##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031806_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65692 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.871963100529744 34.0 31.0 34.0 30.0 34.0 2 31.9599494611216 34.0 31.0 34.0 30.0 34.0 3 31.962430737380505 34.0 31.0 34.0 30.0 34.0 4 35.1519515313889 37.0 35.0 37.0 33.0 37.0 5 35.22069658405894 37.0 35.0 37.0 33.0 37.0 6 35.34329903184558 37.0 37.0 37.0 35.0 37.0 7 35.367487669731474 37.0 37.0 37.0 35.0 37.0 8 35.37357669122572 37.0 37.0 37.0 35.0 37.0 9 37.11887292212141 39.0 39.0 39.0 35.0 39.0 10-11 37.14204926018389 39.0 39.0 39.0 35.0 39.0 12-13 37.100438409547586 39.0 39.0 39.0 35.0 39.0 14-15 38.51360135176277 41.0 39.0 41.0 34.5 41.0 16-17 38.43558576386775 41.0 39.0 41.0 34.5 41.0 18-19 38.48494489435548 41.0 39.0 41.0 35.0 41.0 20-21 38.45764324423065 41.0 39.0 41.0 35.0 41.0 22-23 38.32680539487305 41.0 39.0 41.0 34.0 41.0 24-25 38.22969311331669 41.0 39.0 41.0 34.0 41.0 26-27 38.04333861048529 41.0 38.0 41.0 34.0 41.0 28-29 37.833084698288985 40.5 38.0 41.0 33.5 41.0 30-31 37.53620684406016 40.0 37.0 41.0 33.0 41.0 32-33 37.24570723984655 40.0 35.5 41.0 33.0 41.0 34-35 36.9875403397674 40.0 35.0 41.0 31.5 41.0 36-37 36.75981854715947 40.0 35.0 41.0 30.5 41.0 38-39 36.49231261036351 40.0 35.0 41.0 30.0 41.0 40-41 36.28144218474091 40.0 35.0 41.0 29.0 41.0 42-43 36.00551817572916 40.0 35.0 41.0 26.0 41.0 44-45 35.88993332521464 40.0 35.0 41.0 23.5 41.0 46-47 35.48341502770505 40.0 35.0 41.0 19.5 41.0 48-49 35.06091304877306 40.0 35.0 41.0 14.5 41.0 50-51 34.01609023929855 38.5 34.0 40.0 12.5 40.5 52-53 33.95882299214516 38.5 35.0 39.5 10.5 40.5 54-55 34.58082414905924 40.0 35.0 41.0 11.0 41.0 56-57 34.514164586250985 40.0 35.0 41.0 10.0 41.0 58-59 34.328517932168296 39.0 35.0 41.0 10.0 41.0 60-61 34.094410278268285 39.0 35.0 41.0 10.0 41.0 62-63 33.77131157522986 39.0 35.0 41.0 10.0 41.0 64-65 33.43491597150338 38.0 34.5 41.0 9.0 41.0 66-67 33.114131096632775 37.0 34.0 40.5 8.0 41.0 68-69 32.716183096876335 37.0 34.0 40.0 8.0 41.0 70-71 32.3303141935091 36.0 33.5 39.0 8.0 41.0 72-73 31.881987152164648 36.0 33.0 39.0 7.0 41.0 74-75 31.46142604883395 35.0 33.0 38.0 7.0 40.5 76-77 31.06987152164647 35.0 32.0 37.0 7.0 39.5 78-79 30.716411435182366 35.0 32.0 37.0 7.0 39.0 80-81 30.35505084332948 35.0 31.0 36.0 7.0 39.0 82-83 30.04316355111734 35.0 31.0 36.0 7.0 37.0 84-85 29.742069049503744 35.0 30.5 35.5 7.0 37.0 86-87 29.49850057845704 35.0 30.0 35.0 7.0 36.5 88-89 29.274203860439627 35.0 29.0 35.0 7.0 36.0 90-91 29.05866772209706 35.0 29.0 35.0 7.0 36.0 92-93 28.909631309748523 35.0 29.0 35.0 7.0 36.0 94-95 28.675516044571637 35.0 27.0 35.0 7.0 36.0 96-97 28.553179991475368 35.0 27.0 35.0 7.0 35.0 98-99 28.421550569323507 35.0 26.0 35.0 7.0 35.0 100-101 27.131469585337637 33.5 21.5 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4707719143379663 1101 2 0.6427424165303393 1101 3 0.3036011109226493 1101 4 0.45664546750084156 1101 5 0.5516152055364856 