FastQCFastQC Report
Fri 10 Feb 2017
SRR2031810_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031810_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431623
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.26875305532837684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11510.26666790231289805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.14433892540480928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.064.105691
GTATCAA11050.037.8280531
AACGCAG12750.031.6666666
CAACGCA12800.031.542975
TCAACGC12900.031.2984494
ATCAACG12950.030.8108083
TATCAAC13600.030.7352922
CGTCCGA350.00839043327.14285910-11
ACGCAGA15200.026.56257
CGCAGAG15350.026.3029338
CTACTAT2007.1486284E-1026.1251
CTATACA3700.025.6756744
GTACATG10450.024.5454541
GTGTTAG1801.9451545E-723.751
GCAGAGT18100.022.5690619
GTACCTG1954.2088323E-721.9230771
GTATATA2858.367351E-1121.6666681
GTATAAT2858.367351E-1121.6666681
ATATAGG1553.8991537E-521.4516122
CAGAGTA17350.021.35446710-11
Produced by FastQC (version 0.11.5)