##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031811_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1089480 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.654907845944855 34.0 31.0 34.0 31.0 34.0 2 32.78686345779638 34.0 33.0 34.0 31.0 34.0 3 32.86105206153394 34.0 33.0 34.0 31.0 34.0 4 36.0396776443808 37.0 37.0 37.0 35.0 37.0 5 36.0701123471748 37.0 37.0 37.0 35.0 37.0 6 36.14063222821897 37.0 37.0 37.0 35.0 37.0 7 36.1608060726218 37.0 37.0 37.0 35.0 37.0 8 36.15672798032089 37.0 37.0 37.0 35.0 37.0 9 38.00889506920733 39.0 39.0 39.0 37.0 39.0 10-11 38.04209898300107 39.0 39.0 39.0 37.0 39.0 12-13 38.02183702316701 39.0 39.0 39.0 37.0 39.0 14-15 39.6177515879135 41.0 40.0 41.0 38.0 41.0 16-17 39.55910893270184 41.0 40.0 41.0 38.0 41.0 18-19 39.56295663986489 41.0 40.0 41.0 37.5 41.0 20-21 39.50909608253479 41.0 40.0 41.0 37.5 41.0 22-23 39.42462963982817 41.0 40.0 41.0 37.0 41.0 24-25 39.39271395528142 41.0 40.0 41.0 37.0 41.0 26-27 39.27063690935125 41.0 40.0 41.0 36.5 41.0 28-29 39.14916886955245 41.0 39.0 41.0 36.0 41.0 30-31 38.98956336968095 41.0 39.0 41.0 35.5 41.0 32-33 38.862602801336415 41.0 39.0 41.0 35.0 41.0 34-35 38.6591658405845 41.0 39.0 41.0 35.0 41.0 36-37 38.54136193413372 40.0 38.0 41.0 35.0 41.0 38-39 38.35117670815435 40.0 38.0 41.0 34.5 41.0 40-41 38.26227833461835 40.0 38.0 41.0 34.0 41.0 42-43 38.089407331938176 40.0 38.0 41.0 34.0 41.0 44-45 38.22554062488527 40.0 38.0 41.0 34.0 41.0 46-47 38.09338078716452 40.0 38.0 41.0 33.5 41.0 48-49 37.92086821235819 40.0 38.0 41.0 33.0 41.0 50-51 36.87429324081213 39.0 37.0 40.0 31.5 40.5 52-53 36.89380392480817 39.5 37.5 40.0 32.0 40.5 54-55 37.66682499908213 40.0 38.0 41.0 33.0 41.0 56-57 37.66386946066014 40.5 38.0 41.0 33.0 41.0 58-59 37.545359253956015 40.0 37.0 41.0 33.0 41.0 60-61 37.357009766126964 40.0 37.0 41.0 32.5 41.0 62-63 37.10806439769431 40.0 36.5 41.0 32.0 41.0 64-65 36.802375445166504 39.5 36.0 41.0 31.0 41.0 66-67 36.54255011565151 39.0 35.0 41.0 31.0 41.0 68-69 36.22720472151852 39.0 35.0 41.0 31.0 41.0 70-71 35.86364228806403 37.5 35.0 40.5 30.5 41.0 72-73 35.45746915959907 37.0 35.0 39.5 30.0 41.0 74-75 35.097412986011676 36.5 35.0 39.0 30.0 41.0 76-77 34.71086389837353 36.0 35.0 39.0 29.0 40.5 78-79 34.3253102397474 35.5 35.0 37.5 29.0 39.5 80-81 33.95278710944671 35.0 35.0 37.0 29.0 39.0 82-83 33.6538385284723 35.0 34.5 37.0 29.0 39.0 84-85 33.33463716635459 35.0 34.0 36.0 28.0 37.5 86-87 33.06211357711936 35.0 34.0 36.0 27.0 37.0 88-89 32.84510225061497 35.0 34.0 35.5 27.0 37.0 90-91 32.662777196460695 35.0 34.0 35.0 26.0 36.0 92-93 32.52368607041892 35.0 34.0 35.0 26.0 36.0 94-95 32.36179828909204 35.0 34.0 35.0 25.0 36.0 96-97 32.263034658736274 35.0 34.0 35.0 25.0 36.0 98-99 32.153921136689064 35.0 34.0 35.0 25.0 35.5 100-101 30.680998274406136 34.0 31.5 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13279664869011043 1101 2 0.1601261283104094 1101 3 0.20825825870773684 1101 4 0.09187956648500517 1101 5 0.0971884600339763 1101 6 0.10040836665351804 1101 7 0.12240392641518127 1101 8 0.053442323769353095 