FastQCFastQC Report
Fri 10 Feb 2017
SRR2031811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089480
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41580.38164996144949886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37970.348514887836399No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT30380.27884862503212543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.21992143040716672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.059.825871
GTATCAA33850.040.6054081
CAACGCA40500.033.6599245
TCAACGC40500.033.6599244
ATCAACG40950.033.174043
AACGCAG41750.032.7659156
TATCAAC42800.031.7401182
CGCAGAG46150.029.5390458
ACGCAGA46450.029.4505257
GCAGAGT52100.025.6185689
CAGAGTA50000.023.51210210-11
GAGTACT41400.023.23330112-13
AGAGTAC47250.023.12130210-11
GTACATG23200.022.9601521
TACATGG23450.022.483632
ACATGGG22700.021.7617473
GTACTTT45500.020.8265714-15
AGTACTT44400.020.80764412-13
TACTTTT53100.017.3090314-15
CATGGGG14400.017.1524894
Produced by FastQC (version 0.11.5)