1101 6 0.4661446809923433 1101 7 0.4978551135522764 1101 8 0.6722622545731554 1101 9 0.5116151785001151 1101 10-11 0.46548546959159864 1101 12-13 -0.016601938210548894 1101 14-15 0.05187094678483106 1101 16-17 0.2632244841878091 1101 18-19 0.6032481163181558 1101 20-21 0.4016007089396467 1101 22-23 0.6107474537303617 1101 24-25 0.39617500946406636 1101 26-27 0.7772233086481961 1101 28-29 1.0226005946671748 1101 30-31 1.118442454794959 1101 32-33 0.868592887426388 1101 34-35 1.086526260332512 1101 36-37 1.1091031101676307 1101 38-39 1.4911144156079743 1101 40-41 1.9355913439242087 1101 42-43 1.5689794695681556 1101 44-45 1.9227016288907208 1101 46-47 1.9898819588080556 1101 48-49 2.0593364552123035 1101 50-51 1.7522065250848797 1101 52-53 2.1317992760953004 1101 54-55 2.1300295847791872 1101 56-57 2.154240047501496 1101 58-59 2.622011570823311 1101 60-61 2.455731590584719 1101 62-63 2.846620302229468 1101 64-65 2.8156101951764185 1101 66-67 2.392432659898901 1101 68-69 2.5445443133895225 1101 70-71 2.474117805804063 1101 72-73 2.5180036975670674 1101 74-75 2.592591698044547 1101 76-77 2.7255879717909473 1101 78-79 2.0916684697569146 1101 80-81 1.9321982904658945 1101 82-83 2.1527693951143085 1101 84-85 1.7042186688737537 1101 86-87 2.0419940137446417 1101 88-89 2.6345558188714655 1101 90-91 2.266190620955765 1101 92-93 1.6590321489069275 1101 94-95 2.264920674256196 1101 96-97 2.3832519128825105 1101 98-99 2.0501829166387466 1101 100-101 2.5549914186888607 1102 1 0.8970539656200174 1102 2 0.8382552370431569 1102 3 0.9286011109226493 1102 4 1.112094185449564 1102 5 1.1750126414339164 1102 6 1.1280036553513142 1102 7 0.9577910109881742 1102 8 0.7571981520090532 1102 9 1.2055254349103706 1102 10-11 0.8805495721557008 1102 12-13 0.9377249848663709 1102 14-15 0.40283248524637116 1102 16-17 0.7071347405980575 1102 18-19 1.1529276034976448 1102 20-21 1.2076904525293983 1102 22-23 1.1612282229611282 1102 24-25 1.3072327017717598 1102 26-27 0.6217745906994736 1102 28-29 0.4889467485133281 1102 30-31 0.9686027112052074 1102 32-33 0.8750031438366435 1102 34-35 1.2363660039222637 1102 36-37 1.8558979819624923 1102 38-39 1.479095184838748 1102 40-41 1.1679631387960043 1102 42-43 0.580998700337382 1102 44-45 1.6919323981214944 1102 46-47 1.6244973434234424 1102 48-49 1.3862595321353837 1102 50-51 1.937302678931033 1102 52-53 1.868177481223512 1102 54-55 0.9978180463176543 1102 56-57 1.1710669705784156 1102 58-59 0.8463705451822889 1102 60-61 1.136020052123186 1102 62-63 1.3954985073576722 1102 64-65 1.7378858362020608 1102 66-67 1.297080095796332 1102 68-69 1.2793199544151648 1102 70-71 1.3883806263168879 1102 72-73 1.086913953977323 1102 74-75 1.2929122108650652 1102 76-77 1.3762289974319692 1102 78-79 1.509937700526141 1102 80-81 1.4217816237992231 1102 82-83 1.4292117028066151 1102 84-85 1.576814822719907 1102 86-87 1.5339811932318206 1102 88-89 1.451863511179159 1102 90-91 1.4128252363403782 1102 92-93 0.621371892496672 1102 94-95 