##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031806_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65692 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75476465931925 34.0 31.0 34.0 31.0 34.0 2 32.87135419838032 34.0 33.0 34.0 31.0 34.0 3 32.86479327772027 34.0 33.0 34.0 31.0 34.0 4 36.290050538878404 37.0 37.0 37.0 35.0 37.0 5 36.32997929732692 37.0 37.0 37.0 35.0 37.0 6 36.43773975522134 37.0 37.0 37.0 35.0 37.0 7 36.37086707666078 37.0 37.0 37.0 35.0 37.0 8 36.434162455093464 37.0 37.0 37.0 35.0 37.0 9 38.20632649333252 39.0 39.0 39.0 37.0 39.0 10-11 38.226800828106924 39.0 39.0 39.0 37.0 39.0 12-13 38.236862936126165 39.0 39.0 39.0 37.0 39.0 14-15 39.68769408756013 41.0 40.0 41.0 37.0 41.0 16-17 39.60974700115692 41.0 39.5 41.0 37.0 41.0 18-19 39.696751507032815 41.0 40.0 41.0 37.0 41.0 20-21 39.69640899957377 41.0 40.0 41.0 37.0 41.0 22-23 39.604365828411375 41.0 40.0 41.0 35.5 41.0 24-25 39.495844242830174 41.0 39.5 41.0 35.0 41.0 26-27 39.29489892224319 41.0 39.0 41.0 35.0 41.0 28-29 39.17083510929794 41.0 39.0 41.0 35.0 41.0 30-31 38.84571941788955 40.5 38.0 41.0 35.0 41.0 32-33 38.221366376423305 40.0 36.5 41.0 35.0 41.0 34-35 38.19270991901601 40.0 36.5 41.0 35.0 41.0 36-37 38.02625129391707 40.0 36.0 41.0 35.0 41.0 38-39 37.754277537599705 40.0 35.5 41.0 34.0 41.0 40-41 37.606124033367834 40.0 35.0 41.0 34.0 41.0 42-43 37.34319247396944 40.0 35.0 41.0 33.0 41.0 44-45 36.97415971503379 40.0 35.0 41.0 32.5 41.0 46-47 36.54360500517567 40.0 35.0 41.0 30.5 41.0 48-49 36.152088534372524 40.0 35.0 41.0 26.5 41.0 50-51 35.87641569749741 40.0 35.0 41.0 23.5 41.0 52-53 35.8694437678865 40.0 35.0 41.0 23.0 41.0 54-55 35.678530110211284 40.0 35.0 41.0 21.5 41.0 56-57 35.452985142787554 40.0 35.0 41.0 20.0 41.0 58-59 35.22242434390793 39.5 35.0 41.0 18.0 41.0 60-61 34.885579674846255 39.0 35.0 41.0 16.0 41.0 62-63 34.516006515253 39.0 35.0 41.0 13.5 41.0 64-65 34.15818516714364 38.0 35.0 41.0 10.5 41.0 66-67 33.720239907446874 37.0 35.0 40.5 10.0 41.0 68-69 33.101496377032205 37.0 34.0 40.0 10.0 41.0 70-71 32.541116117639895 36.0 34.0 39.0 9.0 41.0 72-73 32.03313949948243 36.0 33.5 39.0 8.0 41.0 74-75 31.62686476283261 35.0 33.0 38.0 8.0 40.5 76-77 30.34382420994946 34.5 30.5 36.5 7.5 39.0 78-79 30.922463922547646 35.0 32.0 37.0 7.0 39.0 80-81 30.7551300006089 35.0 33.0 36.0 7.0 39.0 82-83 30.475263350179624 35.0 32.0 36.0 7.0 37.0 84-85 30.241901601412653 35.0 32.0 36.0 7.0 37.0 86-87 30.024531145344945 35.0 32.0 35.0 7.0 37.0 88-89 29.853186080496865 35.0 31.0 35.0 7.0 36.0 90-91 29.645542836266213 35.0 31.0 35.0 7.0 36.0 92-93 29.484198989222435 35.0 31.0 35.0 7.0 36.0 94-95 29.36330146745418 35.0 31.0 35.0 7.0 36.0 96-97 29.281472629848384 35.0 30.0 35.0 7.0 35.0 98-99 29.181391950313586 35.0 30.0 35.0 7.0 35.0 100-101 28.2537599707727 34.0 27.