FastQCFastQC Report
Fri 10 Feb 2017
SRR2031811_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031811_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089480
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.3145537320556596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.30592576274920147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20740.19036604618717184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.055.7068021
GTATCAA31400.039.7850341
TCAACGC38750.031.5032254
CAACGCA39500.030.9050645
ATCAACG39550.030.8659923
AACGCAG39650.030.7881476
TATCAAC40600.030.184732
CGCAGAG42950.027.7590248
ACGCAGA43750.027.5771437
GCAGAGT48300.023.995869
GAGTACT34850.022.28479212-13
CAGAGTA46900.022.18016810-11
AGAGTAC43700.021.90217410-11
ACATGGG22350.020.6152133
TACATGG23200.020.4741382
GTACATG24900.020.030121
AGTACTT38450.019.88946712-13
TTGGCCG1200.00476334319.7916684
GTACTTT41850.018.84109914-15
CGCAGAA3151.3395402E-716.5873019
Produced by FastQC (version 0.11.5)