FastQCFastQC Report
Fri 10 Feb 2017
SRR2031806_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031806_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65692
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8601.309139621262863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8531.2984838336479327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7521.1447360409182243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.7519941545393655No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.5495341898556901No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.3957863971259819No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.26335017962613405No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.24356085976983496No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.23290507215490472No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.18571515557449916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.15679230347683126No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.13700298362053218No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.10351336540217987No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC250.005000266757.0000043
ATACTTA352.7685764E-454.285716
GGTATCA5100.049.3627431
CCCCGCG205.370653E-447.50000490-91
CGCAGAA502.7977392E-547.5000049
GGATGAC500.001604739638.0000048
AATACCT500.001604739638.0000045
GTATCAA7950.034.0566021
ATTAGCC352.0434771E-433.9285726-27
CGAAACC300.0039383331.66666874-75
GACACAA300.0039383331.66666848-49
ACCCTAC650.005804545729.230771
CTACACG650.005804545729.230774
CGGAGGT650.005804545729.230779
TACACGG650.005804545729.230775
AACGCAG9400.027.7925536
TATCAAC9750.027.2820512
CAACGCA9450.027.1428575
TCAACGC9450.027.1428574
ACACGGA700.0083350727.1428556
Produced by FastQC (version 0.11.5)