1101 9 0.10979203934246584 1101 10-11 0.18864329358198262 1101 12-13 0.18936738535742137 1101 14-15 0.19006170921637278 1101 16-17 0.23679158821197888 1101 18-19 0.24209396904341673 1101 20-21 0.3094547510302803 1101 22-23 0.29192168810695307 1101 24-25 0.215711720445519 1101 26-27 0.08064894636392239 1101 28-29 0.2699272485450095 1101 30-31 0.17713018392529278 1101 32-33 0.11121789076397448 1101 34-35 0.21173698415074682 1101 36-37 0.170308967424873 1101 38-39 0.24020126440421308 1101 40-41 0.1797732034395736 1101 42-43 0.24205719933480907 1101 44-45 0.2673426875343523 1101 46-47 0.48029201006627886 1101 48-49 0.5141326800726205 1101 50-51 0.38386976496363445 1101 52-53 0.4008021625931022 1101 54-55 0.5008294132674607 1101 56-57 0.46163953815899816 1101 58-59 0.4137974566004061 1101 60-61 0.35714457126990595 1101 62-63 0.39970072166291004 1101 64-65 0.5593292544310415 1101 66-67 0.45806686907706506 1101 68-69 0.44747864752797994 1101 70-71 0.6082686053362565 1101 72-73 0.5264744778500585 1101 74-75 0.6693547170630012 1101 76-77 0.5423149591341314 1101 78-79 0.38958025547817243 1101 80-81 0.483809830681615 1101 82-83 0.33576867369666985 1101 84-85 0.29153639518715124 1101 86-87 0.392535157445721 1101 88-89 0.28702514395792633 1101 90-91 0.33583502423157086 1101 92-93 0.30245463949737683 1101 94-95 0.3268079506061241 1101 96-97 0.21955611094016092 1101 98-99 0.3361609401776775 1101 100-101 0.037238601851424136 1102 1 -0.007577981608932305 1102 2 -0.0012277987352646846 1102 3 -0.052221530973497465 1102 4 -0.1292771867190723 1102 5 -0.022712953045221695 1102 6 -0.09514207698762078 1102 7 0.03980780416739549 1102 8 0.10047485745321438 1102 9 0.14238487535956068 1102 10-11 0.22564296495891512 1102 12-13 0.26606472351056 1102 14-15 0.2397330861470337 1102 16-17 0.3611852786490388 1102 18-19 0.21646794209632247 1102 20-21 0.18376299946932306 1102 22-23 0.27763972951346005 1102 24-25 0.31992795442514677 1102 26-27 0.2958214734753213 1102 28-29 0.33506362711879234 1102 30-31 0.26809633574915637 1102 32-33 0.3190916995053499 1102 34-35 0.14725127925427017 1102 36-37 0.11207367330722917 1102 38-39 0.23280724534408392 1102 40-41 0.14063748211193428 1102 42-43 0.11247783686356883 1102 44-45 0.09125987452087969 1102 46-47 0.07419276589934043 1102 48-49 -0.0083057031018825 1102 50-51 -0.0017924105210909147 1102 52-53 0.21504140018758022 1102 54-55 0.29819714248205287 1102 56-57 0.30418636694637513 1102 58-59 0.12308105830923921 1102 60-61 0.20675679604809005 1102 62-63 0.2625728872889397 1102 64-65 0.36440976708303197 1102 66-67 0.20574021774614693 1102 68-69 0.23049540730450957 1102 70-71 0.22745033389360714 1102 72-73 0.28928749132359854 1102 74-75 0.19765902202521346 1102 76-77 0.03745791016929445 1102 78-79 0.056596358008569325 1102 80-81 0.19048416521989964 1102 82-83 0.2681281873345256 1102 84-85 0.2429396156932313 1102 86-87 0.3055913519905715 1102 88-89 0.06874055637987198 1102 90-91 0.14937100845766338 1102 92-93 0.04203400196862361 1102 94-95 0.054987378801982345 1102 96-97 -0.04729568005556928 1102 98-99 0.15778433814021042 