.0 0.0 0.0 0.07633257515822785 0.0 30-31 0.0 0.0 0.0 0.14370302610759494 0.0 32-33 0.0 0.0 0.0 0.22127175632911394 0.0 34-35 0.0 0.0 0.0 0.33252571202531644 0.0 36-37 0.0 0.0 0.0 0.479628164556962 0.0 38-39 0.0 0.0 0.0 0.6567073773734178 0.0 40-41 0.0 0.0 0.0 0.8714893196202531 0.0 42-43 0.0 0.0 0.0 1.0494956487341773 6.180775316455697E-4 44-45 0.0 0.0 0.0 1.2166856210443038 6.180775316455697E-4 46-47 0.0 0.0 0.0 1.388511174841772 6.180775316455697E-4 48-49 0.0 0.0 0.0 1.5804242484177216 6.180775316455697E-4 50-51 0.0 0.0 0.0 1.7834627175632911 6.180775316455697E-4 52-53 0.0 0.0 0.0 1.9410724881329113 6.180775316455697E-4 54-55 0.0 0.0 0.0 2.142565763449367 6.180775316455697E-4 56-57 0.0 0.0 0.0 2.39814082278481 6.180775316455697E-4 58-59 0.0 0.0 0.0 2.6657683939873418 6.180775316455697E-4 60-61 0.0 0.0 0.0 2.9318507713607596 6.180775316455697E-4 62-63 0.0 0.0 0.0 3.191134295886076 6.180775316455697E-4 64-65 0.0 0.0 0.0 3.4535082080696204 6.180775316455697E-4 66-67 0.0 0.0 0.0 3.792523734177215 6.180775316455697E-4 68-69 6.180775316455697E-4 0.0 0.0 4.1578075553797476 6.180775316455697E-4 70-71 6.180775316455697E-4 0.0 0.0 4.576864121835443 6.180775316455697E-4 72-73 6.180775316455697E-4 0.0 0.0 4.996847804588608 6.180775316455697E-4 74-75 6.180775316455697E-4 0.0 0.0 5.452370945411392 6.180775316455697E-4 76-77 6.180775316455697E-4 0.0 0.0 5.9051127373417724 6.180775316455697E-4 78-79 6.180775316455697E-4 0.0 0.0 6.3952482199367084 6.180775316455697E-4 80-81 6.180775316455697E-4 0.0 0.0 6.935447982594937 6.180775316455697E-4 82-83 6.180775316455697E-4 0.0 0.0 7.490481606012658 6.180775316455697E-4 84-85 6.180775316455697E-4 0.0 0.0 8.098978935917721 6.180775316455697E-4 86-87 6.180775316455697E-4 0.0 0.0 8.771447290348101 6.180775316455697E-4 88-89 6.180775316455697E-4 0.0 0.0 9.49490704113924 6.180775316455697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 540 0.0 51.018517 1 ATAGGAC 55 0.0025731046 34.545456 3 ACCAATC 55 0.0025731046 34.545456 7 GTATCAA 865 0.0 34.046246 1 GGTATAG 65 0.005829432 29.23077 1 AACGCAG 1005 0.0 28.35821 6 TATCAAC 995 0.0 28.165829 2 CAACGCA 995 0.0 28.165829 5 TCAACGC 985 0.0 27.969543 4 GCTCTTA 85 6.383222E-4 27.941175 1 ATCAACG 1005 0.0 27.412935 3 ACGCAGA 1060 0.0 26.43868 7 CGCAGAG 1045 0.0 25.454544 8 GAGTACT 760 0.0 24.0625 12-13 GTACACT 100 0.0016475306 23.75 4 TATGAGC 100 0.0016475306 23.75 3 CAGAGTA 1110 0.0 23.536036 10-11 GCAGAGT 1175 0.0 23.44681 9 GTACATG 525 0.0 21.714287 1 TACATGG 505 0.0 21.633663 2 >>END_MODULE