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5496155070848872 1101 2 0.5583395164605705 1101 3 0.6124790357605647 1101 4 0.0015123665716458845 1101 5 0.3827672562827047 1101 6 0.3159505446682971 1101 7 0.26186325368757224 1101 8 0.22566686531650504 1101 9 0.4638208533291035 1101 10-11 0.37593746470995626 1101 12-13 0.27057267478795666 1101 14-15 0.40188836456961496 1101 16-17 0.5470563809400986 1101 18-19 0.43964712508492454 1101 20-21 0.5207040853283331 1101 22-23 0.42024940189593707 1101 24-25 0.4481530888868406 1101 26-27 0.5402498375973934 1101 28-29 0.8179787339714721 1101 30-31 0.6719599477833356 1101 32-33 1.2847100499314053 1101 34-35 1.2282655535485176 1101 36-37 0.9744224057398156 1101 38-39 1.4179795758413363 1101 40-41 1.4112344142825037 1101 42-43 1.1295491788584613 1101 44-45 1.7197487599265955 1101 46-47 2.105538387812942 1101 48-49 1.0845800324925534 1101 50-51 1.3248954574842244 1101 52-53 1.9139711910864747 1101 54-55 1.7106041551582507 1101 56-57 2.0958233095649916 1101 58-59 2.070397981364806 1101 60-61 2.0051193325749566 1101 62-63 2.525040391896667 1101 64-65 2.2666063539013805 1101 66-67 1.8007510163433338 1101 68-69 1.619953371724911 1101 70-71 1.8315189360905038 1101 72-73 2.4649306577311734 1101 74-75 1.4975346023908358 1101 76-77 1.4069189754437232 1101 78-79 1.2727323084890365 1101 80-81 0.6221830443660359 1101 82-83 1.5890638103160768 1101 84-85 1.1224112533831594 1101 86-87 0.8676282968691034 1101 88-89 1.6401623819977225 1101 90-91 2.4836212807284177 1101 92-93 1.0477283815741387 1101 94-95 1.7470842453793516 1101 96-97 1.3110829282417384 1101 98-99 1.7578776080774325 1101 100-101 1.5505775241875845 1102 1 0.19705140452078496 1102 2 -0.13076304764199875 1102 3 0.021132881914411428 1102 4 -0.017718402659120613 1102 5 -0.1733224873070398 1102 6 -0.07026740404965182 1102 7 -0.09871366938934756 1102 8 -0.07721775006810816 1102 9 -0.012140685132436602 1102 10-11 -0.02790868913619704 1102 12-13 0.10550857222386156 1102 14-15 0.21198451841576826 1102 16-17 0.24016535529907657 1102 18-19 -0.07157082363301726 1102 20-21 0.07999895712319471 1102 22-23 0.10774940189593707 1102 24-25 -0.08950716752341492 1102 26-27 -0.2506155470179934 1102 28-29 0.24345950320223864 1102 30-31 0.25929969137308717 1102 32-33 0.48983825505961676 1102 34-35 0.43499632277929123 1102 36-37 -0.06484041477300906 1102 38-39 1.1038770117387813 1102 40-41 0.9448882604363504 1102 42-43 0.5934914865507679 1102 44-45 1.3030820932599312 1102 46-47 0.9316601826847375 1102 48-49 0.8666313145438309 1102 50-51 0.9915621241508958 1102 52-53 0.8041955500608324 1102 54-55 1.0086810782351705 1102 56-57 1.3225861300778163 1102 58-59 0.7707184941853171 1102 60-61 1.1533565120621319 1102 62-63 1.8191109047171778 1102 64-65 2.102343533388556 1102 66-67 1.8800779394202536 1102 68-69 1.844312346083889 1102 70-71 1.3251086796802554 1102 72-73 1.17887296542348 1102 74-75 1.8789448588010913 1102 76-77 1.6913741036488474 1102 78-79 0.8833092315659599 1102 80-81 1.2079202238532183 1102 82-83 1.5193522718545367 1102 84-85 1.924494586716495 1102 86-87 2.6520834250742276 1102 88-89 1.66019443327977 1102 90-91 1.1302558961130345 1102 92-93 1.675933509779263 1102 94-95 1.0700009120460159 1102 96-97 1.3150893384981508 1102 98-99 1.4101211978210202 1102 100-101 0.6547441908542488 1103 1 0.31062179639305754 1103 2 0.23208165482535037 1103 3 0.533469601798302 1103 4 0.4118897250622098 1103 5 0.27624209905000185 1103 6 0.26209834341043603 1103 7 -0.029410331218088004 1103 8 -0.12221049317405885 1103 