##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031813_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 161792 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86485116693038 34.0 33.0 34.0 31.0 34.0 2 32.983509691455694 34.0 33.0 34.0 31.0 34.0 3 33.03101513053797 34.0 33.0 34.0 31.0 34.0 4 36.40069966376582 37.0 37.0 37.0 35.0 37.0 5 36.40123739121835 37.0 37.0 37.0 35.0 37.0 6 36.479201691060126 37.0 37.0 37.0 35.0 37.0 7 36.42080572587025 37.0 37.0 37.0 35.0 37.0 8 36.473292869857595 37.0 37.0 37.0 35.0 37.0 9 38.26794279074367 39.0 39.0 39.0 37.0 39.0 10-11 38.28434656843355 39.0 39.0 39.0 37.0 39.0 12-13 38.293144902096515 39.0 39.0 39.0 37.0 39.0 14-15 39.83696968947785 41.0 40.0 41.0 38.0 41.0 16-17 39.79843873615506 41.0 40.0 41.0 37.5 41.0 18-19 39.863525390625 41.0 40.0 41.0 38.0 41.0 20-21 39.863658277294306 41.0 40.0 41.0 38.0 41.0 22-23 39.80941270272943 41.0 40.0 41.0 37.0 41.0 24-25 39.72789754746836 41.0 40.0 41.0 37.0 41.0 26-27 39.58518344541139 41.0 40.0 41.0 36.5 41.0 28-29 39.512343008306964 41.0 40.0 41.0 36.0 41.0 30-31 39.31250927116298 41.0 39.0 41.0 35.5 41.0 32-33 38.788812178599684 40.0 38.0 41.0 35.0 41.0 34-35 38.77976043314874 40.0 38.0 41.0 35.0 41.0 36-37 38.67969677116297 41.0 38.0 41.0 35.0 41.0 38-39 38.511703298061704 40.5 38.0 41.0 35.0 41.0 40-41 38.370228441455694 40.0 38.0 41.0 35.0 41.0 42-43 38.179205399525316 40.0 38.0 41.0 34.5 41.0 44-45 37.87546046776107 40.0 38.0 41.0 33.5 41.0 46-47 37.60202605814874 40.0 38.0 41.0 33.0 41.0 48-49 37.38200281843355 40.0 38.0 41.0 32.5 41.0 50-51 37.31091772151899 40.0 38.0 41.0 32.0 41.0 52-53 37.34129932258703 40.0 38.0 41.0 32.0 41.0 54-55 37.23111773140823 40.0 37.5 41.0 31.5 41.0 56-57 37.067107767998415 40.0 37.0 41.0 31.0 41.0 58-59 36.871405879153485 40.0 37.0 41.0 30.5 41.0 60-61 36.626291782041136 40.0 36.0 41.0 29.5 41.0 62-63 36.35402553896361 39.5 35.5 41.0 29.0 41.0 64-65 36.05718144284019 39.0 35.0 41.0 28.0 41.0 66-67 35.72716512559335 39.0 35.0 41.0 27.5 41.0 68-69 35.26951888844937 38.0 35.0 41.0 26.0 41.0 70-71 34.854241866099684 37.0 35.0 40.0 25.0 41.0 72-73 34.424384394778485 37.0 35.0 39.0 24.0 41.0 74-75 34.03740914260285 36.0 35.0 39.0 23.0 41.0 76-77 32.60994981210443 35.0 32.5 37.0 20.5 39.0 78-79 33.26682716079905 35.0 34.0 37.0 22.5 39.0 80-81 33.093923061708864 35.0 34.0 37.0 23.0 39.0 82-83 32.776722582080694 35.0 34.0 36.5 20.5 39.0 84-85 32.519534340387665 35.0 34.0 36.0 20.0 37.0 86-87 32.28590412381329 35.0 34.0 36.0 20.0 37.0 88-89 32.09128696103639 35.0 34.0 35.5 20.0 37.0 90-91 31.896595628955698 35.0 34.0 35.0 19.0 36.0 92-93 31.746640748615505 35.0 34.0 35.0 19.0 36.0 94-95 31.66167363034019 35.0 34.0 35.0 19.0 36.0 96-97 31.565856160996837 35.0 34.0 35.0 18.0 36.0 98-99 31.49674891218354 35.0 34.0 35.0 18.0 35.0 100-101 30.439548927017405 34.5 31.5 35.0 13.