##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031810_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 431623 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92556467102077 34.0 33.0 34.0 31.0 34.0 2 33.05961915838591 34.0 33.0 34.0 31.0 34.0 3 33.114071770966795 34.0 33.0 34.0 31.0 34.0 4 36.46251010720003 37.0 37.0 37.0 35.0 37.0 5 36.457779126691584 37.0 37.0 37.0 35.0 37.0 6 36.51538958767257 37.0 37.0 37.0 35.0 37.0 7 36.46443308164764 37.0 37.0 37.0 35.0 37.0 8 36.509296307194006 37.0 37.0 37.0 35.0 37.0 9 38.32167655569791 39.0 39.0 39.0 37.0 39.0 10-11 38.32909970043302 39.0 39.0 39.0 37.0 39.0 12-13 38.35324345551558 39.0 39.0 39.0 37.0 39.0 14-15 39.93650824909702 41.0 40.0 41.0 38.0 41.0 16-17 39.92163763284162 41.0 40.0 41.0 38.0 41.0 18-19 39.95516689333053 41.0 40.0 41.0 38.0 41.0 20-21 39.957420017005575 41.0 40.0 41.0 38.0 41.0 22-23 39.917577376553155 41.0 40.0 41.0 38.0 41.0 24-25 39.85930545869891 41.0 40.0 41.0 38.0 41.0 26-27 39.749925282016946 41.0 40.0 41.0 37.0 41.0 28-29 39.714072234334125 41.0 40.0 41.0 37.0 41.0 30-31 39.59021646205137 41.0 40.0 41.0 37.0 41.0 32-33 39.1556983756658 40.5 39.0 41.0 35.5 41.0 34-35 39.20537367100456 41.0 39.0 41.0 35.5 41.0 36-37 39.15790632102552 41.0 39.0 41.0 35.0 41.0 38-39 39.0878938332758 41.0 39.0 41.0 35.0 41.0 40-41 38.99900376022594 41.0 39.0 41.0 35.0 41.0 42-43 38.87141556404548 40.5 39.0 41.0 35.0 41.0 44-45 38.66856840344467 40.0 38.5 41.0 35.0 41.0 46-47 38.48033353180901 40.0 38.0 41.0 35.0 41.0 48-49 38.34885536683633 40.0 38.5 41.0 34.5 41.0 50-51 38.38978344527516 40.0 39.0 41.0 35.0 41.0 52-53 38.47560602655558 41.0 39.0 41.0 35.0 41.0 54-55 38.41544472838565 41.0 39.0 41.0 35.0 41.0 56-57 38.28746266996893 41.0 38.0 41.0 34.5 41.0 58-59 38.14198038566063 40.0 38.0 41.0 34.0 41.0 60-61 37.955693973676105 40.0 37.0 41.0 34.0 41.0 62-63 37.7228646295494 40.0 37.0 41.0 34.0 41.0 64-65 37.46646958109276 39.5 36.0 41.0 34.0 41.0 66-67 37.21903142325594 39.0 36.0 41.0 33.0 41.0 68-69 36.87253343774544 39.0 35.0 41.0 33.0 41.0 70-71 36.525006776747304 37.5 35.0 40.5 33.0 41.0 72-73 36.14101310634512 37.0 35.0 39.5 32.0 41.0 74-75 35.7190800768263 37.0 35.0 39.0 32.0 41.0 76-77 34.241268421747684 35.5 33.5 38.0 29.5 39.0 78-79 34.92104568106889 36.0 35.0 37.5 31.0 39.0 80-81 34.732690333925675 35.0 35.0 37.0 31.5 39.0 82-83 34.420911304541235 35.0 35.0 37.0 31.0 39.0 84-85 34.14767169497455 35.0 35.0 36.0 31.0 38.0 86-87 33.88499686068629 35.0 35.0 36.0 31.0 37.0 88-89 33.699598955570025 35.0 35.0 36.0 31.0 37.0 90-91 33.49700201333108 35.0 34.5 35.0 31.0 36.5 92-93 33.35500772665034 35.0 34.0 35.0 30.0 36.0 94-95 33.27441308734706 35.0 34.0 35.0 30.0 36.0 96-97 33.18461713115381 35.0 34.0 35.0 30.0 36.0 98-99 33.12362640545105 35.0 34.0 35.0 30.0 36.0 100-101 31.998367788556216 34.5 32.0 35.0 26.