FastQCFastQC Report
Fri 10 Feb 2017
SRR2031813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161792
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13420.8294600474683546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.7361303401898734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.7095530063291139No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.3498318829113924No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.22312598892405064No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT3440.21261867088607594RNA PCR Primer, Index 39 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.20829212816455694No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT2390.14772053006329114RNA PCR Primer, Index 39 (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.13968552215189875No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.13165051424050633No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG1910.11805280854430379RNA PCR Primer, Index 39 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC301.2979872E-463.33213
GGTATCA7000.048.946971
GTATCAA10850.032.8944741
CTGGTGT753.0672038E-431.6660464
ATGGGAC650.00582998829.2301987
TCAACGC12100.028.6564664
TATCAAC12550.028.38592
CAACGCA12250.028.3055695
AACGCAG12300.028.1905066
ATCAACG12350.028.0763763
GTTCTAT700.00829592827.1927621
TTAGGTT700.00837143927.1423284
ACTGACC700.00837143927.1423288
CTTTTCG350.00838361327.14232846-47
AAACGAC350.00838361327.14232846-47
CATGGGG2500.026.5994824
ACGCAGA13700.025.3097257
CGCAGAG13850.025.0356128
GAGTACT9600.024.2443212-13
GTCTGGC1000.001647718323.7495377
Produced by FastQC (version 0.11.5)