##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031809_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419520 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54855072463768 34.0 31.0 34.0 31.0 34.0 2 32.67171290999237 34.0 31.0 34.0 31.0 34.0 3 32.73695652173913 34.0 33.0 34.0 31.0 34.0 4 35.91927679252479 37.0 37.0 37.0 35.0 37.0 5 35.950424294431734 37.0 37.0 37.0 35.0 37.0 6 36.02928823417239 37.0 37.0 37.0 35.0 37.0 7 36.04234839816934 37.0 37.0 37.0 35.0 37.0 8 36.04170242181541 37.0 37.0 37.0 35.0 37.0 9 37.87970537757437 39.0 39.0 39.0 37.0 39.0 10-11 37.9133843440122 39.0 39.0 39.0 37.0 39.0 12-13 37.88284229595729 39.0 39.0 39.0 37.0 39.0 14-15 39.4494469870328 41.0 40.0 41.0 37.0 41.0 16-17 39.3803513539283 41.0 40.0 41.0 37.0 41.0 18-19 39.38794336384439 41.0 40.0 41.0 37.0 41.0 20-21 39.329935402364605 41.0 40.0 41.0 37.0 41.0 22-23 39.23766924103738 41.0 40.0 41.0 36.0 41.0 24-25 39.19374165713196 41.0 40.0 41.0 36.0 41.0 26-27 39.06540093440122 41.0 39.0 41.0 35.5 41.0 28-29 38.929639826468346 41.0 39.0 41.0 35.0 41.0 30-31 38.764560688405794 41.0 39.0 41.0 35.0 41.0 32-33 38.61630792334096 41.0 38.5 41.0 35.0 41.0 34-35 38.40898884439359 40.0 38.0 41.0 35.0 41.0 36-37 38.28616156559878 40.0 38.0 41.0 34.0 41.0 38-39 38.09057732646835 40.0 38.0 41.0 34.0 41.0 40-41 37.96664402173913 40.0 38.0 41.0 33.0 41.0 42-43 37.77853737604882 40.0 38.0 41.0 33.0 41.0 44-45 37.89609792143402 40.0 38.0 41.0 33.0 41.0 46-47 37.734445318459194 40.0 38.0 41.0 33.0 41.0 48-49 37.531470490083905 40.0 38.0 41.0 32.5 41.0 50-51 36.455052202517166 39.0 36.5 40.0 30.5 40.5 52-53 36.457627765064835 39.5 36.5 40.0 30.5 40.5 54-55 37.192671386346305 40.0 37.0 41.0 31.5 41.0 56-57 37.15636203279939 40.0 37.0 41.0 31.0 41.0 58-59 37.01817195842868 40.0 36.0 41.0 31.0 41.0 60-61 36.79805134439359 40.0 35.5 41.0 31.0 41.0 62-63 36.51411136536995 39.0 35.0 41.0 30.5 41.0 64-65 36.18841175629291 39.0 35.0 41.0 30.0 41.0 66-67 35.894440074370706 38.5 35.0 41.0 29.5 41.0 68-69 35.556892400839054 37.0 35.0 40.5 29.0 41.0 70-71 35.19915021929825 37.0 35.0 39.5 29.0 41.0 72-73 34.80345513920672 36.0 35.0 39.0 29.0 41.0 74-75 34.45902459954233 36.0 35.0 39.0 28.0 41.0 76-77 34.10174008390542 35.0 35.0 37.5 28.0 40.0 78-79 33.744602164378335 35.0 34.5 37.0 27.0 39.0 80-81 33.40911398741419 35.0 34.0 37.0 26.0 39.0 82-83 33.139551153699465 35.0 34.0 36.0 26.0 38.0 84-85 32.85315241228071 35.0 34.0 36.0 26.0 37.0 86-87 32.6137037566743 35.0 34.0 35.5 25.0 37.0 88-89 32.420140875286044 35.0 34.0 35.0 25.0 36.5 90-91 32.254182160564454 35.0 34.0 35.0 24.5 36.0 92-93 32.12370327993898 35.0 34.0 35.0 24.0 36.0 94-95 31.969826229977116 35.0 34.0 35.0 23.5 36.0 96-97 31.862200848588863 35.0 33.0 35.0 23.0 35.5 98-99 31.74474160945843 35.0 33.0 35.0 22.5 35.0 100-101 30.24426844965675 34.0 30.0 35.0 15.