1102 100-101 -0.018384138864977473 1103 1 0.12379082271755237 1103 2 0.21032972412573514 1103 3 0.3124466944654216 1103 4 0.23275659461254605 1103 5 0.11908534658412151 1103 6 0.18691716825722438 1103 7 0.22513273678917045 1103 8 0.2594386903671122 1103 9 0.2732563004462705 1103 10-11 0.29247026846139335 1103 12-13 0.2623605570670051 1103 14-15 0.351701125366219 1103 16-17 0.39121306287212576 1103 18-19 0.28680486298565455 1103 20-21 0.28857020551878776 1103 22-23 0.33633940407512597 1103 24-25 0.317104315822327 1103 26-27 0.3725094988916382 1103 28-29 0.2588146757203518 1103 30-31 0.22883788297936292 1103 32-33 0.3834386512726482 1103 34-35 0.41459797519252817 1103 36-37 0.3710726083444982 1103 38-39 0.3423437188300795 1103 40-41 0.13552149636696953 1103 42-43 0.17278387978334564 1103 44-45 0.2283324764211585 1103 46-47 0.3729713309959308 1103 48-49 0.37712334598754893 1103 50-51 0.3343508775364512 1103 52-53 0.46614890192781644 1103 54-55 0.5621079949876915 1103 56-57 0.47053949430758735 1103 58-59 0.6162938545205918 1103 60-61 0.7246362708251297 1103 62-63 0.611216100977579 1103 64-65 0.5620079489120258 1103 66-67 0.5229212197630275 1103 68-69 0.595359371702628 1103 70-71 0.49687162481880875 1103 72-73 0.4359614163954433 1103 74-75 0.4180253930011091 1103 76-77 0.4529908972410013 1103 78-79 0.29771343846382337 1103 80-81 0.17597984884862683 1103 82-83 0.17652354433501927 1103 84-85 0.26191915531995136 1103 86-87 0.25554524326918227 1103 88-89 0.3849553575769207 1103 90-91 0.5374255773857399 1103 92-93 0.34317630835661106 1103 94-95 0.41435101895166326 1103 96-97 0.4425574264823524 1103 98-99 0.34336509979679164 1103 100-101 0.44534108885261503 1104 1 0.22502655626188073 1104 2 0.22871889319992533 1104 3 0.24864755906887837 1104 4 0.2884382981982938 1104 5 0.32663450275907735 1104 6 0.26102904364439894 1104 7 0.25419923767183406 1104 8 0.34178657301951887 1104 9 0.43253305666510755 1104 10-11 0.31370358616936045 1104 12-13 0.367815692880221 1104 14-15 0.34790892718784505 1104 16-17 0.3503050851820362 1104 18-19 0.3401095165735555 1104 20-21 0.4333685662962452 1104 22-23 0.4170034044563451 1104 24-25 0.4739580324979187 1104 26-27 0.5044740060443971 1104 28-29 0.5042658664063069 1104 30-31 0.4920343891037291 1104 32-33 0.5281267479592913 1104 34-35 0.562764100512382 1104 36-37 0.5067667014029595 1104 38-39 0.6654629403545158 1104 40-41 0.5522338755335952 1104 42-43 0.5096589622043695 1104 44-45 0.514586367524565 1104 46-47 0.565170506730297 1104 48-49 0.5901613267029688 1104 50-51 0.515593459644414 1104 52-53 0.6465553608547125 1104 54-55 0.6272485844739251 1104 56-57 0.7066924854565357 1104 58-59 0.6578783772100607 1104 60-61 0.6767705735959169 1104 62-63 0.629705006415314 1104 64-65 0.6839384238011803 1104 66-67 0.4736896884441464 1104 68-69 0.5178636631367155 1104 70-71 0.4412691562329982 1104 72-73 0.5596727394647942 1104 74-75 0.5711096292255817 1104 76-77 0.6131896607317273 1104 78-79 0.6540088531399206 1104 80-81 0.6497148315997734 1104 82-83 0.47153595043045726 1104 84-85 0.43699027288807457 1104 86-87 