FastQCFastQC Report
Fri 10 Feb 2017
SRR2031812_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031812_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659166
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.2876746509993575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.25699658744212456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.16134612208784413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.053.647061
GTATCAA41300.037.493951
ATCAACG49750.030.6482413
TCAACGC50000.030.594
CAACGCA51150.030.0879765
AACGCAG53150.029.1345236
TATCAAC53100.028.8041422
ACGCAGA58450.026.2489327
GTACATG34800.026.0704041
CGCAGAG58650.025.9974428
TACATGG35150.024.0540542
ACATGGG37250.022.3154373
GCAGAGT67000.022.1194049
AGAGTAC59000.021.81779710-11
CACGCCG1100.002877898321.590915
GAGTACT46800.021.21260612-13
CAGAGTA65100.020.97734310-11
AGTACTT49850.019.53360212-13
GTGGTAT10800.018.9120371
GTCTTAG6550.018.8549611
Produced by FastQC (version 0.11.5)