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2026737584464371 1101 2 0.19907226778960307 1101 3 0.19951718171044774 1101 4 0.11162922048000468 1101 5 0.14807693232183539 1101 6 0.06181713463895733 1101 7 0.2464074853031093 1101 8 0.11730061406743886 1101 9 0.1701842256108108 1101 10-11 0.08760737624292858 1101 12-13 0.024762645281768414 1101 14-15 0.03743776324314041 1101 16-17 0.21153962479568378 1101 18-19 0.16176178566674793 1101 20-21 0.29786539369191445 1101 22-23 0.2207711434656474 1101 24-25 0.16356750918896523 1101 26-27 0.3526212444052703 1101 28-29 0.19116810209984436 1101 30-31 0.2278976606663079 1101 32-33 0.19762418272101456 1101 34-35 0.248568428864111 1101 36-37 0.2833672335741184 1101 38-39 0.4759300675720155 1101 40-41 0.5167017965734573 1101 42-43 0.3963105981322599 1101 44-45 0.6728635165064034 1101 46-47 0.6175962843830121 1101 48-49 0.5298497123494528 1101 50-51 0.6710172470991722 1101 52-53 0.530746116838948 1101 54-55 0.29633001044363283 1101 56-57 0.3182939522706647 1101 58-59 0.24972618289200454 1101 60-61 0.37269362860137534 1101 62-63 0.3019728480037358 1101 64-65 0.26364254829429257 1101 66-67 0.18407464463076195 1101 68-69 0.08732111321457126 1101 70-71 0.17177402503016737 1101 72-73 0.10262106594873899 1101 74-75 0.028727901705977388 1101 76-77 -0.024044509785234425 1101 78-79 -0.0471913171071634 1101 80-81 0.02273082003079452 1101 82-83 -0.1874978620755101 1101 84-85 0.05970446750350078 1101 86-87 0.31139247952037863 1101 88-89 0.09659537757639924 1101 90-91 0.16293449101635105 1101 92-93 0.13783303034170302 1101 94-95 0.02976475192336636 1101 96-97 0.2532006758844787 1101 98-99 0.26060876222845764 1101 100-101 -0.24157154214972465 1102 1 0.1978611241132029 1102 2 0.21432785137992028 1102 3 0.3203937353035684 1102 4 0.22559053250691363 1102 5 0.17893853404130056 1102 6 0.09415056142150036 1102 7 0.23278726289399287 1102 8 0.07335612289838878 1102 9 0.25685836821430996 1102 10-11 0.15665466154336372 1102 12-13 0.11124988946455261 1102 14-15 0.053732969298650346 1102 16-17 0.22537010792810008 1102 18-19 0.16811633197082898 1102 20-21 0.22972923819242652 1102 22-23 0.14747191909409452 1102 24-25 0.05415016501552117 1102 26-27 0.2916689969517634 1102 28-29 0.15065786941131165 1102 30-31 0.27860553843473923 1102 32-33 0.26574865706921 1102 34-35 0.12467813823706564 1102 36-37 0.27809903434039995 1102 38-39 0.24153608570116347 1102 40-41 0.2988016003301226 1102 42-43 0.2734015242139307 1102 44-45 0.08405172321605647 1102 46-47 0.12666085778735692 1102 48-49 0.06998614819656979 1102 50-51 0.0999608037761135 1102 52-53 0.0950975793190949 1102 54-55 0.11535393344147593 1102 56-57 -0.12841663552486438 1102 58-59 0.16807493534504658 1102 60-61 0.18373932526523618 1102 62-63 -0.0333579622009168 1102 64-65 0.15854723009972815 1102 66-67 0.4326378630215757 1102 68-69 0.4870851539584322 1102 70-71 0.4915338605595494 1102 72-73 0.34914008286304465 1102 74-75 0.343406436422363 1102 76-77 0.25342955805889034 1102 78-79 0.47496782689806594 1102 80-81 0.35035207038122707 1102 82-83 0.281243377061017 1102 84-85 0.35257429275348073 1102 86-87 0.4510172809408104 1102 88-89 0.3179181511489588 1102 90-91 0.23053331355630036 1102 92-93 0.46682097539356704 1102 94-95 0.21819340345125227 1102 96-97 0.3076815655209657 1102 98-99 0.12183668485251076 1102 100-101 0.14704868025939177 1103 1 0.37048985040046034 1103 2 0.3271089251366064 1103 3 0.324649210290751 1103 4 0.23996100053592073 1103 5 0.2826373548132963 1103 6 0.20061800447430755 1103 7 0.198457811491366 1103 8 0.23393621518579977 1103 9 0.21479435297955973 1103 10-11 0.326928594012422 1103 12-13 0.20457003888226666 