1.1150809306664549 1102 96-97 1.0699506308312294 1102 98-99 1.2809521474079766 1102 100-101 0.1583568033042475 1103 1 0.06904235458953423 1103 2 0.04761663036681796 1103 3 0.3814312996018927 1103 4 -0.27252119916582274 1103 5 0.335027155222015 1103 6 0.513707574074104 1103 7 0.11853121418120338 1103 8 -0.3226276825337635 1103 9 0.1598070023994893 1103 10-11 -0.07500195179205349 1103 12-13 0.12313862782718132 1103 14-15 0.1756917015018118 1103 16-17 0.0033974401626508666 1103 18-19 0.3269116383307349 1103 20-21 -0.08149677533707944 1103 22-23 0.27171443486243874 1103 24-25 -0.3190372546868758 1103 26-27 -0.15870436430778057 1103 28-29 0.37519493428981576 1103 30-31 -0.13843647602266174 1103 32-33 -0.14948887357989804 1103 34-35 -0.12121745035930331 1103 36-37 0.3386628585952991 1103 38-39 0.5872229061740128 1103 40-41 0.29604731876697343 1103 42-43 -0.0483554360922156 1103 44-45 0.42977710058883645 1103 46-47 0.3625234682420171 1103 48-49 -0.7496258089386387 1103 50-51 -0.7259774371792744 1103 52-53 -0.15141613270972698 1103 54-55 0.13297769798673897 1103 56-57 -0.40295334872492106 1103 58-59 0.05518767145224501 1103 60-61 -0.325518409415281 1103 62-63 -0.08400076695291858 1103 64-65 0.37123126435881204 1103 66-67 -0.08299973003820327 1103 68-69 0.17209934483606304 1103 70-71 -0.33622024451040033 1103 72-73 -1.1333327804203535 1103 74-75 -0.9801284277416116 1103 76-77 -1.1934371854417627 1103 78-79 -0.49913341703553726 1103 80-81 -0.12538818752153347 1103 82-83 0.24651939511430854 1103 84-85 -0.4708992556545475 1103 86-87 -0.08339906801636587 1103 88-89 0.35861242264504867 1103 90-91 0.09932741340859508 1103 92-93 1.174165796705669 1103 94-95 1.3429474038159483 1103 96-97 -0.37834399906717664 1103 98-99 -1.081892555059369 1103 100-101 0.09449613566999204 1104 1 -1.064484495918446 1104 2 -0.3348216860337665 1104 3 -0.1098604275388908 1104 4 -0.8109827376273628 1104 5 -0.3826796662583902 1104 6 -0.5258424984948391 1104 7 -0.4268243736272126 1104 8 -1.3389556941447935 1104 9 0.10937158875653097 1104 10-11 -0.5425273509212261 1104 12-13 -0.2545827074413154 1104 14-15 -0.30870597629208874 1104 16-17 -0.06209602863270902 1104 18-19 -0.5297647041946618 1104 20-21 -0.15769416285521487 1104 22-23 -0.7233871616542515 1104 24-25 -0.17353652899746663 1104 26-27 -0.01284079391590609 1104 28-29 -0.5687455591789785 1104 30-31 -0.45447421187171244 1104 32-33 -0.6826891638556631 1104 34-35 -0.484787842231583 1104 36-37 -0.5767943257298143 1104 38-39 -0.5305201997766389 1104 40-41 -1.5916522458193825 1104 42-43 -1.6113089919703114 1104 44-45 -1.4811445249554325 1104 46-47 -1.1447334258073312 1104 48-49 -2.0560481601723097 1104 50-51 -2.2357742441458903 1104 52-53 -1.6029763649303348 1104 54-55 -1.9060281075285062 1104 56-57 -1.5020099524985042 1104 58-59 -2.5959371471254045 1104 60-61 -2.5274414863383576 1104 62-63 -2.2871938003346273 1104 64-65 -2.3294218561056326 1104 66-67 -2.539458365742128 1104 68-69 -2.441833891738682 1104 70-71 -2.0539270659908055 