FastQCFastQC Report
Fri 10 Feb 2017
SRR2031806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65692
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT14562.2164038239054986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10411.5846678438774888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10141.5435669487913293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.896608415027705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.876819095171406No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.8052730926140169No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT3480.5297448699993911TruSeq Adapter, Index 11 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.5282226146258296No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.4170979723558424No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG2480.3775193326432442TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT2230.33946294830420753RNA PCR Primer, Index 11 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.1902819216951836No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.1796261340802533No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.17353711258600743No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.15679230347683126No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT920.14004749436765512TruSeq Adapter, Index 11 (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.1309139621262863No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA730.11112464226998721RNA PCR Primer, Index 11 (96% over 28bp)
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.10046885465505692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA603.2196112E-1063.3333369
GGTATCA6050.058.099181
TACCCAT250.005000266757.0000042
CATAACT250.005000266757.0000044
GCAGATC250.005000266757.0000048
ATGGGGA804.120011E-947.5000045
TATAACA405.350914E-447.5000044
GTATCAA8400.041.8452382
TATCAAC9800.035.8673483
AACGCAG9700.035.2577327
CAACGCA9900.035.0252536
ATCAACG10100.034.801984
TCAACGC10100.034.801985
ACGCAGA10100.034.3316848
TGGGGAA702.0323922E-433.928576
GCAGAAA501.287608E-633.25000410-11
CGCAGAG9700.031.8298999
CATGGGG1401.6547347E-830.5357114
CAGAAAA701.6816557E-830.53571110-11
CAAGTTG350.00836500127.14285530-31
Produced by FastQC (version 0.11.5)