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12934563280286682 1101 2 0.2800800750911776 1101 3 0.14227266403656103 1101 4 0.0275406649316281 1101 5 -0.018082071797664412 1101 6 0.08031985165007427 1101 7 0.22455141613023244 1101 8 0.063017873169116 1101 9 0.016354219089002697 1101 10-11 0.027741769093367452 1101 12-13 -0.205760307025308 1101 14-15 -0.12384752632334539 1101 16-17 -0.28201918184755215 1101 18-19 -0.20331445141792415 1101 20-21 -0.05111140059020869 1101 22-23 -0.322340267844865 1101 24-25 -0.275149438380879 1101 26-27 0.15655737595949404 1101 28-29 -0.017455053606113324 1101 30-31 0.2373019045156184 1101 32-33 -0.11855062611803646 1101 34-35 0.065782403934314 1101 36-37 0.10142002703211972 1101 38-39 0.27229151595110324 1101 40-41 0.46589605691023195 1101 42-43 0.4725829709604312 1101 44-45 0.4054492759711579 1101 46-47 0.48487447004460194 1101 48-49 0.5497659211970785 1101 50-51 1.1006043467418252 1101 52-53 0.7356688758923582 1101 54-55 0.9884044166214849 1101 56-57 0.7910374609767956 1101 58-59 0.7360055970111503 1101 60-61 1.1999056374211676 1101 62-63 1.2098785336489541 1101 64-65 0.46008333327518613 1101 66-67 0.21216926187428697 1101 68-69 0.6610171581746798 1101 70-71 0.800475918929088 1101 72-73 0.8918929730280354 1101 74-75 0.7482287192139907 1101 76-77 0.5243473512985233 1101 78-79 1.0666847650593212 1101 80-81 0.8836615263271099 1101 82-83 0.7308237387011829 1101 84-85 0.9031324421718878 1101 86-87 0.7882368609177419 1101 88-89 1.2254230948464553 1101 90-91 1.2565252581420587 1101 92-93 0.5842920431412715 1101 94-95 0.9988480353287663 1101 96-97 0.8609772672190132 1101 98-99 0.41058103865306705 1101 100-101 0.5834974438930978 1102 1 0.3057642961067728 1102 2 0.1849981078780658 1102 3 0.4378590448688442 1102 4 0.06133637741586284 1102 5 0.17309068986689624 1102 6 0.16089992731211566 1102 7 0.01597638460437878 1102 8 0.0456156033078301 1102 9 -0.009496978893345442 1102 10-11 0.11292461903032347 1102 12-13 -0.10336434233425962 1102 14-15 -0.1423216751253662 1102 16-17 0.32573617754714945 1102 18-19 0.16484443887211597 1102 20-21 0.16566035224712294 1102 22-23 0.2881893664552635 1102 24-25 0.19048738381331987 1102 26-27 -0.03827238444403491 1102 28-29 0.21949324399792403 1102 30-31 0.7012363307451253 1102 32-33 0.8388264230622937 1102 34-35 0.18078870910455436 1102 36-37 0.45482481896149807 1102 38-39 0.6077896244000343 1102 40-41 0.42194902034024295 1102 42-43 0.37125888521011063 1102 44-45 0.5560797929951207 1102 46-47 0.5081405482287167 1102 48-49 0.7234103095955646 1102 50-51 0.4234290630091664 1102 52-53 0.49228930464393983 1102 54-55 0.6874586410855414 1102 56-57 0.6617814710650691 1102 58-59 0.9318441846530234 1102 60-61 0.7926546916456303 1102 62-63 0.7223627707233575 1102 64-65 0.7540303698451751 1102 66-67 0.9129889340054334 1102 68-69 0.9897687344672406 1102 70-71 0.9081682266213917 1102 72-73 1.101792090304201 1102 74-75 0.9580647847877586 1102 76-77 0.8737798859769583 1102 78-79 0.8422207045296801 1102 80-81 1.2783021316234553 1102 82-83 1.3001175596343515 1102 84-85 1.1410265153118573 1102 86-87 1.2134575418761315 1102 88-89 1.296041001529936 1102 90-91 1.0705857877763556 1102 92-93 1.2864988527251278 1102 94-95 1.4565403430210786 1102 96-97 1.397168944394295 1102 98-99 1.266192640166313 1102 100-101 0.5589703316610688 1103 1 0.23462408879635177 1103 2 0.36088463270075977 1103 3 0.15122511142639894 1103 4 0.10154368783594236 1103 5 0.05656041163449288 1103 6 0.07502976910153336 1103 7 0.01073906872876762 