0.31704822594054605 1104 88-89 0.37481604804067814 1104 90-91 0.3981481784214509 1104 92-93 0.38429096195559964 1104 94-95 0.3189454299474903 1104 96-97 0.32831108431348355 1104 98-99 0.26953304031845704 1104 100-101 0.27834529218624837 1105 1 0.03609193695466928 1105 2 -0.03244045055993894 1105 3 0.09659305531294393 1105 4 0.13676689455056135 1105 5 0.0687014831536672 1105 6 0.14877242869624752 1105 7 0.19209663932123533 1105 8 0.176263511946118 1105 9 0.20877360211683538 1105 10-11 0.12104305945847926 1105 12-13 0.1126363347282151 1105 14-15 0.2527629090993102 1105 16-17 0.2972452024935066 1105 18-19 0.3526529389000146 1105 20-21 0.35867336384854553 1105 22-23 0.297089964372681 1105 24-25 0.25810855977827174 1105 26-27 0.23813504528110485 1105 28-29 0.32113883765827467 1105 30-31 0.38584835776199355 1105 32-33 0.17611107191703468 1105 34-35 0.26451134762748296 1105 36-37 0.3260040215659359 1105 38-39 0.4145354757006814 1105 40-41 0.2841600925235639 1105 42-43 0.3928941908478336 1105 44-45 0.3634269719944072 1105 46-47 0.2899964291473509 1105 48-49 0.2753208568358616 1105 50-51 0.1000447722799862 1105 52-53 -0.00886421141918703 1105 54-55 0.09623766612084239 1105 56-57 0.1255932987677184 1105 58-59 0.20623234100192178 1105 60-61 0.21717676325118163 1105 62-63 0.15430417498453863 1105 64-65 0.09685925150449748 1105 66-67 0.1433843990741437 1105 68-69 0.17454332414489215 1105 70-71 0.1821433492636757 1105 72-73 0.2629860376978712 1105 74-75 0.20841529651865898 1105 76-77 0.24438695211042472 1105 78-79 0.26557381708191485 1105 80-81 0.2548633864322696 1105 82-83 0.30191734211926047 1105 84-85 0.3143868488014263 1105 86-87 0.40759807813638105 1105 88-89 0.35039652236003604 1105 90-91 0.30554822294389794 1105 92-93 0.35863257335748955 1105 94-95 0.40185916512177755 1105 96-97 0.4133313523800197 1105 98-99 0.34151651524352644 1105 100-101 0.3807153358285973 1106 1 0.3137914160985389 1106 2 0.29512699082550853 1106 3 0.3051543543894084 1106 4 0.3698244805209967 1106 5 0.3689830664395828 1106 6 0.3954218793899926 1106 7 0.3369413308197551 1106 8 0.3027804871872135 1106 9 0.29136066679345163 1106 10-11 0.28278566607350086 1106 12-13 0.26839274330118457 1106 14-15 0.28869031021689295 1106 16-17 0.3533007021214658 1106 18-19 0.28729206001000307 1106 20-21 0.3804880441129157 1106 22-23 0.3498700522033218 1106 24-25 0.35668751252188 1106 26-27 0.4575847177399197 1106 28-29 0.36864325107504925 1106 30-31 0.4231982651167172 1106 32-33 0.4008918600584366 1106 34-35 0.4185330280815194 1106 36-37 0.4619270457412412 1106 38-39 0.2959772404838006 1106 40-41 0.3873961118404665 1106 42-43 0.23902862133469682 1106 44-45 0.22631341952216388 1106 46-47 0.26296005675709466 1106 48-49 0.0793928721259789 1106 50-51 -1.0987968015285787E-4 1106 52-53 0.1462400327558271 1106 54-55 0.17027510356704312 1106 56-57 0.2023094248820101 1106 58-59 0.16005414109238103 1106 60-61 0.28431322172129114 1106 62-63 0.1945049307365707 1106 64-65 0.29015669391123566 1106 66-67 0.25191943649699766 1106 68-69 0.22022547059737718 1106 70-71 0.2677125706056458 1106 72-73 