9 -0.18594329761429407 1103 10-11 0.13772991754014896 1103 12-13 0.051626133907461735 1103 14-15 -0.050352201468115254 1103 16-17 -0.0297911033366276 1103 18-19 0.1428703955251791 1103 20-21 0.2726694941333605 1103 22-23 0.5558626094431034 1103 24-25 0.21328673668558196 1103 26-27 0.6434337998615476 1103 28-29 0.14875703585826727 1103 30-31 0.3969992559594431 1103 32-33 0.40892388640939714 1103 34-35 -0.032347653998648696 1103 36-37 0.3295717768089901 1103 38-39 0.3299292613759306 1103 40-41 0.6105268671126964 1103 42-43 0.08296899017921788 1103 44-45 0.15449718759956 1103 46-47 0.2790839852972198 1103 48-49 0.034265567083743065 1103 50-51 -0.39523032867929686 1103 52-53 -0.7395272365864898 1103 54-55 -0.525244901445518 1103 56-57 -0.2322034199947538 1103 58-59 -0.08015233310060665 1103 60-61 0.2812592696818683 1103 62-63 0.32908913403502993 1103 64-65 0.36939723440452354 1103 66-67 -0.1609235119585506 1103 68-69 0.26775211386328124 1103 70-71 -0.2966257179975429 1103 72-73 0.09759575207079507 1103 74-75 -0.501679234087149 1103 76-77 -0.45412662204055465 1103 78-79 -0.5208997669826623 1103 80-81 -0.06747890531949707 1103 82-83 0.37621003673117315 1103 84-85 0.0141172282259312 1103 86-87 -0.3920022062755528 1103 88-89 0.5067111241360855 1103 90-91 -0.21626079474328108 1103 92-93 0.12477240673136691 1103 94-95 0.7020763837441315 1103 96-97 1.1131662615750741 1103 98-99 0.3974216332346643 1103 100-101 -0.043369016692921036 1104 1 0.27397448144385805 1104 2 0.15769849081954135 1104 3 -0.17117481039328197 1104 4 0.09766621272549258 1104 5 -0.019476333460879403 1104 6 -0.18565201943427212 1104 7 0.07436325368757224 1104 8 -0.07721775006810816 1104 9 0.1032439302521837 1104 10-11 0.029783618556116664 1104 12-13 0.06704703376232146 1104 14-15 0.32736913380038857 1104 16-17 0.32670381683753646 1104 18-19 0.20727533021312894 1104 20-21 -0.05461642749218498 1104 22-23 -0.007635213488676129 1104 24-25 0.03549283247658508 1104 26-27 0.2589998375973934 1104 28-29 0.32038258012531884 1104 30-31 0.11506892214231357 1104 32-33 0.08599210121346346 1104 34-35 0.41576555354851763 1104 36-37 0.34862112368853104 1104 38-39 0.09426162712339448 1104 40-41 0.08911902966711693 1104 42-43 -0.1661238980646189 1104 44-45 -0.11037944520160892 1104 46-47 -0.5394936634691092 1104 48-49 0.4147082376207507 1104 50-51 0.28002366261243594 1104 52-53 0.13111862698391263 1104 54-55 0.1817580013120903 1104 56-57 0.30335536084704273 1104 58-59 -1.0273584288916098 1104 60-61 -1.3754896417840214 1104 62-63 -0.9212737106674354 1104 64-65 -0.6668872358422107 1104 66-67 -0.2641528298105129 1104 68-69 -0.41530303853149775 1104 70-71 -0.41527593570436494 1104 72-73 -0.6865116499611332 1104 74-75 -0.774901295045062 1104 76-77 -1.5105489732742257 1104 78-79 -1.5782292299725036 1104 80-81 -0.9651566992237051 1104 82-83 -0.019109266606999853 1104 84-85 -0.9697361825142714 1104 86-87 -0.2902242672334623 1104 88-89 -0.5513440282586899 1104 90-91 0.3514097422668776 1104 92-93 -0.6221434132976569 1104 94-95 -0.7184606264155242 1104 96-97 -0.4926029691941558 1104 98-99 -0.7533403406405199 1104 100-101 -0.5087173476072913 1105 1 0.25474371221309156 1105 2 0.11923695235800125 