1103 8 -0.2144498631406151 1103 9 0.31701693272695053 1103 10-11 0.18190988907942085 1103 12-13 0.19398972204108134 1103 14-15 0.22710003791336675 1103 16-17 0.3160798764014885 1103 18-19 0.23006538813561406 1103 20-21 0.14252887379649337 1103 22-23 0.061975509390343575 1103 24-25 0.11547374496989704 1103 26-27 0.2308219963524749 1103 28-29 0.18135903777861273 1103 30-31 0.05903774918484572 1103 32-33 0.3661313876012997 1103 34-35 0.3300249338726928 1103 36-37 0.1399039242533746 1103 38-39 -0.06708762600913332 1103 40-41 0.05361841477559892 1103 42-43 0.1252687051773833 1103 44-45 0.5548795747736222 1103 46-47 0.6373276731605202 1103 48-49 0.5922319135235554 1103 50-51 0.2915414470789983 1103 52-53 0.1690487154458964 1103 54-55 0.1605355641624655 1103 56-57 -0.07089719778381465 1103 58-59 -0.1551989140922032 1103 60-61 -0.0743938626369669 1103 62-63 -0.16438027346649875 1103 64-65 0.20569430874315486 1103 66-67 0.049513756700470424 1103 68-69 -0.6199966774258243 1103 70-71 -0.43888578319857174 1103 72-73 -0.3359875059860258 1103 74-75 -0.8827153570562061 1103 76-77 -0.9560619034392985 1103 78-79 -0.7173810412422768 1103 80-81 -0.5132690631392265 1103 82-83 -0.9583112456029639 1103 84-85 -0.9584824863248045 1103 86-87 -0.7345511869836656 1103 88-89 -0.6589508151640047 1103 90-91 -0.583696809059429 1103 92-93 -0.6506806344543534 1103 94-95 -0.5844852980045694 1103 96-97 -0.3744895389663192 1103 98-99 -0.9330708622886839 1103 100-101 -0.8713624561185291 1104 1 0.3835602590091227 1104 2 0.3467002355376394 1104 3 0.06611872844767674 1104 4 0.10863588641750255 1104 5 0.2196809790103771 1104 6 -0.08809079827435085 1104 7 0.15258304035997128 1104 8 0.04086928579555149 1104 9 0.11134317386170522 1104 10-11 -0.034402167658171834 1104 12-13 -0.0435989304411919 1104 14-15 0.1242631584807441 1104 16-17 0.15650540831638438 1104 18-19 -0.03943815796367289 1104 20-21 -0.10215197726733294 1104 22-23 -0.0727762636592999 1104 24-25 -0.026370226661306617 1104 26-27 -0.02804325187447887 1104 28-29 -0.29027216789514654 1104 30-31 -0.1856431018789877 1104 32-33 -0.50975513722139 1104 34-35 -0.4820318037159552 1104 36-37 -0.01967054383173661 1104 38-39 -0.08836422175381387 1104 40-41 -0.0669489611109313 1104 42-43 -0.30735540829779495 1104 44-45 -0.06214170182212797 1104 46-47 0.10541277954349226 1104 48-49 -0.38294539144098394 1104 50-51 -0.30420323377207126 1104 52-53 -0.22456830583069376 1104 54-55 -0.3749254287453354 1104 56-57 -0.20919507012424532 1104 58-59 0.01855995115602127 1104 60-61 -0.031840671147605804 1104 62-63 -0.41969942240266533 1104 64-65 -0.37231987565400715 1104 66-67 -0.33701106599456665 1104 68-69 -0.492337102957741 1104 70-71 -0.35732549951062964 1104 72-73 0.05053731670900419 1104 74-75 -0.04583592443209028 1104 76-77 -0.06599098145348137 1104 78-79 0.052122504857017304 1104 80-81 0.015099731187014243 1104 82-83 -0.22072259312069775 1104 84-85 -0.21380163526097107 1104 86-87 0.08459774918654972 1104 88-89 -0.056113935731382014 1104 90-91 -0.4099379438112045 1104 92-93 -0.5833047479295317 1104 94-95 -0.9887406171535034 1104 96-97 -0.9418654254911409 1104 98-99 -1.1954822098064106 1104 100-101 -1.101858910019235 1105 