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07950040854989737 1101 2 0.037209539751273724 1101 3 0.10575894047693879 1101 4 0.1174852840883105 1101 5 -0.004862397785295514 1101 6 -0.009227299159825009 1101 7 0.024679987904690392 1101 8 0.25971275642071845 1101 9 0.20873290249497956 1101 10-11 0.3411587684160011 1101 12-13 0.30830858859687993 1101 14-15 0.3086612843131391 1101 16-17 0.35926996757711294 1101 18-19 0.3488954004877698 1101 20-21 0.41796978067524293 1101 22-23 0.4248875268590311 1101 24-25 0.3739923599883923 1101 26-27 0.2249300143252384 1101 28-29 0.37901934023759765 1101 30-31 0.3263262025362792 1101 32-33 0.017752415233864838 1101 34-35 0.29120668617415646 1101 36-37 0.3355415083621338 1101 38-39 0.3757900625565114 1101 40-41 0.46249977545353005 1101 42-43 0.44263980197583663 1101 44-45 0.6590527561646695 1101 46-47 0.7442091610265322 1101 48-49 0.29731814396760825 1101 50-51 0.7688792981167936 1101 52-53 0.8906964048161399 1101 54-55 0.6396657132613655 1101 56-57 0.739167238876334 1101 58-59 0.7612516215912422 1101 60-61 0.7099862323350195 1101 62-63 0.57111815485667 1101 64-65 0.4400193626933202 1101 66-67 0.4280227144288915 1101 68-69 0.37099631885237017 1101 70-71 0.651702445314065 1101 72-73 0.593853326825986 1101 74-75 0.46806688751971137 1101 76-77 0.22447412448617854 1101 78-79 0.5041949341161072 1101 80-81 0.6797793474378793 1101 82-83 0.6374728809694759 1101 84-85 0.385365379478138 1101 86-87 0.38866148070894013 1101 88-89 0.36834697605189604 1101 90-91 0.4320360717523286 1101 92-93 0.13758756260816796 1101 94-95 0.16914706551514058 1101 96-97 0.11040718695749874 1101 98-99 0.042701495205481166 1101 100-101 -9.247214997998299E-4 1102 1 0.19557735805178567 1102 2 0.18646237382890973 1102 3 0.21123815724093475 1102 4 0.16633447680558788 1102 5 0.15470476126657928 1102 6 0.2137716702664889 1102 7 0.04960269487824576 1102 8 0.2881565901548342 1102 9 0.073590339801747 1102 10-11 0.23001569043591985 1102 12-13 0.16869591254054228 1102 14-15 0.1723979727363627 1102 16-17 0.18648570100204864 1102 18-19 0.29702319162483093 1102 20-21 0.483479914649827 1102 22-23 0.41249917234168265 1102 24-25 0.322661202310627 1102 26-27 0.28918198959146935 1102 28-29 0.4500988661736969 1102 30-31 0.1778462987231606 1102 32-33 0.3905495639799952 1102 34-35 0.32756876106250843 1102 36-37 0.22828884604677313 1102 38-39 0.5493944940233604 1102 40-41 0.3324242000498927 1102 42-43 0.4529541269500683 1102 44-45 0.23436622232750892 1102 46-47 0.3159413836304523 1102 48-49 0.29075253077626684 1102 50-51 0.24131574607282857 1102 52-53 0.34882076070758217 1102 54-55 0.2922378534193868 1102 56-57 0.5446163972411568 1102 58-59 0.3377158950367942 1102 60-61 0.5234094545954164 1102 62-63 0.35291135307034693 1102 64-65 0.7372411077980843 1102 66-67 0.5829308216085494 1102 68-69 0.6343422480863055 1102 70-71 0.3909028918959976 1102 72-73 0.5574955459946551 1102 74-75 0.41767612833043444 1102 76-77 0.32655674214333885 1102 78-79 0.18095549749639162 1102 80-81 0.324720948262339 1102 82-83 0.45735007093855273 1102 84-85 0.5178842733290452 