1105 3 0.07882518960671803 1105 4 0.1361277511870327 1105 5 0.1151390511545003 1105 6 -0.12795971174195842 1105 7 0.01667094599526564 1105 8 0.05739763454727154 1105 9 -0.3198329928247432 1105 10-11 -0.37406253529004374 1105 12-13 -0.26949142777613844 1105 14-15 -0.25916932773807844 1105 16-17 -0.07714233700861683 1105 18-19 -0.28310928517148426 1105 20-21 -0.12192411979987838 1105 22-23 0.2519801711267107 1105 24-25 0.05472360170734447 1105 26-27 -0.2794617008641396 1105 28-29 -0.27577126602852786 1105 30-31 0.02853046060385367 1105 32-33 0.45137671659807665 1105 34-35 -0.32461906183609557 1105 36-37 0.002467277534677237 1105 38-39 -0.3191999113381456 1105 40-41 -0.46857327802518967 1105 42-43 -0.5603546672953854 1105 44-45 -0.10076406058622212 1105 46-47 -0.924109048084496 1105 48-49 -0.4122148393023224 1105 50-51 -0.33536095277218436 1105 52-53 -0.30157368070840107 1105 54-55 -0.3182419986879097 1105 56-57 -0.18702925453757047 1105 58-59 -0.21005073658391638 1105 60-61 -0.09664348793786814 1105 62-63 0.04988013548641135 1105 64-65 -0.8591949281499041 1105 66-67 -0.3699220605797464 1105 68-69 -0.5595338077622642 1105 70-71 -0.953737474165905 1105 72-73 -0.47497318842267333 1105 74-75 -1.1306705258142884 1105 76-77 -1.4913182040434592 1105 78-79 -0.9916907684340401 1105 80-81 -0.7440028530698619 1105 82-83 0.25973688723915345 1105 84-85 -0.1908900286681181 1105 86-87 -0.39599349800269223 1105 88-89 -0.859036335951 1105 90-91 -1.2351287192715823 1105 92-93 -0.7952203363745838 1105 94-95 -1.0069221648770608 1105 96-97 -0.3387568153480025 1105 98-99 -0.9071864944866732 1105 100-101 -1.326025039914981 1106 1 0.26985360232298206 1106 2 0.18929189741295005 1106 3 0.44283617861771063 1106 4 0.1443695094287918 1106 5 -0.060685124669674906 1106 6 0.22368863990639198 1106 7 0.11007753940185694 1106 8 -0.015404563254925563 1106 9 0.0441779961862494 1106 10-11 0.16165175042424096 1106 12-13 0.10688219859748926 1106 14-15 -0.11150449257324624 1106 16-17 0.030687333321054666 1106 18-19 -0.14368620824840406 1106 20-21 -0.22631972419548418 1106 22-23 0.16132083046736767 1106 24-25 0.06571261269636608 1106 26-27 0.16971412331167812 1106 28-29 0.03672873397147214 1106 30-31 -0.049766242692854235 1106 32-33 0.20137671659807665 1106 34-35 0.08540841069137883 1106 36-37 0.11579145335885954 1106 38-39 0.611431956793723 1106 40-41 0.44694869999678843 1106 42-43 0.11615632171560009 1106 44-45 0.7137963789742159 1106 46-47 1.0504788640034164 1106 48-49 0.6798181277306483 1106 50-51 1.348704981293757 1106 52-53 1.5315307148960002 1106 54-55 0.9338184408725354 1106 56-57 0.8562399762316559 1106 58-59 1.5495646480314704 1106 60-61 1.575059808765424 1106 62-63 1.990814201420477 1106 64-65 1.3901182586632785 1106 66-67 0.8677153020576185 1106 68-69 0.9205486098201519 1106 70-71 0.39104274561431396 1106 72-73 -0.34310505655454193 1106 74-75 0.6701536500098868 1106 76-77 -0.46659292931818186 1106 78-79 -0.2629819772252482 1106 80-81 0.495694949127941 1106 82-83 1.4417423817446462 1106 84-85 0.6298517295736374 1106 86-87 0.7619735349643371 1106 88-89 0.8737933343786715 1106 90-91 1.398799852156987 1106 92-93 0.9376093339550877 1106 94-95 1.2664294834745853 