1 0.36439215473463094 1105 2 0.3295927024726595 1105 3 0.2563621633719677 1105 4 0.2118561279356186 1105 5 0.2641509809271909 1105 6 0.19042175683394902 1105 7 0.24061255924055303 1105 8 0.11131206900429191 1105 9 0.30911008971372667 1105 10-11 0.3291928102985082 1105 12-13 0.10682276785284728 1105 14-15 0.3715556637519768 1105 16-17 0.3220459488569247 1105 18-19 0.3552810293087063 1105 20-21 0.27548363707040124 1105 22-23 0.3166716949375896 1105 24-25 -0.031186212860276896 1105 26-27 7.089045372552505E-4 1105 28-29 -0.20758575808108048 1105 30-31 -0.15281772330892807 1105 32-33 -0.013876279640406608 1105 34-35 0.04612134943719326 1105 36-37 0.018234382371062452 1105 38-39 -0.23025611364570153 1105 40-41 -0.24993247654124673 1105 42-43 0.3412692802205157 1105 44-45 0.43258706950238945 1105 46-47 -0.14211932703116048 1105 48-49 0.5066223678076227 1105 50-51 0.7181276077077143 1105 52-53 0.1658859782406097 1105 54-55 0.501751780378676 1105 56-57 0.5588708681544645 1105 58-59 0.3237880515968854 1105 60-61 0.6547129037038388 1105 62-63 0.4093690077295946 1105 64-65 0.5982091640239631 1105 66-67 0.4535294745459737 1105 68-69 0.6856627053612172 1105 70-71 0.8579083513615231 1105 72-73 1.2605238990360164 1105 74-75 1.0364431631661404 1105 76-77 0.320869283066358 1105 78-79 0.6792789165878901 1105 80-81 0.558395686717013 1105 82-83 0.5713753558921439 1105 84-85 0.5814111306964733 1105 86-87 0.616991845410432 1105 88-89 0.7344505649395003 1105 90-91 0.35831967551024135 1105 92-93 0.3819770232033015 1105 94-95 0.31979398127471725 1105 96-97 0.6112541834795309 1105 98-99 0.4054857794594504 1105 100-101 0.12243187012260748 1106 1 0.31842782305601247 1106 2 0.31152872012295774 1106 3 0.30609557024823175 1106 4 0.12774140096374964 1106 5 0.07827122361886296 1106 6 0.14059642129432603 1106 7 0.1702411674405937 1106 8 0.2377139811204927 1106 9 0.14304095935887773 1106 10-11 0.1904494751266057 1106 12-13 0.1639564321293676 1106 14-15 0.434365922990807 1106 16-17 0.24494078246604545 1106 18-19 0.26863826409451264 1106 20-21 0.06921869866261687 1106 22-23 0.20548397660701312 1106 24-25 0.3071802147918632 1106 26-27 0.11047172858579302 1106 28-29 0.4409479797383753 1106 30-31 0.2940934736022669 1106 32-33 0.7976699604772648 1106 34-35 0.4771518697534276 1106 36-37 0.655897555748524 1106 38-39 0.8810235333482268 1106 40-41 0.979222699042495 1106 42-43 0.5220699788783492 1106 44-45 0.7651849735288678 1106 46-47 1.130380213325516 1106 48-49 1.2822015183867848 1106 50-51 1.191744081324181 1106 52-53 1.3844923397041171 1106 54-55 1.2965899859311705 1106 56-57 1.465287488856795 1106 58-59 1.1794370679601869 1106 60-61 1.3035913740108853 1106 62-63 1.3166589507338244 1106 64-65 1.6372328816191128 1106 66-67 1.6663902945496503 1106 68-69 1.7880942185070126 1106 70-71 1.1967344223304437 1106 72-73 1.197736621963024 1106 74-75 1.061126009277558 1106 76-77 0.9194627745169939 1106 78-79 1.186150584173845 1106 80-81 1.277438709331463 1106 82-83 1.1557428866882518 1106 84-85 0.9768192939617819 1106 86-87 0.9463722462194042 1106 88-89 1.0022157791332873 1106 90-91 1.0883251912300445 1106 92-93 1.1350336512599277 1106 94-95 0.9597323890702576 1106 96-97 