0.3782644841751619 1106 74-75 0.46328318581119987 1106 76-77 0.3012040396930402 1106 78-79 0.33324134454722554 1106 80-81 0.4925080078997155 1106 82-83 0.2225371171444266 1106 84-85 0.2971737938416297 1106 86-87 0.31454136755439777 1106 88-89 0.25168042542535574 1106 90-91 0.18276332047675226 1106 92-93 0.15966699071352508 1106 94-95 0.14813219878045913 1106 96-97 0.2631642990834564 1106 98-99 0.266584722593862 1106 100-101 0.1699670245988223 1107 1 0.14812914142625289 1107 2 0.13111456096213203 1107 3 0.128609116665082 1107 4 0.0971281822407164 1107 5 0.09701193462418445 1107 6 0.09895998114834015 1107 7 0.08419439842877097 1107 8 0.06178835742979061 1107 9 0.0642715086698118 1107 10-11 0.11324885248774308 1107 12-13 0.09451677409708026 1107 14-15 0.04795053110113656 1107 16-17 0.0772723732566547 1107 18-19 0.16616830707312857 1107 20-21 0.22677507338103453 1107 22-23 0.2067717511517415 1107 24-25 0.21775163053817437 1107 26-27 0.15259072587162592 1107 28-29 0.2819753818710353 1107 30-31 0.17668448467635045 1107 32-33 0.11473853107974463 1107 34-35 0.08150234789177091 1107 36-37 0.11011654376388691 1107 38-39 0.30609677451879946 1107 40-41 0.12559689731600798 1107 42-43 0.15671812801196694 1107 44-45 0.13187643020197015 1107 46-47 0.10563491889803345 1107 48-49 0.14518032000464132 1107 50-51 0.2103710532701939 1107 52-53 0.11465611796835873 1107 54-55 0.13244664916461346 1107 56-57 -0.008263975902735865 1107 58-59 0.06160459436696897 1107 60-61 0.11395011921135989 1107 62-63 0.11731159825959026 1107 64-65 -0.012825780928864106 1107 66-67 -0.05687803525932367 1107 68-69 0.07424472394922788 1107 70-71 0.07841223781255025 1107 72-73 0.12408316158449395 1107 74-75 0.12586532503405579 1107 76-77 0.16431156019766746 1107 78-79 0.14442977030740423 1107 80-81 0.08201003900352788 1107 82-83 0.150213605443831 1107 84-85 0.1395184137168357 1107 86-87 0.1790386386145144 1107 88-89 0.14121734833268107 1107 90-91 0.22139118891552556 1107 92-93 0.1653612783877847 1107 94-95 0.37864995488220643 1107 96-97 0.27124680772213594 1107 98-99 0.267085779824626 1107 100-101 0.29924616722612996 1108 1 0.14480512775741516 1108 2 0.03415898469621226 1108 3 0.09216922850105647 1108 4 0.12424840591266673 1108 5 0.07104113623959307 1108 6 0.11533152510608602 1108 7 0.16434351306065054 1108 8 0.08135965286315638 1108 9 0.07694625237903807 1108 10-11 0.0718229438204574 1108 12-13 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-0.4181633439383674 2316 68-69 -0.3610263358607355 2316 70-71 -0.2543403452207116 2316 72-73 -0.26573580161056753 2316 74-75 -0.8069067642737195 2316 76-77 -0.9010932878856082 2316 78-79 -0.48068161671256604 2316 80-81 -0.41113056408094195 2316 82-83 -0.42281448049524073 2316 84-85 -0.32963225832076404 2316 86-87 -0.12926081675792034 2316 88-89 -0.23174757443175764 2316 90-91 -0.24389724076745267 2316 92-93 -0.5200272835043833 2316 94-95 -0.5153554263725795 2316 96-97 -0.4626541685204586 2316 98-99 -0.377147639067978 2316 100-101 -0.12241353807431565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 14.0 7 375.0 8 949.0 9 796.0 10 810.0 11 943.0 12 1162.0 13 1313.0 14 1299.0 15 1314.0 16 1709.0 17 2348.0 18 3009.0 19 4113.0 20 5682.0 21 6964.0 22 8146.0 23 7945.0 24 7186.0 25 6237.0 26 5837.0 27 6053.0 28 6683.0 29 7772.0 30 9252.0 31 11382.0 32 14657.0 33 20199.0 34 29623.0 35 49155.0 36 94291.0 37 222123.0 38 395413.0 39 154468.0 40 257.