1104 72-73 -1.7784706614072903 1104 74-75 -2.0628570199041683 1104 76-77 -1.5372325410295709 1104 78-79 -1.9612161456276986 1104 80-81 -2.23206453004693 1104 82-83 -2.3400190664241514 1104 84-85 -1.4808774849723996 1104 86-87 -1.321788037537413 1104 88-89 -1.8173672580516147 1104 90-91 -1.827559379044235 1104 92-93 -2.042089645964868 1104 94-95 -1.5579959924104685 1104 96-97 -1.766587830707234 1104 98-99 -1.9786632372074067 1104 100-101 -1.8897201197726794 1105 1 -0.026022957456909523 1105 2 0.06902446781239036 1105 3 -0.0329373506158106 1105 4 -0.17636735301197604 1105 5 0.32885879528006967 1105 6 0.4549267322743944 1105 7 0.5154833186804808 1105 8 0.43027507508597296 1105 9 0.37860235798729747 1105 10-11 0.5151649567710805 1105 12-13 0.1588788310202247 1105 14-15 0.5182171006309844 1105 16-17 0.6494424329057509 1105 18-19 0.8452352958053382 1105 20-21 0.7076904525293983 1105 22-23 0.9496897614226683 1105 24-25 0.9803096248486796 1105 26-27 0.9871592060840939 1105 28-29 1.037023671590255 1105 30-31 0.9974488650513678 1105 32-33 0.7980800669135704 1105 34-35 1.0440583116145703 1105 36-37 1.0289749050394263 1105 38-39 1.2002490309925875 1105 40-41 1.3795016003344571 1105 42-43 1.4367679311066155 1105 44-45 1.0477016288907208 1105 46-47 0.09565118957728203 1105 48-49 1.1554903013661502 1105 50-51 1.1584565250848797 1105 52-53 1.618177481223512 1105 54-55 1.7862795847791872 1105 56-57 2.0460669705784156 1105 58-59 1.807909006720756 1105 60-61 1.5014046675077992 1105 62-63 0.9531908150499859 1105 64-65 1.3725012208174476 1105 66-67 1.4797724034886457 1105 68-69 0.9620122621074714 1105 70-71 0.47491908785534775 1105 72-73 0.7407601078234833 1105 74-75 0.9756045185573718 1105 76-77 0.5012289974319692 1105 78-79 0.5772453928338379 1105 80-81 0.9025508545684531 1105 82-83 1.2176732412681552 1105 84-85 1.3845071304122136 1105 86-87 0.7839811932318206 1105 88-89 0.6441712034868488 1105 90-91 0.9032098517249949 1105 92-93 1.2463718924966685 1105 94-95 1.2208501614356848 1105 96-97 1.0314890923696893 1105 98-99 0.5501829166387466 1105 100-101 0.3314337263811673 1106 1 0.4588671524332 1106 2 0.023694797482718855 1106 3 0.6250296823512187 1106 4 -0.022521199165822736 1106 5 0.13792472934600397 1106 6 0.6953113476590076 1106 7 0.4770217802189407 1106 8 0.36571463552553496 1106 9 -0.12826577388083393 1106 10-11 -0.15653833993221156 1106 12-13 0.014648061789451106 1106 14-15 -0.16241476750088424 1106 16-17 0.12334353180685298 1106 18-19 0.15979573536577618 1106 20-21 -0.13708976725082778 1106 22-23 -0.31335968912677714 1106 24-25 -0.38507499053593364 1106 26-27 -0.020395738970854893 1106 28-29 -0.030970833904255812 1106 30-31 0.018740073842572258 1106 32-33 -0.34271663638313754 1106 34-35 -0.5994856444293859 1106 36-37 -0.12006355649904066 1106 38-39 -0.2187070129634563 1106 40-41 0.5457103915432526 1106 42-43 0.484158040996725 1106 44-45 0.40484448603358203 1106 46-47 0.46309624452234743 1106 48-49 -0.27102068764483533 1106 50-51 0.22988509651344913 