##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031807_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 880144 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.471253567598026 34.0 31.0 34.0 31.0 34.0 2 32.605556590739695 34.0 31.0 34.0 31.0 34.0 3 32.678606000836226 34.0 31.0 34.0 31.0 34.0 4 35.884375738515516 37.0 37.0 37.0 35.0 37.0 5 35.90790938755477 37.0 37.0 37.0 35.0 37.0 6 35.98406624370558 37.0 37.0 37.0 35.0 37.0 7 36.001242978421715 37.0 37.0 37.0 35.0 37.0 8 35.99814575796688 37.0 37.0 37.0 35.0 37.0 9 37.81761961678998 39.0 39.0 39.0 35.0 39.0 10-11 37.849971141086 39.0 39.0 39.0 36.0 39.0 12-13 37.8222631751168 39.0 39.0 39.0 35.0 39.0 14-15 39.37538743660129 41.0 40.0 41.0 37.0 41.0 16-17 39.31222106837063 41.0 40.0 41.0 37.0 41.0 18-19 39.322394971731896 41.0 40.0 41.0 36.5 41.0 20-21 39.263785812321615 41.0 40.0 41.0 36.5 41.0 22-23 39.17731246250614 41.0 40.0 41.0 36.0 41.0 24-25 39.14349413277827 41.0 39.0 41.0 36.0 41.0 26-27 39.012603619407734 41.0 39.0 41.0 35.5 41.0 28-29 38.88756782980967 41.0 39.0 41.0 35.0 41.0 30-31 38.732016579105235 41.0 39.0 41.0 35.0 41.0 32-33 38.6006437582941 40.0 38.0 41.0 35.0 41.0 34-35 38.40470025359487 40.0 38.0 41.0 34.5 41.0 36-37 38.2894276391136 40.0 38.0 41.0 34.0 41.0 38-39 38.10165552455053 40.0 38.0 41.0 33.5 41.0 40-41 38.006233639040886 40.0 38.0 41.0 33.0 41.0 42-43 37.84342278081768 40.0 38.0 41.0 33.0 41.0 44-45 37.99028113581414 40.0 38.0 41.0 33.0 41.0 46-47 37.858520310312855 40.0 38.0 41.0 33.0 41.0 48-49 37.68443913723209 40.0 38.0 41.0 33.0 41.0 50-51 36.626215142067664 39.0 36.5 40.0 31.0 40.5 52-53 36.65100824410551 39.5 37.0 40.0 31.0 40.5 54-55 37.4313277145558 40.0 37.0 41.0 32.0 41.0 56-57 37.42584849751859 40.0 37.0 41.0 32.0 41.0 58-59 37.30018099311022 40.0 37.0 41.0 32.0 41.0 60-61 37.10056252158738 40.0 36.0 41.0 31.0 41.0 62-63 36.83241776345689 39.5 36.0 41.0 31.0 41.0 64-65 36.51260589176317 39.0 35.0 41.0 31.0 41.0 66-67 36.2378877774546 39.0 35.0 41.0 30.0 41.0 68-69 35.91676475667618 37.5 35.0 40.5 30.0 41.0 70-71 35.55354237488411 37.0 35.0 40.0 29.5 41.0 72-73 35.1500220418477 37.0 35.0 39.0 29.0 41.0 74-75 34.79005196876875 36.0 35.0 39.0 29.0 41.0 76-77 34.41990003908451 36.0 35.0 38.5 29.0 40.5 78-79 34.054092852987694 35.0 34.5 37.0 28.5 39.0 80-81 33.6979710138341 35.0 34.0 37.0 28.0 39.0 82-83 33.41403736206803 35.0 34.0 36.5 27.5 38.5 84-85 33.11653774836845 35.0 34.0 36.0 27.0 37.0 86-87 32.86371889145413 35.0 34.0 36.0 26.0 37.0 88-89 32.65901659273937 35.0 34.0 35.0 26.0 36.5 90-91 32.48916313694123 35.0 34.0 35.0 25.5 36.0 92-93 32.36727740006181 35.0 34.0 35.0 25.0 36.0 94-95 32.20317016306423 35.0 34.0 35.0 25.0 36.0 96-97 32.10092893890091 35.0 34.0 35.0 25.0 36.0 98-99 31.98844166409133 35.0 33.5 35.0 24.5 35.0 100-101 30.46608963987711 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13514763013473896 1101 2 0.04574103029541732 1101 3 0.06502833427995824 1101 4 0.07848081596866052 1101 5 0.1703452097098932 1101 6 0.13853267969343364 1101 7 0.09510257311111303 1101 8 0.29004858753717855 1101 9 0.22201730696190225 1101 10-11 0.23206632193637233 1101 12-13 0.22544043515794954 1101 14-15 0.3028440027301329 1101 16-17 0.318080691288408 