1102 86-87 0.6727391172599511 1102 88-89 0.5678445713799576 1102 90-91 0.5917792871422307 1102 92-93 0.4730908260915072 1102 94-95 0.5584162170094729 1102 96-97 0.5705411615366778 1102 98-99 0.543126606850965 1102 100-101 0.4196309294792471 1103 1 0.2836361455594414 1103 2 0.24326733402487122 1103 3 0.28126265203579237 1103 4 0.3267906923597863 1103 5 0.18654799457949878 1103 6 0.2985006965983956 1103 7 0.17764923498847196 1103 8 0.3012689600262348 1103 9 0.2986056490485325 1103 10-11 0.26696073023996547 1103 12-13 0.2118411972925358 1103 14-15 0.2633272440162173 1103 16-17 0.2766877830596215 1103 18-19 0.19877610038172122 1103 20-21 0.3792966629658068 1103 22-23 0.5383220443563772 1103 24-25 0.5022731129046178 1103 26-27 0.38896766013647976 1103 28-29 0.3994329139385471 1103 30-31 0.3636737635118905 1103 32-33 0.15726726146927916 1103 34-35 0.31831432138093874 1103 36-37 0.12756165682448284 1103 38-39 0.34304881123095043 1103 40-41 0.2402701890272354 1103 42-43 0.34239457398008 1103 44-45 0.3904286840543847 1103 46-47 0.48118556611667884 1103 48-49 0.30056575796548657 1103 50-51 0.21101874668519116 1103 52-53 0.49776824999111113 1103 54-55 0.6381081840991172 1103 56-57 0.4561940151223567 1103 58-59 0.6243547499051303 1103 60-61 0.6085480032788197 1103 62-63 0.5496772746869993 1103 64-65 0.37032821741229327 1103 66-67 0.4468124811308982 1103 68-69 0.37904907601036797 1103 70-71 0.34616161816666846 1103 72-73 0.5377159991483538 1103 74-75 0.4956384675833476 1103 76-77 0.4222471891733477 1103 78-79 0.28952102107263045 1103 80-81 0.2055614259108438 1103 82-83 0.316091650852826 1103 84-85 0.3016564717368908 1103 86-87 0.43565246692315185 1103 88-89 0.43873403861816485 1103 90-91 0.3951855945519043 1103 92-93 0.2844923876346783 1103 94-95 0.2007615936169458 1103 96-97 0.3503329557803987 1103 98-99 0.7285445492881983 1103 100-101 0.3776874205998837 1104 1 0.06957104696119387 1104 2 0.09796856947271948 1104 3 0.07640401578014888 1104 4 0.28161963034980886 1104 5 0.15447386770851068 1104 6 0.14167900498518549 1104 7 0.12407356380766288 1104 8 0.21293146893571446 1104 9 0.3186222800772853 1104 10-11 0.19146031446425127 1104 12-13 0.23009820608321974 1104 14-15 0.4611919387180521 1104 16-17 0.46741004487952154 1104 18-19 0.26766122749030075 1104 20-21 0.3409339283495072 1104 22-23 0.26261077089475293 1104 24-25 0.2903091665990871 1104 26-27 0.27813373283783704 1104 28-29 0.2879307756634262 1104 30-31 0.24840737006926616 1104 32-33 0.251767142676222 1104 34-35 0.19127999018589037 1104 36-37 0.11632086328682334 1104 38-39 -0.004049668217845692 1104 40-41 0.018943864484590733 1104 42-43 0.15323889567169147 1104 44-45 0.19133151132928106 1104 46-47 0.21073088567001008 1104 48-49 0.14147214903224636 1104 50-51 0.007258946257543641 1104 52-53 0.3297874350706991 1104 54-55 0.35047305699053766 1104 56-57 0.4132725967331865 1104 58-59 0.31109001956538407 1104 60-61 0.27900862337860133 1104 62-63 0.3698320026508881 1104 64-65 