1103 14-15 0.20375494249135073 1103 16-17 0.3060410227453545 1103 18-19 0.3218114905440359 1103 20-21 0.3359439895291274 1103 22-23 0.2053859306666439 1103 24-25 0.12865759927905884 1103 26-27 0.28752338792810406 1103 28-29 0.23751790017377772 1103 30-31 0.33435152832706905 1103 32-33 0.4808332538611211 1103 34-35 0.11565448043292292 1103 36-37 0.07149242773379427 1103 38-39 0.10948085974349908 1103 40-41 0.14251726721651892 1103 42-43 -0.0824250340516528 1103 44-45 0.018491040582205187 1103 46-47 0.02494328655760114 1103 48-49 0.018759311603886886 1103 50-51 0.14858015483449094 1103 52-53 0.1921238739063611 1103 54-55 0.36921755315875515 1103 56-57 -0.0018986541288441572 1103 58-59 -0.13822038436490658 1103 60-61 0.022445104214916967 1103 62-63 0.07819993716186247 1103 64-65 0.04193549641481553 1103 66-67 0.03218741257112612 1103 68-69 -0.12068603186153837 1103 70-71 -0.13970469506924843 1103 72-73 -0.06987039102547499 1103 74-75 0.06071765007503416 1103 76-77 0.15736275955306667 1103 78-79 0.17776208457037512 1103 80-81 0.30537294491429634 1103 82-83 0.14676743770702672 1103 84-85 -0.17324054647600207 1103 86-87 -0.09594675626712501 1103 88-89 0.015025014109482981 1103 90-91 0.24192641397379333 1103 92-93 0.2928811819415742 1103 94-95 0.3586790110100395 1103 96-97 0.36031468644676323 1103 98-99 0.28602038074352265 1103 100-101 0.05750425778814261 1104 1 0.2626976426082521 1104 2 0.23423423226191176 1104 3 0.232581818223359 1104 4 0.09591045648537744 1104 5 0.0773723495482912 1104 6 0.08450708836339516 1104 7 0.08413699717055323 1104 8 0.062016243265823334 1104 9 0.2516142897994982 1104 10-11 0.07878889587272653 1104 12-13 0.02022465453688227 1104 14-15 0.1345903233267336 1104 16-17 0.22036183706617152 1104 18-19 0.15870162743416927 1104 20-21 0.017498921084062147 1104 22-23 -0.010058084777377019 1104 24-25 0.13099175161321597 1104 26-27 0.13181963222434945 1104 28-29 0.013544576200459346 1104 30-31 0.016310110285651547 1104 32-33 0.06782869085655818 1104 34-35 -0.034854470076027155 1104 36-37 0.0284597847011554 1104 38-39 -0.10201440175175946 1104 40-41 0.14502691972617043 1104 42-43 0.31238016075354835 1104 44-45 0.10322252531369003 1104 46-47 0.01583482744914022 1104 48-49 0.41475790760248543 1104 50-51 0.06834147459581175 1104 52-53 0.20132008310256566 1104 54-55 0.15917894312014624 1104 56-57 0.22089881866863692 1104 58-59 0.06478066863614629 1104 60-61 0.2295528613226736 1104 62-63 0.024882983844911166 1104 64-65 0.06675122123054678 1104 66-67 0.09064652103023008 1104 68-69 0.06629185511634716 1104 70-71 -0.12257587794042735 1104 72-73 -0.4223975935526809 1104 74-75 -0.23975620039880852 1104 76-77 -0.2770001748098707 1104 78-79 -0.040507483699194324 1104 80-81 0.005478245019595818 1104 82-83 -8.28209888616982E-4 1104 84-85 -0.23657855981401354 1104 86-87 0.12107676075638807 1104 88-89 0.12408082316529345 1104 90-91 -0.005967583920202912 1104 92-93 0.08572077478117279 1104 94-95 0.08488118721221127 1104 96-97 -0.006375380243298423 1104 98-99 0.1373366320597782 1104 100-101 -0.13114088085700004 1105 1 -0.051098238929562 1105 2 0.06243184110468292 1105 3 -0.008306325890593769 1105 4 -0.008204783113733072 1105 5 0.001596512482130663 1105 6 -0.021580372562773675 1105 7 -0.1266779942896008 1105 8 -0.052952658799853225 1105 9 0.015753376519228368 1105 10-11 0.06247617633419367 1105 12-13 -0.002974726727018151 1105 14-15 0.06806652454486795 1105 16-17 0.06988650594568213 1105 18-19 -0.03415390413103836 1105 20-21 -0.17180532305889074 1105 22-23 -0.2878673722640812 1105 24-25 -0.18206938080276558 1105 26-27 -0.17396812840212306 1105 28-29 -0.2148528328421051 1105 30-31 -0.1636164148021635 1105 32-33 0.22173929638006484 1105 34-35 0.060118299090291316 1105 36-37 0.10908883307213557 1105 38-39 -0.05562208439170746 1105 40-41 -0.01647609338974121 1105 42-43 -0.048678135788620125 1105 44-45 -0.16223459820795227 1105 46-47 0.12143245562741356 1105 48-49 0.09124271311954857 1105 50-51 0.16108099314178048 1105 52-53 0.05065784211775082 1105 54-55 0.08707685166321966 1105 56-57 0.052963528152695005 1105 58-59 -0.004865813913561112 1105 60-61 -0.017454969556119693 1105 62-63 0.09666281691506384 1105 64-65 -0.0647173102379881 1105 66-67 0.1071622633524214 1105 68-69 0.194872951439379 1105 70-71 0.17272237219653164 1105 72-73 -0.04949952388042078 1105 74-75 0.12076392463743701 1105 76-77 0.16280840370838945 1105 78-79 0.10189527483260008 1105 80-81 0.24777788086762342 1105 82-83 0.3229125654650602 1105 84-85 0.3742847704041523 1105 86-87 0.16241284402795486 1105 88-89 0.33058399741040034 1105 90-91 0.14573232907003586 1105 92-93 0.22820409790965357 1105 94-95 0.07503941608011644 1105 96-97 0.03255665546293329 1105 98-99 0.07327810993673722 1105 100-101 -0.2536184033345208 1106 1 0.26233637318223657 1106 2 0.2886488823600146 1106 3 0.2594278738463913 1106 4 0.1733591468837048 1106 5 0.22376121248391456 1106 6 0.14897499455791774 1106 7 0.1711780137079728 1106 8 0.1618511805108369 1106 9 0.15120630449942496 1106 10-11 0.21316555039326346 1106 12-13 0.17567639703898408 1106 14-15 0.12661125203830892 1106 16-17 0.0520258564999736 1106 18-19 0.1252530616115024 1106 20-21 0.25580593780834704 1106 22-23 0.2003564407450611 1106 24-25 0.14228453556786747 1106 26-27 -0.028777085206904474 1106 28-29 0.10181889181290416 1106 30-31 0.21037719428078816 1106 32-33 0.6120374796696666 1106 34-35 0.22477367156650274 1106 36-37 0.35164020140675234 1106 38-39 0.3487283806456958 1106 40-41 0.3177230485374025 1106 42-43 0.22069247194355057 1106 44-45 0.4848725299852745 1106 46-47 0.22017628934455757 1106 48-49 0.45778322760622103 1106 50-51 0.17784971216160272 1106 52-53 0.4564572720527096 1106 54-55 0.353046706052659 1106 56-57 0.36189355534682477 1106 58-59 0.3232191126861039 1106 60-61 0.4365322129063429 1106 62-63 0.30661396269819363 1106 64-65 0.4774335844308908 1106 66-67 0.6005035704556221 1106 68-69 0.44987589697377217 1106 70-71 0.47043854169269395 1106 72-73 0.37881390478111143 1106 74-75 0.5627041689680325 1106 76-77 0.3310162691778018 1106 78-79 0.6191549162509773 1106 80-81 0.7620324821493725 1106 82-83 0.5838988136873198 1106 84-85 0.5384938497907754 1106 86-87 0.3501028905019439 1106 88-89 0.4081289093716549 1106 90-91 0.49394209872328787 1106 92-93 0.49518852525611834 1106 94-95 0.5359976966021023 1106 96-97 0.5481233584281 1106 98-99 0.5112909750788646 1106 100-101 0.5024229174549966 1107 1 0.32939721382089004 1107 2 0.2568643337518708 1107 3 0.2274610482569841 1107 4 