1106 52-53 0.9011445141905412 1106 54-55 0.8398510133506178 1106 56-57 0.6140614760729264 1106 58-59 0.7976068089185517 1106 60-61 0.9066244477275802 1106 62-63 1.3570369688961392 1106 64-65 1.403407814224046 1106 66-67 2.1260636122798573 1106 68-69 2.426984789579997 1106 70-71 1.930963043899304 1106 72-73 2.1906227451861113 1106 74-75 2.187829793282649 1106 76-77 2.023207019409991 1106 78-79 2.1363113268997758 1106 80-81 2.0289244809420843 1106 82-83 1.8090193951143085 1106 84-85 1.4854686688737537 1106 86-87 1.0538987756494045 1106 88-89 0.6256272474428926 1106 90-91 1.0295834780986226 1106 92-93 0.9283773870021612 1106 94-95 0.9687897218752468 1106 96-97 1.444263817644412 1106 98-99 0.630540059495889 1106 100-101 1.1219557044031454 1107 1 0.3457719143379663 1107 2 0.3312344800224025 1107 3 0.3095534918750289 1107 4 0.9655740389294181 1107 5 0.41768663410790907 1107 6 0.5068192841669443 1107 7 0.30241860561576317 1107 8 0.891508286319187 1107 9 0.8618135911985334 1107 10-11 0.49822356482968644 1107 12-13 0.5582988554402419 1107 14-15 0.2532598356737168 1107 16-17 0.49635940482272645 1107 18-19 0.6836052591753017 1107 20-21 0.420449915288863 1107 22-23 0.20747364420655146 1107 24-25 0.3212742158132684 1107 26-27 -0.1483719294470376 1107 28-29 -0.14307400850742624 1107 30-31 0.7955257881282876 1107 32-33 0.4023230461565461 1107 34-35 0.20507784763410797 1107 36-37 -0.041690540626028394 1107 38-39 0.059070764814322274 1107 40-41 0.035789756622612856 1107 42-43 -0.052548308209622974 1107 44-45 -0.0911872599981649 1107 46-47 0.4541676730937638 1107 48-49 0.5256062964821453 1107 50-51 0.36282160444995526 1107 52-53 0.5440016570476871 1107 54-55 0.16921609271569338 1107 56-57 0.4146567141681672 1107 58-59 0.37399569780744457 1107 60-61 0.5713069874101109 1107 62-63 1.2598147466739178 1107 64-65 0.7178919412081726 1107 66-67 1.1052302789465145 1107 68-69 0.5807546308498459 1107 70-71 0.905169393105659 1107 72-73 1.3344719515353276 1107 74-75 1.1630282059810604 1107 76-77 0.9775720987750738 1107 78-79 1.449803390391839 1107 80-81 1.2471784491960491 1107 82-83 1.198900347495261 1107 84-85 1.0876512085562915 1107 86-87 1.2017162359668632 1107 88-89 1.1682859776016237 1107 90-91 1.297440620955765 1107 92-93 1.903575799700576 1107 94-95 2.414226229811753 1107 96-97 2.381763817644412 1107 98-99 2.4311352975911262 1107 100-101 2.1705668155142597 1108 1 0.9791052476712991 1108 2 1.3010757498636707 1108 3 1.261934444255985 1108 4 1.4108121341675073 1108 5 0.8891152055364842 1108 6 1.0703113476590076 1108 7 1.1103551135522736 1108 8 1.126428921239821 1108 9 1.2324485118334536 1108 10-11 0.9196521362582644 1108 12-13 1.2479813951227854 1108 14-15 1.3893709467848367 1108 16-17 1.642391150854472 1108 18-19 1.3574147829848258 1108 20-21 1.0391007089396496 1108 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-0.9084699992235521 2316 78-79 0.083516295843868 2316 80-81 0.5956946672774848 2316 82-83 -0.08500234401612872 2316 84-85 -0.06616176590885559 