1101 18-19 0.23738573584641642 1101 20-21 0.26282038012410425 1101 22-23 0.21248562081949984 1101 24-25 0.1839781589763234 1101 26-27 0.28044381010415265 1101 28-29 0.24962383770213137 1101 30-31 0.1361626616060363 1101 32-33 0.08418879166753612 1101 34-35 0.27133513992747993 1101 36-37 0.2853292876785858 1101 38-39 0.3438347010430789 1101 40-41 0.3955694633603031 1101 42-43 0.20827356343122716 1101 44-45 0.41956557595103305 1101 46-47 0.3964308518112105 1101 48-49 0.45993583807123883 1101 50-51 0.6257912264398584 1101 52-53 0.4881045892319946 1101 54-55 0.2884827463144646 1101 56-57 0.32387323748014296 1101 58-59 0.3039377734568589 1101 60-61 0.24251456620144296 1101 62-63 0.2623044699586714 1101 64-65 0.3296223024853191 1101 66-67 0.3105239402856057 1101 68-69 0.3298656295562026 1101 70-71 0.17849467739166158 1101 72-73 0.22460094787223994 1101 74-75 0.4177141312497952 1101 76-77 0.3985698702584415 1101 78-79 0.5901068526579536 1101 80-81 0.5196769424584531 1101 82-83 0.43026625210769964 1101 84-85 0.3468889120184002 1101 86-87 0.41685352074338766 1101 88-89 0.28834430770113073 1101 90-91 0.31793029609480783 1101 92-93 0.38486081385278226 1101 94-95 0.31586094506349127 1101 96-97 0.15007022400152437 1101 98-99 0.2256964288311707 1101 100-101 0.1772741253185366 1102 1 0.19013495316902151 1102 2 0.17362090620287773 1102 3 0.28359969536089125 1102 4 0.27596983188966817 1102 5 0.25703282092861457 1102 6 0.16932353338077633 1102 7 0.26415109203207265 1102 8 0.3013830547109251 1102 9 0.34557047935288665 1102 10-11 0.24711726601599082 1102 12-13 0.27431564972150113 1102 14-15 0.24484885304745063 1102 16-17 0.31906099081124495 1102 18-19 0.2609975687309358 1102 20-21 0.3219788973128104 1102 22-23 0.3173383003048613 1102 24-25 0.348282658118066 1102 26-27 0.22781471764891137 1102 28-29 0.4233907862541173 1102 30-31 0.3091520633477458 1102 32-33 0.36104269948001644 1102 34-35 0.31700568054888834 1102 36-37 0.4556966834676288 1102 38-39 0.5748684642909083 1102 40-41 0.31607071530909536 1102 42-43 0.2674694816057439 1102 44-45 0.2949199010797656 1102 46-47 0.36318728084381746 1102 48-49 0.46686683128395146 1102 50-51 0.4891437720690561 1102 52-53 0.6156478562671523 1102 54-55 0.5345162733455311 1102 56-57 0.2644017330943882 1102 58-59 0.3265496220891677 1102 60-61 0.4306513671967025 1102 62-63 0.5959232318679355 1102 64-65 0.5490479808210935 1102 66-67 0.4809641695733404 1102 68-69 0.49135914934329605 1102 70-71 0.6453436246523339 1102 72-73 0.43070400136746656 1102 74-75 0.38608667656404094 1102 76-77 0.4655786575216325 1102 78-79 0.4020842230930697 1102 80-81 0.38788374235137013 1102 82-83 0.341501901939985 1102 84-85 0.4577316546523704 1102 86-87 0.3998262298345878 1102 88-89 0.5448284575570383 1102 90-91 0.46217211905823063 1102 92-93 0.3889611429814579 1102 94-95 0.40812327164103124 1102 96-97 0.3089535486735713 1102 98-99 0.4676315952064982 1102 100-101 0.3451828984087264 1103 1 0.15633402164007038 1103 2 0.21430378294032693 1103 3 0.279429819686996 1103 4 0.23205410958798467 1103 5 0.24135269476879273 1103 6 0.25977902903486694 1103 7 0.22254585493228518 1103 8 0.26716403538353717 1103 9 0.42365937802161113 1103 10-11 0.28751958383606535 1103 12-13 