0.16974208803672042 1107 5 0.07244283725535183 1107 6 0.18781708003442787 1107 7 0.1864721916672707 1107 8 0.21720642125885092 1107 9 0.3653817574862046 1107 10-11 0.23045623091298495 1107 12-13 0.2454058866064699 1107 14-15 0.2554497489367549 1107 16-17 0.254131155633587 1107 18-19 0.2930447190219141 1107 20-21 0.3664793942925684 1107 22-23 0.296558813763518 1107 24-25 0.34042815843678653 1107 26-27 0.2993613541603679 1107 28-29 0.2805557123327347 1107 30-31 0.2597286869583826 1107 32-33 0.5133202539253148 1107 34-35 0.369060142627184 1107 36-37 0.49517477991970793 1107 38-39 0.5663756405100102 1107 40-41 0.5032895596990272 1107 42-43 0.4643683151169995 1107 44-45 0.688831927787696 1107 46-47 0.7393780300897248 1107 48-49 0.6386219295662627 1107 50-51 0.7637050184154077 1107 52-53 0.7881757452551454 1107 54-55 0.8002376468866927 1107 56-57 0.6143528705549883 1107 58-59 0.6749111557262566 1107 60-61 0.6568965390226467 1107 62-63 0.4461526880124822 1107 64-65 0.5680252441197737 1107 66-67 0.7434058756898239 1107 68-69 0.7958319112597252 1107 70-71 0.6886126959559888 1107 72-73 0.8568324400716492 1107 74-75 0.7141136826872199 1107 76-77 0.7859112605481258 1107 78-79 0.7107434031811479 1107 80-81 0.7294533198260282 1107 82-83 0.8386850084844539 1107 84-85 0.7084471982758842 1107 86-87 0.8189790959508798 1107 88-89 0.875723479796072 1107 90-91 0.7963367625646711 1107 92-93 1.0069596309368904 1107 94-95 0.7774406467550392 1107 96-97 0.6714850016550855 1107 98-99 0.8175476324608013 1107 100-101 0.8909092780102661 1108 1 0.13560612947023287 1108 2 0.10146467623654587 1108 3 0.020500875909860383 1108 4 0.10777998230839358 1108 5 -0.12226522218230684 1108 6 -0.16405829973687958 1108 7 0.00996001136566349 1108 8 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-0.02709997354133975 2316 88-89 -0.13510483103815574 2316 90-91 -0.7008166849970578 2316 92-93 -0.2316499328030588 2316 94-95 -0.060138917238504064 2316 96-97 0.06485445753457952 2316 98-99 0.06553370189385532 2316 100-101 0.0921248214122592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 17.0 15 42.0 16 134.0 17 278.0 18 530.0 19 875.0 20 1198.0 21 1827.0 22 2356.0 23 2601.0 24 2354.0 25 2096.0 26 1889.0 27 1963.0 28 2127.0 29 2743.0 30 3291.0 31 4282.0 32 5424.0 33 7281.0 34 10455.0 35 17254.0 36 34432.0 37 87279.0 38 170228.0 39 68559.0 40 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72926189757988 20.419982829431284 12.353528087801935 23.497227185186905 2 14.712839677218314 20.985906682452047 42.84919941708389 21.45205422324575 3 17.338047323706103 27.823123420206986 31.429279718643354 23.409549537443556 4 11.511434747453217 18.496233981970377 40.217504627881276 29.77482664269513 5 12.218301619700526 38.55146736851373 36.2399594090213 12.99027160276445 6 28.340241368045728 39.237482710606244 19.45980635878996 12.962469562558066 7 24.9483924628669 34.00259022341256 24.43799334141137 16.61102397230917 8 23.45750805679957 37.164377245883564 21.563494067739672 17.8146206295772 9 25.3492052091756 15.732479501787438 21.508353354663676 37.40996193437328 10-11 23.007346689124535 26.411127303225268 31.148594954393072 19.432931053257125 12-13 24.320298037871012 24.82884368997945 30.410219103245197 20.440639168904344 14-15 