2316 86-87 0.41065343403449717 2316 88-89 0.08020799278450852 2316 90-91 0.28657105573837427 2316 92-93 0.08249229383445922 2316 94-95 0.4691779206330082 2316 96-97 0.5005526375201903 2316 98-99 0.305617699247442 2316 100-101 0.5617848969497317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 157.0 8 403.0 9 276.0 10 259.0 11 263.0 12 312.0 13 287.0 14 257.0 15 239.0 16 346.0 17 575.0 18 629.0 19 829.0 20 1085.0 21 1209.0 22 1142.0 23 1003.0 24 861.0 25 706.0 26 631.0 27 658.0 28 729.0 29 702.0 30 782.0 31 881.0 32 1024.0 33 1253.0 34 1865.0 35 3937.0 36 5584.0 37 11254.0 38 19486.0 39 6062.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.687328746270474 22.209705900261827 14.520794008402849 18.58217134506485 2 16.39621262863058 24.342385678621444 39.51318273153504 19.74821896121293 3 17.10558363271022 31.28387018206174 32.96139560372648 18.64915058150155 4 10.938927114412714 21.632771113682033 39.71259818547159 27.71570358643366 5 10.497473056079889 40.27431041831578 35.42136028740182 13.806856238202519 6 23.35139743043293 41.731108810814106 18.720696584058942 16.196797174694026 7 21.47293429945808 38.63636363636363 24.124703160202156 15.765998903976131 8 24.14601473543202 34.78353528587956 22.13054862083663 18.93990135785179 9 26.86324057723924 15.957803081044878 22.564391402301652 34.61456493941424 10-11 22.38095962978749 28.830755647567436 28.654935151921084 20.133349570723986 12-13 24.343146806308226 29.745631127077875 28.42203007976618 17.489191986847715 14-15 22.204378006454363 29.730408573342267 28.220331242769287 19.844882177434087 16-17 17.98544723862875 32.8221092370456 29.53175424709249 19.66068927723315 18-19 17.518875966632162 30.594288497838395 33.77504110089509 18.111794434634355 20-21 19.105827193569993 29.783687511416918 33.96912866102417 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17.95271874809718 31.006058576386774 32.29236436704622 18.748858308469828 48-49 17.737319612738233 31.183401327406685 32.043475613468914 19.035803446386165 50-51 17.66120684406016 30.83328259148755 31.899622480667354 19.605888083784937 52-53 17.989252877062654 31.300614991170917 31.836448882664552 18.87368324910187 54-55 17.752542166473848 31.258752968397978 31.503074955854593 19.48562990927358 56-57 17.758631187968092 30.69095171405955 31.64312245022225 19.907294647750106 58-59 17.36284479084211 30.769347865797965 31.828076478109967 20.039730865249954 60-61 17.90933446995068 30.799792973269195 31.515252998843085 19.77561955793704 62-63 17.78527065700542 30.577543688729218 31.884399926931746 19.75278572733362 64-65 17.90933446995068 29.94352432564087 31.714668452779637 20.432472751628815 66-67 17.79744869999391 30.126194970468244 31.691834622176216 20.38452170736163 68-69 18.12853924374353 30.02572611581319 31.484046763685075 20.361687876758204 70-71 17.7464531449796 30.100316629117703 31.435334591731106 20.717895634171587 72-73 18.509864214820677 29.741825488643975 31.54950374474822 20.198806551787126 74-75 