0.3757798738752456 1103 14-15 0.3605418152361679 1103 16-17 0.2915387111325103 1103 18-19 0.34925351914719016 1103 20-21 0.4648869913458924 1103 22-23 0.4290649295636726 1103 24-25 0.454088210617428 1103 26-27 0.5131266331511739 1103 28-29 0.488587727611808 1103 30-31 0.5120383361316954 1103 32-33 0.437385898218416 1103 34-35 0.38878383729514354 1103 36-37 0.5297183946936528 1103 38-39 0.6666341517519427 1103 40-41 0.5243279493282316 1103 42-43 0.6077049310501295 1103 44-45 0.7077403433726488 1103 46-47 0.5323881463589046 1103 48-49 0.5038336776550167 1103 50-51 0.3285173738587659 1103 52-53 0.5045456081283817 1103 54-55 0.48920417447143194 1103 56-57 0.43151399333570595 1103 58-59 0.4786868209009185 1103 60-61 0.6554789976657673 1103 62-63 0.6087069997925312 1103 64-65 0.5589793997524026 1103 66-67 0.6680080321178039 1103 68-69 0.5097623473482074 1103 70-71 0.6667110284738058 1103 72-73 0.6407344410931373 1103 74-75 0.5470954050636934 1103 76-77 0.47255687294688187 1103 78-79 0.503220211650671 1103 80-81 0.6187195512045562 1103 82-83 0.6153686639600053 1103 84-85 0.6229744389704166 1103 86-87 0.7026831631239077 1103 88-89 0.6545600011812027 1103 90-91 0.6789983192269275 1103 92-93 0.7453544022325644 1103 94-95 0.7181335404641516 1103 96-97 0.5768781461723833 1103 98-99 0.632458125444515 1103 100-101 0.7195337620900979 1104 1 0.1449761198566506 1104 2 0.08395310750820784 1104 3 0.05161780780900216 1104 4 0.2408664819253019 1104 5 0.045633578409088216 1104 6 0.1743862141908039 1104 7 0.06035460551159133 1104 8 0.14501295143084292 1104 9 0.19357475590314266 1104 10-11 0.088154678842848 1104 12-13 0.18500248325747748 1104 14-15 0.2194271376756305 1104 16-17 0.23769123692252236 1104 18-19 0.17247053282748226 1104 20-21 0.2768113292164358 1104 22-23 0.20488669100825518 1104 24-25 0.2771885041343083 1104 26-27 0.3708275892266286 1104 28-29 0.4950596890254957 1104 30-31 0.2507233461730607 1104 32-33 0.3399709065433072 1104 34-35 0.44544718544735673 1104 36-37 0.3766399003837648 1104 38-39 0.4008837955837876 1104 40-41 0.46166209512545464 1104 42-43 0.45955786364422835 1104 44-45 0.37981676075022364 1104 46-47 0.4766765824803727 1104 48-49 0.4029788741053508 1104 50-51 0.47848733558141987 1104 52-53 0.45333635290601393 1104 54-55 0.5102318268513244 1104 56-57 0.5750875184562858 1104 58-59 0.3408729209026333 1104 60-61 0.5133121221615582 1104 62-63 0.4469654662955662 1104 64-65 0.4259853559136815 1104 66-67 0.3848972681156795 1104 68-69 0.45904743634734047 1104 70-71 0.38786140867257046 1104 72-73 0.47817929939829185 1104 74-75 0.503987044123754 1104 76-77 0.3864362735545086 1104 78-79 0.562994667099602 1104 80-81 0.6610623651903822 1104 82-83 0.7586804269494181 1104 84-85 0.5793914732483785 1104 86-87 0.4376897715449246 1104 88-89 0.5051865138230269 1104 90-91 0.48280717279461527 1104 92-93 0.4665382536546687 1104 94-95 0.5048557636399664 1104 96-97 0.6520768279211282 1104 98-99 0.5906680157618922 1104 100-101 0.6345365942705321 1105 1 0.17846907300461368 1105 2 0.05835045201492761 1105 3 0.21532788018208038 1105 4 0.19961927073740782 1105 5 0.28479122111463795 1105 6 0.2676277661307296 1105 7 0.2283635297103217 1105 8 0.25738167320262306 1105 9 0.35989678887914067 1105 