0.9771532458071661 1106 98-99 1.2453920122228261 1106 100-101 1.3137584007348515 1107 1 0.24533191802733967 1107 2 -0.04114568297161014 1107 3 0.18738141792063345 1107 4 0.11266870521878047 1107 5 0.14403086080707084 1107 6 -0.05388136982212188 1107 7 0.20612218651488945 1107 8 0.1446100728905293 1107 9 0.14610885318307965 1107 10-11 0.18524739429427228 1107 12-13 0.1852983022107395 1107 14-15 -0.14913414370253975 1107 16-17 -0.0526449184810005 1107 18-19 0.24863026089322915 1107 20-21 0.32191977909478453 1107 22-23 0.21682184508774327 1107 24-25 0.22241297456243814 1107 26-27 0.26099860600342595 1107 28-29 0.31743190664246157 1107 30-31 0.40287031767323356 1107 32-33 0.6528787106439893 1107 34-35 0.34149760804874774 1107 36-37 0.44514658869503876 1107 38-39 0.15019786974945504 1107 40-41 0.09366847735382322 1107 42-43 0.1491874925504817 1107 44-45 0.2563814521203085 1107 46-47 0.28737634511156784 1107 48-49 0.7383172980319728 1107 50-51 0.452715136412877 1107 52-53 0.10591424179828124 1107 54-55 -0.06495463191596684 1107 56-57 0.20778448765631907 1107 58-59 -0.07099643877584327 1107 60-61 0.11271502347067042 1107 62-63 0.31552516388574503 1107 64-65 0.09701679518453687 1107 66-67 0.14664906472438588 1107 68-69 0.4125439984189754 1107 70-71 0.18119487317745353 1107 72-73 0.3170510143866565 1107 74-75 -0.023307764231844885 1107 76-77 -0.25407330658211436 1107 78-79 -0.08575817933154894 1107 80-81 0.11070534932079568 1107 82-83 0.27392349226381185 1107 84-85 0.3354960980167334 1107 86-87 0.31898795917124545 1107 88-89 0.30106865361641866 1107 90-91 0.6292082111046646 1107 92-93 0.4636984505129682 1107 94-95 0.42205064970785244 1107 96-97 0.5103665311212922 1107 98-99 0.7726028965765686 1107 100-101 0.5497194518219537 1108 1 0.29376795911043985 1108 2 0.32428382216377827 1108 3 0.30864659065639444 1108 4 0.23148289756238682 1108 5 0.0884753052515137 1108 6 0.1814127478249361 1108 7 0.23996905859705464 1108 8 0.14332622601845202 1108 9 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-1.35756041747225 2316 48-49 -1.2848980487127903 2316 50-51 -1.6783857888056843 2316 52-53 -1.6618898495352212 2316 54-55 -1.151152747525849 2316 56-57 -1.2384849292013342 2316 58-59 -1.0367039338950974 2316 60-61 -1.5247945258035855 2316 62-63 -1.2674474190868281 2316 64-65 -1.618796802981997 2316 66-67 -0.9712102001937026 2316 68-69 -1.7938476491491357 2316 70-71 -1.5404331719800055 2316 72-73 -1.6850710465589316 2316 74-75 -1.7088183320953547 2316 76-77 -1.5489973368002623 2316 78-79 -1.4304233181143395 2316 80-81 -1.3941753908540626 2316 82-83 -1.3955867360699443 2316 84-85 -1.32497415068881 2316 86-87 -1.5075547791361927 2316 88-89 -1.4603692548803195 2316 90-91 -2.3356080184174495 2316 92-93 -1.516481500255221 2316 94-95 -1.4914117049309752 2316 96-97 -1.5546958574705094 2316 98-99 -1.1040204800468096 2316 100-101 -1.3099274372366985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 3.0 14 15.0 15 54.0 16 178.0 17 406.0 18 660.0 19 895.0 20 1288.0 21 1626.0 22 1883.0 23 1838.0 24 1539.0 25 1450.0 26 1398.0 27 1472.0 28 1638.0 29 1918.0 30 1818.0 31 1837.0 32 2165.0 33 2903.0 34 3925.0 35 6937.0 36 12090.0 37 29972.0 38 59409.0 39 22452.