18.140717286732023 30.127717225841806 31.267125372952563 20.464440114473604 76-77 18.29674846252207 30.10792790598551 31.069993302076355 20.525330329416065 78-79 18.494641661085062 29.815654874261703 31.236680265481336 20.453023199171895 80-81 18.599677281860806 29.795104426718627 30.882755891128294 20.722462400292272 82-83 18.568471046702793 29.925257261158134 30.793703951774948 20.712567740364126 84-85 18.489313767277597 29.639834378615355 30.677251415697498 21.19360043840955 86-87 18.689490348900932 29.83011630031054 30.70236862936126 20.778024721427265 88-89 19.06092066004993 29.338427814650185 30.561560007306827 21.039091517993057 90-91 19.85097119892833 28.965475248127625 30.79674846252207 20.38680509042197 92-93 19.542714485782135 29.05985508128844 30.60951105157401 20.787919381355415 94-95 19.387444437678866 28.43953601656214 30.781525908786456 21.391493636972537 96-97 20.403549899531146 28.09931194057115 30.233513974304326 21.263624185593375 98-99 19.82889849601169 27.885435060585763 30.24873652803994 22.036929915362602 100-101 20.545880776959144 28.492814954636792 30.155878950252696 20.805425318151375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 728.0 1 440.0 2 168.5 3 196.0 4 145.0 5 103.5 6 173.5 7 348.5 8 516.0 9 540.5 10 395.0 11 244.5 12 215.0 13 210.0 14 207.5 15 197.5 16 187.0 17 193.0 18 215.0 19 236.5 20 261.0 21 288.0 22 320.0 23 363.0 24 412.0 25 485.5 26 602.0 27 693.5 28 785.0 29 969.0 30 1197.0 31 1443.5 32 1780.0 33 2072.0 34 2184.5 35 2280.5 36 2499.5 37 2616.5 38 2709.5 39 2987.0 40 3006.5 41 2824.0 42 2679.5 43 2446.5 44 2169.5 45 1972.5 46 1797.0 47 1567.5 48 1550.0 49 1627.5 50 1538.0 51 1367.0 52 1158.5 53 998.0 54 883.5 55 771.0 56 692.0 57 639.5 58 575.5 59 477.0 60 403.5 61 389.5 62 377.0 63 328.5 64 271.5 65 213.5 66 149.5 67 110.5 68 89.5 69 61.0 70 41.5 71 36.0 72 34.5 73 35.0 74 37.0 75 28.0 76 18.0 77 18.0 78 15.0 79 10.0 80 8.0 81 5.0 82 4.0 83 4.5 84 4.0 85 2.5 86 3.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 65692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.13864701942398 #Duplication Level Percentage of deduplicated Percentage of total 1 74.85265225933202 38.27863362357669 2 11.403822111091266 11.663520672227973 3 4.816336250520926 7.389027583267368 4 2.6522593320235757 5.425318151373074 5 1.5359885693873907 3.927418863788589 6 1.0269691016252902 3.15106862327224 7 0.8037149490980533 2.877062656031176 8 0.5626004643686373 2.3016501248249406 9 0.3720902542120617 1.7125372952566522 >10 1.9110555456331488 15.814711075930099 >50 0.029767220336964932 0.9270535224989345 >100 0.023813776269571946 2.7796383121232418 >500 0.00893016610108948 3.7523594958290203 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 860 1.309139621262863 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 853 1.2984838336479327 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 752 1.1447360409182243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 494 0.7519941545393655 