0.49790454195494505 1104 66-67 0.34364813092895474 1104 68-69 0.18900244973334424 1104 70-71 0.45582246397327 1104 72-73 0.45355111437762474 1104 74-75 0.22780465907776204 1104 76-77 0.3787540791709745 1104 78-79 0.22277416908878678 1104 80-81 0.46997993386997194 1104 82-83 0.35692676608208984 1104 84-85 0.14823523153731344 1104 86-87 0.25854685989059334 1104 88-89 0.3808669680550878 1104 90-91 0.3949925558253682 1104 92-93 0.3593988380979667 1104 94-95 0.4000963524670311 1104 96-97 0.4365767191446217 1104 98-99 0.21632371298503372 1104 100-101 0.39077693117246426 1105 1 0.041318822545555633 1105 2 -0.00999069010442355 1105 3 0.24683287739431847 1105 4 0.37319975222775525 1105 5 0.23412368317499244 1105 6 0.21152994767965794 1105 7 0.2594839720701927 1105 8 0.2942549604815028 1105 9 0.3790176681698654 1105 10-11 0.2694006915420317 1105 12-13 0.32237926922515214 1105 14-15 0.36298863714164753 1105 16-17 0.5381264354611659 1105 18-19 0.5482952217111148 1105 20-21 0.4166928045546996 1105 22-23 0.522280899352964 1105 24-25 0.6503140302731651 1105 26-27 0.6663407536944135 1105 28-29 0.6315493491764315 1105 30-31 0.7231305697946127 1105 32-33 0.6739984246834823 1105 34-35 0.6336597573017286 1105 36-37 0.6767734710744264 1105 38-39 0.681442278110076 1105 40-41 0.604244124834203 1105 42-43 0.7419151180274284 1105 44-45 0.728352654006585 1105 46-47 0.9861721925678495 1105 48-49 0.7838061403920733 1105 50-51 0.7124130102863759 1105 52-53 0.6806657573892423 1105 54-55 0.8381636700995685 1105 56-57 0.8775675070413129 1105 58-59 0.6939469989376832 1105 60-61 0.5242021689970002 1105 62-63 0.7179065599134944 1105 64-65 0.8353791507152835 1105 66-67 0.8298152696580985 1105 68-69 0.7806183339206783 1105 70-71 0.5923629612124159 1105 72-73 0.7304658284222043 1105 74-75 0.8364505549951389 1105 76-77 0.6772978379327412 1105 78-79 0.5918912691946403 1105 80-81 0.6023147835252232 1105 82-83 0.5788676581371419 1105 84-85 0.3476387162167427 1105 86-87 0.34859635492679075 1105 88-89 0.41976920820614794 1105 90-91 0.5784174406707265 1105 92-93 0.3711145706531127 1105 94-95 0.3141237607009444 1105 96-97 0.6541116946832055 1105 98-99 0.43616178124813487 1105 100-101 0.44173106958690767 1106 1 0.06384840163612182 1106 2 0.067849383551291 1106 3 0.19952694508612012 1106 4 0.07997168060582283 1106 5 0.1296040256190949 1106 6 0.10710648228823061 1106 7 0.08435926579983288 1106 8 0.12188547263607319 1106 9 0.18280626527585753 1106 10-11 0.15571529488677527 1106 12-13 0.12605793242889973 1106 14-15 0.025216722505319922 1106 16-17 0.24918578602663644 1106 18-19 0.2319054510607117 1106 20-21 0.10505767517623354 1106 22-23 0.1844299697244054 1106 24-25 0.30086163852369907 1106 26-27 0.24966034528996062 1106 28-29 0.2747966592312636 1106 30-31 0.06175447217330543 1106 32-33 0.170875614772946 1106 34-35 0.05247357049654511 1106 36-37 0.15838015505568137 1106 38-39 0.24133564222490378 1106 40-41 0.29088784279877444 1106 42-43 0.29712254955921935 1106 