1106 96-97 0.8185234044322165 1106 98-99 0.6745442747440968 1106 100-101 -0.0863272377171782 1107 1 0.3750123324817096 1107 2 0.3698474529685072 1107 3 0.31783617861771063 1107 4 0.33881395387323465 1107 5 0.09804503406048326 1107 6 0.2435299097476573 1107 7 -0.04468436536004816 1107 8 0.1254684526180938 1107 9 0.18901926602751473 1107 10-11 0.19538190915439912 1107 12-13 0.2338663255816158 1107 14-15 -0.1363060798748279 1107 16-17 -0.004034888901166767 1107 18-19 0.07159156952937451 1107 20-21 -0.04179591467166688 1107 22-23 -0.6501871060405691 1107 24-25 0.14904594602969468 1107 26-27 0.2689204725180261 1107 28-29 0.5178795276222701 1107 30-31 0.17642423349762737 1107 32-33 0.1855037007250644 1107 34-35 0.030844918627884965 1107 36-37 -0.15305775299034963 1107 38-39 0.5449637028254628 1107 40-41 0.6046867952348833 1107 42-43 0.7977039407632205 1107 44-45 1.1423678075456465 1107 46-47 1.265756641781202 1107 48-49 0.47346892138143915 1107 50-51 1.2614033939921683 1107 52-53 1.383713254578538 1107 54-55 1.5121914567455548 1107 56-57 1.1488987063903835 1107 58-59 1.595199568666395 1107 60-61 1.7575994913051076 1107 62-63 2.3162110268172995 1107 64-65 2.6133325443775632 1107 66-67 2.3816041909465113 1107 68-69 1.8024930542645947 1107 70-71 1.9208046503762262 1107 72-73 1.6410219275724387 1107 74-75 1.7534869833432225 1107 76-77 1.5353911976659447 1107 78-79 1.4681688164255426 1107 80-81 1.2149409808739797 1107 82-83 1.8058296833319503 1107 84-85 2.2935422057641155 1107 86-87 1.9028465508373564 1107 88-89 1.938277461362798 1107 90-91 1.653760169617307 1107 92-93 1.3691569530027081 1107 94-95 1.1493659914110985 1107 96-97 1.4464995949084098 1107 98-99 0.9354569731567928 1107 100-101 0.662680698790755 1108 1 0.2662821737515486 1108 2 0.41667284979390473 1108 3 0.6083123690938947 1108 4 0.31817903323831587 1108 5 0.22026725628271038 1108 6 0.19511721133495996 1108 7 0.0410299203542408 1108 8 -0.2618331346834921 1108 9 0.32632085332910066 1108 10-11 0.33427079804328486 1108 12-13 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1.4440573095339495 2316 90-91 0.036610411163199075 2316 92-93 0.49700374389297863 2316 94-95 0.3580443903068833 2316 96-97 0.8631662615750741 2316 98-99 0.4707399269180108 2316 100-101 0.27974419085424884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 14.0 15 36.0 16 125.0 17 341.0 18 511.0 19 711.0 20 942.0 21 1247.0 22 1352.0 23 1128.0 24 943.0 25 838.0 26 969.0 27 989.0 28 1175.0 29 1343.0 30 937.0 31 838.0 32 1040.0 33 1140.0 34 1597.0 35 3541.0 36 4899.0 37 11146.0 38 21300.0 39 6577.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97975486310141 21.586682114763097 15.064942984328313 20.368620037807183 2 15.769043414723255 23.518845521524693 41.34750045667661 19.364610607075445 3 16.23028679291238 30.70236862936126 35.50356207757414 17.563782500152225 4 9.687633197345187 23.21896121293308 39.22090969981124 27.872495889910493 5 9.669366132862448 39.42945868598916 37.72605492297388 13.17512025817451 6 22.366498203738658 42.58204956463496 18.504536321013212 16.546915910613162 7 19.606649211471716 37.977227059611515 24.483955428362663 17.932168300554103 8 23.502100712415512 