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 361 0.5495341898556901 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 260 0.3957863971259819 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 173 0.26335017962613405 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 160 0.24356085976983496 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.23290507215490472 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 122 0.18571515557449916 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.15679230347683126 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.13700298362053218 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.10351336540217987 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0015222553735614686 0.0 16-17 0.0 0.0 0.0 0.0015222553735614686 0.0 18-19 0.0 0.0 0.0 0.004566766120684406 0.0 20-21 0.0 0.0 0.0 0.006089021494245875 0.0 22-23 0.0 0.0 0.0 0.012939170675272484 0.0 24-25 0.0 0.0 0.0 0.025878341350544967 0.0 26-27 0.0 0.0 0.0 0.05251781038787067 0.0 28-29 0.0 0.0 0.0 0.1217804298849175 0.0 30-31 0.0 0.0 0.0 0.22986056140778177 0.0 32-33 0.0 0.0 0.0 0.35240211897948004 0.0 34-35 0.0 0.0 0.0 0.5312671253729526 0.0 36-37 0.0 0.0 0.0 0.7733057297692261 0.0 38-39 0.0 0.0 0.0 1.068623272240151 0.0 40-41 0.0 0.0 0.0 1.443859221823053 0.0 42-43 0.0 0.0 0.0 1.6912257200267917 0.0 44-45 0.0 0.0 0.0 1.8898800462765633 0.0 46-47 0.0 0.0 0.0 2.0558058819947633 0.0 48-49 0.0 0.0 0.0 2.2422821652560434 0.0 50-51 0.0 0.0 0.0 2.4592035559885526 0.0 52-53 0.0 0.0 0.0 2.621323753272849 0.0 54-55 0.0 0.0 0.0 2.8489009316202885 0.0 56-57 0.0 0.0 0.0 3.122145771174572 0.0 58-59 0.0 0.0 0.0 3.36875114169153 0.0 60-61 0.0 0.0 0.0 3.589478170857943 0.0 62-63 0.0 0.0 0.0 3.802593923156549 0.0 64-65 0.0 0.0 0.0 3.975369908055775 0.0 66-67 0.0 0.0 0.0 4.2387200876819096 0.0 68-69 0.0 0.0 0.0 4.546215673141326 0.0 70-71 0.0 0.0 0.0 4.919168239663886 0.0 72-73 0.0 0.0 0.0 5.298970955367473 0.0 74-75 0.0 0.0 0.0 5.655939840467637 0.0 76-77 0.0 0.0 0.0 5.984747001156914 0.0 78-79 0.0 0.0 0.0 6.341715886257079 0.0 80-81 0.0 0.0 0.0 6.748158070997991 0.0 82-83 0.0 0.0 0.0 7.177434086342325 0.0 84-85 0.0 0.0 0.0 7.6112768678073435 0.0 86-87 0.0 0.0 0.0 8.124276928697558 0.0 88-89 0.0 0.0 0.0 8.620532180478598 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTATAC 25 0.0050002667 57.000004 3 ATACTTA 35 2.7685764E-4 54.28571 6 GGTATCA 510 0.0 49.362743 1 CCCCGCG 20 5.370653E-4 47.500004 90-91 CGCAGAA 50 2.7977392E-5 47.500004 9 GGATGAC 50 0.0016047396 38.000004 8 AATACCT 50 0.0016047396 38.000004 5 GTATCAA 795 0.0 34.056602 1 ATTAGCC 35 2.0434771E-4 33.92857 26-27 CGAAACC 30 0.00393833 31.666668 74-75 GACACAA 30 0.00393833 31.666668 48-49 ACCCTAC 65 0.0058045457 29.23077 1 CTACACG 65 0.0058045457 29.23077 4 CGGAGGT 65 0.0058045457 29.23077 9 TACACGG 65 0.0058045457 29.23077 5 AACGCAG 940 0.0 27.792553 6 TATCAAC 975 0.0 27.282051 2 CAACGCA 945 0.0 27.142857 5 TCAACGC 945 0.0 27.142857 4 ACACGGA 70 0.00833507 27.142855 6 >>END_MODULE