10-11 0.34124277809734593 1105 12-13 0.45161292021673205 1105 14-15 0.5090554774481646 1105 16-17 0.4925206432456477 1105 18-19 0.38425700504819105 1105 20-21 0.3808272186434394 1105 22-23 0.4394461947357442 1105 24-25 0.35081314362933114 1105 26-27 0.3137725748064284 1105 28-29 0.42101332672478975 1105 30-31 0.5382430719172007 1105 32-33 0.4409667165231781 1105 34-35 0.6215504667228586 1105 36-37 0.6060571066742355 1105 38-39 0.5102188339469578 1105 40-41 0.5031949895042942 1105 42-43 0.6117317766877122 1105 44-45 0.5960109164883747 1105 46-47 0.7201874928574696 1105 48-49 0.840863186016982 1105 50-51 0.5452555784937587 1105 52-53 0.512377545701348 1105 54-55 0.51345596572056 1105 56-57 0.5635649630881687 1105 58-59 0.5053720230411827 1105 60-61 0.40978597534760297 1105 62-63 0.48040400359762714 1105 64-65 0.5469517813367659 1105 66-67 0.41369685455524774 1105 68-69 0.4646522684744525 1105 70-71 0.5710251459528664 1105 72-73 0.44400614817665485 1105 74-75 0.27578059040443037 1105 76-77 0.39321104636835 1105 78-79 0.4468712519611131 1105 80-81 0.524464988034147 1105 82-83 0.4948724060537373 1105 84-85 0.48127970310090973 1105 86-87 0.3692210334483903 1105 88-89 0.2766807729512806 1105 90-91 0.3729143720110315 1105 92-93 0.4208153391419671 1105 94-95 0.4957002975682485 1105 96-97 0.3879423119500771 1105 98-99 0.3230100737691828 1105 100-101 0.229097730473395 1106 1 0.19016147977520603 1106 2 0.12427478704536554 1106 3 0.2232185080299942 1106 4 0.19559421514607322 1106 5 0.2752632310299461 1106 6 0.25491162823992397 1106 7 0.28201676129835107 1106 8 0.3052692025171737 1106 9 0.2836971704240341 1106 10-11 0.34730162592898495 1106 12-13 0.3076761728261772 1106 14-15 0.2693163314282643 1106 16-17 0.40981182639934133 1106 18-19 0.43711433885136586 1106 20-21 0.4113105594699604 1106 22-23 0.40726017944964354 1106 24-25 0.46505941020041064 1106 26-27 0.338977776697142 1106 28-29 0.3149234935595473 1106 30-31 0.42680750925999433 1106 32-33 0.4867953919305492 1106 34-35 0.49485994337450023 1106 36-37 0.4656806348708926 1106 38-39 0.5831414495951677 1106 40-41 0.5614451383554027 1106 42-43 0.4472900291149031 1106 44-45 0.5698184368111114 1106 46-47 0.4774075312549826 1106 48-49 0.3656446179039321 1106 50-51 0.3582780762761715 1106 52-53 0.4230182737959396 1106 54-55 0.7252657826033158 1106 56-57 0.5596196491642047 1106 58-59 0.6174834912599465 1106 60-61 0.6574704292159126 1106 62-63 0.6890150404519488 1106 64-65 0.7465087214439379 1106 66-67 0.5157836560182432 1106 68-69 0.6151787153680175 1106 70-71 0.5290741939133099 1106 72-73 0.5510817402395531 1106 74-75 0.5110435708289955 1106 76-77 0.631214102372823 1106 78-79 0.5779954120155537 1106 80-81 0.5907857530681113 1106 82-83 0.659960440854519 1106 84-85 0.6162679165230287 1106 86-87 0.5886625076212084 1106 88-89 0.4908236031881046 1106 90-91 0.5328655245439293 1106 92-93 0.5931894840075103 1106 94-95 0.5853641066985915 1106 96-97 0.7097705681441084 1106 98-99 0.6024066495860438 1106 100-101 0.8401892647942084 1107 1 0.014415967406925745 1107 2 0.14014387025832065 1107 3 0.13930147462611586 1107 4 0.1246235653213219 1107 5 0.21107162063364626 1107 6 0.16227548754720544 1107 7 0.17938073258646625 1107 8 0.11492418958339101 