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81042332121746 19.74859566031501 13.820813599148218 23.62016741931931 2 14.993391342658882 21.524121599295075 40.29592098983001 23.18656606821603 3 16.895583214010355 26.53568674964203 32.09696368909939 24.471766347248227 4 11.759830377794911 18.602452546168816 39.96346881080883 29.674248265227448 5 12.44823218416125 38.40327495686016 36.44637808862944 12.702114770349157 6 29.176120718140762 39.20026067481734 18.675423137643644 12.948195469398243 7 24.984120864999817 33.92389029628814 24.037706061607373 17.054282777104675 8 24.54060652788486 35.37357271358814 22.03638433013915 18.049436428387857 9 25.69390902081727 15.076550280867936 21.447112383889564 37.78242831442523 10-11 23.005608180049197 26.49888019972831 31.04637074567684 19.44914087454565 12-13 24.26272166538165 25.041487682197012 29.950113815765317 20.745676836656017 14-15 21.669557954253406 26.504112053456698 27.893674046334034 23.93265594595587 16-17 20.936180563204466 29.101681536145684 28.476475015603775 21.48566288504608 18-19 20.625711348533248 28.420576788926827 30.329331424165655 20.62438043837427 20-21 21.71054080845908 27.803585196607557 30.161866211403606 20.32400778352976 22-23 21.296555509560076 27.87603065318377 29.79387395259569 21.033539884660467 24-25 21.216674407376743 27.83635832940179 29.92405537330722 21.022911889914255 26-27 21.29336931380108 28.00290046627749 29.777233175459855 20.926497044461577 28-29 21.158672027022067 27.969398245034327 29.932169475346036 20.93976025259757 30-31 21.440136578918384 27.842594632301648 29.90284355839483 20.81442523038514 32-33 21.18189411462349 28.020753019789257 29.760619745199545 21.03673312038771 34-35 21.488141131548996 28.10060762932775 29.634734001542018 20.77651723758123 36-37 21.48924257443918 27.982431985901528 29.507609134633032 21.02071630502625 38-39 21.35881337885964 28.039339868561147 29.642535888680836 20.959310863898374 40-41 21.53100561735874 27.95182105224511 29.545425340529423 20.971747989866728 42-43 21.42549656716966 28.0313544076073 29.400493813562434 21.142655211660607 44-45 21.48235855637552 28.24631934500863 29.15023681022139 21.121085288394465 46-47 21.543855784410912 28.140489040643242 28.961247567646954 21.354407607298896 48-49 21.519440467011787 28.191017733230535 28.954547123398317 21.334994676359365 50-51 21.563406395711716 28.329202922495135 28.713147556632524 21.394243125160628 52-53 21.612695965047546 28.309331057018028 28.617551492455117 21.460421485479312 54-55 21.59277820611668 28.34049271211954 28.62952968388589 21.437199397877887 56-57 21.596541469324816 28.480743106803246 28.48023827881191 21.442477145060028 58-59 21.566940191651064 28.261785438924992 28.704749054594853 21.466525314829095 60-61 