FastQCFastQC Report
Fri 10 Feb 2017
SRR2031812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659166
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57570.34698155579369394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52210.3146761686292993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34440.2075741667801775No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT26510.15977906972539216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.060.4261051
GTATCAA41100.041.9057121
TCAACGC52200.032.4850654
ATCAACG52450.032.4207883
AACGCAG54250.031.520196
CAACGCA53800.031.5189675
TATCAAC56200.030.2574792
ACGCAGA60100.028.2940167
CGCAGAG60500.027.7929048
GCAGAGT69550.023.903259
GTACATG35850.022.6941241
AGAGTAC62150.022.4694410-11
CAGAGTA67200.022.05318610-11
GAGTACT50000.021.89711812-13
TACATGG36350.021.8221782
ACATGGG36400.020.7482643
AGTACTT54450.019.88946212-13
GTACTTT55900.019.6284614-15
CATGGGG23500.018.3932954
ACCGTAT1300.007565265518.2689138
Produced by FastQC (version 0.11.5)