1107 9 0.13181757221124712 1107 10-11 0.14304159524623827 1107 12-13 0.08881874121846778 1107 14-15 0.018810318057752795 1107 16-17 0.19679559477820163 1107 18-19 0.2700333431178379 1107 20-21 0.1892028843039455 1107 22-23 0.2326435738125312 1107 24-25 0.22255232741298414 1107 26-27 0.2186536761831519 1107 28-29 0.35628781198823845 1107 30-31 0.16322773411749836 1107 32-33 0.20269093061432386 1107 34-35 0.14733024045964527 1107 36-37 0.22960615082255487 1107 38-39 0.3458589723552663 1107 40-41 0.4431198956369613 1107 42-43 0.4006591123278511 1107 44-45 0.38638614042024955 1107 46-47 0.320766648174164 1107 48-49 0.42760151088385356 1107 50-51 0.46953592893179774 1107 52-53 0.3124707995235312 1107 54-55 0.43396935255213975 1107 56-57 0.41991726621216685 1107 58-59 0.3929766268351358 1107 60-61 0.3436616532783532 1107 62-63 0.4770542281430181 1107 64-65 0.5364361858620299 1107 66-67 0.504913816934689 1107 68-69 0.4814134159872694 1107 70-71 0.4383102327034649 1107 72-73 0.5496651827541896 1107 74-75 0.5320322775624007 1107 76-77 0.4124630525525532 1107 78-79 0.345101883387656 1107 80-81 0.3470986722963474 1107 82-83 0.2622409304347002 1107 84-85 0.1624866038837567 1107 86-87 0.3119312840066897 1107 88-89 0.3922909719745675 1107 90-91 0.24460447483563286 1107 92-93 0.17050244802807413 1107 94-95 0.2579398617293265 1107 96-97 0.2285273569818571 1107 98-99 0.21201348489583438 1107 100-101 0.2582640903840705 1108 1 0.3149010284740612 1108 2 0.24276904603370753 1108 3 0.24914726628685457 1108 4 0.2644420229219264 1108 5 0.3270043756857106 1108 6 0.3611979251765689 1108 7 0.34718500175586087 1108 8 0.30392815539211426 1108 9 0.39205599473577735 1108 10-11 0.3686364665549533 1108 12-13 0.26729790887263505 1108 14-15 0.3338057360476725 1108 16-17 0.3507531084847315 1108 18-19 0.22860367697256834 1108 20-21 0.3402156665320675 1108 22-23 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-0.665598552492348 2316 52-53 -0.6738415563011486 2316 54-55 -0.6764931277186079 2316 56-57 -0.7029035048981314 2316 58-59 -0.7534964844682079 2316 60-61 -0.8332120567319663 2316 62-63 -1.0335953006313474 2316 64-65 -1.0134475253623947 2316 66-67 -0.8038741429086826 2316 68-69 -0.5493907787556438 2316 70-71 -0.5982571806395782 2316 72-73 -0.41046654795666626 2316 74-75 -0.8749186800652424 2316 76-77 -1.0980470435137377 2316 78-79 -0.5807336694867402 2316 80-81 -0.6716256227622353 2316 82-83 -0.7060134669124736 2316 84-85 -0.41390022987452113 2316 86-87 -0.4378728462367434 2316 88-89 -0.44406956875874926 2316 90-91 -0.3500627457677723 2316 92-93 -0.5464335039843142 2316 94-95 -0.7152634482324274 2316 96-97 -0.6799602059999899 2316 98-99 -0.6422399715176894 2316 100-101 -0.3728121404178495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 15.0 7 339.0 8 772.0 9 597.0 10 590.0 11 781.0 12 1052.0 13 1092.0 14 1189.0 15 1293.0 16 1621.0 17 2152.0 18 2625.0 19 3541.0 20 4560.0 21 5607.0 22 6439.0 23 6702.0 24 6186.0 25 5544.0 26 5537.0 27 5749.0 28 6566.0 29 7667.0 30 8973.0 31 10811.0 32 13755.0 33 18582.0 34 26914.0 35 44049.0 36 84228.0 37 191201.0 38 300229.0 39 103093.0 40 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01955134614336 19.726999218309732 13.78444890836045 