FastQCFastQC Report
Fri 10 Feb 2017
SRR2031809_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031809_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419520
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.42667810831426395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.4002192982456141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.23908276125095348No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA250.0050190657.07
GGTATCA13050.040.40231
GTATCAA19400.032.0747451
GCGTACA600.00394811731.6666689
ATTAACG650.00583989729.230773
TCAACGC22750.026.9340654
CAACGCA23200.026.4116385
ATCAACG23350.026.241973
AACGCAG23950.026.179546
TATCAAC24000.025.5312482
TACATGG14000.024.7678572
GTACATG13950.024.516131
CGCACGG500.001653275123.7570-71
ACGCAGA26350.023.2542697
CGCAGAG26150.022.887198
ACATGGG14500.022.6034493
GAGTACT15300.022.5081712-13
CATGGGG7600.021.8750024
AGAGTAC22300.021.72645810-11
GTATTAG1553.8988182E-521.4516121
Produced by FastQC (version 0.11.5)