44-45 0.3376784843510876 1106 46-47 0.1312162348374315 1106 48-49 -0.21653740305242763 1106 50-51 -0.16497976980459583 1106 52-53 -0.024865979154157003 1106 54-55 0.08636006701592436 1106 56-57 0.020787333620589266 1106 58-59 0.09484502150161234 1106 60-61 0.24168234654025156 1106 62-63 0.18052216228257123 1106 64-65 0.17149862138353456 1106 66-67 -0.021224292804966183 1106 68-69 -0.14622408957219335 1106 70-71 0.06825308227713123 1106 72-73 0.31718650011834626 1106 74-75 0.3373094136063912 1106 76-77 0.2791241148837216 1106 78-79 0.17151776876178104 1106 80-81 0.18377236965157095 1106 82-83 0.10310808358133983 1106 84-85 0.21566993744497864 1106 86-87 0.18587356700302848 1106 88-89 0.21082264982437948 1106 90-91 -5.906806962983069E-4 1106 92-93 0.1599452861853976 1106 94-95 0.1942855009546136 1106 96-97 0.3315037876872786 1106 98-99 0.285823734498738 1106 100-101 0.5512046236125485 1107 1 0.08717881866732569 1107 2 0.19713877793254397 1107 3 0.1963190127443255 1107 4 0.023959237716738357 1107 5 0.11537247123006011 1107 6 0.16230236058121505 1107 7 0.15730570305073144 1107 8 0.1102397061346636 1107 9 0.0886485905407497 1107 10-11 0.2687220023816792 1107 12-13 0.38006784785613235 1107 14-15 0.41952021234687464 1107 16-17 0.410933623102558 1107 18-19 0.3248966049442572 1107 20-21 0.3632573446619958 1107 22-23 0.39904064339019385 1107 24-25 0.23488516336087173 1107 26-27 0.24009487430716803 1107 28-29 0.26913903310119025 1107 30-31 0.2913743807958369 1107 32-33 0.4378630746737926 1107 34-35 0.2890537202000516 1107 36-37 0.11178736804294687 1107 38-39 0.33120635283052735 1107 40-41 0.2238517778624427 1107 42-43 0.14862444515559758 1107 44-45 0.38261870015745103 1107 46-47 0.4700711002014799 1107 48-49 0.6356838470690818 1107 50-51 0.5496795088966806 1107 52-53 0.40924503474688123 1107 54-55 0.23089200052422854 1107 56-57 0.31822098774715357 1107 58-59 0.37155348849879033 1107 60-61 0.42351378427720476 1107 62-63 0.17123860050556772 1107 64-65 0.19484848042893788 1107 66-67 0.2178638767293961 1107 68-69 0.3038779810023229 1107 70-71 0.2584737491382114 1107 72-73 0.1750750973770252 1107 74-75 0.3413241895371826 1107 76-77 0.08519002331183145 1107 78-79 0.09493567485684906 1107 80-81 0.18178928420392282 1107 82-83 -0.07154925091851538 1107 84-85 0.02650886035738864 1107 86-87 0.053385961287077066 1107 88-89 0.27408696400732424 1107 90-91 0.280511681508429 1107 92-93 0.2796983726051607 1107 94-95 0.33052541540975255 1107 96-97 0.31216870457266666 1107 98-99 0.41742672856893037 1107 100-101 0.2545292080395001 1108 1 -0.0956423003240019 1108 2 0.06343770096905388 1108 3 -0.12204190136126414 1108 4 -0.14803971158717388 1108 5 -0.18029340741453836 1108 6 -0.036226664898158845 1108 7 0.0032736568201059413 1108 8 -0.02824203802084213 1108 9 -0.0535631247206112 1108 10-11 -0.0464868234654503 1108 12-13 -0.15696918918089864 1108 14-15 -0.04874876585119381 1108 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 192.0 8 466.0 9 385.0 10 424.0 11 498.0 12 540.0 13 619.0 14 611.0 15 695.0 16 730.0 17 1005.0 18 1304.0 19 1714.0 20 2374.0 21 2965.0 22 3424.0 23 3413.0 24 3234.0 25 2870.0 26 2725.0 27 2738.0 28 2961.0 29 3558.0 30 4174.0 31 5120.0 32 6558.0 33 8799.0 34 12979.0 35 22325.0 36 42434.0 37 93857.0 38 136927.0 39 46829.