21.58087034286866 26.054797821246783 28.13798152554428 24.226350310340276 16-17 21.003630483083615 28.999728930107988 28.297379889394215 21.69926069741418 18-19 20.673365413798617 28.031754563589057 30.394348771960715 20.90053125065161 20-21 21.47371664623989 27.437601796011794 30.517025274371385 20.571656283376928 22-23 21.123410939639452 27.37840661873904 30.33526943652215 21.162913005099355 24-25 20.99737502403718 27.473396922777514 30.400256705504574 21.128971347680732 26-27 20.904238189345794 27.743308396447823 30.17633444000899 21.17611897419739 28-29 20.909566913718685 27.664535949196406 30.23993160698109 21.185965530103818 30-31 21.08078114465633 27.423700775908603 30.29762084040934 21.197897239025725 32-33 20.74101704496748 27.797290691181885 30.29298716704161 21.16870509680902 34-35 21.078464307972467 27.865174006019146 30.17019482279675 20.886166863211646 36-37 21.18897741779284 27.6353438069797 30.06918074338022 21.106498031847238 38-39 21.063057344024763 27.814782808145072 30.058059927297663 21.064099920532502 40-41 21.08854254754728 27.820806583523122 30.0075528875894 21.0830979813402 42-43 21.05532644455771 27.879384643899723 29.766345396413513 21.29894351512905 44-45 21.11521062965902 27.994022554431247 29.72018372536186 21.170583090547876 46-47 21.175985413128647 27.87584478049029 29.478060613362185 21.470109193018875 48-49 21.15146467168116 28.15086575899151 29.325754056561916 21.371915512765412 50-51 21.271720494879755 28.161808998656223 29.142301098188224 21.424169408275798 52-53 21.341317198789213 28.23383041352299 29.04820322482366 21.376649162864137 54-55 21.224193335387596 28.225673794028584 29.054985485018175 21.495147385565645 56-57 21.23392404945983 28.1496815507978 29.028689388656304 21.587705011086065 58-59 21.20623785108764 28.158485530196494 29.178472880268195 21.45680373844767 60-61 21.436994784800625 28.30873238914516 28.923041635872043 21.331231190182173 62-63 21.1793625455548 28.264712492151716 29.174302574237238 21.381622388056243 64-65 21.1587426990684 28.23783718661888 29.047108240293035 21.556311874019688 66-67 21.296271626247474 28.302973084118644 28.79506976582546 21.605685523808422 68-69 21.180064431601373 28.298238846393996 29.06766476569116 21.454031956313475 70-71 21.171253617161273 28.186287570402875 28.858401892392205 21.78405692004365 72-73 21.34003516958086 28.189762825428673 28.753565032447298 21.716636972543167 74-75 21.192800198321223 28.32124330723803 28.726921410582847 21.759035083857903 76-77 21.228479483252745 28.26888279818267 28.72194021171254 21.780697506852047 78-79 21.179130861886414 28.12025772491271 28.732945185960894 21.967666227239977 80-81 21.179130861886414 28.24177580898145 28.647569754160457 21.931523574971674 82-83 21.188282366787682 28.194859866133175 28.67838368205587 21.938474085023273 84-85 21.0899326495576 28.22382032468149 28.72529962490414 21.960947400856767 86-87 21.091091067899534 28.241659967147253 28.673634166853944 21.993614798099266 88-89 21.207859636766344 28.186866779573844 28.608299372368943 21.99697421129087 90-91 21.302642934508743 28.05391285208718 28.528054028694054 22.11539018471002 92-93 21.21052399895279 27.99885548267817 28.692863911330026 22.097756607039013 94-95 21.04058402819127 