35.33002496498813 23.186993850088292 17.98088047250807 9 24.890397613103573 14.697375631735978 22.830786092674906 37.58144066248554 10-11 21.34202033733179 28.456280825671314 30.11934482128722 20.082354015709676 12-13 23.51503988309079 28.285027096145647 29.52642635328503 18.673506667478538 14-15 21.915910613164463 28.127473664982038 28.622206661389516 21.334409060463983 16-17 18.2404250137003 32.315198197649636 29.850666747853623 19.593710040796445 18-19 17.849205382695 28.413657675211596 34.35882603665591 19.378310905437495 20-21 18.289898313341048 27.25902697436522 35.367320221640384 19.08375449065335 22-23 18.00447543079827 29.232631066187665 34.65490470681362 18.10798879620045 24-25 18.243469524447423 30.33854959508007 34.72036168787676 16.69761919259575 26-27 18.257930950496252 30.258631187968092 34.51485721244596 16.96858064908969 28-29 16.746331364549714 27.600012178042988 36.1923826341107 19.461273823296597 30-31 18.340893868355355 27.991231809048283 36.53489009316203 17.13298422943433 32-33 16.779820982768072 30.02800949887353 35.6824270839676 17.509742434390795 34-35 16.548438165986727 30.13000060890215 36.64068684162455 16.680874383486575 36-37 18.529653534676978 29.40312366802655 34.743956646166964 17.323266151129513 38-39 17.73655848505145 28.977653291116116 35.20900566278999 18.07678256104244 40-41 16.903884795713328 28.635145832064783 35.9906837971138 18.47028557510808 42-43 17.601077756804482 28.795743773975524 36.1725933142544 17.4305851549656 44-45 16.54234914449248 29.885678621445532 35.985355903306335 17.586616330755646 46-47 18.198562990927357 28.467697740973026 33.7111063752055 19.622632892894114 48-49 17.930782521330766 28.447363814192094 33.76464230532108 19.857211359156054 50-51 16.984693605717634 28.647541919426413 34.20686085718852 20.16090361766743 52-53 17.207574742738842 29.56143822687694 34.883243012847835 18.34774401753638 54-55 17.005114778055166 29.408451561834013 33.47591791999026 20.11051574012056 56-57 18.04557632588443 29.607105888083783 32.28018632405772 20.06713146197406 58-59 16.402301650124826 30.05084332947695 34.07416428179991 19.47269073859831 60-61 17.91999025756561 31.709340558972173 31.274736649820372 19.095932533641843 62-63 16.706752724837116 32.41718930767826 31.56244291542349 19.313615052061134 64-65 16.47993667417646 32.02673080435974 31.3447603970042 20.148572124459598 66-67 16.285849114047373 32.44535103208914 31.040309322291908 20.228490531571577 68-69 16.845406178881593 31.95390575645251 30.765015260726276 20.435672803939628 70-71 16.773731961273825 31.20927966875723 31.12860013395847 20.888388236010474 72-73 17.304237958959995 30.543292942824092 31.37139986604153 20.78106923217439 74-75 17.062960482250503 30.53339828289594 30.885800401875418 21.51784083297814 76-77 16.934329903184558 30.92081227546733 31.037264811544784 21.107593009803324 78-79 17.514309200511477 30.072916032393593 31.089782621932656 21.322992145162274 80-81 17.106344760397004 30.315715764476646 30.61864458381538 21.959294891310964 82-83 17.191591061316448 30.000608902149423 30.53339828289594 22.27440175363819 84-85 16.83233879315594 30.01430920051148 30.270809230956587 22.882542775375995 86-87 