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69636727688787 19.082522883295194 13.791475972540045 23.429633867276888 2 15.905797101449275 21.118659420289855 39.05034324942792 23.925200228832953 3 17.71453089244851 25.899599542334094 31.44927536231884 24.93659420289855 4 12.594631960335622 18.08543096872616 38.13668001525553 31.183257055682684 5 13.324513729977117 37.6756769641495 35.07746948893974 13.922339816933638 6 30.14897978642258 37.63706140350877 17.8654176201373 14.34854118993135 7 25.737509534706334 33.58242753623188 23.00557780320366 17.674485125858126 8 25.575657894736842 34.01029748283753 21.198274218154083 19.21577040427155 9 26.105787566742944 15.058876811594201 20.835240274599542 38.000095347063315 10-11 23.86942219679634 25.72642543859649 29.871162280701753 20.532990083905418 12-13 25.16304347826087 24.60323703279939 28.727355072463766 21.50636441647597 14-15 22.658991228070175 26.02700705568268 26.725901029748282 24.588100686498855 16-17 21.621019260106788 28.60054347826087 27.466390160183067 22.312047101449277 18-19 21.60314168573608 27.49880816170862 29.674747330282226 21.223302822273073 20-21 22.559711098398168 26.73913043478261 29.608481121281464 21.09267734553776 22-23 22.336744775868585 26.753941115967194 29.22212197525979 21.68719213290443 24-25 22.08097597370325 27.08920562691365 29.301736868012096 21.52808153137101 26-27 21.88989797864226 27.123975019069412 29.40157799389779 21.584549008390542 28-29 21.90455758962624 27.008962623951184 29.442458047292142 21.644021739130434 30-31 22.014683447749807 27.035302250190696 29.359506102212052 21.590508199847445 32-33 21.843773836765827 27.090484363081618 29.364035087719298 21.701706712433257 34-35 22.064025553012968 27.207999618611744 29.16392543859649 21.564049389778795 36-37 22.08786231884058 27.148765255530126 29.056540808543097 21.706831617086195 38-39 22.021715293668954 27.302393211289093 29.080377574370708 21.595513920671245 40-41 22.086789664378337 27.217772692601066 29.122449466056445 21.57298817696415 42-43 22.052345537757436 27.2279033180778 29.02245423340961 21.69729691075515 44-45 22.034706331045005 27.460907704042715 28.69827421815408 21.8061117467582 46-47 22.071414950419527 27.38176964149504 28.526172768878716 22.020642639206713 48-49 22.05711289092296 27.49070366132723 28.45907227307399 21.99311117467582 50-51 22.05997330282227 27.762204424103736 28.14406941266209 22.033752860411898 52-53 22.1900028604119 27.663281845919148 28.08114035087719 22.065574942791763 54-55 22.12540522501907 27.614535659801675 28.17946700991609 22.080592105263158 56-57 21.985006674294432 27.706903127383676 28.031083142639208 22.277007055682684 