23.46900052718646 2 15.205693613772292 21.707697831263975 39.75281317602574 23.333795378937992 3 17.258653129487904 26.52293261102729 31.767642567579852 24.45077169190496 4 12.220045810685525 18.232471050191787 39.45308949444637 30.094393644676327 5 12.851419767674379 38.22033667218091 35.8265238415532 13.101719718591504 6 29.47813085131524 38.7502499590976 18.406987947426785 13.364631242160375 7 25.600924394190045 33.835372393608324 23.470591175989384 17.093112036212258 8 24.970913850460832 34.83998073042593 21.741783162755183 18.44732225635805 9 26.022105473649766 15.147634932465598 21.302764093148394 37.527495500736244 10-11 23.33084131687542 26.437946517842537 30.517051755167333 19.714160410114708 12-13 24.71067234452544 24.80446381501209 29.48341407769638 21.001449762766093 14-15 22.050425839408096 26.243603319456817 27.342514406733443 24.36345643440164 16-17 21.238001963315096 29.08347952153284 27.99201039829846 21.686508116853606 18-19 21.178863913177846 28.173571597374973 29.883291824974094 20.764272664473086 20-21 22.1255271864604 27.50021587376611 29.690652893162937 20.683604046610554 22-23 21.852049210129252 27.614458543147485 29.280208693122944 21.25328355360032 24-25 21.656979799316815 27.642935498586308 29.43412068600177 21.2659640160951 26-27 21.644355923576143 27.810506008107765 29.34639104510171 21.198747023214384 28-29 21.595102619571342 27.75290179788762 29.432058844916288 21.219936737624753 30-31 21.74127188278282 27.670528913450525 29.47955107346071 21.108648130305948 32-33 21.58453616680907 27.881857968696032 29.297024123325272 21.236581741169626 34-35 21.864660782781 27.933497245905215 29.18528104491992 21.01656092639386 36-37 21.848129397007764 27.69836526750168 29.092398516606373 21.36110681888418 38-39 21.683326819247757 27.809540257048848 29.16073960624625 21.346393317457142 40-41 21.773255285498735 27.77886345870676 29.2061867149012 21.241694540893306 42-43 21.67764593066589 27.898559781126725 28.933106400770782 21.490687887436604 44-45 21.735420567543493 28.05631805704521 28.740865131160355 21.467396244250942 46-47 21.790922848988348 27.89611699903652 28.651504753767565 21.661455398207565 48-49 21.838642313076043 28.04870566634551 28.42989329018888 21.682758730389573 50-51 21.741215073897 28.194363649584613 28.399841389590797 21.664579886927594 52-53 21.8388127397335 28.229812394335475 28.241515024814124 21.689859841116906 54-55 21.762007126106635 28.15351806068098 28.322808540420656 21.761666272791725 56-57 21.717128106309875 28.273441618644224 28.221915959206672 21.787514315839225 58-59 21.740192513952262 28.120455289134505 28.384275754876477 21.75507644203676 60-61 21.828075860313767 28.03552600483557 28.413191477758186 21.723206657092476 62-63 21.714969368648767 28.13301005290044 28.352690014361286 21.799330564089512 64-65 21.66475031358505 28.050580359577527 28.42341707720555 21.861252249631878 66-67 21.66469350469923 28.07966650911669 28.325080895853404 21.93055909033067 68-69 21.483586776709267 28.163914086785795 28.279008889454456 22.073490247050483 70-71 21.57675334945191 27.94065516551837 28.315025723063496 22.167565761966223 72-73 21.55255276409315 27.939121325601263 28.29002981330328 22.21829609700231 74-75 