21.59502698535081 28.26325402944524 28.656836288871755 21.484882696332193 62-63 21.534860667474394 28.16187539009436 28.74669567132944 21.55656827110181 64-65 21.505718324338215 28.165822227117527 28.66248118368396 21.6659782648603 66-67 21.382540294452397 28.315710247090355 28.55449388699196 21.747255571465285 68-69 21.474051841245366 28.28840364210449 28.517870910893272 21.719673605756874 70-71 21.448489187502297 27.984497191320628 28.60158057054742 21.965433050629656 72-73 21.47354701325403 28.001156515034697 28.555595329882145 21.96970114182913 74-75 21.38111759738591 28.075458016668502 28.519247714506 22.024176671439584 76-77 21.37781326871535 28.04259830377795 28.517228402540663 22.062360024966036 78-79 21.42646032969857 27.94360612402247 28.509839556485662 22.120093989793297 80-81 21.45904468186658 28.133100194588245 28.332644931526968 22.07521019201821 82-83 21.481257113485334 28.02814186584426 28.372985277380035 22.11761574329038 84-85 21.445184858831738 27.947690641406908 28.41667584535742 22.190448654403934 86-87 21.372168373903147 27.974630098762717 28.357381503102395 22.295820024231745 88-89 21.531877592980138 27.834655064801556 28.340355031758268 22.293112310460035 90-91 21.51971582773433 27.82350295553842 28.392031060689504 22.264750156037742 92-93 21.521735139699675 27.638919484524727 28.462569299115177 22.376776076660427 94-95 21.39006682086867 27.645987076403422 28.46160553658626 22.502340566141648 96-97 21.417052171678232 27.52923413004369 28.544948048610348 22.50876564966773 98-99 21.44188053016118 27.52257957924882 28.379089106729815 22.65645078386019 100-101 21.661801960568344 27.352268972353784 28.43076513566105 22.55516393141682 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 444.0 1 297.5 2 206.5 3 308.0 4 273.0 5 227.5 6 331.5 7 604.0 8 953.0 9 1162.0 10 1081.0 11 922.0 12 949.0 13 1040.5 14 1130.0 15 1217.5 16 1299.0 17 1393.0 18 1555.0 19 1790.0 20 2072.0 21 2428.5 22 3059.0 23 3882.0 24 4879.0 25 6120.5 26 7720.0 27 9800.5 28 12136.0 29 14677.0 30 17199.5 31 19496.0 32 21764.0 33 24007.0 34 26216.5 35 28306.5 36 30715.0 37 33333.5 38 35354.0 39 36918.0 40 38149.5 41 39185.5 42 39838.5 43 40388.5 44 41260.5 45 41967.5 46 42156.0 47 42078.0 48 41979.0 49 41091.5 50 39323.0 51 37179.5 52 34660.0 53 32097.5 54 29330.0 55 26224.5 56 23117.0 57 19790.5 58 16854.0 59 14311.0 60 11835.5 61 9642.0 62 7551.0 63 5664.5 64 4140.5 65 3076.5 66 2290.0 67 1706.5 68 1293.5 69 991.5 70 792.0 71 615.0 72 456.0 73 350.5 74 259.5 75 188.0 76 150.5 77 106.0 78 63.5 79 54.5 80 47.5 81 35.5 82 26.0 83 23.5 84 19.0 85 16.0 86 14.5 87 9.0 88 5.5 89 4.5 90 5.0 91 4.5 92 3.0 93 3.0 94 2.5 95 1.0 96 0.5 97 1.0 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.178690751551199E-5 24-25 1.8357381503102398E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1089480.