16.675546489679107 30.027248371186747 30.902545210984595 22.394659928149547 88-89 17.139834378615358 29.524904097911463 30.547859708944774 22.787401814528405 90-91 17.45646349631614 29.141295743773977 30.616361200755037 22.785879559154846 92-93 16.92595749862997 29.29199902575656 30.68942945868599 23.092614016927477 94-95 16.731869938500882 28.583389149363697 30.83708822992145 23.84765268221397 96-97 17.345338854046155 28.46541435791268 30.548620836631553 23.640625951409607 98-99 16.83157766546916 28.44943067649029 30.359861170309934 24.35913048773062 100-101 16.79961030262437 28.90382390549839 30.773914631918654 23.522651159958592 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 766.0 1 453.0 2 181.5 3 223.5 4 156.5 5 109.0 6 159.5 7 316.5 8 506.0 9 553.5 10 404.5 11 242.0 12 213.5 13 218.0 14 204.5 15 190.0 16 192.0 17 186.0 18 187.0 19 210.5 20 242.5 21 263.0 22 290.0 23 345.0 24 384.5 25 435.0 26 531.5 27 617.0 28 731.0 29 888.5 30 1115.0 31 1375.5 32 1698.0 33 2014.0 34 2224.5 35 2365.5 36 2577.5 37 2779.5 38 2906.5 39 3220.5 40 3287.5 41 3092.5 42 2916.5 43 2642.5 44 2339.5 45 2135.0 46 1963.0 47 1696.5 48 1546.5 49 1549.0 50 1563.5 51 1449.0 52 1149.5 53 910.0 54 769.0 55 656.0 56 573.0 57 501.0 58 464.5 59 380.0 60 303.0 61 279.5 62 262.5 63 240.0 64 185.0 65 135.5 66 92.5 67 63.0 68 54.0 69 35.0 70 20.0 71 16.5 72 20.5 73 20.5 74 16.5 75 16.0 76 11.5 77 8.0 78 8.0 79 7.5 80 6.5 81 5.0 82 2.5 83 1.5 84 1.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.146136515861901 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 7.611276867807343E-4 50-51 7.611276867807343E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.611276867807343E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 65692.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.33733179078122 #Duplication Level Percentage of deduplicated Percentage of total 1 71.17483127958903 32.268769408756015 2 12.987274619749522 11.776167569871522 3 5.513212235167713 7.498629970163795 4 2.9882819057851795 5.419229129878828 5 1.7426048416882116 3.9502526943920113 6 1.2053856226706512 3.278938074651404 7 0.9199879125675721 2.919685806490897 8 0.6681664036530908 2.4234305547098582 9 0.5305039787798408 2.164647141204408 >10 2.1790954571399794 16.320099859952506 >50 0.04029144142631703 1.1645253607745236 >100 0.030218581069737765 2.8922852097667904 >500 0.010072860356579257 2.578700602813128 >1k 0.010072860356579257 5.344638616574317 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1456 2.2164038239054986 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1041 1.5846678438774888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1014 1.5435669487913293 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 589 0.896608415027705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 576 0.876819095171406 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.8052730926140169 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 348 0.5297448699993911 TruSeq Adapter, Index 11 (95% over 21bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 347 0.5282226146258296 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.4170979723558424 