FastQCFastQC Report
Fri 10 Feb 2017
SRR2031807_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031807_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880144
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.3501699721863695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.3033594502717737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.17042665745605265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.049.14741
GTATCAA27250.034.165141
ACCGCGT600.003950453431.6666689
CAACGCA34600.026.632955
TCAACGC34450.026.611034
ATCAACG34900.026.2679083
TATCAAC35050.026.1554932
AACGCAG35750.025.7762226
GTACATG23000.025.6086941
TACATGG24400.023.1659832
ACGCAGA39350.023.0559067
CGCAGAG39750.022.465418
ACATGGG24400.021.997953
CATGGGG14300.021.2587414
GTATAGG2951.3278623E-1020.9322031
GAGTACT26450.020.92154912-13
GTATAGC2751.2569217E-920.7272721
GTATTAC1404.8504956E-420.3571431
AGAGTAC36800.020.26494610-11
GTACTTT28650.019.39790714-15
Produced by FastQC (version 0.11.5)