FastQCFastQC Report
Fri 10 Feb 2017
SRR2031807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880144
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.41368230653165844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.36141813157846897No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22180.2520042174916832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.21598738388263738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.048.79551
GTATCAA33150.039.1947631
CAACGCA38950.032.804195
TCAACGC39050.032.7201844
ATCAACG39300.032.5120433
AACGCAG39300.032.5120436
TATCAAC40000.032.180582
ACGCAGA42950.029.5279057
CGCAGAG42750.029.3327228
GTACATG21850.026.5740241
GCAGAGT47400.026.0543089
GAGTACT30750.025.101112-13
TACATGG22950.024.6291122
AGAGTAC40100.023.3941510-11
GTACTTT34300.022.91861714-15
ACATGGG23450.022.8886473
CAGAGTA46350.022.1354610-11
AGTACTT33200.020.81658412-13
GCACCGT1200.00476320619.7912526
CATGGGG12250.018.9996014
Produced by FastQC (version 0.11.5)