FastQCFastQC Report
Fri 10 Feb 2017
SRR2031809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419520
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.5039092295957285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.4717295957284515No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT16390.390684591914569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11970.28532608695652173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.057.684611
GTATCAA18300.036.5976262
TCAACGC22150.030.236415
CAACGCA22600.029.6343636
ATCAACG22500.029.5549644
CGCAGAA1452.3928806E-829.4821689
ATAACGC650.0058404729.2301853
TATACGC650.0058404729.2301857
AACGCAG23100.029.1985477
TATCAAC22850.029.1022623
CGTGCGG350.00839112127.14231110-11
ACGCAGA24800.026.0478678
CGCAGAG24200.024.9271858
GAGTACT16950.023.81958212-13
GTATAGG1201.6865501E-423.79491
CAGAGTA25050.022.94365110-11
GCAGAGT26600.021.6067099
GATTGTC1100.002874689421.5904757
AGAGTAC22050.020.78756310-11
GTACTTT19550.020.65175814-15
Produced by FastQC (version 0.11.5)