58-59 22.04555205949657 27.647668764302058 28.245971586575138 22.06080758962624 60-61 22.16092200610221 27.641113653699467 28.214387871853546 21.983576468344776 62-63 21.870947749809304 27.604285850495803 28.367658276125095 22.157108123569795 64-65 21.97034706331045 27.55804252479024 28.298650839054158 22.172959572845155 66-67 21.858075896262395 27.76387299771167 28.103785278413422 22.27426582761251 68-69 21.94150457665904 27.74003623188406 28.026434973302823 22.29202421815408 70-71 22.02505244088482 27.44338768115942 28.146095537757436 22.38546434019832 72-73 21.87940980167811 27.712743135011443 27.911303394355453 22.496543668954995 74-75 21.867372234935164 27.852664950419527 27.830615942028984 22.449346872616324 76-77 21.93399599542334 27.883652745995423 27.761608504958048 22.420742753623188 78-79 21.955091533180777 27.77483790999237 27.639683447749807 22.63038710907704 80-81 21.87118611746758 27.907608695652176 27.617038520213576 22.604166666666668 82-83 21.878456331045005 27.785326086956523 27.852903318077804 22.48331426392067 84-85 21.829233409610985 27.82656369183829 27.643020594965673 22.70118230358505 86-87 21.925176392067126 27.866251906941265 27.599160945842865 22.609410755148744 88-89 21.918502097635393 27.760774218154083 27.674365942028984 22.646357742181543 90-91 22.022192028985508 27.554467009916095 27.715007627765065 22.708333333333332 92-93 22.19477021357742 27.544693935926773 27.647907131960338 22.61262871853547 94-95 21.978451563691838 27.515017162471395 27.79331140350877 22.713219870327993 96-97 21.979285850495806 27.485459572845155 27.786279557589626 22.748975019069412 98-99 22.092510488176963 27.491537948131196 27.710121090770407 22.705830472921434 100-101 22.345418573607933 27.305134439359268 27.679848398169337 22.66959858886346 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 420.0 1 264.0 2 141.0 3 174.0 4 159.5 5 141.0 6 194.5 7 403.0 8 683.5 9 823.5 10 700.5 11 556.5 12 545.0 13 587.5 14 621.0 15 631.0 16 678.0 17 726.5 18 762.5 19 827.0 20 966.0 21 1129.5 22 1329.0 23 1578.0 24 1955.0 25 2428.5 26 2879.0 27 3376.0 28 4043.0 29 4880.0 30 5711.0 31 6492.0 32 7278.0 33 8053.0 34 8644.5 35 9192.0 36 10193.5 37 10996.5 38 11474.5 39 11903.0 40 12048.5 41 12429.5 42 12884.5 43 13301.0 44 14006.0 45 14536.5 46 14741.5 47 14974.0 48 15318.0 49 15637.5 50 15564.5 51 15029.5 52 14334.5 53 13756.5 54 13146.0 55 12262.5 56 11386.5 57 10523.5 58 9697.5 59 8505.0 60 6803.5 61 5476.0 62 4378.0 63 3291.5 64 2473.0 65 1865.0 66 1388.5 67 1135.5 68 983.0 69 771.5 70 598.5 71 470.5 72 348.0 73 262.5 74 227.0 75 176.5 76 122.0 77 84.0 78 57.0 79 42.0 80 28.0 81 16.0 82 17.0 83 19.0 84 14.0 85 10.0 86 7.0 87 7.0 88 7.0 89 4.0 90 4.0 91 4.5 92 2.5 93 2.0 94 3.5 95 3.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1918382913806256E-4 24-25 1.1918382913806256E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 419520.