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68569695544263 20.061027313746 14.752393743771933 22.500881987039435 2 15.537232990506327 21.847804588607595 40.458119066455694 22.156843354430382 3 17.273412776898734 26.783771756329116 33.68522547468354 22.257589992088608 4 11.700825751582279 19.871810719936708 38.839992088607595 29.587371439873415 5 11.884394778481013 37.70643789556962 36.70762460443038 13.701542721518987 6 27.552042128164555 39.306640625 18.38038963607595 14.760927610759495 7 23.174198971518987 35.653802412974684 23.464695411392405 17.707303204113924 8 23.633430577531644 35.18529964398734 22.439304786392405 18.741964992088608 9 24.742879746835442 15.70720431170886 22.080819818037973 37.46909612341772 10-11 22.26593403876582 26.93056517009494 30.84917672072785 19.954324070411392 12-13 23.808655557753163 25.719751285601266 30.032078223892405 20.439514932753163 14-15 21.242397646360757 26.888226859177216 28.62904222705696 23.240333267405063 16-17 19.718836530854432 30.18103490901899 29.121031942246834 20.979096617879748 18-19 19.558136372626585 28.04526799841772 32.382627076740505 20.01396855221519 20-21 20.189193532436708 27.16388943829114 32.84216772151899 19.804749307753163 22-23 19.681442840189874 27.931232693829116 32.41167672072785 19.975647745253163 24-25 19.716055181962027 28.055466277689874 32.50129796281646 19.727180577531644 26-27 19.7577754153481 28.226982792721518 32.42280211629747 19.59243967563291 28-29 19.303797468354432 27.59901602056962 32.4815194818038 20.615667029272153 30-31 19.716982298259495 27.430898931962027 32.930552808544306 19.92156596123418 32-33 19.157622132120252 27.834503560126585 32.86627274525317 20.1416015625 34-35 19.484276107594937 28.04866742484177 32.980926127373415 19.486130340189874 36-37 19.61901700949367 28.15157733386076 31.983967068829116 20.245438587816455 38-39 19.526614418512658 27.67318532436709 32.407350178006325 20.392850079113924 40-41 19.700912282436708 27.70007169699367 32.28775217563291 20.311263844936708 42-43 20.073428064601863 27.798826881594156 32.239432354086446 19.888312699717538 44-45 19.80845777294304 28.07957130142405 31.97685917721519 20.13511174841772 46-47 20.03838261471519 27.69080053401899 31.30191851265823 20.968898338607595 48-49 20.175164796449728 27.763373003977364 30.9978027140036 21.0636594855693 50-51 19.96742711275658 28.21757699748441 30.78941350260521 21.025582387153797 52-53 20.00377027294304 28.41147893591772 31.026564972310126 20.558185818829113 54-55 19.733979430379748 28.47205053401899 30.546627768987346 21.247342266613924 56-57 20.234622231012658 28.53911194620253 30.083996736550635 21.14226908623418 58-59 19.658264932753163 28.74431368670886 30.773462223101266 20.823959157436708 60-61 20.123368275316455 28.983509691455694 30.076579806170884 20.81654222705696 62-63 19.469133208069618 29.485079608386076 30.03794996044304 21.007837223101266 64-65 19.38383850870253 29.4501582278481 29.931640625 21.234362638449365 66-67 19.412888152689874 29.30707327927215 29.803389537183545 21.476649030854432 68-69 19.540453674516346 29.182273317263117 29.73391433339514 21.54335867482539 70-71 19.475313983386076 29.194892207278482 