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.07716646389409 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17965050987287 53.38454049941635 2 15.557502039177093 21.804513203235697 3 4.675858510627982 9.83012745632679 4 1.8115833160078885 5.078025023963923 5 0.790694649213765 2.770482027753168 6 0.39482714241671063 1.660102043015967 7 0.21094823878660077 1.0347858391299734 8 0.11614917622652607 0.6511524125656349 9 0.06934914273327344 0.4373812277503159 >10 0.18632878737158587 1.891913799992555 >50 0.003031506634154126 0.13629386281401154 >100 0.003682435867039543 0.5065234911069568 >500 0.0 0.0 >1k 3.945450645699452E-4 0.8141591129286622 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3427 0.3145537320556596 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.30592576274920147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2074 0.19036604618717184 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.5893453757755995E-5 0.0 16-17 0.0 0.0 0.0 1.3768036127326797E-4 0.0 18-19 0.0 0.0 0.0 1.8357381503102398E-4 0.0 20-21 0.0 0.0 0.0 2.2946726878877996E-4 0.0 22-23 0.0 0.0 0.0 4.130410838198039E-4 0.0 24-25 0.0 0.0 0.0 0.0016521643352792157 0.0 26-27 0.0 0.0 0.0 0.0037632632081359913 0.0 28-29 0.0 0.0 0.0 0.009912986011675295 0.0 30-31 0.0 0.0 0.0 0.02111098872856776 0.0 32-33 0.0 0.0 0.0 0.03721959099754011 0.0 34-35 0.0 0.0 0.0 0.06158901494290854 0.0 36-37 0.0 0.0 0.0 0.10248008224106914 0.0 38-39 0.0 0.0 0.0 0.16090244887469252 0.0 40-41 0.0 0.0 0.0 0.23189962183794105 0.0 42-43 0.0 0.0 0.0 0.2896335866651981 0.0 44-45 0.0 0.0 0.0 0.36274185850130336 0.0 46-47 0.0 0.0 0.0 0.4530601754965672 0.0 48-49 0.0 0.0 0.0 0.5546223886624811 0.0 50-51 0.0 0.0 0.0 0.6555420934757866 0.0 52-53 0.0 0.0 0.0 0.7518724529133165 0.0 54-55 0.0 0.0 0.0 0.8639901604435143 0.0 56-57 0.0 0.0 0.0 1.0048830634798254 0.0 58-59 0.0 0.0 0.0 1.1650971105481513 0.0 60-61 0.0 0.0 0.0 1.3335719792928737 0.0 62-63 0.0 0.0 0.0 1.5025057825751735 0.0 64-65 0.0 0.0 0.0 1.6802511289789623 0.0 66-67 0.0 0.0 0.0 1.887230605426442 0.0 68-69 0.0 0.0 0.0 2.1470334471490986 0.0 70-71 0.0 0.0 0.0 2.4194110952013803 0.0 72-73 0.0 0.0 0.0 2.696561662444469 1.8357381503102398E-4 74-75 0.0 0.0 0.0 2.97917355068473 1.8357381503102398E-4 76-77 0.0 0.0 0.0 3.2836307229136836 1.8357381503102398E-4 78-79 0.0 0.0 0.0 3.645638286154863 1.8357381503102398E-4 80-81 0.0 0.0 0.0 4.041010390277931 1.8357381503102398E-4 82-83 0.0 0.0 0.0 4.4554282777104675 1.8357381503102398E-4 84-85 0.0 0.0 0.0 4.892563424753094 1.8357381503102398E-4 86-87 0.0 0.0 0.0 5.368019605683445 1.8357381503102398E-4 88-89 0.0 0.0 0.0 5.906579285530713 1.8357381503102398E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1910 0.0 55.706802 1 GTATCAA 3140 0.0 39.785034 1 TCAACGC 3875 0.0 31.503225 4 CAACGCA 3950 0.0 30.905064 5 ATCAACG 3955 0.0 30.865992 3 AACGCAG 3965 0.0 30.788147 6 TATCAAC 4060 0.0 30.18473 2 CGCAGAG 4295 0.0 27.759024 8 ACGCAGA 4375 0.0 27.577143 7 GCAGAGT 4830 0.0 23.99586 9 GAGTACT 3485 0.0 22.284792 12-13 CAGAGTA 4690 0.0 22.180168 10-11 AGAGTAC 4370 0.0 21.902174 10-11 ACATGGG 2235 0.0 20.615213 3 TACATGG 2320 0.0 20.474138 2 GTACATG 2490 0.0 20.03012 1 AGTACTT 3845 0.0 19.889467 12-13 TTGGCCG 120 0.004763343 19.791668 4 GTACTTT 4185 0.0 18.841099 14-15 CGCAGAA 315 1.3395402E-7 16.587301 9 >>END_MODULE