FastQCFastQC Report
Fri 10 Feb 2017
SRR2031810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431623
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.34242846187529397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.335477951823698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.17190928194280658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7850.058.195411
GTATCAA14600.034.5493771
TCAACGC15200.032.499094
ATCAACG15250.032.3925323
CAACGCA15800.031.2649465
TATCAAC16050.031.0738952
AACGCAG16550.030.7091086
CGCAGAG17800.028.2857258
ACGCAGA17850.028.2064917
GTACTAT1257.2729217E-626.6486471
GTATAGG1858.762072E-925.7226351
TATAGGG1151.280185E-424.7819162
GTACATG11250.024.1106821
GCAGAGT20350.024.0410949
GTGGTAT2801.8189894E-1223.7934361
GAGTACT13800.023.74933412-13
CAGAGTA20200.022.92633610-11
TTATATC2354.629328E-922.233423
GCGAGAG652.9412445E-421.92246236-37
CTAGACA1754.040541E-621.7136764
Produced by FastQC (version 0.11.5)