29.47086382515823 21.858929984177212 72-73 19.46449762658228 28.84722359572785 29.58119066455696 22.10708811313291 74-75 19.309360166139243 28.693940367879744 29.612712618670884 22.383986847310126 76-77 19.4033679148781 28.51138656851565 29.61743972952844 22.467805787077815 78-79 19.438847409018987 28.54127521756329 29.48446153085443 22.535415842563292 80-81 19.31121439873418 28.69517652294304 29.360846024525316 22.63276305379747 82-83 19.374567345727847 28.438365308544306 29.22455992879747 22.962507416930382 84-85 19.362823872626585 28.28600919699367 29.3701171875 22.981049742879748 86-87 19.362205795094937 28.2890995846519 29.057678995253166 23.291015625 88-89 19.319249406645568 28.04310472705696 29.139883306962027 23.497762559335442 90-91 19.215103342563292 28.03723299050633 28.983200652689874 23.764463014240505 92-93 19.309669204905063 27.48498071598101 29.32901503164557 23.876335047468356 94-95 19.242916831487342 27.33355172072785 29.250828223892405 24.172703223892405 96-97 18.899265723892405 27.424100079113924 29.28667672072785 24.38995747626582 98-99 18.9697265625 27.34189576740506 29.285131526898734 24.403246143196203 100-101 18.852909909018987 27.299866495253166 29.154099090189874 24.693124505537973 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 535.0 1 327.0 2 135.0 3 162.0 4 124.5 5 86.5 6 139.0 7 258.5 8 414.0 9 505.5 10 386.5 11 246.5 12 240.0 13 263.0 14 291.5 15 304.5 16 306.5 17 314.0 18 348.0 19 392.0 20 454.5 21 535.5 22 626.5 23 744.5 24 915.0 25 1101.0 26 1314.5 27 1655.0 28 2139.0 29 2576.5 30 2864.5 31 3275.5 32 3774.0 33 4120.5 34 4251.5 35 4321.0 36 4637.0 37 5060.5 38 5424.5 39 5767.0 40 5972.5 41 6040.0 42 5946.0 43 5931.0 44 5952.0 45 5927.5 46 6000.0 47 5948.5 48 5849.5 49 5790.5 50 5749.5 51 5487.5 52 4897.5 53 4338.0 54 3889.5 55 3343.0 56 2864.5 57 2399.5 58 1915.0 59 1563.0 60 1195.5 61 909.5 62 747.5 63 551.0 64 360.5 65 246.5 66 167.5 67 133.5 68 108.5 69 83.0 70 77.5 71 64.5 72 50.5 73 50.5 74 39.5 75 28.0 76 23.5 77 17.0 78 16.0 79 13.0 80 10.5 81 10.5 82 9.0 83 3.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13906744462025317 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 6.180775316455697E-4 44-45 0.0 46-47 0.0 48-49 9.271162974683545E-4 50-51 6.180775316455697E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0012361550632911393 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.0903876582278483E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 161792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.53628772342795 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14276239784658 52.870785594788515 2 15.380320907890088 21.082201980436192 3 4.314838574649968 8.871690540970677 4 1.6124007537827378 4.420318479469033 5 0.6125369285709437 2.099050358888151 6 0.3505596660337953 1.4415634881312602 7 0.2051542207108631 0.9842356088820746 8 0.11552347913250487 0.6334040323709422 9 0.04282334344678128 0.2641457688972997 >10 0.199178328553464 2.4216773278051216 >50 0.010954808050197034 0.5139580118107919 >100 0.008963024768343028 1.4981910171642607 >500 9.958916409270031E-4 0.3863216928086431 >1k 0.002987674922781009 2.5124560975770587 