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.37384774144674 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76731023050661 54.72784849423525 2 14.64212002464097 20.608446504521442 3 4.240688670420486 8.953007364331485 4 1.5989801789910212 4.501055506316215 5 0.7043259540224696 2.4783063724363243 6 0.34070982722701043 1.4386236903172955 7 0.20703129874199322 1.0198712366768103 8 0.12892913743177675 0.7258591589647935 9 0.07786950898101827 0.493197927185826 >10 0.27870708521480925 3.088180308077682 >50 0.009227134972283911 0.4461268065385623 >100 0.003075711637157193 0.4439623076472315 >500 0.0 0.0 >1k 0.0010252372123857306 1.0755143227510393 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1790 0.42667810831426395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1679 0.4002192982456141 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1003 0.23908276125095348 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.5755148741418763E-4 0.0 22-23 0.0 0.0 0.0 0.0017877574370709383 0.0 24-25 0.0 0.0 0.0 0.0036946987032799392 0.0 26-27 0.0 0.0 0.0 0.008104500381388253 0.0 28-29 0.0 0.0 0.0 0.024551868802440886 0.0 30-31 0.0 0.0 0.0 0.04803108314263921 0.0 32-33 0.0 0.0 0.0 0.07663520213577421 0.0 34-35 0.0 0.0 0.0 0.1215675057208238 0.0 36-37 0.0 0.0 0.0 0.18401983218916856 0.0 38-39 0.0 0.0 0.0 0.2682827993897788 0.0 40-41 0.0 0.0 0.0 0.3687547673531655 0.0 42-43 0.0 0.0 0.0 0.44562833714721584 0.0 44-45 0.0 0.0 0.0 0.5304872234935164 0.0 46-47 0.0 0.0 0.0 0.6387061403508771 0.0 48-49 0.0 0.0 0.0 0.7503813882532417 0.0 50-51 0.0 0.0 0.0 0.8637252097635393 0.0 52-53 0.0 0.0 0.0 0.960502479023646 0.0 54-55 0.0 0.0 0.0 1.0875524408848207 0.0 56-57 0.0 0.0 0.0 1.2548865369946605 0.0 58-59 0.0 0.0 0.0 1.4132818459191459 0.0 60-61 0.0 0.0 0.0 1.5804967581998475 0.0 62-63 0.0 0.0 0.0 1.7362700228832952 0.0 64-65 0.0 0.0 0.0 1.8900171624713957 0.0 66-67 0.0 0.0 0.0 2.0956092677345537 0.0 68-69 0.0 0.0 0.0 2.3412471395881003 0.0 70-71 0.0 0.0 0.0 2.587719298245614 0.0 72-73 0.0 0.0 0.0 2.8364559496567505 0.0 74-75 0.0 0.0 0.0 3.108314263920671 0.0 76-77 0.0 0.0 0.0 3.387323607932876 0.0 78-79 0.0 0.0 0.0 3.721038329519451 0.0 80-81 0.0 0.0 0.0 4.067267353165523 0.0 82-83 0.0 0.0 0.0 4.445676010678871 0.0 84-85 0.0 0.0 0.0 4.862104309687262 0.0 86-87 0.0 0.0 0.0 5.340865751334859 0.0 88-89 0.0 0.0 0.0 5.8582427536231885 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGTA 25 0.00501906 57.0 7 GGTATCA 1305 0.0 40.4023 1 GTATCAA 1940 0.0 32.074745 1 GCGTACA 60 0.003948117 31.666668 9 ATTAACG 65 0.005839897 29.23077 3 TCAACGC 2275 0.0 26.934065 4 CAACGCA 2320 0.0 26.411638 5 ATCAACG 2335 0.0 26.24197 3 AACGCAG 2395 0.0 26.17954 6 TATCAAC 2400 0.0 25.531248 2 TACATGG 1400 0.0 24.767857 2 GTACATG 1395 0.0 24.51613 1 CGCACGG 50 0.0016532751 23.75 70-71 ACGCAGA 2635 0.0 23.254269 7 CGCAGAG 2615 0.0 22.88719 8 ACATGGG 1450 0.0 22.603449 3 GAGTACT 1530 0.0 22.50817 12-13 CATGGGG 760 0.0 21.875002 4 AGAGTAC 2230 0.0 21.726458 10-11 GTATTAG 155 3.8988182E-5 21.451612 1 >>END_MODULE