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 248 0.3775193326432442 TruSeq Adapter, Index 11 (96% over 28bp) ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 223 0.33946294830420753 RNA PCR Primer, Index 11 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.1902819216951836 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 0.1796261340802533 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.17353711258600743 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.15679230347683126 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 92 0.14004749436765512 TruSeq Adapter, Index 11 (96% over 26bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.1309139621262863 No Hit CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA 73 0.11112464226998721 RNA PCR Primer, Index 11 (96% over 28bp) GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.10046885465505692 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0015222553735614686 0.0 16-17 0.0 0.0 0.0 0.0015222553735614686 0.0 18-19 0.0 0.0 0.0 0.003044510747122937 0.0 20-21 0.0 0.0 0.0 0.004566766120684406 0.0 22-23 0.0 0.0 0.0 0.011416915301711014 0.0 24-25 0.0 0.0 0.0 0.0243560859769835 0.0 26-27 0.0 0.0 0.0 0.04947329964074773 0.0 28-29 0.0 0.0 0.0 0.12101930219813675 0.0 30-31 0.0 0.0 0.0 0.23138281678134323 0.0 32-33 0.0 0.0 0.0 0.35316324666626076 0.0 34-35 0.0 0.0 0.0 0.5396395299275407 0.0 36-37 0.0 0.0 0.0 0.7824392620105949 0.0 38-39 0.0 0.0 0.0 1.0762345491079583 0.0 40-41 0.0 0.0 0.0 1.4491871156305183 0.0 42-43 0.0 0.0 0.0 1.7064482737624065 0.0 44-45 0.0 0.0 0.0 1.9096693661328623 0.0 46-47 0.0 0.0 0.0 2.0809230956585276 0.0 48-49 0.0 0.0 0.0 2.2704438896669306 0.0 50-51 0.0 0.0 0.0 2.4850818973390973 0.0 52-53 0.0 0.0 0.0 2.647202094623394 0.0 54-55 0.0 0.0 0.0 2.8785849114047375 0.0 56-57 0.0 0.0 0.0 3.157157644766486 0.0 58-59 0.0 0.0 0.0 3.404524142970225 0.0 60-61 0.0 0.0 0.0 3.629056810570541 0.0 62-63 0.0 0.0 0.0 3.83456128600134 0.0 64-65 0.0 0.0 0.0 4.005053887840225 0.0 66-67 0.0 0.0 0.0 4.271448578213481 0.0 68-69 0.0 0.0 0.0 4.576660780612555 0.0 70-71 0.0 0.0 0.0 4.9518967301954575 0.0 72-73 0.0 0.0 0.0 5.335505084332947 0.0 74-75 0.0 0.0 0.0 5.707696523168726 0.0 76-77 0.0 0.0 0.0 6.038787066918346 0.0 78-79 0.0 0.0 0.0 6.390428058211045 0.0 80-81 0.0 0.0 0.0 6.795347987578396 0.0 82-83 0.0 0.0 0.0 7.225385130609511 0.0 84-85 0.0 0.0 0.0 7.661511295134872 0.0 86-87 0.0 0.0 0.0 8.179078122145771 0.0 88-89 0.0 0.0 0.0 8.683705778481398 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 60 3.2196112E-10 63.333336 9 GGTATCA 605 0.0 58.09918 1 TACCCAT 25 0.0050002667 57.000004 2 CATAACT 25 0.0050002667 57.000004 4 GCAGATC 25 0.0050002667 57.000004 8 ATGGGGA 80 4.120011E-9 47.500004 5 TATAACA 40 5.350914E-4 47.500004 4 GTATCAA 840 0.0 41.845238 2 TATCAAC 980 0.0 35.867348 3 AACGCAG 970 0.0 35.257732 7 CAACGCA 990 0.0 35.025253 6 ATCAACG 1010 0.0 34.80198 4 TCAACGC 1010 0.0 34.80198 5 ACGCAGA 1010 0.0 34.331684 8 TGGGGAA 70 2.0323922E-4 33.92857 6 GCAGAAA 50 1.287608E-6 33.250004 10-11 CGCAGAG 970 0.0 31.829899 9 CATGGGG 140 1.6547347E-8 30.535711 4 CAGAAAA 70 1.6816557E-8 30.535711 10-11 CAAGTTG 35 0.008365001 27.142855 30-31 >>END_MODULE