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1342 0.8294600474683546 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1191 0.7361303401898734 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1148 0.7095530063291139 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.3498318829113924 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 361 0.22312598892405064 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 344 0.21261867088607594 RNA PCR Primer, Index 39 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 337 0.20829212816455694 No Hit ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 239 0.14772053006329114 RNA PCR Primer, Index 39 (96% over 25bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 226 0.13968552215189875 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 0.13165051424050633 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 191 0.11805280854430379 RNA PCR Primer, Index 39 (96% over 30bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.180775316455697E-4 0.0 18-19 0.0 0.0 0.0 6.180775316455697E-4 0.0 20-21 0.0 0.0 0.0 0.0012361550632911393 0.0 22-23 0.0 0.0 0.0 0.004635581487341773 0.0 24-25 0.0 0.0 0.0 0.014524821993670885 0.0 26-27 0.0 0.0 0.0 0.03492138053797468 0.0 28-29 0.0 0.0 0.0 0.07787776898734178 0.0 30-31 0.0 0.0 0.0 0.14617533623417722 0.0 32-33 0.0 0.0 0.0 0.2218898338607595 0.0 34-35 0.0 0.0 0.0 0.33376186708860756 0.0 36-37 0.0 0.0 0.0 0.4783920094936709 0.0 38-39 0.0 0.0 0.0 0.6567073773734178 0.0 40-41 0.0 0.0 0.0 0.8779791337025316 0.0 42-43 0.0 0.0 0.0 1.0547493077531644 0.0 44-45 0.0 0.0 0.0 1.225029667721519 0.0 46-47 0.0 0.0 0.0 1.401799841772152 0.0 48-49 0.0 0.0 0.0 1.5968033030063291 0.0 50-51 0.0 0.0 0.0 1.802314082278481 0.0 52-53 0.0 0.0 0.0 1.9608509691455696 0.0 54-55 0.0 0.0 0.0 2.1620352056962027 0.0 56-57 0.0 0.0 0.0 2.4179193037974684 0.0 58-59 0.0 0.0 0.0 2.685546875 0.0 60-61 0.0 0.0 0.0 2.955337717563291 0.0 62-63 0.0 0.0 0.0 3.2177116297468356 0.0 64-65 0.0 0.0 0.0 3.4878115110759493 0.0 66-67 0.0 0.0 0.0 3.825899920886076 0.0 68-69 0.0 0.0 0.0 4.195819323575949 0.0 70-71 0.0 0.0 0.0 4.619511471518987 0.0 72-73 0.0 0.0 0.0 5.0385680379746836 0.0 74-75 0.0 0.0 0.0 5.493782140031646 0.0 76-77 0.0 0.0 0.0 5.950232397151899 0.0 78-79 0.0 0.0 0.0 6.443767306170886 0.0 80-81 0.0 0.0 0.0 6.981185719936709 0.0 82-83 0.0 0.0 0.0 7.539927808544304 0.0 84-85 0.0 0.0 0.0 8.153987836234176 0.0 86-87 0.0 0.0 0.0 8.829237539556962 0.0 88-89 0.0 0.0 0.0 9.551770174050633 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAGAC 30 1.2979872E-4 63.3321 3 GGTATCA 700 0.0 48.94697 1 GTATCAA 1085 0.0 32.894474 1 CTGGTGT 75 3.0672038E-4 31.666046 4 ATGGGAC 65 0.005829988 29.230198 7 TCAACGC 1210 0.0 28.656466 4 TATCAAC 1255 0.0 28.3859 2 CAACGCA 1225 0.0 28.305569 5 AACGCAG 1230 0.0 28.190506 6 ATCAACG 1235 0.0 28.076376 3 GTTCTAT 70 0.008295928 27.192762 1 TTAGGTT 70 0.008371439 27.142328 4 ACTGACC 70 0.008371439 27.142328 8 CTTTTCG 35 0.008383613 27.142328 46-47 AAACGAC 35 0.008383613 27.142328 46-47 CATGGGG 250 0.0 26.599482 4 ACGCAGA 1370 0.0 25.309725 7 CGCAGAG 1385 0.0 25.035612 8 GAGTACT 960 0.0 24.24432 12-13 GTCTGGC 100 0.0016477183 23.749537 7 >>END_MODULE