21.472906706175355 28.07279263393263 28.24623016233707 22.208070497554946 76-77 21.439048610227417 28.036775800323582 28.238788198294824 22.285387391154174 78-79 21.458590866949045 27.8306731625734 28.298664763947716 22.41207120652984 80-81 21.41859741133269 28.00587176643822 28.160619171408314 22.414911650820773 82-83 21.421437855623626 27.900775327673657 28.221461488120127 22.456325328582597 84-85 21.303502608664036 27.89066334599793 28.31053782108382 22.49529622425421 86-87 21.405872220909306 27.71035194240942 28.29599474631424 22.58778109036703 88-89 21.33224790488829 27.660473740660617 28.26963542329437 22.73764293115672 90-91 21.41473440709702 27.506919322292717 28.393706029922377 22.684640240687887 92-93 21.38201248886546 27.338708211383594 28.49022432692832 22.78905497282263 94-95 21.287482502863167 27.29877156465306 28.5318084313476 22.881937501136175 96-97 21.16358232289262 27.273037139377195 28.60895489828937 22.954425639440817 98-99 21.27310985475104 27.213842280354122 28.397682651929685 23.11536521296515 100-101 21.389511261793526 27.02540720609355 28.647698558417712 22.937382973695215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 411.0 1 282.5 2 194.0 3 239.0 4 206.5 5 201.0 6 300.5 7 541.5 8 923.0 9 1159.0 10 1000.0 11 798.0 12 795.0 13 857.0 14 934.5 15 1004.5 16 1100.5 17 1178.0 18 1314.0 19 1502.0 20 1727.5 21 2044.0 22 2409.0 23 2928.0 24 3651.0 25 4553.0 26 5715.0 27 7167.5 28 8742.0 29 10514.0 30 12216.5 31 14113.0 32 16363.0 33 18270.0 34 20028.5 35 21862.0 36 23660.5 37 25337.5 38 26501.5 39 27227.5 40 28111.0 41 29296.5 42 30240.5 43 31362.0 44 32569.0 45 33978.0 46 35071.0 47 35391.5 48 36042.5 49 36103.5 50 34451.0 51 32227.0 52 30403.0 53 28400.0 54 25925.0 55 23289.0 56 20510.0 57 17403.5 58 14903.5 59 12828.0 60 10463.5 61 8111.5 62 6153.0 63 4506.5 64 3184.0 65 2351.5 66 1778.0 67 1349.0 68 997.5 69 709.5 70 547.5 71 406.5 72 273.0 73 235.0 74 203.0 75 149.5 76 103.5 77 72.5 78 57.0 79 41.5 80 33.0 81 25.0 82 17.5 83 17.5 84 15.5 85 11.5 86 9.5 87 8.0 88 6.5 89 7.5 90 7.0 91 4.5 92 3.0 93 2.5 94 2.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.6808885818684215E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 880144.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.074418971479474 #Duplication Level Percentage of deduplicated Percentage of total 1 72.95242582711646 46.014318716053225 2 15.674487512634835 19.773183850703052 3 5.571482675535966 10.542540977072848 4 2.4290766337138257 6.128503892347873 5 1.2558022488941076 3.960449859603655 6 0.6902601898174192 2.6122656247126104 7 0.40597000528198035 1.7924425542106555 8 0.2746306097460496 1.3857732913212164 9 0.17265878742166982 0.9801317427247812 >10 0.5594379781722925 5.14893882180054 >50 0.009601883053969936 0.40244106699262133 >100 0.0036223031653281135 0.43056050179238947 >500 0.0 0.0 >1k 5.433454461643771E-4 0.8284491006645371 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3082 0.3501699721863695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2670 0.3033594502717737 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1500 0.17042665745605265 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.1361777163736843E-4 0.0 5 0.0 0.0 0.0 1.1361777163736843E-4 