27.929929591333174 28.666799498636543 22.36268688183901 96-97 21.092944537246623 28.021560482180053 28.627760800513414 22.257734180059913 98-99 21.130501769486067 27.896136091144463 28.56211156739975 22.411250571969717 100-101 21.051729230983092 27.96019669966232 28.471986515994878 22.516087553359707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 26.0 1 23.5 2 47.0 3 73.5 4 60.0 5 64.5 6 119.5 7 226.0 8 381.0 9 521.5 10 465.0 11 359.5 12 367.0 13 403.0 14 454.0 15 487.0 16 524.0 17 542.0 18 572.0 19 643.5 20 767.5 21 917.5 22 1136.0 23 1476.5 24 1910.5 25 2473.5 26 3239.0 27 4018.0 28 4886.5 29 5853.0 30 6861.5 31 8032.5 32 9336.5 33 10455.0 34 11453.0 35 12244.5 36 13118.5 37 14152.5 38 14718.5 39 15229.0 40 15646.5 41 15962.0 42 15921.0 43 16189.0 44 16580.0 45 16494.5 46 16527.0 47 16412.5 48 16230.0 49 15807.5 50 15010.0 51 13964.5 52 13007.5 53 12275.5 54 11116.0 55 9757.0 56 8528.0 57 7145.0 58 5913.5 59 5041.5 60 4165.5 61 3329.0 62 2630.5 63 1946.5 64 1486.0 65 1199.0 66 957.0 67 782.0 68 668.0 69 571.5 70 450.0 71 344.0 72 262.5 73 195.0 74 154.5 75 116.0 76 72.5 77 48.0 78 30.0 79 20.0 80 16.0 81 9.0 82 4.5 83 3.5 84 2.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15128943545640525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 6.950510051595953E-4 44-45 1.1584183419326589E-4 46-47 4.6336733677306355E-4 48-49 0.0017376275128989883 50-51 6.950510051595953E-4 52-53 5.792091709663294E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1584183419326589E-4 68-69 5.792091709663294E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.1584183419326589E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.1584183419326589E-4 100-101 1.1584183419326589E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 431623.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.6348861302611 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12997133595334 55.187016286756275 2 14.096480254162355 19.91406555180464 3 4.178573423214178 8.854591738070063 4 1.662770968062156 4.697985519590976 5 0.7736383200161767 2.7322927320174566 6 0.39195120674920736 1.6611257314409211 7 0.23760439186761997 1.1748211414533491 8 0.13395858755523377 0.7569719662507638 9 0.09330476709360488 0.5931514439160581 >10 0.29152006436577815 3.0291618602161696 >50 0.007587537539754359 0.3688698661476224 >100 0.0016494646378518551 0.1752306592933194 >500 3.2989292757037064E-4 0.17290046435590808 >1k 6.597858551407413E-4 0.6818150386865055 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1478 0.34242846187529397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1448 0.335477951823698 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 742 0.17190928194280658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.6336733677306355E-4 0.0 0.0 0.0 0.0 6 4.6336733677306355E-4 0.0 0.0 0.0 0.0 7 4.6336733677306355E-4 0.0 0.0 0.0 0.0 8 4.6336733677306355E-4 0.0 0.0 0.0 0.0 9 4.6336733677306355E-4 0.0 0.0 0.0 0.0 10-11 9.267346735461271E-4 0.0 0.0 0.0 0.0 12-13 0.0011584183419326589 0.0 0.0 0.0 0.0 14-15 0.0013901020103191906 0.0 0.0 1.1584183419326589E-4 0.0 16-17 0.00196931118128552 0.0 0.0 2.3168366838653177E-4 0.0 18-19 0.0023168366838653177 0.0 0.0 2.3168366838653177E-4 0.0 20-21 0.0023168366838653177 0.0 0.0 