0.0 6 0.0 0.0 0.0 1.1361777163736843E-4 0.0 7 0.0 0.0 0.0 1.1361777163736843E-4 0.0 8 0.0 0.0 0.0 1.1361777163736843E-4 0.0 9 0.0 0.0 0.0 1.1361777163736843E-4 0.0 10-11 0.0 0.0 0.0 1.1361777163736843E-4 0.0 12-13 0.0 0.0 0.0 1.1361777163736843E-4 5.6808885818684215E-5 14-15 0.0 0.0 0.0 2.2723554327473686E-4 1.7042665745605265E-4 16-17 0.0 0.0 0.0 2.2723554327473686E-4 2.2723554327473686E-4 18-19 0.0 0.0 0.0 2.8404442909342107E-4 2.2723554327473686E-4 20-21 0.0 0.0 0.0 6.817066298242106E-4 2.2723554327473686E-4 22-23 0.0 0.0 0.0 0.001079368830555 2.2723554327473686E-4 24-25 0.0 0.0 0.0 0.0019883110036539474 2.2723554327473686E-4 26-27 0.0 0.0 0.0 0.00431747532222 2.2723554327473686E-4 28-29 0.0 0.0 0.0 0.011532203821192896 2.2723554327473686E-4 30-31 0.0 0.0 0.0 0.026359323019869478 2.2723554327473686E-4 32-33 0.0 0.0 0.0 0.04737861077278263 2.2723554327473686E-4 34-35 0.0 0.0 0.0 0.08044138231925685 2.2723554327473686E-4 36-37 0.0 0.0 0.0 0.1382160191968587 2.2723554327473686E-4 38-39 0.0 0.0 0.0 0.21212437964696687 2.2723554327473686E-4 40-41 0.0 0.0 0.0 0.29461088185569634 2.2723554327473686E-4 42-43 0.0 0.0 0.0 0.3672694468177935 2.2723554327473686E-4 44-45 0.0 0.0 0.0 0.45907260630078717 2.2723554327473686E-4 46-47 0.0 0.0 0.0 0.5796778708938537 2.2723554327473686E-4 48-49 0.0 0.0 0.0 0.7103951171626461 2.2723554327473686E-4 50-51 0.0 0.0 0.0 0.8303186751258884 2.2723554327473686E-4 52-53 0.0 0.0 0.0 0.944277300078169 2.2723554327473686E-4 54-55 0.0 0.0 0.0 1.0764147684924286 2.2723554327473686E-4 56-57 0.0 0.0 0.0 1.2434897016851787 2.2723554327473686E-4 58-59 0.0 0.0 0.0 1.4355037357523315 2.2723554327473686E-4 60-61 0.0 0.0 0.0 1.6457534221672816 2.2723554327473686E-4 62-63 0.0 0.0 0.0 1.8428802559581159 2.2723554327473686E-4 64-65 0.0 0.0 0.0 2.0522210001999674 2.2723554327473686E-4 66-67 0.0 0.0 0.0 2.3033730844043703 2.2723554327473686E-4 68-69 0.0 0.0 0.0 2.606164445817957 2.2723554327473686E-4 70-71 0.0 0.0 0.0 2.925373575233144 2.2723554327473686E-4 72-73 0.0 0.0 0.0 3.2612845170790234 2.2723554327473686E-4 74-75 0.0 0.0 0.0 3.5969114144958096 2.2723554327473686E-4 76-77 0.0 0.0 0.0 3.967134923376175 2.2723554327473686E-4 78-79 0.0 0.0 0.0 4.392803904815576 2.2723554327473686E-4 80-81 0.0 0.0 0.0 4.860170608445891 2.2723554327473686E-4 82-83 0.0 0.0 0.0 5.332706920685705 2.2723554327473686E-4 84-85 0.0 0.0 0.0 5.849610972749914 2.2723554327473686E-4 86-87 0.0 0.0 0.0 6.404236124997728 3.408533149121053E-4 88-89 5.6808885818684215E-5 0.0 0.0 7.017885709611154 4.544710865494737E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1730 0.0 49.1474 1 GTATCAA 2725 0.0 34.16514 1 ACCGCGT 60 0.0039504534 31.666668 9 CAACGCA 3460 0.0 26.63295 5 TCAACGC 3445 0.0 26.61103 4 ATCAACG 3490 0.0 26.267908 3 TATCAAC 3505 0.0 26.155493 2 AACGCAG 3575 0.0 25.776222 6 GTACATG 2300 0.0 25.608694 1 TACATGG 2440 0.0 23.165983 2 ACGCAGA 3935 0.0 23.055906 7 CGCAGAG 3975 0.0 22.46541 8 ACATGGG 2440 0.0 21.99795 3 CATGGGG 1430 0.0 21.258741 4 GTATAGG 295 1.3278623E-10 20.932203 1 GAGTACT 2645 0.0 20.921549 12-13 GTATAGC 275 1.2569217E-9 20.727272 1 GTATTAC 140 4.8504956E-4 20.357143 1 AGAGTAC 3680 0.0 20.264946 10-11 GTACTTT 2865 0.0 19.397907 14-15 >>END_MODULE