2.3168366838653177E-4 0.0 22-23 0.0023168366838653177 0.0 0.0 2.3168366838653177E-4 0.0 24-25 0.002896045854831647 0.0 0.0 8.108928393528612E-4 0.0 26-27 0.0035910968599912425 0.0 0.0 0.001042576507739393 0.0 28-29 0.0037069386941845084 0.0 0.0 0.0013901020103191906 0.0 30-31 0.0037069386941845084 0.0 0.0 0.0035910968599912425 0.0 32-33 0.004401989699344104 0.0 0.0 0.009383188569654536 1.1584183419326589E-4 34-35 0.004865357036117167 0.0 0.0 0.01598617311867069 2.3168366838653177E-4 36-37 0.005328724372890231 0.0 0.0 0.02560104535671176 2.3168366838653177E-4 38-39 0.005328724372890231 0.0 0.0 0.0448307898327939 2.3168366838653177E-4 40-41 0.005328724372890231 0.0 0.0 0.0722853045365979 2.3168366838653177E-4 42-43 0.005328724372890231 0.0 0.0 0.09950813557201539 2.3168366838653177E-4 44-45 0.0056762498754700285 0.0 0.0 0.12881611962291167 2.3168366838653177E-4 46-47 0.006023775378049826 0.0 0.0 0.17272017478215942 3.4752550257979766E-4 48-49 0.006023775378049826 0.0 0.0 0.21616086260463413 4.6336733677306355E-4 50-51 0.006023775378049826 0.0 0.0 0.26666790231289805 4.6336733677306355E-4 52-53 0.006255459046436358 0.0 0.0 0.32018682971018686 9.267346735461271E-4 54-55 0.006255459046436358 0.0 0.0 0.36837703273458555 9.267346735461271E-4 56-57 0.006255459046436358 0.0 0.0 0.4352177710640999 0.001042576507739393 58-59 0.00648714271482289 0.0 0.0 0.5115575397974621 0.0011584183419326589 60-61 0.00648714271482289 0.0 0.0 0.6019141704682095 0.0011584183419326589 62-63 0.00648714271482289 0.0 0.0 0.6891430716157387 0.0011584183419326589 64-65 0.00648714271482289 0.0 0.0 0.7834383246490572 0.0011584183419326589 66-67 0.00648714271482289 0.0 0.0 0.8945306436403992 0.0011584183419326589 68-69 0.0067188263832094215 0.0 0.0 1.0305289569832934 0.0011584183419326589 70-71 0.0067188263832094215 0.0 0.0 1.1796173975900266 0.0011584183419326589 72-73 0.0067188263832094215 0.0 0.0 1.3355405064141623 0.0011584183419326589 74-75 0.0067188263832094215 0.0 0.0 1.492737875414424 0.0011584183419326589 76-77 0.0067188263832094215 0.0 0.0 1.6601293258236933 0.0011584183419326589 78-79 0.006950510051595953 0.0 0.0 1.8654010560141605 0.0011584183419326589 80-81 0.006950510051595953 0.0 0.0 2.0841104389710465 0.0011584183419326589 82-83 0.007182193719982485 0.0 0.0 2.325756505098199 0.0013901020103191906 84-85 0.007413877388369017 0.0 0.0 2.5742372394427546 0.0013901020103191906 86-87 0.007413877388369017 0.0 0.0 2.83337542253309 0.0013901020103191906 88-89 0.007413877388369017 0.0 0.0 3.1469592676942613 0.0013901020103191906 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 785 0.0 58.19541 1 GTATCAA 1460 0.0 34.549377 1 TCAACGC 1520 0.0 32.49909 4 ATCAACG 1525 0.0 32.392532 3 CAACGCA 1580 0.0 31.264946 5 TATCAAC 1605 0.0 31.073895 2 AACGCAG 1655 0.0 30.709108 6 CGCAGAG 1780 0.0 28.285725 8 ACGCAGA 1785 0.0 28.206491 7 GTACTAT 125 7.2729217E-6 26.648647 1 GTATAGG 185 8.762072E-9 25.722635 1 TATAGGG 115 1.280185E-4 24.781916 2 GTACATG 1125 0.0 24.110682 1 GCAGAGT 2035 0.0 24.041094 9 GTGGTAT 280 1.8189894E-12 23.793436 1 GAGTACT 1380 0.0 23.749334 12-13 CAGAGTA 2020 0.0 22.926336 10-11 TTATATC 235 4.629328E-9 22.23342 3 GCGAGAG 65 2.9412445E-4 21.922462 36-37 CTAGACA 175 4.040541E-6 21.713676 4 >>END_MODULE